Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the trea...Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.展开更多
Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols...Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.展开更多
Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects ...Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.展开更多
This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dos...This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dose was found to significantly reduce the symptoms of food allergy(FA)in mice,lower the levels of histamine and allergen-specific(immunoglobulin E),and attenuate intestinal inflammation.Further studies demonstrated that RBP decreased allergy-associated responses in intestinal epithelial cells and Th2 cells while regulating Th1/Th2 differentiation to reach a dynamic equilibrium and downregulating Th2 polarization caused by allergies.Short-chain fatty acids(SCFAs)content and 16S rRNA sequencing revealed that RBP enhanced the abundance and diversity of the gut microflora and raised the content of SCFAs.These results suggest that RBP may mitigate FA by modulating the gut microbiota.This study revealed the effective use of rice bran and served as a basis for the development of innovative functional foods with anti-allergic properties.展开更多
A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Glirici...A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.展开更多
To find an effective method for ensiling peanut vine （PV）, fermentation characteristics and nutritional values of PV silage and the mixture of PV with corn stover （CS） silage in a ratio of 1 ： 1 fresh weight, pre...To find an effective method for ensiling peanut vine （PV）, fermentation characteristics and nutritional values of PV silage and the mixture of PV with corn stover （CS） silage in a ratio of 1 ： 1 fresh weight, prepared by adding lactic acid bacteria （LAB）, 10% wheat bran （WB） and LAB＋WB at ensiling were evaluated in 2009 and 2010. The fermentation qualities of PV silage ensiled with the LAB and WB additives were improved compared with those of the control （PV ensiled alone）. However, the pH did not decline to the critical level of 4.2, and the nutritional values of the silage were not protected against losses in the LAB and WB addition silages. Ensiling PV in mixture with CS generated optimal moisture content and buffering capacity （BC） of ensiled materials. After adding the LAB and WB additives to mixture silage, especially adding LAB＋WB, the fermentation qualities and nutritional values of the mixture silage were improved significantly （P〈0.05）, and the Flieg＇s score reached to 99. The result suggested that it is a feasible method to ensile the mixed materials of PV with CS by adding LAB and high concentration of water soluble-carbohydrate materials for providing a good fermentation quality of PV silage.展开更多
基金supported by the Science and Technology Innovation Program of Hunan Province(2022RC1148)the Natural Science Foundation of Hunan Province(2022JJ31009,2022JJ50260)+4 种基金the Program for Science and Technology of Changsha,China(kh2301028)the Science and Technology Innovation Plan Project of Hunan Province(2023NK2033)the Innovation Leading Plan Project of Hunan Province(2021GK4022)the“Kemen Food”Graduate Science and Technology Innovation Project of Central South University of Forestry and Technology(2023KMCX02)the Graduate Science and Technology Innovation Fund Project of Hunan Province(QL20220182).
文摘Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.
基金the support from the research grants by National Natural Science Foundation of China(32202051)National Key R&D Program of China(2022YFF1100104)the Major Project of Inner Mongolia Science and Technology Department,China(2021ZD0002)。
文摘Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.
基金supported by grants from the National Key R&D Program(2023YFD1301303)National Natural Science Foundation of China(32472950,U21A20249)+1 种基金China Agriculture Research System of MOF and MARA(CARS-35)National Center of Technology Innovation for Pigs,Zhejiang Agricultural Talents,Taishan Industrial Leading Talents Project.
文摘Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.
基金provided by the Shanghai Agricultural Science and Technology Innovation Program(Shanghai Agricultural Science I2023007)the Zhejiang Provincial Natural Science Foundation of China(LZ23C200001)+1 种基金Shanghai Agricultural Products Preservation and Processing Professional Technical Service Platform(21DZ2292200)Agri-food Storage and Processing Engineering Technology Research Center of Shanghai(19DZ2251600).
文摘This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dose was found to significantly reduce the symptoms of food allergy(FA)in mice,lower the levels of histamine and allergen-specific(immunoglobulin E),and attenuate intestinal inflammation.Further studies demonstrated that RBP decreased allergy-associated responses in intestinal epithelial cells and Th2 cells while regulating Th1/Th2 differentiation to reach a dynamic equilibrium and downregulating Th2 polarization caused by allergies.Short-chain fatty acids(SCFAs)content and 16S rRNA sequencing revealed that RBP enhanced the abundance and diversity of the gut microflora and raised the content of SCFAs.These results suggest that RBP may mitigate FA by modulating the gut microbiota.This study revealed the effective use of rice bran and served as a basis for the development of innovative functional foods with anti-allergic properties.
文摘A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.
基金funded by the National Key Technology Research and Development Program of China(2009BADA6B06)
文摘To find an effective method for ensiling peanut vine （PV）, fermentation characteristics and nutritional values of PV silage and the mixture of PV with corn stover （CS） silage in a ratio of 1 ： 1 fresh weight, prepared by adding lactic acid bacteria （LAB）, 10% wheat bran （WB） and LAB＋WB at ensiling were evaluated in 2009 and 2010. The fermentation qualities of PV silage ensiled with the LAB and WB additives were improved compared with those of the control （PV ensiled alone）. However, the pH did not decline to the critical level of 4.2, and the nutritional values of the silage were not protected against losses in the LAB and WB addition silages. Ensiling PV in mixture with CS generated optimal moisture content and buffering capacity （BC） of ensiled materials. After adding the LAB and WB additives to mixture silage, especially adding LAB＋WB, the fermentation qualities and nutritional values of the mixture silage were improved significantly （P〈0.05）, and the Flieg＇s score reached to 99. The result suggested that it is a feasible method to ensile the mixed materials of PV with CS by adding LAB and high concentration of water soluble-carbohydrate materials for providing a good fermentation quality of PV silage.
