Emerging evidence suggests a link between alterations in the gut microbiome and adverse health outcomes in the hosts exposed to environmental pollutants.Yet,the causal relationships and underlying mechanisms remain la...Emerging evidence suggests a link between alterations in the gut microbiome and adverse health outcomes in the hosts exposed to environmental pollutants.Yet,the causal relationships and underlying mechanisms remain largely undefined.Here we show that exposure to biotoxins can affect gut pathobiome assembly in amphibians,which in turn triggers the toxicity of exogenous pollutants.We used Xenopus laevis as a model in this study.Tadpoles exposed to tropolone demonstrated notable developmental impairments and increased locomotor activity,with a reduction in total length by 4.37%e22.48%and an increase in swimming speed by 49.96%e84.83%.Fusobacterium and Cetobacterium are predominant taxa in the gut pathobiome of tropolone-exposed tadpoles.The tropolone-induced developmental and behavioral disorders in the host were mediated by assembly of the gut pathobiome,leading to transcriptome reprogramming.This study not only advances our understanding of the intricate interactions between environmental pollutants,the gut pathobiome,and host health but also emphasizes the potential of the gut pathobiome in mediating the toxicological effects of environmental contaminants.展开更多
Saxitoxin(STX)is a marine biotoxin produced by cyanobacteria and methanobacteria,which is harmful to humans and aquatic organisms.Therefore,it is urgent to establish an efficient,sensitive and selective method for the...Saxitoxin(STX)is a marine biotoxin produced by cyanobacteria and methanobacteria,which is harmful to humans and aquatic organisms.Therefore,it is urgent to establish an efficient,sensitive and selective method for the screening of STX.In this paper,based on the HAuCl_(4)/H_(2)O_(2)reaction system,gold nanoparticles(Au NPs)decorated Fe_(3)O_(4)magnetic beads(Fe3O4@Au NPs)and Pt/Au nanozymes(Pt/Au NZs),an improved surface-enhanced Raman scattering(SERS)aptasensor was proposed for sensitive and selective detection of STX.Firstly,SERS tag(4-mercaptobenzoic acid)and aptamer modified Fe3O4@Au NPs were hybridized with complementary DNA modified Pt/Au NZs(Pt/Au-cDNA)to form the specific complexes.Wherein,aptamer acted as capture agent to specifically recognize STX,Pt/Au NZs catalyzed HAuCl_(4)/H_(2)O_(2)reaction to enhance SERS signal,and Fe_(3)O_(4)facilitated the magnetic separation and simple detection operations.The SERS aptasensor achieved sensitive detection of STX with a wide linear range from 0.02 to 0.8μmol/L and a low limit of detection(LOD)of 13 nmol/L(signal-to-noise(S/N)=3).To evaluate the performance of this method,STX were detected in the spiked seafood samples with recoveries from 78.75%to 150%with relative standard deviation below 6.52%.Besides,the SERS aptasensor behaved good stability and repeatability,revealing its promising potential in STX detection and food safety detection.展开更多
Laguimit Bay is among the few areas in the Philippines where green mussels thrive.However,no studies have been conducted on Harmful Algal Bloom(HAB)in the area.This study investigates the spatial distribution of dinof...Laguimit Bay is among the few areas in the Philippines where green mussels thrive.However,no studies have been conducted on Harmful Algal Bloom(HAB)in the area.This study investigates the spatial distribution of dinoflagellates associated with HAB in the surface water of the bay.Multiparameter water quality testers were used to determine the physicochemical characteristics while a plankton net was used to filter the dinoflagellates and counting was carried out in a compound microscope.Based on the results,the surface water temperature is between 27–31℃ while the pH ranges from 8.1–8.2 and dissolved oxygen is 6.3–6.5 mg/L at salinity ranges from 30–31 ppt.This indicates that the water quality of the Bay is sustainable for farming mussels.Microscopic examination of the samples showed that the only dinoflagellate associated with HAB is Pyrodinium bahamense var.compressum which causes Paralytic Shellfish Poisoning with an average concentration of 22 cells/L.Other non-HAB species were Protoperidinium depressum with an average of 489 cells/L and the rest are Ceratium macroceros,Ceratium furca,Ceratium trichoceros,Ceratium lunula,Dinophysis caudata,Dinophysis miles.The Shannon-Wiener diversity index was 1.8723 in station 1 and 1.8406 in station 2 and 1.7439 in station 3 which indicates a low diversity index.The presence of P.bahamanse has the potential for recurrence of HAB formation in Laguimit Bay which could affect the economic livelihood of fisherfolk in the country.Continued monitoring and management are recommended to mitigate potential HAB risks and ensure the sustainability of shellfish resources.展开更多
基金supported by the National Natural Science Foundation of China(32122074)the National Key R&D Program of China(2023YFD1400901)the Zhejiang University Global Partnership Fund,and the Fundamental Research Funds for the Central Universities(2021FZZX001-31 and 226-2023-00070).
