Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanis...Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides(ADBP)on HUA-induced kidney damage.In the present study,we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents.In vitro results found that ADBP protected uric acid(UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense.In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice,evidenced by a remarkable decrease in serum UA,creatinine and blood urea nitrogen,improving kidney UA excretion,antioxidant defense and histological kidney deterioration.Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction.Intriguingly,ADBP restored the gut microbiome homeostasis in CKD mice,especially with respect to the elevated helpful microbial abundance,and the decreased harmful bacterial abundance.This study demonstrated that ADBP displayed great nephroprotective effects,and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.展开更多
[Objectives]To explore the impact of bone collagen peptide(BCP)on patients with knee osteoarthritis(KOA).[Methods]A total of 100 patients diagnosed with KOA were admitted to the study and randomly assigned to either a...[Objectives]To explore the impact of bone collagen peptide(BCP)on patients with knee osteoarthritis(KOA).[Methods]A total of 100 patients diagnosed with KOA were admitted to the study and randomly assigned to either a control group or a treatment group,with each group comprising 50 participants.The control group received health education along with standard daily treatment protocols.In contrast,the treatment group was administered an additional dosage of 20 g of BCP twice daily,in conjunction with the treatment regimen provided to the control group.Both groups received continuous treatment for 3 months.The WOMAC scores and the WHOQOL-BREF scores of the participants in both groups were assessed both prior to and following treatment.[Results]Following treatment,the WOMAC scores of patients in the treatment group demonstrated a significant improvement compared to those in the control group(13.39±2.19 vs.15.46±1.30,P<0.05).Additionally,the WHOQOL-BREF scores for patients in both groups showed improvement,with the treatment group exhibiting superior WHOQOL-BREF scores relative to the control group(P<0.05).[Conclusions]For patients diagnosed with KOA,the supplementation of BCP alongside conventional treatment has been shown to significantly enhance knee joint function and improve the overall quality of life for these individuals.展开更多
This present work aims to functionalize poly（amidoamine） （PAMAM） dendrimers with various reported adhesive peptides, including Arg-Gly-Asp （RGD）, Tyr-lle-Gly-Ser-Arg （YIGSR）, and Ile-Lys-Val-Ala-Val （IKVAV） ...This present work aims to functionalize poly（amidoamine） （PAMAM） dendrimers with various reported adhesive peptides, including Arg-Gly-Asp （RGD）, Tyr-lle-Gly-Ser-Arg （YIGSR）, and Ile-Lys-Val-Ala-Val （IKVAV） for enhancing cell responses. The RGD, YIGSR, or IKVAV functionalized PAMAM coated substrate could promote cell adhesion of bone marrow mesenchymal stem cells （BMSCs） within 1 h after incubation. The neurite differentiation and proliferation of pheochromocytoma （PC12） cells were also significantly enhanced after culturing on the peptide functionalized PAMAM dendrimers for two and foul days. This peptide functionalized PAMAM dendrimers are considered as the potential candidates for various tissue engineering applications.展开更多
The complex pathogenesis of osteoporosis includes excessive bone resorption,insufficient bone formation and inadequate vascularization,a combination which is difficult to completely address with conventional therapies...The complex pathogenesis of osteoporosis includes excessive bone resorption,insufficient bone formation and inadequate vascularization,a combination which is difficult to completely address with conventional therapies.Engineered exosomes carrying curative molecules show promise as alternative osteoporosis therapies,but depend on specifically-functionalized vesicles and appropriate engineering strategies.Here,we developed an exosome delivery system based on exosomes secreted by mesenchymal stem cells(MSCs)derived from human induced pluripotent stem cells(iPSCs).The engineered exosomes BT-Exo-siShn3,took advantage of the intrinsic anti-osteoporosis function of these special MSC-derived exosomes and collaborated with the loaded siRNA of the Shn3 gene to enhance the therapeutic effects.Modification of a bone-targeting peptide endowed the BT-Exo-siShn3 an ability to deliver siRNA to osteoblasts specifically.Silencing of the osteoblastic Shn3 gene enhanced osteogenic differentiation,decreased autologous RANKL expression and thereby inhibited osteoclast formation.Furthermore,Shn3 gene silencing increased production of SLIT3 and consequently facilitated vascularization,especially formation of type H vessels.Our study demonstrated that BT-Exo-siShn3 could serve as a promising therapy to kill three birds with one stone and implement comprehensive anti-osteoporosis effects.展开更多
基金financially supported by Shenzhen Agricultural Development Special Fund(Fishery)Agricultural High-Tech Project([2021]735)the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022)Youth Science Foundation Project(32101936)。
文摘Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides(ADBP)on HUA-induced kidney damage.In the present study,we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents.In vitro results found that ADBP protected uric acid(UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense.In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice,evidenced by a remarkable decrease in serum UA,creatinine and blood urea nitrogen,improving kidney UA excretion,antioxidant defense and histological kidney deterioration.Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction.Intriguingly,ADBP restored the gut microbiome homeostasis in CKD mice,especially with respect to the elevated helpful microbial abundance,and the decreased harmful bacterial abundance.This study demonstrated that ADBP displayed great nephroprotective effects,and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.
