Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut...Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.展开更多
[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore wer...[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore were isolated from soil in different places. Through screening and rescreening, the bacteria with higher antibacterial activity were conducted observation about thallus shapes and colony characters, a series of physiological and biochemical tests were performed. [Result] Rescreening results indicated that the strains including ZG-10, ZG-19, ZG-59, ZG-72 and ZG-31 had significant antibacterial activity, which had very high research value and good application prospect for biocontrol on spot blotch disease in pakchoi; the strain ZG-10 was identified to be Bacillus subtilis. [ Conclusion] The strain ZG-10 had biocontrol potential and good development prospect. This research laid certain basis for subsequent research and strated a new way for the application of antagonistic strain and proteinum polypeptide in agdculture.展开更多
Citrus leaf blotch virus （CLBV） is a member of the genus Citrivirus, in the family Betaflexiviridae. It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regi...Citrus leaf blotch virus （CLBV） is a member of the genus Citrivirus, in the family Betaflexiviridae. It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study. The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan （CLBV-LH）, Yura Wase in Zhejiang （CLBV-YL）, Bingtangcheng in Hunan （CLBV-BT）, Fengjie 72-1 in Chongqing （CLBV- F J）, respectively. While they all represented 8 747 nucleotides in monopartite size, excluding the poly（A） tail, each of the isolates coded three open reading frames （ORFs）. Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes. A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation. This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.展开更多
In this study, hyperspectral images were used to detect a fungal disease in apple leaves called Marssonina blotch(AMB). Estimation models were built to classify healthy, asymptomatic and symptomatic classes using part...In this study, hyperspectral images were used to detect a fungal disease in apple leaves called Marssonina blotch(AMB). Estimation models were built to classify healthy, asymptomatic and symptomatic classes using partial least squares regression(PLSR), principal component analysis(PCA), and linear discriminant analysis(LDA) multivariate methods. In general, the LDA estimation model performed the best among the three models in detecting AMB asymptomatic pixels, while all the models were able to detect the symptomatic class. LDA correctly classified asymptomatic pixels and LDA model predicted them with an accuracy of 88.0%. An accuracy of 91.4% was achieved as the total classification accuracy. The results from this work indicate the potential of using the LDA estimation model to identify asymptomatic pixels on leaves infected by AMB.展开更多
Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F...Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F1 hybrid was obtained by crossing the Silihong(Slh) cultivar with Arachis duranensis(ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization(Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55;2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats;3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.展开更多
Purple blotch disease of Allium spp. crops caused by Alternaria porri has remained a major concern in agriculture for both farmers and research fraternity as it severely damages the crops and drastically reduces the y...Purple blotch disease of Allium spp. crops caused by Alternaria porri has remained a major concern in agriculture for both farmers and research fraternity as it severely damages the crops and drastically reduces the yield. The symptoms appear after 1–4 days of infection and bulb rot begin, and eventually turn into dark reddish-purple and then brownish/black lesions. Many factors like season, time of sowing, humidity and temperature, stage of crop, and plant architecture have a huge impact on the progression of purple blotch disease. Many genic markers based on amplification of an Alta1 gene sequence have been designed for identification and differentiation of different Alternaria species groups. Among the most commonly used fungicides, mancozeb, tebuconazole, difenaconazole and azoxystrobin were found to be the ideal for the management of purple blotch disease and increased garlic yield. Many biological approaches such as plant extracts and bio-control agents were found partially effective for controlling the disease. A report on QTL mapping for purple blotch resistance discovered that purple blotch resistance is controlled by a single dominant gene ApR1. To completely understand the purple blotch disease resistance for crop improvement, a study is required at transcriptome level for hunting purple blotch resistant genes by gene annotation and mining. Genetic engineering and genome editing are other approaches that can be done for engineering disease resistance in Allium crops for genetic improvement.展开更多
