Cell growth kinetics and changes in AFP in nude mice with human hepatoma xenografts were evaluated using the flow cytometry method. After receiving 10 Gy of radiation, the mice showed a marked delay in tumor growth; a...Cell growth kinetics and changes in AFP in nude mice with human hepatoma xenografts were evaluated using the flow cytometry method. After receiving 10 Gy of radiation, the mice showed a marked delay in tumor growth; approximately 1 Gy of radiation caused a tumor growth delay of one day. Irradiation altered various phases of the cell cycle. An acute and temporary block of G2 cells was characteristic; FCM measurements demonstrated that about 58% of cells were blocked in the G2 phase and this blocking effect lasted 90 hours after an irradiation of 10 Gy. This indicated that human hepatoma xenografts in nude mice were quite sensitive to irradiation. It was also noted that the AFP decreased for 96 hours after irradiation. Changes in G2 cells after irradiation may be closely related to changes in AFP.展开更多
A series of new 1-aryloxyacetyl-4-aroylthiosemicarbazides were synthesized by means of solid-liguid phase transfer catalysis. The promoting effects of these new compounds on wheat growth were observed.
Schiff<sup> </sup>base synthesis is usually acid catalyzed and it usually requires refluxing the mixture of aldehydes and amine in ethanolic solution. Synthesis and characterization of Schiff base ligands ...Schiff<sup> </sup>base synthesis is usually acid catalyzed and it usually requires refluxing the mixture of aldehydes and amine in ethanolic solution. Synthesis and characterization of Schiff base ligands derived from substituted amine and salicylaldehyde and their complexes (Cu<sup>2+</sup>, Co<sup>2+</sup>) are reported. The ligands and ligand-complexes were characterized by melting point, FTIR, CHN-elemental analysis and UV-Visible analysis. The UV-Visible and elemental analysis of complexes established (1:2) mole ratio (M:L). The stability constant and thermodynamic parameters (K, ΔG, ΔH, ΔS) were determined at different temperature (30 - 40)°C which established that the metal-complexes were very stable. The review describes the promising biological<sup> </sup>activities of Schiff base and their metal complexes.展开更多
New chelates derived from the novel ligand, (Z)-3-amino-N-(3-amino pyrazine-2-carbonyl)pyrazine-2-carbohydrazonic acid (<strong>APA</strong>, <strong>L</strong>), with Cu<sup>2+</sup&g...New chelates derived from the novel ligand, (Z)-3-amino-N-(3-amino pyrazine-2-carbonyl)pyrazine-2-carbohydrazonic acid (<strong>APA</strong>, <strong>L</strong>), with Cu<sup>2+</sup>, Co<sup>2+</sup>, Pt<sup>4+</sup> and Pd<sup>2+</sup> salts were investigated. The results suggest that APA acts as mononegative tridentate in the case of Cu<sup>2+</sup>, binegative tetradentate in the case of Co<sup>2+</sup> and as mononegative bidentate towards Pt<sup>4+</sup> and Pd<sup>2+</sup> chelates. The results of the corrected <i>μ<sub>eff</sub></i>. and spectral suggest the structures of the isolated chelates. The results of the corrected <i>μ<sub>eff</sub></i>. and spectral suggest the geometries of the isolated chelates. Molecular modeling is deduced and chemical reactivity, energy components for chelates and also MEP for <strong>APA</strong> is illustrated. <i>In Vitro</i>, the SOD and radical scavengers like activity of the synthesized compounds Hep G2 liver cancer cells and cytotoxic activity were checked. Metal chelates show potent anti-oxidative activity. The results of cytotoxic activity assay against hepatocellular carcinoma cell line Hep G2 confirmed that Pt<sup>4+</sup> complex has the highest value, while <strong>APA</strong>, Cu<sup>2+</sup>, Co<sup>2+</sup> and Pd<sup>2+</sup> chelates have no significant cytotoxic activity.展开更多
