Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the trea...Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.展开更多
Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols...Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.展开更多
Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects ...Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.展开更多
This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dos...This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dose was found to significantly reduce the symptoms of food allergy(FA)in mice,lower the levels of histamine and allergen-specific(immunoglobulin E),and attenuate intestinal inflammation.Further studies demonstrated that RBP decreased allergy-associated responses in intestinal epithelial cells and Th2 cells while regulating Th1/Th2 differentiation to reach a dynamic equilibrium and downregulating Th2 polarization caused by allergies.Short-chain fatty acids(SCFAs)content and 16S rRNA sequencing revealed that RBP enhanced the abundance and diversity of the gut microflora and raised the content of SCFAs.These results suggest that RBP may mitigate FA by modulating the gut microbiota.This study revealed the effective use of rice bran and served as a basis for the development of innovative functional foods with anti-allergic properties.展开更多
Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and ...Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.展开更多
Inflammatory bowel disease(IBD)is a chronic relapsing-remitting systemic disease of the gastrointestinal tract,characterized by an inflammatory process.Gut mycobiota community dysbiosis has been reported that is close...Inflammatory bowel disease(IBD)is a chronic relapsing-remitting systemic disease of the gastrointestinal tract,characterized by an inflammatory process.Gut mycobiota community dysbiosis has been reported that is closely related to the development of IBD.Our previous findings indicated that polyphenol of the inner shell(BPIS)from foxtail millet bran could restore the gut microbiome and inhibit the progress of colorectal cancer(CRC).In the present study,we studied the anti-inflammatory potential of BPIS in the dextran sodium sulfate(DSS)-induced mouse colitis model.Data suggested that BPIS alleviated experimental colitis by restoring body weight,colonic length and protecting the epithelial architecture from damage by DSS.Moreover,we found that BPIS strengthened the gut barrier function and inhibited the activation of Wnt1/β-catenin pathway.Gene sequence analysis indicated that BPIS remodeled the overall structure of the gut mycobiota from colitis mice toward that of the normal counterparts,including 1 phylum and 9 genera.Interestingly,BPIS significantly increased the abundance of Aspergillus ruber.It further verified that BPIS significantly promoted the growth of A.ruber in vitro.Collectively,BPIS has great potential to develop into an effective against IBD drug.展开更多
Wheat bran, a principal byproduct of flour milling, stands as an abundant source of dietary fiber, yet its economic potential remains under-exploited in current forage applications. Arabinoxylan(AX), constituting the ...Wheat bran, a principal byproduct of flour milling, stands as an abundant source of dietary fiber, yet its economic potential remains under-exploited in current forage applications. Arabinoxylan(AX), constituting the core of dietary fiber, emerges as a versatile compound with multifaceted functionalities. Its nutritional significance,coupled with its role in cereal food processing, has prompted a surge of studies focusing on the valorization of wheat bran AX. Moreover, the hydrolyzed derivative, arabinoxylan oligosaccharides(AXOS), demonstrates prebiotic and antioxidant properties, offering potential avenues to mitigate the risk of chronic diseases. This review summarizes current knowledge on the valorization of wheat bran AX in terms of the processing and nutritional properties of AX. Moreover, multiple novel applications of AX in the materials area, including biodegradable food packaging films, delivery of bioactive substances as nanoparticles, and the manufacture of food emulsifiers, are also highlighted to extend the utilization of AX. This review underscores the immense potential of wheat bran AX, advocating for its exploitation not only as a nutritional asset but also as a primary ingredient in advanced materials. The synthesis of nutritional and materials perspectives accentuates the multifaceted utility of wheat bran AX, thereby paving the way for sustainable valorization pathways. By unraveling the latent potential within AX, this paper advocates for the holistic and sustainable utilization of wheat bran in diverse, value-added applications.展开更多