In the present study, agar diffusion test and field trials were carried out using a mushroom （Pleurotus ostreatus） culti- var Shouyanping as the experimental material to determine the antibacterial activity of refin...In the present study, agar diffusion test and field trials were carried out using a mushroom （Pleurotus ostreatus） culti- var Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasfi and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom, but also greatly promoted mushroom mycelial growth. Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.展开更多
Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In...Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In the wheat-peanut interplanting regional experiment conducted in Henan Province during 2012-2013, the average pod and seed yield of Shanghua 511 were 5906.19 and 4149.3 kg/hm2, respectively. In the wheat-peanut interplanting production experiment conducted in Henan Province in 2013, the average pod and seed yield of Shanghua 511 were 6036.45 and 4309.50 kg/hm2, respectively. In the kernels of Shanghua 511, the contents of crude fat, oleic acid and linoleic acid were 56.62%, 44.9% and 33.4% respectively, and the oleic/linoleic acid ratio was 1.34. This variety had high resistance to web blotch and medium resistance to leaf spot, viral diseases, root rot and collar rot. The 100-pod weight, 100-kernel weight, shelling percentage and growth period of Shanghua 511 were 270.6, 110.9 g, 70.6% and 120 d, respectively. Shanghua 511 passed the approval of Henan Provincial Crop Variety Approval Committee in August, 2015, and is suitable for spring sowing and wheat interplanting in the surrounding area of Henan Province and the area to the north of Huaihe River.展开更多
This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen....This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight different onion growing areas were characterized in terms of cultural and morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish, light grey to whitish, deep greenish white, light grey and dirty white to greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate, raised and flat type colony elevation. Colony textures were cottony, fluffy and velvety with entire, undulate and filiform type colony margin. Among the culture media, V-7 juice agar found to be the most suitable culture media for mycclial growth of S. vesicarium. The sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato dextrose agar （PDA） media, this media exhibited the highest sporulation （87.76-169.0/mm^2） of S. vesicariumin comparison with other media. The minimum days （28 d to 31 d） for conidial production were observed on V-7 juice agar medium. The length of conidia varied from 14.6 μm to 30.6 μm. The maximum mean length of conidia was 29.97 μm found in isolate DSSA, while the minimum mean length 17.36 μm was found in isolate MSMM 02. The breadth of conidia ranged from 4.7 μm to 15.7μm. The maximum mean breadth of conidia was 12.55 μm found in the isolate DSSA, while the minimum mean breadth 9.760 μm was found in the isolate CCKH 02. The horizontal septation varied from l to 3 and the longitudinal septation varied from 0 to 4.展开更多
An experiment was carried out to study the outcome of environmental factors (Temperature, Relative Humidity, Rainfall) and<span style="font-family:;" "=""> </span><span style=&q...An experiment was carried out to study the outcome of environmental factors (Temperature, Relative Humidity, Rainfall) and<span style="font-family:;" "=""> </span><span style="font-family:Verdana;">management of the purple blotch disease of onion using five treatments (Rovral + Ridoil gold with 3,4,5,6 sprays and control). The Taherpuri variety was used during the 2015-2016 cropping seasons at BARI, Gazipur, Bangladesh.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">In the experiment, the purple blotch disease surfaced at the end of January</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">2016 and was severe in late February to March (PDI 76.12%) due to temperature and RH above 24</span><span style="font-family:Verdana;">°C and 95%, respectively.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">As for the chemical control, the PDI for the treatments ranged from 50.66% to 83.33%.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">Significantly higher stem height was recorded by T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (68.06 cm), where six sprays of Rovral + Ridomil were applied. The minimum stem height was recorded in T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (59.23 cm). The maximum stem number per hill was recorded in T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (2.30) numerically but differed significantly with T</span><sub><span style="font-family:Verdana;">5</span></sub></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">(2.00), where fungicides were not applied. The maximum lesion area was measured in T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (25.26 mm</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">). Regarding PDI (Percent disease index), there was a significant difference among the treatments. The PDI value on purple blotch of onion ranged from 34.00 to 83.33. The lowest PDI was assessed in T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (34.00). Statistical analysis revealed that a significant