Tissue regeneration requires exogenous and endogenous signals,and there is increasing evidence that the exogenous microenvironment may play an even more dominant role in the complex process of coordinated multiple cel...Tissue regeneration requires exogenous and endogenous signals,and there is increasing evidence that the exogenous microenvironment may play an even more dominant role in the complex process of coordinated multiple cells.The short-distance peripheral nerve showed a spontaneous regenerative phenomenon,which was initiated by the guiding role of macrophages.However,it cannot sufficiently restore long-distance nerve injury by itself.Based on this principle,we firstly constructed a proinflammatory model to prove that abnormal M2 expression reduce the guidance and repair effect of long-distance nerves.Furthermore,a bionic peptide hydrogel scaffold based on self-assembly was developed to envelop M2-derived regenerative cytokines and extracellular vesicles(EVs).The cytokines and EVs were quantified to mimic the guidance and regenerative microenvironment in a direct and mild manner.The bionic scaffold promoted M2 transformation in situ and led to proliferation and migration of Schwann cells,neuron growth and motor function recovery.Meanwhile,the peptide scaffold combined with CX3CL1 recruited more blood-derived M2 macrophages to promote long-distance nerve reconstruction.Overall,we systematically confirmed the important role of M2 in regulating and restoring the injury peripheral nerve.This bionic peptide hydrogel scaffold mimicked and remodeled the local environment for M2 transformation and recruitment,favoring long-distance peripheral nerve regeneration.It can help to explicate regulative effect of M2 may be a cause not just a consequence in nerve repair and tissue integration,which facilitating the development of pro-regenerative biomaterials.展开更多
Bone defects are encountered substantially in clinical practice,and bionic scaffolds represent a promising solution for repairing bone defects.However,it is difficult to fabricate scaffolds with bionic structures and ...Bone defects are encountered substantially in clinical practice,and bionic scaffolds represent a promising solution for repairing bone defects.However,it is difficult to fabricate scaffolds with bionic structures and reconstruct the microenvironment to fulfill the satisfying repair effects.In this review article,we first discuss various strategies for the design and construction of bionic scaffolds to promote bone defect repair,especially including the structural construction of the scaffold and the integration of bioactive substances together with the application of external stimuli.We then discuss the roles of artificial intelligence and medical imaging in aiding clinical treatment.Finally,we point out the challenges and future outlooks in developing multifunctional bone repair scaffolds,aiming to provide insights for improving bone regeneration efficacy and accelerating clinical translation.展开更多
基金the National Natural Science Foundation of China(No.32230057,32271389,31900987)Jiangsu Natural Science Foundation(No.BK20200974)+5 种基金Heilongjiang Natural Science Foundation(No.YQ2019H022)Shuangchuang Program of Jiangsu Province(No.JSSCBS20211603)Nantong Municipal Commission of Health and Family Planning(No.MB2021011)Nantong Science and Technology Plan Project(No.MSZ2022196)Nantong Science and Technology Plan Project(No.JC2019146)Nantong University Clinical Medicine Project(No.2019JZ004).
文摘Tissue regeneration requires exogenous and endogenous signals,and there is increasing evidence that the exogenous microenvironment may play an even more dominant role in the complex process of coordinated multiple cells.The short-distance peripheral nerve showed a spontaneous regenerative phenomenon,which was initiated by the guiding role of macrophages.However,it cannot sufficiently restore long-distance nerve injury by itself.Based on this principle,we firstly constructed a proinflammatory model to prove that abnormal M2 expression reduce the guidance and repair effect of long-distance nerves.Furthermore,a bionic peptide hydrogel scaffold based on self-assembly was developed to envelop M2-derived regenerative cytokines and extracellular vesicles(EVs).The cytokines and EVs were quantified to mimic the guidance and regenerative microenvironment in a direct and mild manner.The bionic scaffold promoted M2 transformation in situ and led to proliferation and migration of Schwann cells,neuron growth and motor function recovery.Meanwhile,the peptide scaffold combined with CX3CL1 recruited more blood-derived M2 macrophages to promote long-distance nerve reconstruction.Overall,we systematically confirmed the important role of M2 in regulating and restoring the injury peripheral nerve.This bionic peptide hydrogel scaffold mimicked and remodeled the local environment for M2 transformation and recruitment,favoring long-distance peripheral nerve regeneration.It can help to explicate regulative effect of M2 may be a cause not just a consequence in nerve repair and tissue integration,which facilitating the development of pro-regenerative biomaterials.
基金State Key Laboratory of Organic-Inorganic Composites,Beijing University of Chemical Technology,Grant/Award Number:oic-202201004National Natural Science Foundation of China,Grant/Award Number:52221006Fundamental Research Funds for the Central Universities,Grant/Award Number:buctrc202312。
文摘Bone defects are encountered substantially in clinical practice,and bionic scaffolds represent a promising solution for repairing bone defects.However,it is difficult to fabricate scaffolds with bionic structures and reconstruct the microenvironment to fulfill the satisfying repair effects.In this review article,we first discuss various strategies for the design and construction of bionic scaffolds to promote bone defect repair,especially including the structural construction of the scaffold and the integration of bioactive substances together with the application of external stimuli.We then discuss the roles of artificial intelligence and medical imaging in aiding clinical treatment.Finally,we point out the challenges and future outlooks in developing multifunctional bone repair scaffolds,aiming to provide insights for improving bone regeneration efficacy and accelerating clinical translation.
