[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone ...[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone chicken. [Method] The wing venous blood of Donglan Black-bone chicken（silky feather and contour feather） was collected, and the total DNA was extracted. The 18 microsatellite loci were amplified by PCR technology, and the obtained gene fragments were scanned. The number of alleles（Na）, number of effective alleles（Ne）, heterozygosity（H） and polymorphism information content（PIC） were determined using Pop Gen 32 biological software to do cluster analysis of Guangxi indigenous chicken breeds. [Result] The18 microsatellite loci of Donglan black-bone chicken were found to be highly polymorphic. A total of 98 alleles were detected out from 80 Donglan black-bone chicken samples. The averages of Na, Ne, PIC and H per locus were 5.44, 2.78, 0.5382 and 0.596 3, respectively. A total of 93 and 98 alleles were detected out from silky- and contour-feather Donglan black-bone chickens, respectively. The averages of Ne per locus were 2.62 and 2.76, of PIC per locus were 0.508 8 and 0.539 8,of H per locus were 0.624 7 and 0.599 1, respectively. The results of cluster analysis showed that there was greater genetic distance between Donglan black-bone chicken and any of other Guangxi indigenous chicken breeds, including three yellow chicken, Xiayan chicken and Nandan Yao chicken, which was consistent with the breed evolution, geographical distribution and breeding history of Donglan black-bone chicken. [Conclusion] There are greater genetic variation and abundant genetic diversity in the conserved population of Donglan black-bone chicken, suggesting abundant genetic diversity and great breeding potential of Donglan black-bone chicken.展开更多
In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chick...In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chicken. According to the results, there were three genotypes （AA, AB and BB） of NPYgene in Donglan black-bone chicken group. Different genotypes exhibited significant effects （P 〈 0. 05 ） on the total number of eggs at 300 days of age. The total number of eggs at 300 days of age of AA genotype was significantly higher than that of BB genotype （P 〈 0. 05）. Therefore, the polymorphic site of NPY gene could be used as a candidate molecular marker that affects egg laying in Donglan black-bone chicken.展开更多
[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chicke...[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chickens were selected and divided into 4 groups,with 6 replicates in each group,with 20 pieces in each replicate.Among them,group A was the control group and was fed the basal diet;the groups B,C,and D were the experimental groups,which were separately supplemented with 0.25%,0.50%and 1.00%of Chinese herbal medicine additives in the basal diet,and the experimental period was 70 d.At the end of the feeding experiment,24 experimental chickens with similar body weight were selected from each group,and the same design of the feeding experiment was used to continue to feed the original diet for the metabolic experiment to determine the nutrient metabolic rate.[Results]Except the 50-70 d stage,the average daily gain(ADG)of group B in other stages was increased by 5.00%-9.15%(P<0.05),and the average body weight of group B at 70 d was also increased by 4.86%(P<0.05);the ADG of group C at 36-49,1-49 and 1-70 d and the average individual weight at 70 d were also higher than that of group A by 5.90%,2.66%,2.68%and 2.57%,respectively(P>0.05);at 36-49 d,the average daily feed intake(ADFI)of group A was higher than that of group B by 6.04%(P<0.01),and was also higher than that of groups C and D by 5.12%and 4.13%(P<0.05),respectively;at 1-49 d,the ADFI of group A was higher than that of group C by 2.86%(P<0.05),and there was no significant difference between the four groups of experimental chickens at each stage(P>0.05);at 22-35 and 1-70 d,the feed to gain ratio(F/G)of group B was 6.32%and 3.41%lower than that of group A(P<0.05),and at 36-49 and 1-49 d,the F/G of group B was even lower;at 36-49 d,the F/G of group C was 10.15%lower than that of group A(P<0.05),and at 1-49 d,it was also lower than that of group A by 5.29%(P<0.01);there was no significant difference between the two groups at other stages.In terms of nutrient metabolism:the three different amounts of this additive have increased the metabolic rate of DM,CP,CF,NFE,CA,Ca,P,TE and other nutrients in the diet to varying degrees,especially the addition of 0.25%and 0.50%had the significant increase.[Conclusions]The addition of 0.25%Chinese herbal medicine additives in the diet can significantly improve the growth performance and main nutrient metabolic rate of Donglan Black-bone Chickens.展开更多