基金Supported by the Horizontal Research Program"Observational Study on the Therapeutic Effects of Collagen Protein Peptides in Knee Osteoarthritis"(KY202208-1-119).
文摘[Objectives]To explore the impact of bone collagen peptide(BCP)on patients with knee osteoarthritis(KOA).[Methods]A total of 100 patients diagnosed with KOA were admitted to the study and randomly assigned to either a control group or a treatment group,with each group comprising 50 participants.The control group received health education along with standard daily treatment protocols.In contrast,the treatment group was administered an additional dosage of 20 g of BCP twice daily,in conjunction with the treatment regimen provided to the control group.Both groups received continuous treatment for 3 months.The WOMAC scores and the WHOQOL-BREF scores of the participants in both groups were assessed both prior to and following treatment.[Results]Following treatment,the WOMAC scores of patients in the treatment group demonstrated a significant improvement compared to those in the control group(13.39±2.19 vs.15.46±1.30,P<0.05).Additionally,the WHOQOL-BREF scores for patients in both groups showed improvement,with the treatment group exhibiting superior WHOQOL-BREF scores relative to the control group(P<0.05).[Conclusions]For patients diagnosed with KOA,the supplementation of BCP alongside conventional treatment has been shown to significantly enhance knee joint function and improve the overall quality of life for these individuals.
基金financially supported by the NSF-ECCS 1509760NSF EPSCoR RII Track 1 cooperative agreement awarded to the University of South Carolina (NSF EPSCoR Cooperative Agreement No. EPS-0903795)
文摘This present work aims to functionalize poly（amidoamine） （PAMAM） dendrimers with various reported adhesive peptides, including Arg-Gly-Asp （RGD）, Tyr-lle-Gly-Ser-Arg （YIGSR）, and Ile-Lys-Val-Ala-Val （IKVAV） for enhancing cell responses. The RGD, YIGSR, or IKVAV functionalized PAMAM coated substrate could promote cell adhesion of bone marrow mesenchymal stem cells （BMSCs） within 1 h after incubation. The neurite differentiation and proliferation of pheochromocytoma （PC12） cells were also significantly enhanced after culturing on the peptide functionalized PAMAM dendrimers for two and foul days. This peptide functionalized PAMAM dendrimers are considered as the potential candidates for various tissue engineering applications.
基金supported by the National Natural Science Foundation of China(Grant No:81874026 and 82070911).
文摘The complex pathogenesis of osteoporosis includes excessive bone resorption,insufficient bone formation and inadequate vascularization,a combination which is difficult to completely address with conventional therapies.Engineered exosomes carrying curative molecules show promise as alternative osteoporosis therapies,but depend on specifically-functionalized vesicles and appropriate engineering strategies.Here,we developed an exosome delivery system based on exosomes secreted by mesenchymal stem cells(MSCs)derived from human induced pluripotent stem cells(iPSCs).The engineered exosomes BT-Exo-siShn3,took advantage of the intrinsic anti-osteoporosis function of these special MSC-derived exosomes and collaborated with the loaded siRNA of the Shn3 gene to enhance the therapeutic effects.Modification of a bone-targeting peptide endowed the BT-Exo-siShn3 an ability to deliver siRNA to osteoblasts specifically.Silencing of the osteoblastic Shn3 gene enhanced osteogenic differentiation,decreased autologous RANKL expression and thereby inhibited osteoclast formation.Furthermore,Shn3 gene silencing increased production of SLIT3 and consequently facilitated vascularization,especially formation of type H vessels.Our study demonstrated that BT-Exo-siShn3 could serve as a promising therapy to kill three birds with one stone and implement comprehensive anti-osteoporosis effects.