decrease of PDI on purple blotch was recorded under treatments of T</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;">, T</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">, T</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, and T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (39.20</span></span><span style="font-family:Verdana;">%</span><span style="font-family:Verdana;">, 45.60</span><span style="font-family:Verdana;">%</span><span style="font-family:Verdana;">, 49.59</span><span style="font-family:Verdana;">%</span><span style="font-family:;" "=""><span style="font-family:Verdana;">, and 59.19%) on controlling the disease and giving the optimum yield but all of them significantly differed with T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (83.33%) where no fungicides were sprayed.</span></span>展开更多
Reactions 13 wheat cultivars to 18 Mycosphaerella graminicola isolates, collected from three distinct localities, were in average different but differences among individual cultivars were small. All cultivars were sus...Reactions 13 wheat cultivars to 18 Mycosphaerella graminicola isolates, collected from three distinct localities, were in average different but differences among individual cultivars were small. All cultivars were susceptible to septoria tritici blotch. Reactions of wheat cultivars to M. graminicola isolates varied from 31% to 41%. Some cultivars had low differences but the other high in reactions to used isolates in average. Variance of individual cultivars to all isolates in reactions of maximal and minimal values fluctuated between 44-80%. More differences were in reactions between individual isolates to single cultivars. Aggressiveness individual isolates to all tested cultivars oscillated in average from 9% to 66% of necrotic leaf area covered by pycnidia. Three isolates had very low, whereas five isolates had high aggressiveness in average to all cultivars. Difference in maximal and minimal aggressiveness of individual isolates to all cultivars was between 13 and 60%. Avirulent reaction was ascertained in the cultivar Rexia to one isolate. Isolates showed wide differences in colored and morphological characteristics. Dissimilarity and similarity was in border shape, cultivation types, surface and coloration of isolate colony also.展开更多
Both grain yield and disease performance are important factors to consider for winter wheat （Triticum aestivum） cultivar selection. However, disease index and yield data are often presented separately, making it dif...Both grain yield and disease performance are important factors to consider for winter wheat （Triticum aestivum） cultivar selection. However, disease index and yield data are often presented separately, making it difficult to compare values across multiple environments. Two-year investigations （2009-2010）, in which eight common wheat lines/genotypes were included to test their susceptibility against wheat LR （leaf rust） Puccinia triticina f. sp. tritici Roberge ex Desm., and the SLB （SeptoriaJStagonospora leaf blotch） complex Septoria tritici Desm. and Stagonospora nodorum, were carried out in the experimental field of ATTC （Agriculture Technology Transfer Centre） of Lushnje （Albania）. The objective of this study was to use a rank-based method to compare cultivars based on yield and disease performance combined across multiple environments. Analysis of variance revealed the presence of an important and significant variability in the experimental materials used to evaluate the susceptibility and the resistance of common wheat lines against SLB and LR. There were high negative correlations between yieldS, tritici （0.6683） and yield--P, recondita f. sp. tritici （0.5261）. The negative effects of two pathogens have shown ＂the parallel＂/similar negative influences on yield trait and there was a high positive correlation （0.7631） between S. tritici and P. recondita f. sp. tritici. According to study results the lines Regina × L-776, IKB-P6 and Bullgar 3 × KB 703 have shown good results of resistance （R） level against S. tritici and P. recondita f. sp. tritici.展开更多
[Objective] The paper was to explore safe fungicides with high efficiency and long persistence against eucalyptus purple blotch. [Method]10%Difenoconazole WG,500 g /L thiophanate-methyl SC and 50% carbendazim WP were ...[Objective] The paper was to explore safe fungicides with high efficiency and long persistence against eucalyptus purple blotch. [Method]10%Difenoconazole WG,500 g /L thiophanate-methyl SC and 50% carbendazim WP were evaluated through experiment in forest,and clear water was used as CK. [Result]Three fungicides all had relatively good control effect against eucalyptus purple blotch. Most eucalyptus trees were able to restore growth and sprout new shoots after continuous three times of administration with the interval of 10 d. 10% Difenoconazole WG possessed the best control effect against eucalyptus purple blotch,with the disease index of 25. 24 and the control effect of 40. 24%; followed by 500 g /L thiophanate-methyl SC 600 times dilution,with the disease index of 27. 09 and the control effect of 35. 87%; 50% carbendazim WP 500 times dilution had the poorest control effect,with the disease index of 32. 21 and the control effect of23. 74%. The disease index of CK was 42. 24. [Conclusion]The above fungicides were safe to eucalyptus,and could be used to control eucalyptus purple blotch.展开更多
