Non-injurious local stimuli, such as a cold shock, and injurious stimuli, such as local burning, punctures or chemicals, were applied to study electrical wave transmission in black pine (Pinus thunbergii) seedlings. T...Non-injurious local stimuli, such as a cold shock, and injurious stimuli, such as local burning, punctures or chemicals, were applied to study electrical wave transmission in black pine (Pinus thunbergii) seedlings. The results showed that non-injurious stimuli evoked the action potential (AP) transmission and injurious stimulation induced both AP transmission and the more complex variation (VP) transmission in the seedlings. The causes of these phenomena were discussed. Key words Black pine - Pinus thunbergii - Action potential (AP) - Variation potential (VP) - Electrical wave transmission CLC number S791.256 Document code A Foundation item: This paper was supported by the National Nature Science Foundation of China (grant No. 39670613).Biography: GUO Jian (1971-), male, lecturer in Haikou Bureau of Forestry. Hainan, P. R. China.Responsible editor: Song Funan展开更多
The alien woody legume, black locust （Robinia pseudoaca-cia）, has invaded Japanese black pine （Pinus thunbergii） forests located in Japan’s coastal plain and hill regions where gaps are formed in pine forests aft...The alien woody legume, black locust （Robinia pseudoaca-cia）, has invaded Japanese black pine （Pinus thunbergii） forests located in Japan’s coastal plain and hill regions where gaps are formed in pine forests after nematode infestation. Nitrogen fixation by legumes acceler-ates N cycling in forest ecosystems. We studied temporal change in the annual tree-ring resolution N stable isotope composition （δ15N, a per mil deviation of δ15N/14N ratio, relative to atmospheric N2δ15N=0‰） at two natural locations of Japanese black pine forest with black locust that differed in the time since black locust establishment （Shohnai in north-east and Kita-Kyushu in southwest Japan）. Analyzed tree-rings covered the period from 1990/1992 to 2009. N acquisition by Japanese black pine from black locust N input to the soil was evidenced by temporal shifting of N stable isotope composition on the annual pine tree rings. With pro-gressive development of the forest stand,δ15N values of earlier tree-ringsδ15N of -5‰） from black pine associated with black locust shifted to-wards values similar to those of black locustδ15N values nearly to-1‰）, which suggests acquisition of N by N2 fixation （Shohnai site）. In con-trast, in a forest where black locust had settled for two or three genera-tions, in a black pine stand （Kita-Kyushu site）, longer periods of N en-richment in the soil were reflected in the elevated tree-ringδ15N values of newly established black pine trees. Based on tree-ringδ15N data from the Shohnai site, we determined that about 10 years after black locust establishment, soil N had already been enriched by black locust N, this, in turn, contributed to N fertilization of surrounding trees in mixed stands.展开更多
In Italy,the hydro-geological protection of the Apennine territories is a well-known issue.For this reason,at the end of the 19th century,a new reforestation technique was proposed by the Inspector of Forest Service,P...In Italy,the hydro-geological protection of the Apennine territories is a well-known issue.For this reason,at the end of the 19th century,a new reforestation technique was proposed by the Inspector of Forest Service,Pietro Montanari to cope with difficult soil conditions of the mountain areas.This new approach was the first application of the so-called"gradoni"(small terraces)method of soil preparation.Each"gradone"was 100-120 cm wide and made with a slight counter slope(ca 10-15 degrees)to promote the accumulation of water and organic matter.Along each"gradone",bare root black pine seedlings(Pinus nigra Am.)were planted at a distance of 1 m from each other with a density of more than 3,000/ha.Nowadays,19,158 hectares of black pine forests are growing in the Apennine of the Abruzzi region,largely resulting from those reforestation projects realized since the beginning of the 20th century.A case study of Monte Plaia(42°00′12″N 13°53′39″E),that illustrates the situation after 70-110 years in consequence of lacking of silvicultural treatment and the application of aforest restoration method is presented.展开更多
A specific and sensitive polymerase chain reaction (PCR) assay based on the internal transcribed spacer (ITS) region of rDNA sequences was developed to detect endophytic and phytopathogenic fungi from needles of the J...A specific and sensitive polymerase chain reaction (PCR) assay based on the internal transcribed spacer (ITS) region of rDNA sequences was developed to detect endophytic and phytopathogenic fungi from needles of the Japanese black pine, Pinus thunbergii. Sequences of the ITS regions of Lophodermium conigenum, Lecanosticta acicola, Pestalotiopsis neglecta, Rhizosphaera kalkhoffii, and Septorioides pini-thunbergii were compared, and each specific primer pair for these species was designed. First, the designed primer pairs were tested for their specificity to detect each species. A PCR product was amplified only each combination of species and its specific primer pair, confirming the specificity of the designed primer pairs. These primer pairs were also tested on DNA extracted from the needles of P. thunbergii. The PCR products were amplified not only in needles with lesions but also in healthy needles without symptoms. Furthermore, several endophytic and phytopathogenic fungi could be simultaneously detected from the same region in a needle. The PCR-mediated detection method developed in this study will be a valuable tool for the detection of the endophytic and phytopathogenic fungi, not only as a rapid diagnostic tool for early detection but also for monitoring variations in both the quality and quantity of the endophytic and phytopathogenic fungi in needles in Japanese black pines.展开更多
