Background India harbors the world’s largest cattle population,encompassing over 50 distinct Bos indicus breeds.This rich genetic diversity underscores the inadequacy of a single reference genome to fully capture the...Background India harbors the world’s largest cattle population,encompassing over 50 distinct Bos indicus breeds.This rich genetic diversity underscores the inadequacy of a single reference genome to fully capture the genomic landscape of Indian cattle.To comprehensively characterize the genomic variation within Bos indicus and,specifically,dairy breeds,we aim to identify non-reference sequences and construct a comprehensive pangenome.Results Five representative genomes of prominent dairy breeds,including Gir,Kankrej,Tharparkar,Sahiwal,and Red Sindhi,were sequenced using 10X Genomics‘linked-read’technology.Assemblies generated from these linked-reads ranged from 2.70 Gb to 2.77 Gb,comparable to the Bos indicus Brahman reference genome.A pangenome of Bos indicus cattle was constructed by comparing the newly assembled genomes with the reference using alignment and graph-based methods,revealing 8 Mb and 17.7 Mb of novel sequence respectively.A confident set of 6,844 Non-reference Unique Insertions(NUIs)spanning 7.57 Mb was identified through both methods,representing the pange-nome of Indian Bos indicus breeds.Comparative analysis with previously published pangenomes unveiled 2.8 Mb(37%)commonality with the Chinese indicine pangenome and only 1%commonality with the Bos taurus pange-nome.Among these,2,312 NUIs encompassing~2 Mb,were commonly found in 98 samples of the 5 breeds and des-ignated as Bos indicus Common Insertions(BICIs)in the population.Furthermore,926 BICIs were identified within 682 protein-coding genes,54 long non-coding RNAs(lncRNA),and 18 pseudogenes.These protein-coding genes were enriched for functions such as chemical synaptic transmission,cell junction organization,cell-cell adhesion,and cell morphogenesis.The protein-coding genes were found in various prominent quantitative trait locus(QTL)regions,suggesting potential roles of BICIs in traits related to milk production,reproduction,exterior,health,meat,and carcass.Notably,63.21%of the bases within the BICIs call set contained interspersed repeats,predominantly Long Inter-spersed Nuclear Elements(LINEs).Additionally,70.28%of BICIs are shared with other domesticated and wild species,highlighting their evolutionary significance.Conclusions This is the first report unveiling a robust set of NUIs defining the pangenome of Bos indicus breeds of India.The analyses contribute valuable insights into the genomic landscape of desi cattle breeds.展开更多
为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和...为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)技术,检测了甘南牦牛FBXO32基因突变位点及基因型,分析了基因型与甘南牦牛胴体及肉质性状的相关性。结果表明,从甘南牦牛FBXO32基因检测到7个SNP位点,分别是位于5′UTR区的SNP1(g.267A>C)、外显子1区的SNP2(g.326G>T)、外显子8区的SNP3(g.31231G>C),以及3′UTR区的SNP4(g.31352G>A)、SNP5(g.31424C>T)、SNP6(g.31503A>C)和SNP7(g.31504A>G)。其中,SNP1、SNP4、SNP5、SNP6与肌肉嫩度显著相关(P<0.05),SNP3、SNP6、SNP7与失水率显著相关(P<0.05),SNP1、SNP2、SNP3、SNP4、SNP5、SNP7与眼肌面积显著相关(P<0.05),SNP2、SNP5、SNP6与胴体重显著相关(P<0.05)。7个SNP位点构建的9种有效单倍型组合中,单倍型组合H2H2的个体肌肉嫩度小、熟肉率高,单倍型组合H1H2的个体熟肉率高、眼肌面积大、失水率低。FBXO32基因突变位点可作为甘南牦牛胴体及肉质性状潜在分子遗传标记,研究结果丰富了甘南牦牛经济性状分子遗传理论基础。展开更多
Boscalid is a pesticide with the advantages of broad spectrum bactericidal activity,high efficiency,low toxicity,and no cross-resistance with other fungicides currently available on the market.Herein,we report the syn...Boscalid is a pesticide with the advantages of broad spectrum bactericidal activity,high efficiency,low toxicity,and no cross-resistance with other fungicides currently available on the market.Herein,we report the synthesis of 40-chloro-2-nitrobiphenyl,a key intermediate of Boscalid using a palladium-catalyzed Suzuki-Miyaura cross-coupling employing the 2-aryl-substituted indenyl phosphine ligand.40-Chloro-2-nitrobiphenyl was prepared in 94%yield on a 100 g scale.This method allows for the industrial production of alimide and active substances bearing a biphenyl moiety.展开更多
