A novel caged-prenylxanthone xanthone,neobractatin(1),was isolated from the twig of Garcinia bracteata.Its structure was elucidated by spectroscopic methods,especially 2D NMR techniques.
Objective:The bulb of Albuca bracteata Jacq.,and Albuca setosa commonly used in Eastern Cape Province of South Africa for the treatment of several types of disease conditions including diabetes,was investigated for th...Objective:The bulb of Albuca bracteata Jacq.,and Albuca setosa commonly used in Eastern Cape Province of South Africa for the treatment of several types of disease conditions including diabetes,was investigated for their phytochemical contents and antioxidant activities.Methods:The antioxidant activity was determined by evaluating the effect of various solvent extracts(acetone,methanol,ethanol and water) on 2,2 – diphenyl-2-picrylhydrazyl(DPPH) scavenging activity,2,20– azino-bis(3-ethylbenzthiazoline-6-sulfonic acid diammonium salt(ABTS),ferric reducing power,hydrogen peroxide and nitric oxide scavenging activity while the various extracts were also analysed for phytochemical their contents.Results:The results obtained indicate that polyphenols,proanthocyanidins,saponins and alkaloids are present in higher concentrations.The composition of phenols(117 mg/g),flavonols(26.28 mg/g),proanthocyanidins(84.85 mg/g) and flavonoids(5.36 mg/g) were significantly higher in the acetone extract while saponin and alkaloids contents were higher in the aqueous extract.Antioxidant studies of the extracts for nitric oxide,hydrogen peroxide,ABTS and DPPH showed high antioxidant potential in a concentration-dependent manner that was not significantly different from Vitamin C,BHT and Rutin used as standard references.The methanol extract showed higher ferric reducing potential compared to the aqueous extract but was significantly different from Vitamin C,BHT and Rutin.Conclusion:This implies that the bulb of Albuca bracteata may serve as a natural source of antioxidants,which together with the numerous phytochemicals present,could account for its folkloric use as a medicinal plant.展开更多
Chromosome studies and soluble protein profiles, fractionated by reducing and non-reducing SDS-PAGE, were carried out in dioecious Trichosanthes bracteata. Somatic chromosome no. 2n = 22 was recorded in both sexes. Th...Chromosome studies and soluble protein profiles, fractionated by reducing and non-reducing SDS-PAGE, were carried out in dioecious Trichosanthes bracteata. Somatic chromosome no. 2n = 22 was recorded in both sexes. The karyotype of male and female plant shows high homogeneity and the absence of any heteromorphic pair of chromosomes negates the possibility of XY mechanism. Soluble protein profiles from the tuberous roots of the male and female plants, fractionated by reducing SDS-PAGE, did not show any qualitative distinction. Whereas the protein profile in non-reducing SDS-PAGE reveals a clear distinction when compared on a single gel. The difference is marked by the presence of a disulphide linked tertiary or folded protein at 19 k D region detected in male sex. However, at the level of primary structure the qualitative expression is similar indicating a common ancestry.展开更多
This study examines the ability of Mucuna bracteata DC. to remediate soil contaminated with increasing levels of crude oil up to 20%. It also investigates the effect of fertilizer application on crude oil degradation....This study examines the ability of Mucuna bracteata DC. to remediate soil contaminated with increasing levels of crude oil up to 20%. It also investigates the effect of fertilizer application on crude oil degradation. Changes in crude oil concentrations, pH and moisture of the soil in eight experimental pots were tracked over a period of 9 weeks. The crude oil levels in soil were analysed as Total Petroleum Hydrocarbons (TPHs) using the UV-Vis spectrophotometer. The study revealed the capacity of Mucuna bracteata to phytoremediate soil contaminated with crude oil in all experimental pots though the plant died at 20% contamination towards the end of the experiment. The plant survived up to 15% contamination with that in the fertilized pot showing better physiological conditions. In all instances, fertilized pots showed higher rates of crude oil reduction. The amounts of crude oil degraded in fertilized pots were also higher except at 20% contamination. The soil pH varied over a narrow range throughout the experimental period. Moisture of soil contaminated with 15% and 20% crude oil was higher than that contaminated with 5% and 10% crude oil. Mucuna bracteata showed signs of phytoextraction which can be subject to further study. This study contributed a new candidate of phytoremediation for soil contaminated with high level of crude oil.展开更多