Many studies have estimated approximately ranges of thresholds of low soil temperature in the growth and ecophysi-ological traits of trees, but difficultly determined the exact values. To resolve the problem, black sp...Many studies have estimated approximately ranges of thresholds of low soil temperature in the growth and ecophysi-ological traits of trees, but difficultly determined the exact values. To resolve the problem, black spruce （Picea mariana） and jack pine （Pinus banksiana） seedlings were exposed to 5, 10, 15, 20, 25, 30 and 35℃ soil temperature in greenhouses. After 90 days of the treatment, net photosynthetic rate （A）, stomatal conductance （gs）, transpiration rate （E）, water use efficiency （WUE） and specific leaf area （SLA） were measured. This study showed that all the traits had an asymmetrical peak relationship with changing soil temperature, the relationship was well simulated using a cubic curvilinear model, and the exact thresholds could be derived from the second derivative of the model. The results revealed that the thresholds varied among ecophysiological traits and between tree species. In black spruce, the thresholds were 14.1, 14.7, 10.7, 14.4 and 16.2℃ forA, gs, E, WUE and SLA; 15.4, 10.4, 14.7, 16.9 and 10.5℃ for the corresponding traits in jack pine. The lowest thresholds of E in black spruce and gs in jack pine were an indicator representing the minimum requirement of soil temperature for the regular processes of ecophysiology. The highest thresholds of SLA in black spruce and WUE in jack pine suggest they are the most sensitive to decreasing soil temperature and may play an important role in the acclimation. The averaged thresholds were at 14.0 and 13.6℃ for black spruce and jack pine, suggesting that the sensitivity of both species to low soil temperature was quite close.展开更多
In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus g...In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be downregulated.In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system.Here in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite effect.In addition,RNAi of sft-4 changed the female–male ratio and lifespan of PWN.In bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly weakened.These results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.展开更多
Estimating individual tree volume is one of the essential building blocks in forest growth and yield models. Ecologically based taper equations provide accurate vol- ume predictions and allow classification by mer- ch...Estimating individual tree volume is one of the essential building blocks in forest growth and yield models. Ecologically based taper equations provide accurate vol- ume predictions and allow classification by mer- chantable sizes, assisting in sustainable forest management. In the present study, ecoregion-based compatible volume systems for brutian pine and black pine in the three ecoregions of southern Turkey were developed. Several well-known taper functions were evaluated. A second- order continuous-time autoregressive error structure was used to correct the inherent autocorrelation in the hierar- chical data, allowing the model to be applied to irregularly spaced and unbalanced data. The compatible segmented model of Fang et al. （For Sci 46：1-12, 2000） best described the experimental data. It is therefore recommended for estimating diameter at a specific height, height to a specific diameter, merchantable volume, and total volume for the three ecoregions and two species analyzed. The nonlinearextra sum of squares method indicated differences in ecoregion and tree-specific taper functions. A different taper function should therefore be used for each pine spe- cies and ecoregion in southern Turkey. Using ecoregion- specific taper equations allows making more robust esti- mations and, therefore, will enhance the accuracy of diameter at different heights and volume predictions.展开更多
We investigated the effects of precommercial thinning(PCT)applied at different levels on the growth parameters of natural Anatolian black pine stand.The experimental site was located in the province of Isparta in sout...We investigated the effects of precommercial thinning(PCT)applied at different levels on the growth parameters of natural Anatolian black pine stand.The experimental site was located in the province of Isparta in southern Turkey.PCT was set up using a randomized complete block design with three replicates and three treatments(control,2–2.5 m×–2.5 m and 3–3.5 m×–3.5 m spacing).The experimental plots were established in March 2014.At the end of the growing seasons for three years(2014–2016),growth parameters such as diameter,height and crown radius were measured.PCT was found to be significantly effective on diameter,height,individual tree basal area,and crown radius increment at the end of the third year(p<0.05).While diameter,individual tree basal area and crown radius increment increased with increasing PCT intensity,height increment was higher in control than PCT.Although the PCT treatments applied with 3–3.5 m spacing affected individual tree development positively,we need long-term results on wood quality and yield.According to the short-term results,it is thought that it is better to lower the stand closeness gradually,so,it may be advisable to apply PCT by 2–2.5 m×–2.5 m spacing.展开更多
基金This paper was supported by the National Nature Science Foundation of China (grant No. 39670613).
