A high-performance ammonia(NH_(3))sensor is prepared based on CeO_(2)/NiO composite,using a hydrothermal method.Experimental findings confirm that the CeO_(2)/NiO composite significantly enhances the performance of th...A high-performance ammonia(NH_(3))sensor is prepared based on CeO_(2)/NiO composite,using a hydrothermal method.Experimental findings confirm that the CeO_(2)/NiO composite significantly enhances the performance of the NiO-based NH_(3) sensor.This improvement is primarily due to the increase in oxygen vacancies(Ov),chemically adsorbed oxygen(Oc),and the proportion of Ni^(3+) on the surface of the CeO_(2)/NiO.The CeO_(2)/NiO sensor shows a high response to NH_(3),exhibiting response/recovery times of 1.8 s/0.9 s at the NH_(3) concentration of 5×10^(−6)mL/m^(3),with the theoretical lowest detection limit of 98.651×10^(−9)mL/m^(3).Additionally,the CeO_(2)/NiO sensor has been successfully applied in the simulated detection of Helicobacter pylori infection,highlighting its significant research value and potential application prospects in biomedical diagnostics.展开更多
Objective:To evaluate the clinical efficacy of high-throughput real-time mass spectrometry detection technology for exhaled breath in the rapid diagnosis of pulmonary tuberculosis(PTB),providing a novel technological ...Objective:To evaluate the clinical efficacy of high-throughput real-time mass spectrometry detection technology for exhaled breath in the rapid diagnosis of pulmonary tuberculosis(PTB),providing a novel technological support for early screening and diagnosis of PTB.Methods:A total of 120 PTB patients admitted to a hospital from January 2023 to June 2024 were selected as the case group,and 150 healthy individuals and patients with non-tuberculous pulmonary diseases during the same period were selected as the control group.Exhaled breath samples were collected from all study subjects,and the types and concentrations of volatile organic compounds(VOCs)in the samples were detected using a high-throughput real-time mass spectrometer.A diagnostic model was constructed using machine learning algorithms,and core indicators such as diagnostic sensitivity,specificity,and area under the curve(AUC)of this technology were analyzed and compared with the efficacy of traditional sputum smear examination,sputum culture,and GeneXpert MTB/RIF detection.Results:The diagnostic sensitivity of the high-throughput real-time mass spectrometry diagnostic model for exhaled breath in diagnosing PTB was 92.5%,the specificity was 94.0%,and the AUC was 0.978,which were significantly higher than those of sputum smear examination(sensitivity 58.3%,specificity 90.0%,AUC 0.741).Compared with GeneXpert technology,its specificity was comparable(94.0%vs 93.3%),and the detection time was shortened to less than 15 minutes.The model achieved an accuracy of 91.3%in distinguishing PTB from other pulmonary diseases and was not affected by demographic factors such as age and gender.Conclusion:High-throughput real-time mass spectrometry detection technology for exhaled breath has the advantages of being non-invasive,rapid,highly sensitive,and highly specific,and holds significant clinical application value in the rapid diagnosis and large-scale screening of PTB,warranting further promotion.展开更多
The quantitative detection of biological metabolites is a crucial route for early diagnosis of human diseases.Exhaled ammonia(NH_(3)),originating from abnormal metabolism,is normally recognized as the biomarker for li...The quantitative detection of biological metabolites is a crucial route for early diagnosis of human diseases.Exhaled ammonia(NH_(3)),originating from abnormal metabolism,is normally recognized as the biomarker for liver and kidney lesions.Therefore,developing highly sensitive fluorescent sensing materials is expected to replace the traditional clinical blood tests and facilitate painless diagnosis and telemedicine for patients.How-ever,the weak interaction for ammonia and the small color switching range of fluorescence sensors become the most pressing problem at present.Herein,a porphyrin-based hydrogen-bonded organic framework(HOf-6)with abundant supermolecule interactions in the confined pore space is developed for highly sensitive ammonia detection.The strong interactions between ammonia and the framework greatly promote the electron rear-rangement and enhance the intensity of fluorescence,enabling HOF-6 to successfully achieve trace amounts of ammonia sensing with the limit detection of 0.2 ppm.With the ultrahigh selectivity for ammonia,HOF-6 can accurately determine the amount of ammonia in breath of patients,and the test results are highly consistent with blood ammonia levels.The tailor-made multiple interactions in the confined pore space provide an effective approach for highly sensitive ammonia detection,as well as brings good news to liver and kidney patients for non-invasive diagnosis and real-time health monitoring.展开更多
基金supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region of China“Preparation and application of self-powered carbon nitride/metal oxide humidity sensors”(2023D01C05).