[Objective]The paper was to provide reference for further study and development of gayal（Bos frontalis）.[Method]According to the research status at home and abroad,the biological characteristics and rumen microorgan...[Objective]The paper was to provide reference for further study and development of gayal（Bos frontalis）.[Method]According to the research status at home and abroad,the biological characteristics and rumen microorganisms of gayal in Yunnan Province was studied.[Result]Gayal in Yunnan had typical body form and very good meat production performance,its muscle fiber diameter was significantly less than other kinds of cattle;the water holding ratio,muscle tenderness and muscle succulency were significantly higher than others;its amount,shape and structure of chromosome were different from yellow cattle（Bos taurus）and wild cattle（Bos gaurus）,and the amounts of those chromosomes（2n）were 58,60 and 56,respectively.It could create hybrid with yellow cattle;the gayal＇s special diet was bamboo,its in vitro dry matter digestibility（IVDMD）on various crude forage was significantly higher than yellow cattle in Yunnan;the viable bacteria and cellulolytic bacteria in rumen were 4.51×109 and 1.63×109 CFU/ml,which was significantly higher than yellow cattle in Yunnan,its dominant bacteria in rumen mainly was cellulolytic bacteria.[Conclusion]Gayal not only had high academic value,but also had a great development value.展开更多
[目的]本文旨在研究超高压协同温度处理对过敏原Bos d 6抗原性和二级结构的影响。[方法]采用间接竞争ELISA和圆二色光谱方法,研究在25、40和55℃条件下,100、200、300、400、500和600 MPa压力分别处理10 min对过敏原Bos d 6抗原性及二...[目的]本文旨在研究超高压协同温度处理对过敏原Bos d 6抗原性和二级结构的影响。[方法]采用间接竞争ELISA和圆二色光谱方法,研究在25、40和55℃条件下,100、200、300、400、500和600 MPa压力分别处理10 min对过敏原Bos d 6抗原性及二级结构的影响,并分析过敏原抗原性与其二级结构中α-螺旋的相关性。[结果]超高压处理能有效降低过敏原Bos d 6的抗原性及其二级结构中α-螺旋含量;随着处理温度的增加,过敏原抗原性和α-螺旋含量的降低幅度明显;超高压协同温度处理后过敏原Bos d 6抗原性与α-螺旋含量呈明显的正相关性。[结论]超高压协同温度处理技术可以通过改变过敏原Bos d 6蛋白质的空间构象来达到降低其抗原性的目的。展开更多
To date,sperm morphometric studies have assessed whole sperm populations without considering sperm function.The aim of this study was to evaluate the relati on ship of sperm membrane and acrosomal integrity with sperm...To date,sperm morphometric studies have assessed whole sperm populations without considering sperm function.The aim of this study was to evaluate the relati on ship of sperm membrane and acrosomal integrity with sperm morphometry in liquid semen samples collected from bulls.To this end,sperm morphometry was performed on cryopreserved semen samples from 16 bulls by a combination of fluorescent dyes,including Hoechst 33343,carboxyfluorescein diacetate,and propidium iodide.This allowed discrimination of different subpopulations on the basis of sperm membrane and acrosomal integrity and analysis of the morphometries of the sperm head,nucleus,and acrosome using a specific plug-in module created on ImageJ.Acrosomal integrity was related to sperm morphometry as the heads of spermatozoa with a damaged acrosome were significantly smaller than those with a normal acrosome(P<0.001).In the case of spermatozoa with an intact acrosome,those with a damaged plasma membrane had a larger sperm head and acrosome than spermatozoa with an intact plasma membrane(P<0.001).No significant differences in the sperm head size were observed between sperm subpopulations without an acrosome or in the nuclear sperm morphometry of the different subpopulations.There was a positive correlation between the sperm motility values of the samples and the morphometric parameters for in tact spermatozoa.These correlations were particularly strong for the morphometric parameters of the sperm acrosome.We conclude that there are clear differences in the sperm morphometry depending on the status of the sperm membrane and acrosome and this should be considered when performing this kind of analysis.展开更多
With the aim of evaluating how changes in the metabolic status in the last month of pregnancy affects reproductive efficiency, forty six Bos indicus multiparous cows (5.5 ± 2.4 parturitions), were used. Measureme...With the aim of evaluating how changes in the metabolic status in the last month of pregnancy affects reproductive efficiency, forty six Bos indicus multiparous cows (5.5 ± 2.4 parturitions), were used. Measurements of body weight (BW), body condition score (BCS) and dorsal back fat (BF) were taken in the last month of pregnancy, postpartum period previous to synchronization (average 50 d) and at breeding by natural mating following synchronization with a progestin (average 70 d). Average postpartum days to resumption to ovarian activity were 79.96 ± 16.5 