[Objectives]This study was conducted to investigate the process conditions,function and structural characteristics of oat bran dietary fiber prepared by steam explosion(SE).[Methods]With oat bran as the raw material,t...[Objectives]This study was conducted to investigate the process conditions,function and structural characteristics of oat bran dietary fiber prepared by steam explosion(SE).[Methods]With oat bran as the raw material,the technical parameters for preparing dietary fiber by steam explosion were studied,and the functional and structural characteristics of DF before and after modification were discussed.[Results]The optimum conditions for extracting DF from oat bran by SE modification were steam explosion pressure of 0.6 MPa and holding time of 4 min.The extraction rate of DF reached 33.9%.The solubility,water holding capacity,oil holding capacity and swelling force of Control-DF were 78.35%,2.25 g/g,1.55 g/g and 3.05 ml/g,respectively,and those of SE-DF were 95.69%,3.28 g/g,2.18 g/g and 5.98 ml/g,respectively.After SE treatment,the scavenging rates of oat bran DF on DPPH,ABTS,O-2·and·OH were significantly higher than those of untreated samples.The scavenging ability on free radicals was enhanced.The scavenging rates of Control-DF on DPPH,ABTS,O-2·and·OH were 43.72%,50.26%,31.02%and 39.25%,respectively,and those of SE-DF were 70.25%,73.21%and 63.69%59.32%,respectively.The surface of modified DF showed an obvious honeycomb structure.[Conclusions]This study can provide reference for functional modifications and utilization of dietary fiber from oat bran.展开更多
基金supported by the Science and Technology Innovation Program of Hunan Province(2022RC1148)the Natural Science Foundation of Hunan Province(2022JJ31009,2022JJ50260)+4 种基金the Program for Science and Technology of Changsha,China(kh2301028)the Science and Technology Innovation Plan Project of Hunan Province(2023NK2033)the Innovation Leading Plan Project of Hunan Province(2021GK4022)the“Kemen Food”Graduate Science and Technology Innovation Project of Central South University of Forestry and Technology(2023KMCX02)the Graduate Science and Technology Innovation Fund Project of Hunan Province(QL20220182).
文摘Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.
基金the support from the research grants by National Natural Science Foundation of China(32202051)National Key R&D Program of China(2022YFF1100104)the Major Project of Inner Mongolia Science and Technology Department,China(2021ZD0002)。
文摘Whole-grain foods have attracted emerging attention due to their health benefits.Whole grains are rich in bound polyphenols(BPs)linked with dietary fibers,which is largely underestimated compared with free polyphenols.In this study,in vitro simulated gastrointestinal digestion and colonic fermentation models were used to study the release profile and metabolism of BPs of oat bran.Significantly higher level of BPs was released during in vitro colon fermentation(3.05 mg GAE/g)than in gastrointestinal digestion(0.54 mg GAE/g).Five polyphenols were detected via LC-MS and their possible conversion pathways were speculated.Released BPs exhibited chemical antioxidant capacity.16S rRNA sequencing further revealed that Clostridium butyricum,Enterococcus faecalis,Bacteroides acidifaciens were the key bacteria involved in the release of BPs,and this was verified by whole-cell transformation.Our results helped to explain the possible mechanism of the health benefits of BPs in whole grains.
基金supported by grants from the National Key R&D Program(2023YFD1301303)National Natural Science Foundation of China(32472950,U21A20249)+1 种基金China Agriculture Research System of MOF and MARA(CARS-35)National Center of Technology Innovation for Pigs,Zhejiang Agricultural Talents,Taishan Industrial Leading Talents Project.
文摘Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.
基金provided by the Shanghai Agricultural Science and Technology Innovation Program(Shanghai Agricultural Science I2023007)the Zhejiang Provincial Natural Science Foundation of China(LZ23C200001)+1 种基金Shanghai Agricultural Products Preservation and Processing Professional Technical Service Platform(21DZ2292200)Agri-food Storage and Processing Engineering Technology Research Center of Shanghai(19DZ2251600).
文摘This study is the first investigation of the effects of polysaccharide extracted from rice bran(RBP)on ovalbumin(OVA)-induced allergy and the gut microbiota,which hasn’t been reported previously.RBP in the medium-dose was found to significantly reduce the symptoms of food allergy(FA)in mice,lower the levels of histamine and allergen-specific(immunoglobulin E),and attenuate intestinal inflammation.Further studies demonstrated that RBP decreased allergy-associated responses in intestinal epithelial cells and Th2 cells while regulating Th1/Th2 differentiation to reach a dynamic equilibrium and downregulating Th2 polarization caused by allergies.Short-chain fatty acids(SCFAs)content and 16S rRNA sequencing revealed that RBP enhanced the abundance and diversity of the gut microflora and raised the content of SCFAs.These results suggest that RBP may mitigate FA by modulating the gut microbiota.This study revealed the effective use of rice bran and served as a basis for the development of innovative functional foods with anti-allergic properties.
基金supported by the State Administration for Market Regulation Science and Technology Plan Project under Grant 2022MK192.
文摘Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.
