Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in man...Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in man...Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
The published article titled“MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.523–536.DOI:10.3727/096504016X14...The published article titled“MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.523–536.DOI:10.3727/096504016X14756282819385 URL:https://www.techscience.com/or/v25n4/56833 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed ...Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in man...Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
The published article titled“Knockdown of Rap1b Enhances Apoptosis and Autophagy in Gastric Cancer Cells via the PI3K/Akt/mTOR Pathway”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.287–293.DOI:10.37...The published article titled“Knockdown of Rap1b Enhances Apoptosis and Autophagy in Gastric Cancer Cells via the PI3K/Akt/mTOR Pathway”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.287–293.DOI:10.3727/096504016X14648701447779 URL:https://www.techscience.com/or/v24n5/56977 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels bas...Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels based on its slightly higher molecular weight(MW)than that of endogenous EPO using western blotting(WB).However,a type of variant erythropoietin(VAR-EPO)encoded by the EPO c.577del variant has a similar MW to rEPO,and these 2 molecules cannot be distinguished using conventional analytical methods.A fit-for-purpose method needs to be developed immediately.Methods In this study,we introduced a reverse–normal immunopurification technique for sample pretreatment to remove VAR-EPO from samples to eliminate its interference with rEPO detection.Firstly,a rabbit monoclonal antibody(mAb)that can specifically recognize trace amounts of VAR-EPO with high affinity was generated.Then,using this antibody to enrich VAR-EPO,we developed reverse–normal immunopurification coupled with WB on the purpose of analyzing rEPO in urine and serum samples.Next,the method was fully validated and evaluated using blank samples,spiked samples and rEPO excreted samples.Finally,the identification criteria of rEPO was established.Results A specific anti-VAR mAb with high affinity was developed.Using it,we developed the doping analytical method for rEPO.Our method effectively detects and removes VAR-EPO,enabling accurate rEPO detection.Conclusion A method has already been applied for rEPO confirmation in routine doping analyses.展开更多
The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on...The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on exploring valuable nutrients derived from Cannabis sativa L.(hemp)seeds,particularly hemp seed proteins.Therefore,this study aimed to investigate the release of anti-inflammatory peptides from Lactobacillus paraplantarum-fermented hemp seed proteins.To confirm the complete hydrolysis of hemp seed proteins during the fermentation process,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was employed.Further,the isolation and purification of peptides were achieved through ultrafiltration.The identity of peptides was nextly established using ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MS).The results revealed a total of 39 identified peptides in fermented hemp seeds,with 9 peptides selected based on their relative quantity.Notably,AAELIGVP(P1),AAVPYPQ(P2),VFPEVAP(P4),DVIGVPLG(P6),and PVPKVL(P9)demonstrated strong anti-inflammatory abilities in lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells.Molecular docking was used to understand the potential anti-inflammatory mechanism of these 5 peptides,and in silico results indicated that P1,P2,P4,P6,and P9 could bind to the active sites of toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),and inhibitor of NF-κB kinase(IKK)with higher binding energies.Overall,these findings indicate that hemp seeds have potential to be a source of bioactive peptides for functional foods with anti-inflammatory properties.展开更多
Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X1...Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X14666990347590 URL:https://www.techscience.com/or/v24n5/56980.Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Corrigendum:Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway https://doi.org/10.4103/NRR.NRR-D-25-00562 In the article titled“Epalrestat protect...Corrigendum:Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway https://doi.org/10.4103/NRR.NRR-D-25-00562 In the article titled“Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway,”published on pages 345-351 in Issue 2,Volume 11 of Neural Regeneration Research(Li et al.,2016),the Western blot bands in Figure 2A are incorrect.展开更多
Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devi...Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies.However,viruses are complex to propagate and quantify.Existing methodologies for viral quantification tend to be expensive and time-consuming.Here,we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter.Using a microplate reader,we measured viral protein levels and we validated our findings through comparison by western blot analysis of viral coat protein,the most common approach to quantify viral titer.Our proposed methodology provides a practical and accessible approach to studying virus-host interactions and could contribute to enhancing our understanding of plant virology.展开更多