文摘A high-performance ammonia(NH_(3))sensor is prepared based on CeO_(2)/NiO composite,using a hydrothermal method.Experimental findings confirm that the CeO_(2)/NiO composite significantly enhances the performance of the NiO-based NH_(3) sensor.This improvement is primarily due to the increase in oxygen vacancies(Ov),chemically adsorbed oxygen(Oc),and the proportion of Ni^(3+) on the surface of the CeO_(2)/NiO.The CeO_(2)/NiO sensor shows a high response to NH_(3),exhibiting response/recovery times of 1.8 s/0.9 s at the NH_(3) concentration of 5×10^(−6)mL/m^(3),with the theoretical lowest detection limit of 98.651×10^(−9)mL/m^(3).Additionally,the CeO_(2)/NiO sensor has been successfully applied in the simulated detection of Helicobacter pylori infection,highlighting its significant research value and potential application prospects in biomedical diagnostics.
基金Science and Technology Plan of Heilongjiang Provincial Health Commission,Study on the Efficacy of High-Throughput Real-Time Mass Spectrometry Detection of Exhaled Breath for Rapid Diagnosis of Pulmonary Tuberculosis(Project No.:20230303110014)。
文摘Objective:To evaluate the clinical efficacy of high-throughput real-time mass spectrometry detection technology for exhaled breath in the rapid diagnosis of pulmonary tuberculosis(PTB),providing a novel technological support for early screening and diagnosis of PTB.Methods:A total of 120 PTB patients admitted to a hospital from January 2023 to June 2024 were selected as the case group,and 150 healthy individuals and patients with non-tuberculous pulmonary diseases during the same period were selected as the control group.Exhaled breath samples were collected from all study subjects,and the types and concentrations of volatile organic compounds(VOCs)in the samples were detected using a high-throughput real-time mass spectrometer.A diagnostic model was constructed using machine learning algorithms,and core indicators such as diagnostic sensitivity,specificity,and area under the curve(AUC)of this technology were analyzed and compared with the efficacy of traditional sputum smear examination,sputum culture,and GeneXpert MTB/RIF detection.Results:The diagnostic sensitivity of the high-throughput real-time mass spectrometry diagnostic model for exhaled breath in diagnosing PTB was 92.5%,the specificity was 94.0%,and the AUC was 0.978,which were significantly higher than those of sputum smear examination(sensitivity 58.3%,specificity 90.0%,AUC 0.741).Compared with GeneXpert technology,its specificity was comparable(94.0%vs 93.3%),and the detection time was shortened to less than 15 minutes.The model achieved an accuracy of 91.3%in distinguishing PTB from other pulmonary diseases and was not affected by demographic factors such as age and gender.Conclusion:High-throughput real-time mass spectrometry detection technology for exhaled breath has the advantages of being non-invasive,rapid,highly sensitive,and highly specific,and holds significant clinical application value in the rapid diagnosis and large-scale screening of PTB,warranting further promotion.
基金supported by the National Natural Science Foundation of China(No.22278287)Fundamental Research Program of Shanxi Province(Nos.202203021223004,202203021222342)+2 种基金Science and Technology Cooperation and Exchange Special Project of Shanxi Province(202304041101048)Research and Innovation Team Project for Scientific Breakthroughs at Shanxi Bethune Hospital(2024ZHAN-CHI06)Science and Technology Achievements Transformation Guidance Special Project of Shanxi Province(202404021301057).
文摘The quantitative detection of biological metabolites is a crucial route for early diagnosis of human diseases.Exhaled ammonia(NH_(3)),originating from abnormal metabolism,is normally recognized as the biomarker for liver and kidney lesions.Therefore,developing highly sensitive fluorescent sensing materials is expected to replace the traditional clinical blood tests and facilitate painless diagnosis and telemedicine for patients.How-ever,the weak interaction for ammonia and the small color switching range of fluorescence sensors become the most pressing problem at present.Herein,a porphyrin-based hydrogen-bonded organic framework(HOf-6)with abundant supermolecule interactions in the confined pore space is developed for highly sensitive ammonia detection.The strong interactions between ammonia and the framework greatly promote the electron rear-rangement and enhance the intensity of fluorescence,enabling HOF-6 to successfully achieve trace amounts of ammonia sensing with the limit detection of 0.2 ppm.With the ultrahigh selectivity for ammonia,HOF-6 can accurately determine the amount of ammonia in breath of patients,and the test results are highly consistent with blood ammonia levels.The tailor-made multiple interactions in the confined pore space provide an effective approach for highly sensitive ammonia detection,as well as brings good news to liver and kidney patients for non-invasive diagnosis and real-time health monitoring.