The flavonoid 4’,5,7-trihydroxy-6,8-dimethylflavanone (farresol) and 3’,4’,-4trihydroxy chalcone (isoliquiritigenin) together with triterpenoid (friedelin) were isolated from the aerial parts of P. bracteata. The s...The flavonoid 4’,5,7-trihydroxy-6,8-dimethylflavanone (farresol) and 3’,4’,-4trihydroxy chalcone (isoliquiritigenin) together with triterpenoid (friedelin) were isolated from the aerial parts of P. bracteata. The structure of these compounds was determined on the basis of spectroscopic data including UV, IR, 1-D and 2-D, NMR and mass spectral analysis. This is the first report on the occurrence of these compounds in this plant.展开更多
Objective: To investigate the mechanisms that underlie the anti-asthmatic effects of Nepeta bracteata(DBJJ, Dabao Jingjie in Chinese) in rats by integrating metabolomics and network pharmacology.Methods: In this study...Objective: To investigate the mechanisms that underlie the anti-asthmatic effects of Nepeta bracteata(DBJJ, Dabao Jingjie in Chinese) in rats by integrating metabolomics and network pharmacology.Methods: In this study, the rat model of asthma was induced by ovalbumin(OVA), and the rats were treated with a decoction of N. bracteata. Pathological changes in lung tissue were observed, and the quantification of eosinophils(EOS) and white blood cells(WBC) in bronchoalveolar lavage fluid was performed.Furthermore, the serum levels of asthma-related factors induced by OVA were assessed.^(1)H NMR spectroscopy serum metabolomics method was utilized to identify differential metabolites and their associated metabolic pathways. UPLC-QE-MS/MS combined with network pharmacology was employed to predict the core targets and pathways of DBJJ in its action against asthma. The anti-asthmatic properties of DBJJ were investigated using an integrated approach of metabolomics and network pharmacology. The findings were validated through molecular docking and Western blotting analysis of the key targets.Results: The administration of DBJJ effectively alleviated OVA-induced lung histopathological changes and decreased the number of EOS and WBC in BALF. Additionally, DBJJ inhibited the OVA-induced elevation of TNF-a, IL-18, Ig-E, EOS, IL-1β, MDA, VEGF-A, and TGF-β1. A total of 21 biomarkers and 10 pathways were found by metabolomics analysis. A total of 29 compounds were identified by UPLC-QE-MS/MS, in which 13 active components were screened by oral availability and Caco-2 cell permeability, the 120 targets and 173 KEGG pathways were predicted. The integration of metabolomics and network pharmacological analysis revealed that DBJJ's main constituents, including ferulic acid and ursolic acid, exerted their effects on four targets, namely DAO and NOS2, as well as their associated metabolites and pathways.The active constituents of DBJJ demonstrated a high binding affinity towards DAO and NOS2.Furthermore, DBJJ was observed to decrease the protein expression and phosphorylation levels of NOS2, MAPK, and STAT3.Conclusion: The administration of DBJJ demonstrates notable anti-asthma properties in rats with allergic asthma. This effect can be attributed to the modulation of various targets, including NOS2, MAPK, and STAT3, by primary constituents such as ferulic acid and ursolic acid.展开更多
基金support of this work by the National Natural Science Foundation of China (No.20702061)
文摘A novel caged-prenylxanthone xanthone,neobractatin(1),was isolated from the twig of Garcinia bracteata.Its structure was elucidated by spectroscopic methods,especially 2D NMR techniques.
基金Govan Mbeki Research Development Council(GMRDC),University of Fort Hare(Grant No.C228)National Research Foundation(NRF)(Grant No.95187)both of South Africa for funding this work
文摘Objective:The bulb of Albuca bracteata Jacq.,and Albuca setosa commonly used in Eastern Cape Province of South Africa for the treatment of several types of disease conditions including diabetes,was investigated for their phytochemical contents and antioxidant activities.Methods:The antioxidant activity was determined by evaluating the effect of various solvent extracts(acetone,methanol,ethanol and water) on 2,2 – diphenyl-2-picrylhydrazyl(DPPH) scavenging activity,2,20– azino-bis(3-ethylbenzthiazoline-6-sulfonic acid diammonium salt(ABTS),ferric reducing power,hydrogen peroxide and nitric oxide scavenging activity while the various extracts were also analysed for phytochemical their contents.Results:The results obtained indicate that polyphenols,proanthocyanidins,saponins and alkaloids are present in higher concentrations.The composition of phenols(117 mg/g),flavonols(26.28 mg/g),proanthocyanidins(84.85 mg/g) and flavonoids(5.36 mg/g) were significantly higher in the acetone extract while saponin and alkaloids contents were higher in the aqueous extract.Antioxidant studies of the extracts for nitric oxide,hydrogen peroxide,ABTS and DPPH showed high antioxidant potential in a concentration-dependent manner that was not significantly different from Vitamin C,BHT and Rutin used as standard references.The methanol extract showed higher ferric reducing potential compared to the aqueous extract but was significantly different from Vitamin C,BHT and Rutin.Conclusion:This implies that the bulb of Albuca bracteata may serve as a natural source of antioxidants,which together with the numerous phytochemicals present,could account for its folkloric use as a medicinal plant.