瓜类细菌性果斑病(bacterial fruit blotch)是瓜类作物上重要的种传细菌性病害,病原菌为西瓜噬酸菌Acidovorax citrulli。我国是全球西甜瓜的主要生产区。近年来,瓜类细菌性果斑病的频繁发生已严重影响我国西甜瓜产业的健康发展。为明...瓜类细菌性果斑病(bacterial fruit blotch)是瓜类作物上重要的种传细菌性病害,病原菌为西瓜噬酸菌Acidovorax citrulli。我国是全球西甜瓜的主要生产区。近年来,瓜类细菌性果斑病的频繁发生已严重影响我国西甜瓜产业的健康发展。为明确瓜类细菌性果斑病在我国的适生性,根据其在全球的最新分布数据,本研究利用MaxEnt模型结合ArcGIS软件预测了瓜类细菌性果斑病在我国的潜在地理分布。结果表明,MaxEnt模型的平均AUC(area under curve,AUC)值均大于0.9,预测结果的准确性较高。在历史气候条件下,瓜类细菌性果斑病适生区分布广泛,主要包括华中、华南和华东地区,以及部分华北、东北地区,占我国面积的47.36%。影响瓜类细菌性果斑病在我国潜在分布区域的主要气候因子包括最热月份最高温度、月平均昼夜温差、最干月份降水量和最干季平均温度。未来气候情景无论是低环境强迫还是高环境强迫,适生区面积均呈现增长的趋势,预示着随着气候的变化,瓜类细菌性果斑病在我国发生的风险不断增加,因此建议应加强检疫监测和防控,严防其扩散。展开更多
Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from cli...Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from climate change can affect the severity of plant diseases.Therefore,two primary objectives of integrated disease management regarding climate change are identifying cultivars resistant to foliar diseases and understanding disease progression under abiotic stress.In the current study,we assessed the quantitative foliar disease resistance of 17 commercial barley cultivars under both normal and water stress conditions over two growing seasons(from 2020/21 to 2021/22).The findings demonstrated a reduced incidence of foliar fungal diseases(leaf rust,net blotch,and powdery mildew)under severe drought stress relative to standard irrigated field conditions.The barley cultivars(Giza 130,Giza 131,and Giza 133)demonstrated significant differences across all disease resistance indices.In addition,the study aimed to molecularly characterize 17 commercial barley varieties using single-cell DNA testing(SCoT)to identify genetic polymorphism and specific markers for each genotype.Eight SCoT primers were employed to investigate the genetic polymorphism among 17 barley varieties.Furthermore,these cultivars exhibited optimal performance for the majority of agricultural attributes examined,both under normal and water-stressed conditions.展开更多
Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii),possessing significant aesthetic value and playing a crucial role in the species'reproduction and fitness.Despite y...Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii),possessing significant aesthetic value and playing a crucial role in the species'reproduction and fitness.Despite years of research,deciphering the molecular mechanisms underlying blotch formation remains challenging.As is well known,floral pigmentation is frequently associated with the familiar R2R3-MYB transcription factors.The key MYB anthocyanin activators of P.rockii‘Shu Sheng Peng Mo’were previously reported in our preceding study.In this study,we identified and characterized three R2R3-MYBs,PrMYBi1,PrMYBi2,and PrMYBi3,which belong to subgroup 4(SG4)and play repressor roles in anthocyanin biosynthesis.A quantitative real-time PCR(qRT-PCR)assay indicated that the expression of PrMYBi1 and PrMYBi3 gradually increased during flowering development and was substantially up-regulated in non-blotch compared to blotch.Yeast one-hybrid and dualluciferase assays demonstrated that PrMYBi(1e3)directly target the anthocyanin structural genes and repress their transcription.The genetic transformation of tobacco demonstrated that the overexpression of PrMYBi(1e3)decreased anthocyanin accumulation in flowers,with PrMYBi1 serving as the most effective repressor.Our results revealed that SG4 R2R3-MYBs negatively regulate the anthocyanin pathway in P.rockii conservatively,and we provide the definite members.These findings will advance future research to unravel the mystery of blotch pattern formation.展开更多
基金supported by the Key Research Project of the Shennong Laboratory,China(SN01-2022-03)the Henan Provincial Science and Technology R&D Program Joint Fund(Superiority Discipline Cultivation)Project,China(222301420100)+4 种基金the Major Science and Technology Projects of Henan Province,China(221100110300)the China Agriculture Research System of MOF and MARA(CARS-13)the Henan Provincial Agriculture Research System,China(S2012-5)the Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16)the Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093)。
文摘Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.
文摘[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore were isolated from soil in different places. Through screening and rescreening, the bacteria with higher antibacterial activity were conducted observation about thallus shapes and colony characters, a series of physiological and biochemical tests were performed. [Result] Rescreening results indicated that the strains including ZG-10, ZG-19, ZG-59, ZG-72 and ZG-31 had significant antibacterial activity, which had very high research value and good application prospect for biocontrol on spot blotch disease in pakchoi; the strain ZG-10 was identified to be Bacillus subtilis. [ Conclusion] The strain ZG-10 had biocontrol potential and good development prospect. This research laid certain basis for subsequent research and strated a new way for the application of antagonistic strain and proteinum polypeptide in agdculture.