文摘Non-injurious local stimuli, such as a cold shock, and injurious stimuli, such as local burning, punctures or chemicals, were applied to study electrical wave transmission in black pine (Pinus thunbergii) seedlings. The results showed that non-injurious stimuli evoked the action potential (AP) transmission and injurious stimulation induced both AP transmission and the more complex variation (VP) transmission in the seedlings. The causes of these phenomena were discussed. Key words Black pine - Pinus thunbergii - Action potential (AP) - Variation potential (VP) - Electrical wave transmission CLC number S791.256 Document code A Foundation item: This paper was supported by the National Nature Science Foundation of China (grant No. 39670613).Biography: GUO Jian (1971-), male, lecturer in Haikou Bureau of Forestry. Hainan, P. R. China.Responsible editor: Song Funan
文摘The alien woody legume, black locust （Robinia pseudoaca-cia）, has invaded Japanese black pine （Pinus thunbergii） forests located in Japan’s coastal plain and hill regions where gaps are formed in pine forests after nematode infestation. Nitrogen fixation by legumes acceler-ates N cycling in forest ecosystems. We studied temporal change in the annual tree-ring resolution N stable isotope composition （δ15N, a per mil deviation of δ15N/14N ratio, relative to atmospheric N2δ15N=0‰） at two natural locations of Japanese black pine forest with black locust that differed in the time since black locust establishment （Shohnai in north-east and Kita-Kyushu in southwest Japan）. Analyzed tree-rings covered the period from 1990/1992 to 2009. N acquisition by Japanese black pine from black locust N input to the soil was evidenced by temporal shifting of N stable isotope composition on the annual pine tree rings. With pro-gressive development of the forest stand,δ15N values of earlier tree-ringsδ15N of -5‰） from black pine associated with black locust shifted to-wards values similar to those of black locustδ15N values nearly to-1‰）, which suggests acquisition of N by N2 fixation （Shohnai site）. In con-trast, in a forest where black locust had settled for two or three genera-tions, in a black pine stand （Kita-Kyushu site）, longer periods of N en-richment in the soil were reflected in the elevated tree-ringδ15N values of newly established black pine trees. Based on tree-ringδ15N data from the Shohnai site, we determined that about 10 years after black locust establishment, soil N had already been enriched by black locust N, this, in turn, contributed to N fertilization of surrounding trees in mixed stands.
文摘In Italy,the hydro-geological protection of the Apennine territories is a well-known issue.For this reason,at the end of the 19th century,a new reforestation technique was proposed by the Inspector of Forest Service,Pietro Montanari to cope with difficult soil conditions of the mountain areas.This new approach was the first application of the so-called"gradoni"(small terraces)method of soil preparation.Each"gradone"was 100-120 cm wide and made with a slight counter slope(ca 10-15 degrees)to promote the accumulation of water and organic matter.Along each"gradone",bare root black pine seedlings(Pinus nigra Am.)were planted at a distance of 1 m from each other with a density of more than 3,000/ha.Nowadays,19,158 hectares of black pine forests are growing in the Apennine of the Abruzzi region,largely resulting from those reforestation projects realized since the beginning of the 20th century.A case study of Monte Plaia(42°00′12″N 13°53′39″E),that illustrates the situation after 70-110 years in consequence of lacking of silvicultural treatment and the application of aforest restoration method is presented.
文摘A specific and sensitive polymerase chain reaction (PCR) assay based on the internal transcribed spacer (ITS) region of rDNA sequences was developed to detect endophytic and phytopathogenic fungi from needles of the Japanese black pine, Pinus thunbergii. Sequences of the ITS regions of Lophodermium conigenum, Lecanosticta acicola, Pestalotiopsis neglecta, Rhizosphaera kalkhoffii, and Septorioides pini-thunbergii were compared, and each specific primer pair for these species was designed. First, the designed primer pairs were tested for their specificity to detect each species. A PCR product was amplified only each combination of species and its specific primer pair, confirming the specificity of the designed primer pairs. These primer pairs were also tested on DNA extracted from the needles of P. thunbergii. The PCR products were amplified not only in needles with lesions but also in healthy needles without symptoms. Furthermore, several endophytic and phytopathogenic fungi could be simultaneously detected from the same region in a needle. The PCR-mediated detection method developed in this study will be a valuable tool for the detection of the endophytic and phytopathogenic fungi, not only as a rapid diagnostic tool for early detection but also for monitoring variations in both the quality and quantity of the endophytic and phytopathogenic fungi in needles in Japanese black pines.