文摘Chromosome studies and soluble protein profiles, fractionated by reducing and non-reducing SDS-PAGE, were carried out in dioecious Trichosanthes bracteata. Somatic chromosome no. 2n = 22 was recorded in both sexes. The karyotype of male and female plant shows high homogeneity and the absence of any heteromorphic pair of chromosomes negates the possibility of XY mechanism. Soluble protein profiles from the tuberous roots of the male and female plants, fractionated by reducing SDS-PAGE, did not show any qualitative distinction. Whereas the protein profile in non-reducing SDS-PAGE reveals a clear distinction when compared on a single gel. The difference is marked by the presence of a disulphide linked tertiary or folded protein at 19 k D region detected in male sex. However, at the level of primary structure the qualitative expression is similar indicating a common ancestry.
文摘This study examines the ability of Mucuna bracteata DC. to remediate soil contaminated with increasing levels of crude oil up to 20%. It also investigates the effect of fertilizer application on crude oil degradation. Changes in crude oil concentrations, pH and moisture of the soil in eight experimental pots were tracked over a period of 9 weeks. The crude oil levels in soil were analysed as Total Petroleum Hydrocarbons (TPHs) using the UV-Vis spectrophotometer. The study revealed the capacity of Mucuna bracteata to phytoremediate soil contaminated with crude oil in all experimental pots though the plant died at 20% contamination towards the end of the experiment. The plant survived up to 15% contamination with that in the fertilized pot showing better physiological conditions. In all instances, fertilized pots showed higher rates of crude oil reduction. The amounts of crude oil degraded in fertilized pots were also higher except at 20% contamination. The soil pH varied over a narrow range throughout the experimental period. Moisture of soil contaminated with 15% and 20% crude oil was higher than that contaminated with 5% and 10% crude oil. Mucuna bracteata showed signs of phytoextraction which can be subject to further study. This study contributed a new candidate of phytoremediation for soil contaminated with high level of crude oil.
文摘The flavonoid 4’,5,7-trihydroxy-6,8-dimethylflavanone (farresol) and 3’,4’,-4trihydroxy chalcone (isoliquiritigenin) together with triterpenoid (friedelin) were isolated from the aerial parts of P. bracteata. The structure of these compounds was determined on the basis of spectroscopic data including UV, IR, 1-D and 2-D, NMR and mass spectral analysis. This is the first report on the occurrence of these compounds in this plant.
基金financially supported by Xinjiang Uygur Autonomous Region Department of Science and Technology (No. 2016A03005-1)。
文摘Objective: To investigate the mechanisms that underlie the anti-asthmatic effects of Nepeta bracteata(DBJJ, Dabao Jingjie in Chinese) in rats by integrating metabolomics and network pharmacology.Methods: In this study, the rat model of asthma was induced by ovalbumin(OVA), and the rats were treated with a decoction of N. bracteata. Pathological changes in lung tissue were observed, and the quantification of eosinophils(EOS) and white blood cells(WBC) in bronchoalveolar lavage fluid was performed.Furthermore, the serum levels of asthma-related factors induced by OVA were assessed.^(1)H NMR spectroscopy serum metabolomics method was utilized to identify differential metabolites and their associated metabolic pathways. UPLC-QE-MS/MS combined with network pharmacology was employed to predict the core targets and pathways of DBJJ in its action against asthma. The anti-asthmatic properties of DBJJ were investigated using an integrated approach of metabolomics and network pharmacology. The findings were validated through molecular docking and Western blotting analysis of the key targets.Results: The administration of DBJJ effectively alleviated OVA-induced lung histopathological changes and decreased the number of EOS and WBC in BALF. Additionally, DBJJ inhibited the OVA-induced elevation of TNF-a, IL-18, Ig-E, EOS, IL-1β, MDA, VEGF-A, and TGF-β1. A total of 21 biomarkers and 10 pathways were found by metabolomics analysis. A total of 29 compounds were identified by UPLC-QE-MS/MS, in which 13 active components were screened by oral availability and Caco-2 cell permeability, the 120 targets and 173 KEGG pathways were predicted. The integration of metabolomics and network pharmacological analysis revealed that DBJJ's main constituents, including ferulic acid and ursolic acid, exerted their effects on four targets, namely DAO and NOS2, as well as their associated metabolites and pathways.The active constituents of DBJJ demonstrated a high binding affinity towards DAO and NOS2.Furthermore, DBJJ was observed to decrease the protein expression and phosphorylation levels of NOS2, MAPK, and STAT3.Conclusion: The administration of DBJJ demonstrates notable anti-asthma properties in rats with allergic asthma. This effect can be attributed to the modulation of various targets, including NOS2, MAPK, and STAT3, by primary constituents such as ferulic acid and ursolic acid.