Signaling peptides are known for their prominent roles in plant growth, development, and environmental adaptation(Zhang et al., 2025). However, their extremely low natural abundance and highly dynamic expression patte...Signaling peptides are known for their prominent roles in plant growth, development, and environmental adaptation(Zhang et al., 2025). However, their extremely low natural abundance and highly dynamic expression patterns pose significant technical challenges to extract sufficient amounts with good purity for biological studies and practical applications.Consequently, chemical synthesis and microbial systems offer attractive alternatives to obtain potent peptides at higher quantities and purity. Incorporating modifications or substitutions, chemically synthetic approaches enable the creation of more effective engineered peptides such as agonists,antagonists, chemically modified peptides, or peptide-like molecules with novel functions compared to native peptides.展开更多
Mn（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ） and Cu（Ⅱ） complexes of 5-mercapto-1,2,4-triazol-3-imine-2＇-hydroxynaphthaline have been synthesized and characterized by elemental analysis, IR, IH NMR, EI-mass, UV-Vis, and ESR （elec...Mn（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ） and Cu（Ⅱ） complexes of 5-mercapto-1,2,4-triazol-3-imine-2＇-hydroxynaphthaline have been synthesized and characterized by elemental analysis, IR, IH NMR, EI-mass, UV-Vis, and ESR （electron spin resonance） spectra, molar conductance, magnetic moment measurements, DC conductivity and thermogravimetric analysis. IR spectra confirm that the ligand molecule existed in both thione and thiole forms. The molar conduc- tance values indicate the complexes are nonelectrolyte. The magnetic moment values of the complexes display paramagnetic behavior. All studies confirm the formation of an octahedral geometry for complex 1 and the other complexes have tetrahedral geometrical structures. The structures of the complexes have also been theoretically studied by using the molecular mechanic calculations by the hyperchem. 8.03 molecular modeling program which confirm the proposed structures. The Schiff-base ligand and its metal complexes have also been screened for their antimicrobial activities.展开更多
The biological effects of relative low level internal irradiation were introduced. 1. Life span studies on carcinogenesis. Pacific Northwest Laboratory observed 3782 rats given a single inhalation of 239Pu O2 at initi...The biological effects of relative low level internal irradiation were introduced. 1. Life span studies on carcinogenesis. Pacific Northwest Laboratory observed 3782 rats given a single inhalation of 239Pu O2 at initial lung burden ranging from 0.25 to 180 nCi. Significant life span shortening was found at lung dose > 8 Gy and it was indicated that the presence of a 'possible' threshold of about 1 Gy for lung tumor formation. 2. Health effects of radon and its progeny. Both experimental and epidemiological survey were studied. The nominal probability coefficient (fatality) for the public and workers are 7.90×105 per mJhm-3 (2.77 ×10~4 per working level month, WLM). 3. Health effects of 3H on postnatal brain development and neurobehavior, genetic effects, carcinogenic effects and adaptive effects of 3H were investigated. 4. Study on the effects of neuroendocrine system under low level irradiation of 75Se (Auger electron emitter) and 35S (β-particles emitter). The results showed that the neuroendocrine system is very sensitive to small dose of internal irradiation.展开更多
Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ...Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ingredients analysis was of necessary to be established for the evaluation of the holistic quality of TCM.As could afford the data with high resolution and high sensitivity,展开更多
A variety of bioorthogonal chemical tools have been developed and widely used in the study of biological phenomena in situ.Tetrazine bioorthogonal chemistry exhibits ultrafast reaction kinetics,excellent biocompatibil...A variety of bioorthogonal chemical tools have been developed and widely used in the study of biological phenomena in situ.Tetrazine bioorthogonal chemistry exhibits ultrafast reaction kinetics,excellent biocompatibility,and precise optical regulatory capabilities.Fluorogenic tetrazine bioorthogonal probes have achieved particularly diverse applications in bioimaging and disease diagnosis and treatment.This Viewpoint briefly introduces the characteristics and advantages of tetrazine bioorthogonal chemistry,some design strategies of fluorogenic tetrazine probes,and the status of applications of these tools to in vivo imaging,as well as disease diagnosis and treatment.Finally,we discuss challenges and propose future trends in the field of fluorogenic tetrazine probes.This Viewpoint offers insights into the development of new bioorthogonal tools for chemical biology research and for the design of new drugs.展开更多