基金supported by the Lakehead University Graduate Fellowship, Nature Science and Engineer Research Council Scholarship of Canada (NSERC) PGS A,the National Natural Science Foundation of China (Grant No. 30872000)K. C. Wong Education Foundation of Hong Kong (2008) and the funding initiative of Institute of Mountain Hazards and Environment, Chinese Academy of Sciences to the author and NSERC research grant to Qing-Lai Dang
文摘Many studies have estimated approximately ranges of thresholds of low soil temperature in the growth and ecophysi-ological traits of trees, but difficultly determined the exact values. To resolve the problem, black spruce （Picea mariana） and jack pine （Pinus banksiana） seedlings were exposed to 5, 10, 15, 20, 25, 30 and 35℃ soil temperature in greenhouses. After 90 days of the treatment, net photosynthetic rate （A）, stomatal conductance （gs）, transpiration rate （E）, water use efficiency （WUE） and specific leaf area （SLA） were measured. This study showed that all the traits had an asymmetrical peak relationship with changing soil temperature, the relationship was well simulated using a cubic curvilinear model, and the exact thresholds could be derived from the second derivative of the model. The results revealed that the thresholds varied among ecophysiological traits and between tree species. In black spruce, the thresholds were 14.1, 14.7, 10.7, 14.4 and 16.2℃ forA, gs, E, WUE and SLA; 15.4, 10.4, 14.7, 16.9 and 10.5℃ for the corresponding traits in jack pine. The lowest thresholds of E in black spruce and gs in jack pine were an indicator representing the minimum requirement of soil temperature for the regular processes of ecophysiology. The highest thresholds of SLA in black spruce and WUE in jack pine suggest they are the most sensitive to decreasing soil temperature and may play an important role in the acclimation. The averaged thresholds were at 14.0 and 13.6℃ for black spruce and jack pine, suggesting that the sensitivity of both species to low soil temperature was quite close.
基金supported by the Shandong Provincial Natural Science Foundation,China(ZR2020MC123)Qingdao Municipal People-benefitting Demonstration Project of Science and Technology,China(23-2-8-cspz-8-nsh).
文摘In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be downregulated.In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system.Here in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite effect.In addition,RNAi of sft-4 changed the female–male ratio and lifespan of PWN.In bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly weakened.These results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.
基金financially supported by the Scientific and Technological Research Council of Turkey(Project No:109 O 714)
文摘Estimating individual tree volume is one of the essential building blocks in forest growth and yield models. Ecologically based taper equations provide accurate vol- ume predictions and allow classification by mer- chantable sizes, assisting in sustainable forest management. In the present study, ecoregion-based compatible volume systems for brutian pine and black pine in the three ecoregions of southern Turkey were developed. Several well-known taper functions were evaluated. A second- order continuous-time autoregressive error structure was used to correct the inherent autocorrelation in the hierar- chical data, allowing the model to be applied to irregularly spaced and unbalanced data. The compatible segmented model of Fang et al. （For Sci 46：1-12, 2000） best described the experimental data. It is therefore recommended for estimating diameter at a specific height, height to a specific diameter, merchantable volume, and total volume for the three ecoregions and two species analyzed. The nonlinearextra sum of squares method indicated differences in ecoregion and tree-specific taper functions. A different taper function should therefore be used for each pine spe- cies and ecoregion in southern Turkey. Using ecoregion- specific taper equations allows making more robust esti- mations and, therefore, will enhance the accuracy of diameter at different heights and volume predictions.
基金This study was a part of the doctoral dissertation and supported by Suleyman Demirel University Projects(Project numbers:OYP-05241-DR14 and 4167-OYP-D2-14).
文摘We investigated the effects of precommercial thinning(PCT)applied at different levels on the growth parameters of natural Anatolian black pine stand.The experimental site was located in the province of Isparta in southern Turkey.PCT was set up using a randomized complete block design with three replicates and three treatments(control,2–2.5 m×–2.5 m and 3–3.5 m×–3.5 m spacing).The experimental plots were established in March 2014.At the end of the growing seasons for three years(2014–2016),growth parameters such as diameter,height and crown radius were measured.PCT was found to be significantly effective on diameter,height,individual tree basal area,and crown radius increment at the end of the third year(p<0.05).While diameter,individual tree basal area and crown radius increment increased with increasing PCT intensity,height increment was higher in control than PCT.Although the PCT treatments applied with 3–3.5 m spacing affected individual tree development positively,we need long-term results on wood quality and yield.According to the short-term results,it is thought that it is better to lower the stand closeness gradually,so,it may be advisable to apply PCT by 2–2.5 m×–2.5 m spacing.