This study was designed to investigate the effects of Fermented Broussonetia papyrifera(FBP)on growth performance,meat quality,and the expression of meat quality-related genes in Taihe black-bone silky fowl(TBsf).In t...This study was designed to investigate the effects of Fermented Broussonetia papyrifera(FBP)on growth performance,meat quality,and the expression of meat quality-related genes in Taihe black-bone silky fowl(TBsf).In the present study,360 ninety-day-old TBsfs(body weight=781±0.85 g)were divided into 4 groups with 5 replicates(18 hens per replicate),of which the control group was fed the basal diet and the 3 experimental groups were supplemented with 2%,4%,and 8%FBP.After 75 days,8 TBsfs in each group were randomly selected to collect their left pectoral muscles for meat quality and transcriptome analysis.Compared with the control group,4%FBP improved the growth performance and meat quality of TBsf,evidenced by the increasing trend in the ratio of feed intake to body weight gain(P=0.082),and significantly increased equivalent umami concentration(EUC),amino acid,and nucleotide compositions(P<0.05).In addition,the remaining meat quality indices including the yellowness of meat color,muscle fiber density,and shear force were also improved in the 4%group(P<0.05),with consistent improvements in the 8%FBP group.The transcriptome results indicated that FBP may regulate muscle fiber growth and development through the Forkhead box O(FoxO)and mitogen-activated protein kinase(MAPK)signaling pathways,and regulate meat color through the adipocytokine signaling pathway.Weighted gene coexpression network analysis(WGCNA)further revealed the candidate genes involved in amino acid metabolism and nucleotide metabolism,and the trends of these genes were inflecting with the 4%FBP group.Together,these results suggest that FBP significantly improved the growth performance,and meat quality of TBsf,with 4%FBP as the optimal addition ratio,and the transcriptome analysis revealed the mechanism of gene regulation.展开更多
目的:探讨黑玉米多糖(Black corn polysaccharide,BPS)对糖尿病性骨质疏松(Diabetic osteoporosis,DOP)大鼠的治疗作用及其机制。方法:采用高脂饮食联合链脲佐菌素构建DOP大鼠模型,随机分为模型组、BPS低剂量组和BPS高剂量组、二甲双胍...目的:探讨黑玉米多糖(Black corn polysaccharide,BPS)对糖尿病性骨质疏松(Diabetic osteoporosis,DOP)大鼠的治疗作用及其机制。方法:采用高脂饮食联合链脲佐菌素构建DOP大鼠模型,随机分为模型组、BPS低剂量组和BPS高剂量组、二甲双胍组,另设对照组。使用血糖仪测定大鼠空腹血糖(Fasting bloodglucose,FBG)、饮食饮水量、体重。采集血清、股骨、胫骨样本;ELISA法测定血清大鼠糖化血红蛋白(Glycosylated hemoglobin,GHb)、大鼠1,25二羟基维生素D3(1,25-(OH)2D3)、大鼠甲状旁腺激素(Parathyroid hormone,PTH)、大鼠Ⅰ型前胶原氨基端原肽(N-terminal propeptide of type Ⅰ procollagen,PINP)、大鼠抗酒石酸酸性磷酸酶5b(Tartrate-resistant acid phosphatase 5b,TRACP-5b)、大鼠Ⅰ型胶原交联羧基端肽(Type Ⅰ collagen cross-links the carboxy-terminal peptide,CTX-Ⅰ)、大鼠骨保护素(Osteoprotegerin,OPG)和大鼠核因子κB受体活化因子配体(Receptor activator of nuclear factor-κB ligand,RANKL)含量。通过HE染色观察进行皮质骨和松质骨的骨形态计量学测量。三点弯曲实验测股骨生物力学参数。免疫组化法观察胫骨中OPG、RANKL蛋白表达水平。结果:BPS可降低DOP大鼠的GHb(P<0.01)、FBG(P<0.01),下调血清中TRACP-5b(P<0.01)、CTX-I(P<0.01)水平并上调PTH(P<0.05)、1,25-(OH)_(2)D_(3)(P<0.01)、PINP、OPG、OPG/RANKL(P<0.05或P<0.01)水平。此外,BPS可上调大鼠胫骨上段静态参数骨小梁面积百分数(Trabecular area,%Tb.Ar)、骨小梁数量(Number of trabeculae,Tb.N)、皮质骨面积(Cortical bone area,Ct.Ar)和骨髓腔面积百分数(Area of bone marrow cavity,%Ma.Ar)值并减少骨小梁分离度(Trabecular bone dissociation,Tb.Sp)值。同时,BPS可增加大鼠股骨的最大载荷(P<0.01)、最大能量吸收(P<0.01),骨结构的韧性(P<0.01)和股骨的弹性模量(P<0.01)。另外,BPS会增强大鼠骨组织中OPG的表达,并抑制RANKL的表达。结论:BPS可有效降低血糖,改善骨代谢紊乱,提高骨强度,从而发挥抗DOP的作用,其机制可能与OPG/RANK/RANKL信号有关,为DOP的药物研发提供一定的理论依据。展开更多
基金Supported by Guangxi Natural Science Foundation(2013GXNSFDA019013)Fundamental Research Fund of Guangxi Institute of Animal Science(GMYK2014-05)~~