Introduction Artificial intelligence(AI)is a transformative field of study focused on developing computational models that can perform tasks typically requiring human intelligence.Recent breakthroughs in AI have raise...Introduction Artificial intelligence(AI)is a transformative field of study focused on developing computational models that can perform tasks typically requiring human intelligence.Recent breakthroughs in AI have raised optimism about their potential to transform biological research.The study of the structure and function of biological macromolecules such as proteins and RNA represents a key application of AI in biology.展开更多
New chelate compounds,with general formula[M(HL_(1))_(2)](H_(2)O)2,M=Co(Ⅱ),Ni(Ⅱ),Cu(Ⅱ)]and[M(HL_(1))_(2)](H_(2)O),M=Zn(Ⅱ),Cd(Ⅱ),Hg(Ⅱ),were prepared by reacting chlorid salt of these metals with the new azo Schif...New chelate compounds,with general formula[M(HL_(1))_(2)](H_(2)O)2,M=Co(Ⅱ),Ni(Ⅱ),Cu(Ⅱ)]and[M(HL_(1))_(2)](H_(2)O),M=Zn(Ⅱ),Cd(Ⅱ),Hg(Ⅱ),were prepared by reacting chlorid salt of these metals with the new azo Schiff-base ligand 4-((E)-(3-bromo phenyl)diazenyl)-2-((E)-(thiazol-2-ylimino)methylphenol HL1 using the coupling of diazonium salt of 3-bromoaniline with(E)-2-(thiazol-2-ylimino)methyl phenol.The new azo Schiff thiazol ligand and its chelate complexes were characterized by available spectra and analytical methods such as mass spectra,proton nuclear magnetic resonance(1H-NMR),infrared(IR)spectroscopy,ultraviolet-visible spectroscopy(UV-Vis),elemental analysis(CHN),molar conductivity and magnetic susceptibility measurements.Results showed that the ratio of metal:ligand was 1:2,and also showed that Co(Ⅱ),Ni(Ⅱ),and Cu(Ⅱ)chelate complexes had octahedral geometry,while Zn(Ⅱ),Cd(Ⅱ)and Hg(Ⅱ),had tetrahedral geometry.The ligand with its complexes was tested in vintor against the sensitive organisms,including Staphylococcus aureus(gram positive)and Pseudomonas aeruginosa(gram negative).The study showed that the prepared metal complexes had more biological affectivity than the ligands themselves.展开更多
文摘Cell growth kinetics and changes in AFP in nude mice with human hepatoma xenografts were evaluated using the flow cytometry method. After receiving 10 Gy of radiation, the mice showed a marked delay in tumor growth; approximately 1 Gy of radiation caused a tumor growth delay of one day. Irradiation altered various phases of the cell cycle. An acute and temporary block of G2 cells was characteristic; FCM measurements demonstrated that about 58% of cells were blocked in the G2 phase and this blocking effect lasted 90 hours after an irradiation of 10 Gy. This indicated that human hepatoma xenografts in nude mice were quite sensitive to irradiation. It was also noted that the AFP decreased for 96 hours after irradiation. Changes in G2 cells after irradiation may be closely related to changes in AFP.
基金This project was supported by Nature Science Foundation of Gansu.
文摘A series of new 1-aryloxyacetyl-4-aroylthiosemicarbazides were synthesized by means of solid-liguid phase transfer catalysis. The promoting effects of these new compounds on wheat growth were observed.
文摘Schiff<sup> </sup>base synthesis is usually acid catalyzed and it usually requires refluxing the mixture of aldehydes and amine in ethanolic solution. Synthesis and characterization of Schiff base ligands derived from substituted amine and salicylaldehyde and their complexes (Cu<sup>2+</sup>, Co<sup>2+</sup>) are reported. The ligands and ligand-complexes were characterized by melting point, FTIR, CHN-elemental analysis and UV-Visible analysis. The UV-Visible and elemental analysis of complexes established (1:2) mole ratio (M:L). The stability constant and thermodynamic parameters (K, ΔG, ΔH, ΔS) were determined at different temperature (30 - 40)°C which established that the metal-complexes were very stable. The review describes the promising biological<sup> </sup>activities of Schiff base and their metal complexes.