基金supported by the National Natural Science Foundation of China (31501611)the Chongqing Research Program of Basic Research and Frontier Technology, China (cstc2017jcyjB X0016)+2 种基金the Chongqing Science and Technology Commission Project, China (cstc2016shmsztzx80003)the Fundamental Research Funds for the Central Universities, China (XDJK2016B21, SWU116012)the Special Fund for Agro-scientific Research in the Public Interest, China (201203076-01)
文摘Citrus leaf blotch virus （CLBV） is a member of the genus Citrivirus, in the family Betaflexiviridae. It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study. The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan （CLBV-LH）, Yura Wase in Zhejiang （CLBV-YL）, Bingtangcheng in Hunan （CLBV-BT）, Fengjie 72-1 in Chongqing （CLBV- F J）, respectively. While they all represented 8 747 nucleotides in monopartite size, excluding the poly（A） tail, each of the isolates coded three open reading frames （ORFs）. Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes. A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation. This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.
基金supported by the National Academy of Agricultural Science,Rural Development Administration,Republic of Korea.
文摘In this study, hyperspectral images were used to detect a fungal disease in apple leaves called Marssonina blotch(AMB). Estimation models were built to classify healthy, asymptomatic and symptomatic classes using partial least squares regression(PLSR), principal component analysis(PCA), and linear discriminant analysis(LDA) multivariate methods. In general, the LDA estimation model performed the best among the three models in detecting AMB asymptomatic pixels, while all the models were able to detect the symptomatic class. LDA correctly classified asymptomatic pixels and LDA model predicted them with an accuracy of 88.0%. An accuracy of 91.4% was achieved as the total classification accuracy. The results from this work indicate the potential of using the LDA estimation model to identify asymptomatic pixels on leaves infected by AMB.
基金supported by the national natural Science Foundation of China (31801397)the Henan Province Young Talents Lifting Project,China (2018HYTP003)+2 种基金the Independent Innovation Foundation of Henan Academy of Agricultural Sciences,China (2019ZC13)the earmarked fund for China Agriculture Research System (CARS-13)the Henan Provincial Agriculture Research System,China (S2012-05)。
文摘Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F1 hybrid was obtained by crossing the Silihong(Slh) cultivar with Arachis duranensis(ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization(Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55;2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats;3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.
基金We thank the Department of Science and Technology-Science and Engineering Research Board(DST-SERB)(PDF/2017/001488),India,for financial support.
文摘Purple blotch disease of Allium spp. crops caused by Alternaria porri has remained a major concern in agriculture for both farmers and research fraternity as it severely damages the crops and drastically reduces the yield. The symptoms appear after 1–4 days of infection and bulb rot begin, and eventually turn into dark reddish-purple and then brownish/black lesions. Many factors like season, time of sowing, humidity and temperature, stage of crop, and plant architecture have a huge impact on the progression of purple blotch disease. Many genic markers based on amplification of an Alta1 gene sequence have been designed for identification and differentiation of different Alternaria species groups. Among the most commonly used fungicides, mancozeb, tebuconazole, difenaconazole and azoxystrobin were found to be the ideal for the management of purple blotch disease and increased garlic yield. Many biological approaches such as plant extracts and bio-control agents were found partially effective for controlling the disease. A report on QTL mapping for purple blotch resistance discovered that purple blotch resistance is controlled by a single dominant gene ApR1. To completely understand the purple blotch disease resistance for crop improvement, a study is required at transcriptome level for hunting purple blotch resistant genes by gene annotation and mining. Genetic engineering and genome editing are other approaches that can be done for engineering disease resistance in Allium crops for genetic improvement.
基金Supported by the fund of Science and Technology Department of Hebei Province(13226511)the fund of Chengde Municipal Finance Bureau(CZ2014002,CZ2013007)the fund of Chengde Municipal Science and Technology Bureau(20157029)
文摘In the present study, agar diffusion test and field trials were carried out using a mushroom （Pleurotus ostreatus） culti- var Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasfi and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom, but also greatly promoted mushroom mycelial growth. Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.