d, and average postpartum days to conception was 88.5 ± 14 d. Days postpartum to resumption of the ovarian activity was positively correlated (0.51, P 0.01) with days postpartum to conception, also, days postpartum to conception was positively correlated with prepartum back fat (14 d before parturition) (0.44, P 0.05). It was observed that BF at calving which is an objective measurement had a low correlation with other productive variables such as BCS and BW (always less than 0.39). Body fat might be a more reliable indicator of the current metabolic status of the animal particularly in the last month of pregnancy when the indicators of BCS are somehow more difficult to interpret and subjective.展开更多
A schlieren detection algorithm is proposed for the ground-to-air background oriented schlieren(BOS) system to achieve high-speed airplane shock waves visualization. The proposed method consists of three steps. Firstl...A schlieren detection algorithm is proposed for the ground-to-air background oriented schlieren(BOS) system to achieve high-speed airplane shock waves visualization. The proposed method consists of three steps. Firstly, image registration is incorporated for reducing errors caused by the camera motion.Then, the background subtraction dual-model single Gaussian model(BS-DSGM) is proposed to build a precise background model. The BS-DSGM could prevent the background model from being contaminated by the shock waves. Finally, the twodimensional orthogonal discrete wavelet transformation is used to extract schlieren information and averaging schlieren data. Experimental results show our proposed algorithm is able to detect the aircraft in-flight and to extract the schlieren information. The precision of schlieren detection algorithm is 0.96. Three image quality evaluation indices are chosen for quantitative analysis of the shock waves visualization. The white Gaussian noise is added in the frames to validate the robustness of the proposed algorithm.Moreover, we adopt two times and four times down sampling to simulate different imaging distances for revealing how the imaging distance affects the schlieren information in the BOS system.展开更多
Qaidam cattle(Bos taurus) is an important endemic breed in Northwest China, which is mainly distributed in the northwest of Qinghai-Tibet Plateau. It has strong adaptive ability to plateau and swamp conditions, such a...Qaidam cattle(Bos taurus) is an important endemic breed in Northwest China, which is mainly distributed in the northwest of Qinghai-Tibet Plateau. It has strong adaptive ability to plateau and swamp conditions, such as cold tolerance and insect resistance. In this study, the first complete mitochondrial genome of Qaidam cattle was reported. The circular double-stranded genome is 16 340 bp in size, and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a D-loop region. The overall nucleotide composition is 33.4% A, 27.2% T, 26.0% C and 13.4% G, with a total A +T content of60.6%. The gene order and composition are similar to those of other B. taurus breeds. Molecular phylogenetic analysis indicated that Qaidam cattle was split as an independent clade and nested within Asian cattle breeds.展开更多
The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos...The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos gaurus, is categorized as ＂Vulnerable＂ in the IUCN Red List of Threatened Species, 2009. The study has used ENFA （ecological niche factor analysis） to understand the distribution status of Gaur in TATR （Tadoba-Andhari Tiger Reserve）, Central India. TATR was sampled using stratified random sampling strategy. A total of 21 continuous variables were used, categorised under 4 environmental descriptors categories viz. habitat, anthropogenic, topographic and hydrological variables. All the variables were tested for the correlation and one of the variable among strongly correlated （r 〉 0.7） variables was discarded to avoid redundancy. A total of 14 variables were retained. The model resulted in marginality of 0.56 and specialization of 2.608. Presence of Gaur showed the positive association with canopy density classes （〈 30% ＆ 40-60%） and open forest. However, it was negatively associated with elevation, non-forest, riparian forest, scrub and teak forest. The study has delineated the areas where appropriate habitat conditions exist to sustain Gaur populations vital for planning strategies for conservation of this megaherbivore species in tropical forests.展开更多