文摘Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘The published article titled“MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.523–536.DOI:10.3727/096504016X14756282819385 URL:https://www.techscience.com/or/v25n4/56833 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘The published article titled“Knockdown of Rap1b Enhances Apoptosis and Autophagy in Gastric Cancer Cells via the PI3K/Akt/mTOR Pathway”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.287–293.DOI:10.3727/096504016X14648701447779 URL:https://www.techscience.com/or/v24n5/56977 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
基金WADA and Beijing Sport University for funding under grant numbers 22B06XZ and 2022YB011
文摘Background Recombinant erythropoietin(rEPO)is commonly used in therapy but may be abused in sports to enhance endurance.In doping analysis,rEPO can be detected in human urine or blood samples at picogram(pg)levels based on its slightly higher molecular weight(MW)than that of endogenous EPO using western blotting(WB).However,a type of variant erythropoietin(VAR-EPO)encoded by the EPO c.577del variant has a similar MW to rEPO,and these 2 molecules cannot be distinguished using conventional analytical methods.A fit-for-purpose method needs to be developed immediately.Methods In this study,we introduced a reverse–normal immunopurification technique for sample pretreatment to remove VAR-EPO from samples to eliminate its interference with rEPO detection.Firstly,a rabbit monoclonal antibody(mAb)that can specifically recognize trace amounts of VAR-EPO with high affinity was generated.Then,using this antibody to enrich VAR-EPO,we developed reverse–normal immunopurification coupled with WB on the purpose of analyzing rEPO in urine and serum samples.Next,the method was fully validated and evaluated using blank samples,spiked samples and rEPO excreted samples.Finally,the identification criteria of rEPO was established.Results A specific anti-VAR mAb with high affinity was developed.Using it,we developed the doping analytical method for rEPO.Our method effectively detects and removes VAR-EPO,enabling accurate rEPO detection.Conclusion A method has already been applied for rEPO confirmation in routine doping analyses.
基金the 4^(th) Brain Korea(BK)21 Plus Project(4299990913942)financed by the Korean Government,Republic of Korea.
文摘The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on exploring valuable nutrients derived from Cannabis sativa L.(hemp)seeds,particularly hemp seed proteins.Therefore,this study aimed to investigate the release of anti-inflammatory peptides from Lactobacillus paraplantarum-fermented hemp seed proteins.To confirm the complete hydrolysis of hemp seed proteins during the fermentation process,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was employed.Further,the isolation and purification of peptides were achieved through ultrafiltration.The identity of peptides was nextly established using ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MS).The results revealed a total of 39 identified peptides in fermented hemp seeds,with 9 peptides selected based on their relative quantity.Notably,AAELIGVP(P1),AAVPYPQ(P2),VFPEVAP(P4),DVIGVPLG(P6),and PVPKVL(P9)demonstrated strong anti-inflammatory abilities in lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells.Molecular docking was used to understand the potential anti-inflammatory mechanism of these 5 peptides,and in silico results indicated that P1,P2,P4,P6,and P9 could bind to the active sites of toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),and inhibitor of NF-κB kinase(IKK)with higher binding energies.Overall,these findings indicate that hemp seeds have potential to be a source of bioactive peptides for functional foods with anti-inflammatory properties.
文摘Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X14666990347590 URL:https://www.techscience.com/or/v24n5/56980.Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘Corrigendum:Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway https://doi.org/10.4103/NRR.NRR-D-25-00562 In the article titled“Epalrestat protects against diabetic peripheral neuropathy by alleviating oxidative stress and inhibiting polyol pathway,”published on pages 345-351 in Issue 2,Volume 11 of Neural Regeneration Research(Li et al.,2016),the Western blot bands in Figure 2A are incorrect.
基金Funding from Natural Sciences and Engineering Research Council of Canada award number RGPIN/4002-2020.
文摘Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies.However,viruses are complex to propagate and quantify.Existing methodologies for viral quantification tend to be expensive and time-consuming.Here,we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter.Using a microplate reader,we measured viral protein levels and we validated our findings through comparison by western blot analysis of viral coat protein,the most common approach to quantify viral titer.Our proposed methodology provides a practical and accessible approach to studying virus-host interactions and could contribute to enhancing our understanding of plant virology.