基金Supported by Genetic Breeding Post Special Fund of Peanut Industrial Technology System in Henan Province(S2012-05-G01)Major Science and Technology Program of Henan Province(141100110600,161100111000)~~
文摘Shanghua 511 is a high-yield, high-oil and web blotch-resistant big peanut variety bred by the Shangqiu Academy of Agriculture and Forestry Sciences through sexual hybridization （Yuhua 18♀×Shangyan 9658♂）. In the wheat-peanut interplanting regional experiment conducted in Henan Province during 2012-2013, the average pod and seed yield of Shanghua 511 were 5906.19 and 4149.3 kg/hm2, respectively. In the wheat-peanut interplanting production experiment conducted in Henan Province in 2013, the average pod and seed yield of Shanghua 511 were 6036.45 and 4309.50 kg/hm2, respectively. In the kernels of Shanghua 511, the contents of crude fat, oleic acid and linoleic acid were 56.62%, 44.9% and 33.4% respectively, and the oleic/linoleic acid ratio was 1.34. This variety had high resistance to web blotch and medium resistance to leaf spot, viral diseases, root rot and collar rot. The 100-pod weight, 100-kernel weight, shelling percentage and growth period of Shanghua 511 were 270.6, 110.9 g, 70.6% and 120 d, respectively. Shanghua 511 passed the approval of Henan Provincial Crop Variety Approval Committee in August, 2015, and is suitable for spring sowing and wheat interplanting in the surrounding area of Henan Province and the area to the north of Huaihe River.
文摘This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight different onion growing areas were characterized in terms of cultural and morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish, light grey to whitish, deep greenish white, light grey and dirty white to greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate, raised and flat type colony elevation. Colony textures were cottony, fluffy and velvety with entire, undulate and filiform type colony margin. Among the culture media, V-7 juice agar found to be the most suitable culture media for mycclial growth of S. vesicarium. The sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato dextrose agar （PDA） media, this media exhibited the highest sporulation （87.76-169.0/mm^2） of S. vesicariumin comparison with other media. The minimum days （28 d to 31 d） for conidial production were observed on V-7 juice agar medium. The length of conidia varied from 14.6 μm to 30.6 μm. The maximum mean length of conidia was 29.97 μm found in isolate DSSA, while the minimum mean length 17.36 μm was found in isolate MSMM 02. The breadth of conidia ranged from 4.7 μm to 15.7μm. The maximum mean breadth of conidia was 12.55 μm found in the isolate DSSA, while the minimum mean breadth 9.760 μm was found in the isolate CCKH 02. The horizontal septation varied from l to 3 and the longitudinal septation varied from 0 to 4.
文摘An experiment was carried out to study the outcome of environmental factors (Temperature, Relative Humidity, Rainfall) and<span style="font-family:;" "=""> </span><span style="font-family:Verdana;">management of the purple blotch disease of onion using five treatments (Rovral + Ridoil gold with 3,4,5,6 sprays and control). The Taherpuri variety was used during the 2015-2016 cropping seasons at BARI, Gazipur, Bangladesh.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">In the experiment, the purple blotch disease surfaced at the end of January</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">2016 and was severe in late February to March (PDI 76.12%) due to temperature and RH above 24</span><span style="font-family:Verdana;">°C and 95%, respectively.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">As for the chemical control, the PDI for the treatments ranged from 50.66% to 83.33%.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">Significantly higher stem height was recorded by T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (68.06 cm), where six sprays of Rovral + Ridomil were applied. The minimum stem height was recorded in T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (59.23 cm). The maximum stem number per hill was recorded in T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (2.30) numerically but differed significantly with T</span><sub><span style="font-family:Verdana;">5</span></sub></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">(2.00), where fungicides were not applied. The maximum lesion area was measured in T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (25.26 mm</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">). Regarding PDI (Percent disease index), there was a significant difference among the treatments. The PDI value on purple blotch of onion ranged from 34.00 to 83.33. The lowest PDI was assessed in T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (34.00). Statistical analysis revealed that a significant decrease of PDI on purple blotch was recorded under treatments of T</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;">, T</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">, T</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, and T</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (39.20</span></span><span style="font-family:Verdana;">%</span><span style="font-family:Verdana;">, 45.60</span><span style="font-family:Verdana;">%</span><span style="font-family:Verdana;">, 49.59</span><span style="font-family:Verdana;">%</span><span style="font-family:;" "=""><span style="font-family:Verdana;">, and 59.19%) on controlling the disease and giving the optimum yield but all of them significantly differed with T</span><sub><span style="font-family:Verdana;">5</span></sub><span style="font-family:Verdana;"> (83.33%) where no fungicides were sprayed.</span></span>
文摘Ten different treatments viz. T<sub>0</sub> = Control, T<sub>1</sub> = Micronutrients (ZnSO<sub>4</sub> + Borax), T<sub>2</sub> = Rovral 50 WP + Micronutrients, T<sub>3</sub> = Dithane M-45 + Micronutrients, T<sub>4</sub> = Ridomil Gold + Micronutrients, T<sub>5</sub> = Cupravit + Micronutrients, T<sub>6</sub> = Rovral 50 WP, T<sub>7</sub> = Dithane M-45, T<sub>8</sub> = Ridomil Gold and T<sub>9</sub> = Cupravit were evaluated against the purple blotch complex disease of onion caused by Alternaria porri and Stemphylium vesicarium in field condition for bulb yield. In lab condition, all the tested chemical fungicides (Rovral 50 WP, Dithane M-45, Ridomil Gold MZ-72 and Cupravit 50 WP) significantly reduced mycelial growth of both pathogens. Among the fungicides, Rovral 50 WP @ 0.2% reduced the highest radial mycelial growth followed by Dithane M-45 @ 0.45% and Ridomil Gold MZ-72 @ 0.2% compared to control. In field experiment, application of Rovral 50 WP @ 0.2% along with micronutrients showed significant effect in reducing disease incidence and severity which significantly increased bulb yield and yield contributing characters.