On January 17,the Chinese Association for International Understanding(CAFIU)and the Association of Bosnia-China Friendship co-hosted the webinar themed New Vision of China-Bosnia and Herzegovina Cooperation and Role o...On January 17,the Chinese Association for International Understanding(CAFIU)and the Association of Bosnia-China Friendship co-hosted the webinar themed New Vision of China-Bosnia and Herzegovina Cooperation and Role of Civil Society.展开更多
基金the project “Genomics for Conservation of Indigenous Cattle Breeds and for Enhancing Milk Yield, Phase-I” [BT/ PR26466/AAQ/1/704/2017], funded by the Department of Biotechnology (DBT ), Indiathe project “Identification of key molecular factors involved in resistance/susceptibility to paratuberculosis infection in indigenous breeds of cows” [BT/PR32758/AAQ/1/760/2019], which was also funded by Department of Biotechnology (DBT ), India。
文摘Background India harbors the world’s largest cattle population,encompassing over 50 distinct Bos indicus breeds.This rich genetic diversity underscores the inadequacy of a single reference genome to fully capture the genomic landscape of Indian cattle.To comprehensively characterize the genomic variation within Bos indicus and,specifically,dairy breeds,we aim to identify non-reference sequences and construct a comprehensive pangenome.Results Five representative genomes of prominent dairy breeds,including Gir,Kankrej,Tharparkar,Sahiwal,and Red Sindhi,were sequenced using 10X Genomics‘linked-read’technology.Assemblies generated from these linked-reads ranged from 2.70 Gb to 2.77 Gb,comparable to the Bos indicus Brahman reference genome.A pangenome of Bos indicus cattle was constructed by comparing the newly assembled genomes with the reference using alignment and graph-based methods,revealing 8 Mb and 17.7 Mb of novel sequence respectively.A confident set of 6,844 Non-reference Unique Insertions(NUIs)spanning 7.57 Mb was identified through both methods,representing the pange-nome of Indian Bos indicus breeds.Comparative analysis with previously published pangenomes unveiled 2.8 Mb(37%)commonality with the Chinese indicine pangenome and only 1%commonality with the Bos taurus pange-nome.Among these,2,312 NUIs encompassing~2 Mb,were commonly found in 98 samples of the 5 breeds and des-ignated as Bos indicus Common Insertions(BICIs)in the population.Furthermore,926 BICIs were identified within 682 protein-coding genes,54 long non-coding RNAs(lncRNA),and 18 pseudogenes.These protein-coding genes were enriched for functions such as chemical synaptic transmission,cell junction organization,cell-cell adhesion,and cell morphogenesis.The protein-coding genes were found in various prominent quantitative trait locus(QTL)regions,suggesting potential roles of BICIs in traits related to milk production,reproduction,exterior,health,meat,and carcass.Notably,63.21%of the bases within the BICIs call set contained interspersed repeats,predominantly Long Inter-spersed Nuclear Elements(LINEs).Additionally,70.28%of BICIs are shared with other domesticated and wild species,highlighting their evolutionary significance.Conclusions This is the first report unveiling a robust set of NUIs defining the pangenome of Bos indicus breeds of India.The analyses contribute valuable insights into the genomic landscape of desi cattle breeds.
文摘为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)技术,检测了甘南牦牛FBXO32基因突变位点及基因型,分析了基因型与甘南牦牛胴体及肉质性状的相关性。结果表明,从甘南牦牛FBXO32基因检测到7个SNP位点,分别是位于5′UTR区的SNP1(g.267A>C)、外显子1区的SNP2(g.326G>T)、外显子8区的SNP3(g.31231G>C),以及3′UTR区的SNP4(g.31352G>A)、SNP5(g.31424C>T)、SNP6(g.31503A>C)和SNP7(g.31504A>G)。其中,SNP1、SNP4、SNP5、SNP6与肌肉嫩度显著相关(P<0.05),SNP3、SNP6、SNP7与失水率显著相关(P<0.05),SNP1、SNP2、SNP3、SNP4、SNP5、SNP7与眼肌面积显著相关(P<0.05),SNP2、SNP5、SNP6与胴体重显著相关(P<0.05)。7个SNP位点构建的9种有效单倍型组合中,单倍型组合H2H2的个体肌肉嫩度小、熟肉率高,单倍型组合H1H2的个体熟肉率高、眼肌面积大、失水率低。FBXO32基因突变位点可作为甘南牦牛胴体及肉质性状潜在分子遗传标记,研究结果丰富了甘南牦牛经济性状分子遗传理论基础。
基金the National Natural Science Foundation of China(nos.22371084,21072071,21472060 and 21772058)the 111 Project B17019,self-determined research funds of CCNU from the colleges'basic research and operation of MOE(No.CCNU17CG0011,CCNU17KYZHSY07,and CCNU18KYZHSY06)for financial support.