基金supported by the National Natural Science Foundation of China(U23A20526,32072220,32270420)Shanxi Province 136 Revitalization Medical Project Construction Funds(general surgery department),Science Foundation(202103021224011).
文摘Inflammatory bowel disease(IBD)is a chronic relapsing-remitting systemic disease of the gastrointestinal tract,characterized by an inflammatory process.Gut mycobiota community dysbiosis has been reported that is closely related to the development of IBD.Our previous findings indicated that polyphenol of the inner shell(BPIS)from foxtail millet bran could restore the gut microbiome and inhibit the progress of colorectal cancer(CRC).In the present study,we studied the anti-inflammatory potential of BPIS in the dextran sodium sulfate(DSS)-induced mouse colitis model.Data suggested that BPIS alleviated experimental colitis by restoring body weight,colonic length and protecting the epithelial architecture from damage by DSS.Moreover,we found that BPIS strengthened the gut barrier function and inhibited the activation of Wnt1/β-catenin pathway.Gene sequence analysis indicated that BPIS remodeled the overall structure of the gut mycobiota from colitis mice toward that of the normal counterparts,including 1 phylum and 9 genera.Interestingly,BPIS significantly increased the abundance of Aspergillus ruber.It further verified that BPIS significantly promoted the growth of A.ruber in vitro.Collectively,BPIS has great potential to develop into an effective against IBD drug.
基金supported by the National Key Research and Development Plan Project (2022YFD2301401)Young Elite Scientists Sponsorship Program by the CAST (2022QNRC001)+4 种基金the Outstanding Youth Science Fund Project of Natural Science Foundation of Jiangsu Province (BK20211576)the Central Government Guides Local Funds (ZYYD2023A13)Key Technology Research and Development Program of Jiangsu Province (BE2023370)Hainan Province (ZDYF2022XDNY233)a project funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions。
文摘Wheat bran, a principal byproduct of flour milling, stands as an abundant source of dietary fiber, yet its economic potential remains under-exploited in current forage applications. Arabinoxylan(AX), constituting the core of dietary fiber, emerges as a versatile compound with multifaceted functionalities. Its nutritional significance,coupled with its role in cereal food processing, has prompted a surge of studies focusing on the valorization of wheat bran AX. Moreover, the hydrolyzed derivative, arabinoxylan oligosaccharides(AXOS), demonstrates prebiotic and antioxidant properties, offering potential avenues to mitigate the risk of chronic diseases. This review summarizes current knowledge on the valorization of wheat bran AX in terms of the processing and nutritional properties of AX. Moreover, multiple novel applications of AX in the materials area, including biodegradable food packaging films, delivery of bioactive substances as nanoparticles, and the manufacture of food emulsifiers, are also highlighted to extend the utilization of AX. This review underscores the immense potential of wheat bran AX, advocating for its exploitation not only as a nutritional asset but also as a primary ingredient in advanced materials. The synthesis of nutritional and materials perspectives accentuates the multifaceted utility of wheat bran AX, thereby paving the way for sustainable valorization pathways. By unraveling the latent potential within AX, this paper advocates for the holistic and sustainable utilization of wheat bran in diverse, value-added applications.
文摘[Objectives]This study was conducted to investigate the process conditions,function and structural characteristics of oat bran dietary fiber prepared by steam explosion(SE).[Methods]With oat bran as the raw material,the technical parameters for preparing dietary fiber by steam explosion were studied,and the functional and structural characteristics of DF before and after modification were discussed.[Results]The optimum conditions for extracting DF from oat bran by SE modification were steam explosion pressure of 0.6 MPa and holding time of 4 min.The extraction rate of DF reached 33.9%.The solubility,water holding capacity,oil holding capacity and swelling force of Control-DF were 78.35%,2.25 g/g,1.55 g/g and 3.05 ml/g,respectively,and those of SE-DF were 95.69%,3.28 g/g,2.18 g/g and 5.98 ml/g,respectively.After SE treatment,the scavenging rates of oat bran DF on DPPH,ABTS,O-2·and·OH were significantly higher than those of untreated samples.The scavenging ability on free radicals was enhanced.The scavenging rates of Control-DF on DPPH,ABTS,O-2·and·OH were 43.72%,50.26%,31.02%and 39.25%,respectively,and those of SE-DF were 70.25%,73.21%and 63.69%59.32%,respectively.The surface of modified DF showed an obvious honeycomb structure.[Conclusions]This study can provide reference for functional modifications and utilization of dietary fiber from oat bran.