文摘Reactions 13 wheat cultivars to 18 Mycosphaerella graminicola isolates, collected from three distinct localities, were in average different but differences among individual cultivars were small. All cultivars were susceptible to septoria tritici blotch. Reactions of wheat cultivars to M. graminicola isolates varied from 31% to 41%. Some cultivars had low differences but the other high in reactions to used isolates in average. Variance of individual cultivars to all isolates in reactions of maximal and minimal values fluctuated between 44-80%. More differences were in reactions between individual isolates to single cultivars. Aggressiveness individual isolates to all tested cultivars oscillated in average from 9% to 66% of necrotic leaf area covered by pycnidia. Three isolates had very low, whereas five isolates had high aggressiveness in average to all cultivars. Difference in maximal and minimal aggressiveness of individual isolates to all cultivars was between 13 and 60%. Avirulent reaction was ascertained in the cultivar Rexia to one isolate. Isolates showed wide differences in colored and morphological characteristics. Dissimilarity and similarity was in border shape, cultivation types, surface and coloration of isolate colony also.
文摘Both grain yield and disease performance are important factors to consider for winter wheat （Triticum aestivum） cultivar selection. However, disease index and yield data are often presented separately, making it difficult to compare values across multiple environments. Two-year investigations （2009-2010）, in which eight common wheat lines/genotypes were included to test their susceptibility against wheat LR （leaf rust） Puccinia triticina f. sp. tritici Roberge ex Desm., and the SLB （SeptoriaJStagonospora leaf blotch） complex Septoria tritici Desm. and Stagonospora nodorum, were carried out in the experimental field of ATTC （Agriculture Technology Transfer Centre） of Lushnje （Albania）. The objective of this study was to use a rank-based method to compare cultivars based on yield and disease performance combined across multiple environments. Analysis of variance revealed the presence of an important and significant variability in the experimental materials used to evaluate the susceptibility and the resistance of common wheat lines against SLB and LR. There were high negative correlations between yieldS, tritici （0.6683） and yield--P, recondita f. sp. tritici （0.5261）. The negative effects of two pathogens have shown ＂the parallel＂/similar negative influences on yield trait and there was a high positive correlation （0.7631） between S. tritici and P. recondita f. sp. tritici. According to study results the lines Regina × L-776, IKB-P6 and Bullgar 3 × KB 703 have shown good results of resistance （R） level against S. tritici and P. recondita f. sp. tritici.
基金Supported by Fundamental Research Project of Chongqing Academy of Forestry"Control Technologies against Major Pests and Diseases in Eucalyptus Planta-tion"(BB1102)Project of Chongqing Science and Technology Commission"Chongqing Key Laboratory of the Three Gorges Area Forest Ecology Protectionand Restoration"(CSTC,2007CA1001)
文摘[Objective] The paper was to explore safe fungicides with high efficiency and long persistence against eucalyptus purple blotch. [Method]10%Difenoconazole WG,500 g /L thiophanate-methyl SC and 50% carbendazim WP were evaluated through experiment in forest,and clear water was used as CK. [Result]Three fungicides all had relatively good control effect against eucalyptus purple blotch. Most eucalyptus trees were able to restore growth and sprout new shoots after continuous three times of administration with the interval of 10 d. 10% Difenoconazole WG possessed the best control effect against eucalyptus purple blotch,with the disease index of 25. 24 and the control effect of 40. 24%; followed by 500 g /L thiophanate-methyl SC 600 times dilution,with the disease index of 27. 09 and the control effect of 35. 87%; 50% carbendazim WP 500 times dilution had the poorest control effect,with the disease index of 32. 21 and the control effect of23. 74%. The disease index of CK was 42. 24. [Conclusion]The above fungicides were safe to eucalyptus,and could be used to control eucalyptus purple blotch.
