The rapid development of China's industrial economy makes the environmental engineering wastewater treatment pressure is huge. Compared with other sewage treatment technologies, membrane biological reaction techno...The rapid development of China's industrial economy makes the environmental engineering wastewater treatment pressure is huge. Compared with other sewage treatment technologies, membrane biological reaction technology has better treatment effect, and the effluent quality can be effectively guaranteed. In view of this, this paper mainly analyzes the basic concept of membrane biological reaction technology, and discusses its application in the field of environmental engineering wastewater treatment. Based on this, the space for improving the application of membrane biological reaction technology is also proposed.展开更多
Azole derivatives（3,6） obtained starting from 1-（2-methoxyphenyl） piperazine were converted to the corresponding Mannich bases containing β-lactame or flouroquinolone core via a one pot three component reaction.T...Azole derivatives（3,6） obtained starting from 1-（2-methoxyphenyl） piperazine were converted to the corresponding Mannich bases containing β-lactame or flouroquinolone core via a one pot three component reaction.The synthesis of conazole analogues was carried out starting from triazoles by three steps.Reactions were carried out under conventional and microwave mediated conditions.All the newly synthesized compounds were screened for their antimicrobial,enzyme inhibition and antioxidant activity,and most of them displayed good-moderate activity.Binding affinities and non-covalent interactions between enzyme-ligand complexes were predicted with molecular docking method at molecular level.Docking results complemented well the experimental results on α-glucosidase and urease inhibitory effects of the compounds.Higher binding affinities and much more interaction networks were observed for active compounds in contrary to inactive ones.It was predicted with the docking studies that triazole and anisole moieties in the structure of the synthesized compounds contributed to the stabilization of corresponding enzymes through noncovalent interactions.展开更多
Bacterial biofilms can make traditional antibiotics impenetrable and even promote the development of antibiotic-resistant strains.Therefore,non-antibiotic strategies to effectively penetrate and eradicate the formed b...Bacterial biofilms can make traditional antibiotics impenetrable and even promote the development of antibiotic-resistant strains.Therefore,non-antibiotic strategies to effectively penetrate and eradicate the formed biofilms are urgently needed.Here,we demonstrate the development of selfpropelled biohybrid microrobots that can enhance the degradation and penetration effects for Pseudomonas aeruginosa biofilms in minimally invasive strategy.The biohybrid microrobots(CR@Alg)are constructed by surface modification of Chlamydomonas reinhardtii(CR)microalgae with alginate lyase(Alg)via biological orthogonal reaction.By degrading the biofilm components,the number of CR@Alg microrobots with fast-moving capability penetrating the biofilm increases by around 2.4-fold compared to that of microalgae.Massive reactive oxygen species are subsequently generated under laser irradiation due to the presence of chlorophyll,inherent photosensitizers of microalgae,thus triggering photodynamic therapy(PDT)to combat bacteria.Our algae-based microrobots with superior biocompatibility eliminate biofilm-infections efficiently and tend to suppress the inflammatory response in vivo,showing huge promise for the active treatment of biofilm-associated infections.展开更多
Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferatio...Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferation remains unclear.This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts.CCM samples with different values of surface rouges were prepared by polishing.MC3T3-E1 mouse osteoblasts were used for cell culture experiments.Cell attachment,morphology,and the expression of actin stress fibres,vinculin,and distri-bution of yes-associated protein were analysed.Our results suggest that surface rough-ness does not significantly affect cell attachment and proliferation on CCM,unlike on titanium.Thus implies that other properties of CCM,such as physicochemical properties,may play a more substantial role in modulating cell behaviour.This study provides important insights into the design of CCM implants,suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.展开更多
文摘The rapid development of China's industrial economy makes the environmental engineering wastewater treatment pressure is huge. Compared with other sewage treatment technologies, membrane biological reaction technology has better treatment effect, and the effluent quality can be effectively guaranteed. In view of this, this paper mainly analyzes the basic concept of membrane biological reaction technology, and discusses its application in the field of environmental engineering wastewater treatment. Based on this, the space for improving the application of membrane biological reaction technology is also proposed.
基金provided by Scientific and Technological Research Council of Turkey(TUBITAK,No.113Z181)
文摘Azole derivatives（3,6） obtained starting from 1-（2-methoxyphenyl） piperazine were converted to the corresponding Mannich bases containing β-lactame or flouroquinolone core via a one pot three component reaction.The synthesis of conazole analogues was carried out starting from triazoles by three steps.Reactions were carried out under conventional and microwave mediated conditions.All the newly synthesized compounds were screened for their antimicrobial,enzyme inhibition and antioxidant activity,and most of them displayed good-moderate activity.Binding affinities and non-covalent interactions between enzyme-ligand complexes were predicted with molecular docking method at molecular level.Docking results complemented well the experimental results on α-glucosidase and urease inhibitory effects of the compounds.Higher binding affinities and much more interaction networks were observed for active compounds in contrary to inactive ones.It was predicted with the docking studies that triazole and anisole moieties in the structure of the synthesized compounds contributed to the stabilization of corresponding enzymes through noncovalent interactions.
基金supported by National Key Research and Development Program of China(2022YFA1206900)Guangdong Basic and Applied Basic Research Foundation(2022A1515110659,China)National Natural Science Foundation of China(22175083 and 22375224).
文摘Bacterial biofilms can make traditional antibiotics impenetrable and even promote the development of antibiotic-resistant strains.Therefore,non-antibiotic strategies to effectively penetrate and eradicate the formed biofilms are urgently needed.Here,we demonstrate the development of selfpropelled biohybrid microrobots that can enhance the degradation and penetration effects for Pseudomonas aeruginosa biofilms in minimally invasive strategy.The biohybrid microrobots(CR@Alg)are constructed by surface modification of Chlamydomonas reinhardtii(CR)microalgae with alginate lyase(Alg)via biological orthogonal reaction.By degrading the biofilm components,the number of CR@Alg microrobots with fast-moving capability penetrating the biofilm increases by around 2.4-fold compared to that of microalgae.Massive reactive oxygen species are subsequently generated under laser irradiation due to the presence of chlorophyll,inherent photosensitizers of microalgae,thus triggering photodynamic therapy(PDT)to combat bacteria.Our algae-based microrobots with superior biocompatibility eliminate biofilm-infections efficiently and tend to suppress the inflammatory response in vivo,showing huge promise for the active treatment of biofilm-associated infections.
基金Japan Society for the Promotion of Science,Grant/Award Number:JP19K12798。
文摘Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferation remains unclear.This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts.CCM samples with different values of surface rouges were prepared by polishing.MC3T3-E1 mouse osteoblasts were used for cell culture experiments.Cell attachment,morphology,and the expression of actin stress fibres,vinculin,and distri-bution of yes-associated protein were analysed.Our results suggest that surface rough-ness does not significantly affect cell attachment and proliferation on CCM,unlike on titanium.Thus implies that other properties of CCM,such as physicochemical properties,may play a more substantial role in modulating cell behaviour.This study provides important insights into the design of CCM implants,suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.