文摘New chelates derived from the novel ligand, (Z)-3-amino-N-(3-amino pyrazine-2-carbonyl)pyrazine-2-carbohydrazonic acid (<strong>APA</strong>, <strong>L</strong>), with Cu<sup>2+</sup>, Co<sup>2+</sup>, Pt<sup>4+</sup> and Pd<sup>2+</sup> salts were investigated. The results suggest that APA acts as mononegative tridentate in the case of Cu<sup>2+</sup>, binegative tetradentate in the case of Co<sup>2+</sup> and as mononegative bidentate towards Pt<sup>4+</sup> and Pd<sup>2+</sup> chelates. The results of the corrected <i>μ<sub>eff</sub></i>. and spectral suggest the structures of the isolated chelates. The results of the corrected <i>μ<sub>eff</sub></i>. and spectral suggest the geometries of the isolated chelates. Molecular modeling is deduced and chemical reactivity, energy components for chelates and also MEP for <strong>APA</strong> is illustrated. <i>In Vitro</i>, the SOD and radical scavengers like activity of the synthesized compounds Hep G2 liver cancer cells and cytotoxic activity were checked. Metal chelates show potent anti-oxidative activity. The results of cytotoxic activity assay against hepatocellular carcinoma cell line Hep G2 confirmed that Pt<sup>4+</sup> complex has the highest value, while <strong>APA</strong>, Cu<sup>2+</sup>, Co<sup>2+</sup> and Pd<sup>2+</sup> chelates have no significant cytotoxic activity.
基金supported by National Natural Science Foundation of China(32370850,32460081)Leading Talent of Technological Innovation of Shaanxi Special Support Program(20249)+3 种基金the Natural Science Foundation of Shaanxi(2020JC-29,2023-JCZD-09)Central Universities Funds(GK202402003)the Jiangxi Agricultural University(9232308314)the Science and Technology Department of Jiangxi Province(20223BCJ25037)。
文摘Signaling peptides are known for their prominent roles in plant growth, development, and environmental adaptation(Zhang et al., 2025). However, their extremely low natural abundance and highly dynamic expression patterns pose significant technical challenges to extract sufficient amounts with good purity for biological studies and practical applications.Consequently, chemical synthesis and microbial systems offer attractive alternatives to obtain potent peptides at higher quantities and purity. Incorporating modifications or substitutions, chemically synthetic approaches enable the creation of more effective engineered peptides such as agonists,antagonists, chemically modified peptides, or peptide-like molecules with novel functions compared to native peptides.
文摘Mn（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ） and Cu（Ⅱ） complexes of 5-mercapto-1,2,4-triazol-3-imine-2＇-hydroxynaphthaline have been synthesized and characterized by elemental analysis, IR, IH NMR, EI-mass, UV-Vis, and ESR （electron spin resonance） spectra, molar conductance, magnetic moment measurements, DC conductivity and thermogravimetric analysis. IR spectra confirm that the ligand molecule existed in both thione and thiole forms. The molar conduc- tance values indicate the complexes are nonelectrolyte. The magnetic moment values of the complexes display paramagnetic behavior. All studies confirm the formation of an octahedral geometry for complex 1 and the other complexes have tetrahedral geometrical structures. The structures of the complexes have also been theoretically studied by using the molecular mechanic calculations by the hyperchem. 8.03 molecular modeling program which confirm the proposed structures. The Schiff-base ligand and its metal complexes have also been screened for their antimicrobial activities.
文摘The biological effects of relative low level internal irradiation were introduced. 1. Life span studies on carcinogenesis. Pacific Northwest Laboratory observed 3782 rats given a single inhalation of 239Pu O2 at initial lung burden ranging from 0.25 to 180 nCi. Significant life span shortening was found at lung dose > 8 Gy and it was indicated that the presence of a 'possible' threshold of about 1 Gy for lung tumor formation. 2. Health effects of radon and its progeny. Both experimental and epidemiological survey were studied. The nominal probability coefficient (fatality) for the public and workers are 7.90×105 per mJhm-3 (2.77 ×10~4 per working level month, WLM). 3. Health effects of 3H on postnatal brain development and neurobehavior, genetic effects, carcinogenic effects and adaptive effects of 3H were investigated. 4. Study on the effects of neuroendocrine system under low level irradiation of 75Se (Auger electron emitter) and 35S (β-particles emitter). The results showed that the neuroendocrine system is very sensitive to small dose of internal irradiation.