文摘瓜类细菌性果斑病(bacterial fruit blotch)是瓜类作物上重要的种传细菌性病害,病原菌为西瓜噬酸菌Acidovorax citrulli。我国是全球西甜瓜的主要生产区。近年来,瓜类细菌性果斑病的频繁发生已严重影响我国西甜瓜产业的健康发展。为明确瓜类细菌性果斑病在我国的适生性,根据其在全球的最新分布数据,本研究利用MaxEnt模型结合ArcGIS软件预测了瓜类细菌性果斑病在我国的潜在地理分布。结果表明,MaxEnt模型的平均AUC(area under curve,AUC)值均大于0.9,预测结果的准确性较高。在历史气候条件下,瓜类细菌性果斑病适生区分布广泛,主要包括华中、华南和华东地区,以及部分华北、东北地区,占我国面积的47.36%。影响瓜类细菌性果斑病在我国潜在分布区域的主要气候因子包括最热月份最高温度、月平均昼夜温差、最干月份降水量和最干季平均温度。未来气候情景无论是低环境强迫还是高环境强迫,适生区面积均呈现增长的趋势,预示着随着气候的变化,瓜类细菌性果斑病在我国发生的风险不断增加,因此建议应加强检疫监测和防控,严防其扩散。
基金The authors express their appreciation to the Deanship of Research and Graduate Studies at King Khalid University for funding this work under grant number RGP2/233/45the support provided by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research at King FaisalUniversity,Saudi Arabia,under Project No.KFU242905funding from Princess Nourah bint Abdulrahman University through the Research Supporting Project Number PNURSP2025R241 in Riyadh,Saudi Arabia.
文摘Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from climate change can affect the severity of plant diseases.Therefore,two primary objectives of integrated disease management regarding climate change are identifying cultivars resistant to foliar diseases and understanding disease progression under abiotic stress.In the current study,we assessed the quantitative foliar disease resistance of 17 commercial barley cultivars under both normal and water stress conditions over two growing seasons(from 2020/21 to 2021/22).The findings demonstrated a reduced incidence of foliar fungal diseases(leaf rust,net blotch,and powdery mildew)under severe drought stress relative to standard irrigated field conditions.The barley cultivars(Giza 130,Giza 131,and Giza 133)demonstrated significant differences across all disease resistance indices.In addition,the study aimed to molecularly characterize 17 commercial barley varieties using single-cell DNA testing(SCoT)to identify genetic polymorphism and specific markers for each genotype.Eight SCoT primers were employed to investigate the genetic polymorphism among 17 barley varieties.Furthermore,these cultivars exhibited optimal performance for the majority of agricultural attributes examined,both under normal and water-stressed conditions.
基金supported by the National Natural Science Foundation of China(Grant No.32030095)the Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302).
文摘Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii),possessing significant aesthetic value and playing a crucial role in the species'reproduction and fitness.Despite years of research,deciphering the molecular mechanisms underlying blotch formation remains challenging.As is well known,floral pigmentation is frequently associated with the familiar R2R3-MYB transcription factors.The key MYB anthocyanin activators of P.rockii‘Shu Sheng Peng Mo’were previously reported in our preceding study.In this study,we identified and characterized three R2R3-MYBs,PrMYBi1,PrMYBi2,and PrMYBi3,which belong to subgroup 4(SG4)and play repressor roles in anthocyanin biosynthesis.A quantitative real-time PCR(qRT-PCR)assay indicated that the expression of PrMYBi1 and PrMYBi3 gradually increased during flowering development and was substantially up-regulated in non-blotch compared to blotch.Yeast one-hybrid and dualluciferase assays demonstrated that PrMYBi(1e3)directly target the anthocyanin structural genes and repress their transcription.The genetic transformation of tobacco demonstrated that the overexpression of PrMYBi(1e3)decreased anthocyanin accumulation in flowers,with PrMYBi1 serving as the most effective repressor.Our results revealed that SG4 R2R3-MYBs negatively regulate the anthocyanin pathway in P.rockii conservatively,and we provide the definite members.These findings will advance future research to unravel the mystery of blotch pattern formation.