文摘Boscalid is a pesticide with the advantages of broad spectrum bactericidal activity,high efficiency,low toxicity,and no cross-resistance with other fungicides currently available on the market.Herein,we report the synthesis of 40-chloro-2-nitrobiphenyl,a key intermediate of Boscalid using a palladium-catalyzed Suzuki-Miyaura cross-coupling employing the 2-aryl-substituted indenyl phosphine ligand.40-Chloro-2-nitrobiphenyl was prepared in 94%yield on a 100 g scale.This method allows for the industrial production of alimide and active substances bearing a biphenyl moiety.
基金Supported by National Natural Science Foundation of China(30960256,31060314)Project of Yunnan Education Department(V09Y0202)~~
文摘[Objective]The paper was to provide reference for further study and development of gayal（Bos frontalis）.[Method]According to the research status at home and abroad,the biological characteristics and rumen microorganisms of gayal in Yunnan Province was studied.[Result]Gayal in Yunnan had typical body form and very good meat production performance,its muscle fiber diameter was significantly less than other kinds of cattle;the water holding ratio,muscle tenderness and muscle succulency were significantly higher than others;its amount,shape and structure of chromosome were different from yellow cattle（Bos taurus）and wild cattle（Bos gaurus）,and the amounts of those chromosomes（2n）were 58,60 and 56,respectively.It could create hybrid with yellow cattle;the gayal＇s special diet was bamboo,its in vitro dry matter digestibility（IVDMD）on various crude forage was significantly higher than yellow cattle in Yunnan;the viable bacteria and cellulolytic bacteria in rumen were 4.51×109 and 1.63×109 CFU/ml,which was significantly higher than yellow cattle in Yunnan,its dominant bacteria in rumen mainly was cellulolytic bacteria.[Conclusion]Gayal not only had high academic value,but also had a great development value.
文摘[目的]本文旨在研究超高压协同温度处理对过敏原Bos d 6抗原性和二级结构的影响。[方法]采用间接竞争ELISA和圆二色光谱方法,研究在25、40和55℃条件下,100、200、300、400、500和600 MPa压力分别处理10 min对过敏原Bos d 6抗原性及二级结构的影响,并分析过敏原抗原性与其二级结构中α-螺旋的相关性。[结果]超高压处理能有效降低过敏原Bos d 6的抗原性及其二级结构中α-螺旋含量;随着处理温度的增加,过敏原抗原性和α-螺旋含量的降低幅度明显;超高压协同温度处理后过敏原Bos d 6抗原性与α-螺旋含量呈明显的正相关性。[结论]超高压协同温度处理技术可以通过改变过敏原Bos d 6蛋白质的空间构象来达到降低其抗原性的目的。
基金This work was supported by the Spanish Ministry of Economy and Finance(MINECO)(grant AGL2017-85030-R)the European Territorial Cooperation Operational Program-Spain,France,and Andorra Area 2014-20(Program DietaPYR2 EFA144/16)and the DGA-FSE(grant A07_17R).We would like to acknowledge the use of the Research Support Service-SAI,University of Zaragoza.
文摘To date,sperm morphometric studies have assessed whole sperm populations without considering sperm function.The aim of this study was to evaluate the relati on ship of sperm membrane and acrosomal integrity with sperm morphometry in liquid semen samples collected from bulls.To this end,sperm morphometry was performed on cryopreserved semen samples from 16 bulls by a combination of fluorescent dyes,including Hoechst 33343,carboxyfluorescein diacetate,and propidium iodide.This allowed discrimination of different subpopulations on the basis of sperm membrane and acrosomal integrity and analysis of the morphometries of the sperm head,nucleus,and acrosome using a specific plug-in module created on ImageJ.Acrosomal integrity was related to sperm morphometry as the heads of spermatozoa with a damaged acrosome were significantly smaller than those with a normal acrosome(P<0.001).In the case of spermatozoa with an intact acrosome,those with a damaged plasma membrane had a larger sperm head and acrosome than spermatozoa with an intact plasma membrane(P<0.001).No significant differences in the sperm head size were observed between sperm subpopulations without an acrosome or in the nuclear sperm morphometry of the different subpopulations.There was a positive correlation between the sperm motility values of the samples and the morphometric parameters for in tact spermatozoa.These correlations were particularly strong for the morphometric parameters of the sperm acrosome.We conclude that there are clear differences in the sperm morphometry depending on the status of the sperm membrane and acrosome and this should be considered when performing this kind of analysis.