文摘Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ingredients analysis was of necessary to be established for the evaluation of the holistic quality of TCM.As could afford the data with high resolution and high sensitivity,
基金support from the National Natural Science Foundation of China(No.22277083)Natural Science Foundation of Sichuan Province(Grant Nos.2023NSFSC53332024NSFTD0023).
文摘A variety of bioorthogonal chemical tools have been developed and widely used in the study of biological phenomena in situ.Tetrazine bioorthogonal chemistry exhibits ultrafast reaction kinetics,excellent biocompatibility,and precise optical regulatory capabilities.Fluorogenic tetrazine bioorthogonal probes have achieved particularly diverse applications in bioimaging and disease diagnosis and treatment.This Viewpoint briefly introduces the characteristics and advantages of tetrazine bioorthogonal chemistry,some design strategies of fluorogenic tetrazine probes,and the status of applications of these tools to in vivo imaging,as well as disease diagnosis and treatment.Finally,we discuss challenges and propose future trends in the field of fluorogenic tetrazine probes.This Viewpoint offers insights into the development of new bioorthogonal tools for chemical biology research and for the design of new drugs.
基金supported by the National Natural Science Foundation of China(82341086,32300521,2422013,32230018,32125007)the National Key Research and Development Project of China(2022YFF1203100)+2 种基金the Open Grant from the Pingyuan Laboratory(2023PY-OP-0104)the State Key Laboratory of Microbial Technology Open Projects Fund(M2023-20),the Intramural Joint Program Fund of the State Key Laboratory of Microbial Technology(SKLMTIJP-2024-02)the Double-First Class Initiative of Shandong University School of Life Sciences,the Young Innovation Team of Shandong Higher Education Institutions,and the Taishan Scholars Youth Expert Program of Shandong Province.
文摘Introduction Artificial intelligence(AI)is a transformative field of study focused on developing computational models that can perform tasks typically requiring human intelligence.Recent breakthroughs in AI have raised optimism about their potential to transform biological research.The study of the structure and function of biological macromolecules such as proteins and RNA represents a key application of AI in biology.
文摘New chelate compounds,with general formula[M(HL_(1))_(2)](H_(2)O)2,M=Co(Ⅱ),Ni(Ⅱ),Cu(Ⅱ)]and[M(HL_(1))_(2)](H_(2)O),M=Zn(Ⅱ),Cd(Ⅱ),Hg(Ⅱ),were prepared by reacting chlorid salt of these metals with the new azo Schiff-base ligand 4-((E)-(3-bromo phenyl)diazenyl)-2-((E)-(thiazol-2-ylimino)methylphenol HL1 using the coupling of diazonium salt of 3-bromoaniline with(E)-2-(thiazol-2-ylimino)methyl phenol.The new azo Schiff thiazol ligand and its chelate complexes were characterized by available spectra and analytical methods such as mass spectra,proton nuclear magnetic resonance(1H-NMR),infrared(IR)spectroscopy,ultraviolet-visible spectroscopy(UV-Vis),elemental analysis(CHN),molar conductivity and magnetic susceptibility measurements.Results showed that the ratio of metal:ligand was 1:2,and also showed that Co(Ⅱ),Ni(Ⅱ),and Cu(Ⅱ)chelate complexes had octahedral geometry,while Zn(Ⅱ),Cd(Ⅱ)and Hg(Ⅱ),had tetrahedral geometry.The ligand with its complexes was tested in vintor against the sensitive organisms,including Staphylococcus aureus(gram positive)and Pseudomonas aeruginosa(gram negative).The study showed that the prepared metal complexes had more biological affectivity than the ligands themselves.
