Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with ...Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxol, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation.展开更多
To improve the training speed of support vector machine （SVM）, a method called improved center distance ratio method （ICDRM） with determining thresholds automatically is presented here without reduce the identific...To improve the training speed of support vector machine （SVM）, a method called improved center distance ratio method （ICDRM） with determining thresholds automatically is presented here without reduce the identification rate. In this method border vectors are chosen from the given samples by comparing sample vectors with center distance ratio in advance. The number of training samples is reduced greatly and the training speed is improved. This method is used to the identification for license plate characters. Experimental resuhs show that the improved SVM method-ICDRM does well at identification rate and training speed.展开更多
文摘Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxol, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation.
基金Sponsored by the National Natural Science Foundation of China(60472110)
文摘To improve the training speed of support vector machine （SVM）, a method called improved center distance ratio method （ICDRM） with determining thresholds automatically is presented here without reduce the identification rate. In this method border vectors are chosen from the given samples by comparing sample vectors with center distance ratio in advance. The number of training samples is reduced greatly and the training speed is improved. This method is used to the identification for license plate characters. Experimental resuhs show that the improved SVM method-ICDRM does well at identification rate and training speed.