文摘With the aim of evaluating how changes in the metabolic status in the last month of pregnancy affects reproductive efficiency, forty six Bos indicus multiparous cows (5.5 ± 2.4 parturitions), were used. Measurements of body weight (BW), body condition score (BCS) and dorsal back fat (BF) were taken in the last month of pregnancy, postpartum period previous to synchronization (average 50 d) and at breeding by natural mating following synchronization with a progestin (average 70 d). Average postpartum days to resumption to ovarian activity were 79.96 ± 16.5 d, and average postpartum days to conception was 88.5 ± 14 d. Days postpartum to resumption of the ovarian activity was positively correlated (0.51, P 0.01) with days postpartum to conception, also, days postpartum to conception was positively correlated with prepartum back fat (14 d before parturition) (0.44, P 0.05). It was observed that BF at calving which is an objective measurement had a low correlation with other productive variables such as BCS and BW (always less than 0.39). Body fat might be a more reliable indicator of the current metabolic status of the animal particularly in the last month of pregnancy when the indicators of BCS are somehow more difficult to interpret and subjective.
文摘A schlieren detection algorithm is proposed for the ground-to-air background oriented schlieren(BOS) system to achieve high-speed airplane shock waves visualization. The proposed method consists of three steps. Firstly, image registration is incorporated for reducing errors caused by the camera motion.Then, the background subtraction dual-model single Gaussian model(BS-DSGM) is proposed to build a precise background model. The BS-DSGM could prevent the background model from being contaminated by the shock waves. Finally, the twodimensional orthogonal discrete wavelet transformation is used to extract schlieren information and averaging schlieren data. Experimental results show our proposed algorithm is able to detect the aircraft in-flight and to extract the schlieren information. The precision of schlieren detection algorithm is 0.96. Three image quality evaluation indices are chosen for quantitative analysis of the shock waves visualization. The white Gaussian noise is added in the frames to validate the robustness of the proposed algorithm.Moreover, we adopt two times and four times down sampling to simulate different imaging distances for revealing how the imaging distance affects the schlieren information in the BOS system.
基金Supported by China Agriculture Research System(CARS-37)Science and Technology Support Projects in Gansu Province(1504NKCA052)+1 种基金Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2014-LIHPS-01)Central Publicinterest Scientific Institution Basal Research Fund(1610322016006)
文摘Qaidam cattle(Bos taurus) is an important endemic breed in Northwest China, which is mainly distributed in the northwest of Qinghai-Tibet Plateau. It has strong adaptive ability to plateau and swamp conditions, such as cold tolerance and insect resistance. In this study, the first complete mitochondrial genome of Qaidam cattle was reported. The circular double-stranded genome is 16 340 bp in size, and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a D-loop region. The overall nucleotide composition is 33.4% A, 27.2% T, 26.0% C and 13.4% G, with a total A +T content of60.6%. The gene order and composition are similar to those of other B. taurus breeds. Molecular phylogenetic analysis indicated that Qaidam cattle was split as an independent clade and nested within Asian cattle breeds.
文摘The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos gaurus, is categorized as ＂Vulnerable＂ in the IUCN Red List of Threatened Species, 2009. The study has used ENFA （ecological niche factor analysis） to understand the distribution status of Gaur in TATR （Tadoba-Andhari Tiger Reserve）, Central India. TATR was sampled using stratified random sampling strategy. A total of 21 continuous variables were used, categorised under 4 environmental descriptors categories viz. habitat, anthropogenic, topographic and hydrological variables. All the variables were tested for the correlation and one of the variable among strongly correlated （r 〉 0.7） variables was discarded to avoid redundancy. A total of 14 variables were retained. The model resulted in marginality of 0.56 and specialization of 2.608. Presence of Gaur showed the positive association with canopy density classes （〈 30% ＆ 40-60%） and open forest. However, it was negatively associated with elevation, non-forest, riparian forest, scrub and teak forest. The study has delineated the areas where appropriate habitat conditions exist to sustain Gaur populations vital for planning strategies for conservation of this megaherbivore species in tropical forests.
文摘On January 17,the Chinese Association for International Understanding(CAFIU)and the Association of Bosnia-China Friendship co-hosted the webinar themed New Vision of China-Bosnia and Herzegovina Cooperation and Role of Civil Society.