文摘[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone chicken. [Method] The wing venous blood of Donglan Black-bone chicken（silky feather and contour feather） was collected, and the total DNA was extracted. The 18 microsatellite loci were amplified by PCR technology, and the obtained gene fragments were scanned. The number of alleles（Na）, number of effective alleles（Ne）, heterozygosity（H） and polymorphism information content（PIC） were determined using Pop Gen 32 biological software to do cluster analysis of Guangxi indigenous chicken breeds. [Result] The18 microsatellite loci of Donglan black-bone chicken were found to be highly polymorphic. A total of 98 alleles were detected out from 80 Donglan black-bone chicken samples. The averages of Na, Ne, PIC and H per locus were 5.44, 2.78, 0.5382 and 0.596 3, respectively. A total of 93 and 98 alleles were detected out from silky- and contour-feather Donglan black-bone chickens, respectively. The averages of Ne per locus were 2.62 and 2.76, of PIC per locus were 0.508 8 and 0.539 8,of H per locus were 0.624 7 and 0.599 1, respectively. The results of cluster analysis showed that there was greater genetic distance between Donglan black-bone chicken and any of other Guangxi indigenous chicken breeds, including three yellow chicken, Xiayan chicken and Nandan Yao chicken, which was consistent with the breed evolution, geographical distribution and breeding history of Donglan black-bone chicken. [Conclusion] There are greater genetic variation and abundant genetic diversity in the conserved population of Donglan black-bone chicken, suggesting abundant genetic diversity and great breeding potential of Donglan black-bone chicken.
基金Supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region(2013jj DA30049)
文摘In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chicken. According to the results, there were three genotypes （AA, AB and BB） of NPYgene in Donglan black-bone chicken group. Different genotypes exhibited significant effects （P 〈 0. 05 ） on the total number of eggs at 300 days of age. The total number of eggs at 300 days of age of AA genotype was significantly higher than that of BB genotype （P 〈 0. 05）. Therefore, the polymorphic site of NPY gene could be used as a candidate molecular marker that affects egg laying in Donglan black-bone chicken.
基金Supported by National Modern Agricultural Industry Technology System Guangxi Broiler Innovation Team Building Project(nycytxgxcxtd-19-01)Guangxi Agricultural Science and Technology Self-financing Project(Z2022114).
文摘[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chickens were selected and divided into 4 groups,with 6 replicates in each group,with 20 pieces in each replicate.Among them,group A was the control group and was fed the basal diet;the groups B,C,and D were the experimental groups,which were separately supplemented with 0.25%,0.50%and 1.00%of Chinese herbal medicine additives in the basal diet,and the experimental period was 70 d.At the end of the feeding experiment,24 experimental chickens with similar body weight were selected from each group,and the same design of the feeding experiment was used to continue to feed the original diet for the metabolic experiment to determine the nutrient metabolic rate.[Results]Except the 50-70 d stage,the average daily gain(ADG)of group B in other stages was increased by 5.00%-9.15%(P<0.05),and the average body weight of group B at 70 d was also increased by 4.86%(P<0.05);the ADG of group C at 36-49,1-49 and 1-70 d and the average individual weight at 70 d were also higher than that of group A by 5.90%,2.66%,2.68%and 2.57%,respectively(P>0.05);at 36-49 d,the average daily feed intake(ADFI)of group A was higher than that of group B by 6.04%(P<0.01),and was also higher than that of groups C and D by 5.12%and 4.13%(P<0.05),respectively;at 1-49 d,the ADFI of group A was higher than that of group C by 2.86%(P<0.05),and there was no significant difference between the four groups of experimental chickens at each stage(P>0.05);at 22-35 and 1-70 d,the feed to gain ratio(F/G)of group B was 6.32%and 3.41%lower than that of group A(P<0.05),and at 36-49 and 1-49 d,the F/G of group B was even lower;at 36-49 d,the F/G of group C was 10.15%lower than that of group A(P<0.05),and at 1-49 d,it was also lower than that of group A by 5.29%(P<0.01);there was no significant difference between the two groups at other stages.In terms of nutrient metabolism:the three different amounts of this additive have increased the metabolic rate of DM,CP,CF,NFE,CA,Ca,P,TE and other nutrients in the diet to varying degrees,especially the addition of 0.25%and 0.50%had the significant increase.[Conclusions]The addition of 0.25%Chinese herbal medicine additives in the diet can significantly improve the growth performance and main nutrient metabolic rate of Donglan Black-bone Chickens.