文摘Emerging evidence suggests a link between alterations in the gut microbiome and adverse health outcomes in the hosts exposed to environmental pollutants.Yet,the causal relationships and underlying mechanisms remain largely undefined.Here we show that exposure to biotoxins can affect gut pathobiome assembly in amphibians,which in turn triggers the toxicity of exogenous pollutants.We used Xenopus laevis as a model in this study.Tadpoles exposed to tropolone demonstrated notable developmental impairments and increased locomotor activity,with a reduction in total length by 4.37%e22.48%and an increase in swimming speed by 49.96%e84.83%.Fusobacterium and Cetobacterium are predominant taxa in the gut pathobiome of tropolone-exposed tadpoles.The tropolone-induced developmental and behavioral disorders in the host were mediated by assembly of the gut pathobiome,leading to transcriptome reprogramming.This study not only advances our understanding of the intricate interactions between environmental pollutants,the gut pathobiome,and host health but also emphasizes the potential of the gut pathobiome in mediating the toxicological effects of environmental contaminants.
基金supported by the National Natural Science Foundation of China(3236062242388101)+2 种基金the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202152)the Open Project of Key Laboratory of Tropical Fruits and Vegetables Quality and Safety,State Administration for Market Regulation(KF-2025001)the China Postdoctoral Science Foundation(2024M760311).
文摘Saxitoxin(STX)is a marine biotoxin produced by cyanobacteria and methanobacteria,which is harmful to humans and aquatic organisms.Therefore,it is urgent to establish an efficient,sensitive and selective method for the screening of STX.In this paper,based on the HAuCl_(4)/H_(2)O_(2)reaction system,gold nanoparticles(Au NPs)decorated Fe_(3)O_(4)magnetic beads(Fe3O4@Au NPs)and Pt/Au nanozymes(Pt/Au NZs),an improved surface-enhanced Raman scattering(SERS)aptasensor was proposed for sensitive and selective detection of STX.Firstly,SERS tag(4-mercaptobenzoic acid)and aptamer modified Fe3O4@Au NPs were hybridized with complementary DNA modified Pt/Au NZs(Pt/Au-cDNA)to form the specific complexes.Wherein,aptamer acted as capture agent to specifically recognize STX,Pt/Au NZs catalyzed HAuCl_(4)/H_(2)O_(2)reaction to enhance SERS signal,and Fe_(3)O_(4)facilitated the magnetic separation and simple detection operations.The SERS aptasensor achieved sensitive detection of STX with a wide linear range from 0.02 to 0.8μmol/L and a low limit of detection(LOD)of 13 nmol/L(signal-to-noise(S/N)=3).To evaluate the performance of this method,STX were detected in the spiked seafood samples with recoveries from 78.75%to 150%with relative standard deviation below 6.52%.Besides,the SERS aptasensor behaved good stability and repeatability,revealing its promising potential in STX detection and food safety detection.
文摘Laguimit Bay is among the few areas in the Philippines where green mussels thrive.However,no studies have been conducted on Harmful Algal Bloom(HAB)in the area.This study investigates the spatial distribution of dinoflagellates associated with HAB in the surface water of the bay.Multiparameter water quality testers were used to determine the physicochemical characteristics while a plankton net was used to filter the dinoflagellates and counting was carried out in a compound microscope.Based on the results,the surface water temperature is between 27–31℃ while the pH ranges from 8.1–8.2 and dissolved oxygen is 6.3–6.5 mg/L at salinity ranges from 30–31 ppt.This indicates that the water quality of the Bay is sustainable for farming mussels.Microscopic examination of the samples showed that the only dinoflagellate associated with HAB is Pyrodinium bahamense var.compressum which causes Paralytic Shellfish Poisoning with an average concentration of 22 cells/L.Other non-HAB species were Protoperidinium depressum with an average of 489 cells/L and the rest are Ceratium macroceros,Ceratium furca,Ceratium trichoceros,Ceratium lunula,Dinophysis caudata,Dinophysis miles.The Shannon-Wiener diversity index was 1.8723 in station 1 and 1.8406 in station 2 and 1.7439 in station 3 which indicates a low diversity index.The presence of P.bahamanse has the potential for recurrence of HAB formation in Laguimit Bay which could affect the economic livelihood of fisherfolk in the country.Continued monitoring and management are recommended to mitigate potential HAB risks and ensure the sustainability of shellfish resources.