基金supported by the fund from the Key Research and Development Program of Ji'an city(Taihe Silky Fowl)and Jiangxi province(20223BBF61006).
文摘This study was designed to investigate the effects of Fermented Broussonetia papyrifera(FBP)on growth performance,meat quality,and the expression of meat quality-related genes in Taihe black-bone silky fowl(TBsf).In the present study,360 ninety-day-old TBsfs(body weight=781±0.85 g)were divided into 4 groups with 5 replicates(18 hens per replicate),of which the control group was fed the basal diet and the 3 experimental groups were supplemented with 2%,4%,and 8%FBP.After 75 days,8 TBsfs in each group were randomly selected to collect their left pectoral muscles for meat quality and transcriptome analysis.Compared with the control group,4%FBP improved the growth performance and meat quality of TBsf,evidenced by the increasing trend in the ratio of feed intake to body weight gain(P=0.082),and significantly increased equivalent umami concentration(EUC),amino acid,and nucleotide compositions(P<0.05).In addition,the remaining meat quality indices including the yellowness of meat color,muscle fiber density,and shear force were also improved in the 4%group(P<0.05),with consistent improvements in the 8%FBP group.The transcriptome results indicated that FBP may regulate muscle fiber growth and development through the Forkhead box O(FoxO)and mitogen-activated protein kinase(MAPK)signaling pathways,and regulate meat color through the adipocytokine signaling pathway.Weighted gene coexpression network analysis(WGCNA)further revealed the candidate genes involved in amino acid metabolism and nucleotide metabolism,and the trends of these genes were inflecting with the 4%FBP group.Together,these results suggest that FBP significantly improved the growth performance,and meat quality of TBsf,with 4%FBP as the optimal addition ratio,and the transcriptome analysis revealed the mechanism of gene regulation.
文摘目的:探讨黑玉米多糖(Black corn polysaccharide,BPS)对糖尿病性骨质疏松(Diabetic osteoporosis,DOP)大鼠的治疗作用及其机制。方法:采用高脂饮食联合链脲佐菌素构建DOP大鼠模型,随机分为模型组、BPS低剂量组和BPS高剂量组、二甲双胍组,另设对照组。使用血糖仪测定大鼠空腹血糖(Fasting bloodglucose,FBG)、饮食饮水量、体重。采集血清、股骨、胫骨样本;ELISA法测定血清大鼠糖化血红蛋白(Glycosylated hemoglobin,GHb)、大鼠1,25二羟基维生素D3(1,25-(OH)2D3)、大鼠甲状旁腺激素(Parathyroid hormone,PTH)、大鼠Ⅰ型前胶原氨基端原肽(N-terminal propeptide of type Ⅰ procollagen,PINP)、大鼠抗酒石酸酸性磷酸酶5b(Tartrate-resistant acid phosphatase 5b,TRACP-5b)、大鼠Ⅰ型胶原交联羧基端肽(Type Ⅰ collagen cross-links the carboxy-terminal peptide,CTX-Ⅰ)、大鼠骨保护素(Osteoprotegerin,OPG)和大鼠核因子κB受体活化因子配体(Receptor activator of nuclear factor-κB ligand,RANKL)含量。通过HE染色观察进行皮质骨和松质骨的骨形态计量学测量。三点弯曲实验测股骨生物力学参数。免疫组化法观察胫骨中OPG、RANKL蛋白表达水平。结果:BPS可降低DOP大鼠的GHb(P<0.01)、FBG(P<0.01),下调血清中TRACP-5b(P<0.01)、CTX-I(P<0.01)水平并上调PTH(P<0.05)、1,25-(OH)_(2)D_(3)(P<0.01)、PINP、OPG、OPG/RANKL(P<0.05或P<0.01)水平。此外,BPS可上调大鼠胫骨上段静态参数骨小梁面积百分数(Trabecular area,%Tb.Ar)、骨小梁数量(Number of trabeculae,Tb.N)、皮质骨面积(Cortical bone area,Ct.Ar)和骨髓腔面积百分数(Area of bone marrow cavity,%Ma.Ar)值并减少骨小梁分离度(Trabecular bone dissociation,Tb.Sp)值。同时,BPS可增加大鼠股骨的最大载荷(P<0.01)、最大能量吸收(P<0.01),骨结构的韧性(P<0.01)和股骨的弹性模量(P<0.01)。另外,BPS会增强大鼠骨组织中OPG的表达,并抑制RANKL的表达。结论:BPS可有效降低血糖,改善骨代谢紊乱,提高骨强度,从而发挥抗DOP的作用,其机制可能与OPG/RANK/RANKL信号有关,为DOP的药物研发提供一定的理论依据。
