AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transf...AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy,flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay.Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice,and the pathological analysis of tumor formation was performed.Two cytoskeletal proteins were detected by Western blotting. RESULTS:Compared with HepG2 cells,HepG2.2.15 cells showed organelle degeneration and filopodia disappearance under electron microscope.HepG2.2.15 cells proliferated and migrated slowly in vitro,and hardly formed tumor and lung metastasis in nude mice.Flow cytometry showed that the majority of HepG2.2.15 cells were arrested in G1 phase,and apoptosis was minor in both cell lines.Furthermore,the levels of cytoskeletal proteins F-actin and Ezrin were decreased in HepG2.2.15 cells. CONCLUSION:HepG2.2.15 cells demonstrated a lower proliferation and invasion ability than the HepG2 cells due to HBV transfection.展开更多
The aim of this study is to follow each development stage of inflorescence in order to understand the biological feature of flowering and the development of male gametophyte in Anthurium andreanum “Arizona' and ...The aim of this study is to follow each development stage of inflorescence in order to understand the biological feature of flowering and the development of male gametophyte in Anthurium andreanum “Arizona' and to try to find the optimum conditions for its pollination. The methods of dissection and paraffin section were adopted to examine the structural characteristics of anthurium’s tiny floret and the development of the microspore. All the florets of the anthurium arrange on the rhachis helically sub- tended by a colorful bract. Each tiny floret has one gynoecium, four tepals and four stamina. The bract and the florets show different colors during the whole blooming period. The ovary is bicarpellary and has two locules, each of which has one anatropous ovule. The placenta is of a central placentation type. The stylar canal cells not only can produce the secretory mucilage but also can release their own cytoplasm caused by their self-disintegration before the pistil reaches its maturity. The wall of the anther is composed of four layers: epidermis, endothecium, middle layer and tapetum. The tapetal cells and the middle layers’ cells degenerated completely dur- ing meiosis of microsporocytes. The pollen grains were 2-celled at the time of anther dehiscence. Early morning, when the inflores- cences stay at their fifth development stage, is the optimum opportunity for pistil to get pollen grains. The pollen-collection should be done at the end of the seventh stage.展开更多
Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect we...Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect were hereinstudied by playing back its record. The dura-tion of playback was 4 h. Except otherwisestatement, N. lugens tested were virginmacropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5females and 5 males) of N. lugens per cage (4cm in diameter and 8 cm in height). The in-hibitory effect of SMVS was evaluated usingmating rate (i. e. the rate of females withspermatophore). The results were as follows:展开更多
Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigati...Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigating the probable risk factors and establishing comprehensive models for formulating clinical strategies.Methods:Overall,16,158 patients with HCC from 8 hospitals were screened,among whom 3,125 patients who underwent R0 resection were included,and divided into development(n=2,113)and validation(n=1,012)cohorts.A comprehensive study of HPR predictive models and biological features was conducted.Results:Among the 3,125 enrolled patients,506(16.19%)developed HPR.The influence of HPR on extremely poor prognosis was reflected by recurrence features,adverse effects on systemic and liver function,and limited therapeutic options.Nine variables closely associated with HPR were identified,and incorporated into nomogram and conditional inference tree models,which successfully achieved pre-and post-operative HPR risk stratification and facilitated clinical decision-making.Multi-dimensional verification also confirmed the predictive accuracy of model combinations and their reliability in clinical applications.Furthermore,biological analyses revealed that HCCs with HPR exhibited hyperactive biological processes,inactive metabolism,and immune exhaustion features,together with high MYCN/HMGA2 co-expression,thereby enhancing understanding of the molecular events leading to HPR and providing valuable knowledge for HPR management.Conclusions:HPR after hepatectomy is associated with extremely poor prognosis and requires substantial attention.We constructed comprehensive predictive models and propose a clinical strategy for guiding HPR prevention and management.展开更多
Objective To explore the expression of macrophage capping protein(CapG)in colorectal carcinoma tissues,and to investigate its effects on proliferation and migration of colorectal carcinoma cells.Methods From September...Objective To explore the expression of macrophage capping protein(CapG)in colorectal carcinoma tissues,and to investigate its effects on proliferation and migration of colorectal carcinoma cells.Methods From September10th,2015 to March 2nd,2016,the clinical data and tissues specimen of 84 patients with colorectal展开更多
Underwater scene is one of the most marvelous environments in the world. In this study, we present an efficient procedural modeling and rendering system to generate marine ecosystems for swim-through graphic applicati...Underwater scene is one of the most marvelous environments in the world. In this study, we present an efficient procedural modeling and rendering system to generate marine ecosystems for swim-through graphic applications. To produce realistic and natural underwater scenes, several techniques and algorithms have been presented and introduced. First, to distribute sealife naturally on a seabed, we employ an ecosystem simulation that considers the influence of the underwater environment. Second, we propose a two-level procedural modeling system to generate sealife with unique biological features. At the base level, a series of grammars are designed to roughly represent underwater sealife on a central processing unit(CPU). Then at the fine level, additional details of the sealife are created and rendered using graphic processing units(GPUs). Such a hybrid CPU-GPU framework best adopts sequential and parallel computation in modeling a marine ecosystem, and achieves a high level of performance.Third, the proposed system integrates dynamic simulations in the proposed procedural modeling process to support dynamic interactions between sealife and the underwater environment, where interactions and physical factors of the environment are formulated into parameters and control the geometric generation at the fine level. Results demonstrate that this system is capable of generating and rendering scenes with massive corals and sealife in real time.展开更多
基金Supported by Graduate Innovation Foundation of Harbin Medical University No.HCXB2010010Key Technology Project of Heilongjiang Science and Technology Department,No.ZJY04-0102
文摘AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy,flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay.Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice,and the pathological analysis of tumor formation was performed.Two cytoskeletal proteins were detected by Western blotting. RESULTS:Compared with HepG2 cells,HepG2.2.15 cells showed organelle degeneration and filopodia disappearance under electron microscope.HepG2.2.15 cells proliferated and migrated slowly in vitro,and hardly formed tumor and lung metastasis in nude mice.Flow cytometry showed that the majority of HepG2.2.15 cells were arrested in G1 phase,and apoptosis was minor in both cell lines.Furthermore,the levels of cytoskeletal proteins F-actin and Ezrin were decreased in HepG2.2.15 cells. CONCLUSION:HepG2.2.15 cells demonstrated a lower proliferation and invasion ability than the HepG2 cells due to HBV transfection.
基金Supported by the Graduate Students’ Research Foundation of Beijing Forestry University
文摘The aim of this study is to follow each development stage of inflorescence in order to understand the biological feature of flowering and the development of male gametophyte in Anthurium andreanum “Arizona' and to try to find the optimum conditions for its pollination. The methods of dissection and paraffin section were adopted to examine the structural characteristics of anthurium’s tiny floret and the development of the microspore. All the florets of the anthurium arrange on the rhachis helically sub- tended by a colorful bract. Each tiny floret has one gynoecium, four tepals and four stamina. The bract and the florets show different colors during the whole blooming period. The ovary is bicarpellary and has two locules, each of which has one anatropous ovule. The placenta is of a central placentation type. The stylar canal cells not only can produce the secretory mucilage but also can release their own cytoplasm caused by their self-disintegration before the pistil reaches its maturity. The wall of the anther is composed of four layers: epidermis, endothecium, middle layer and tapetum. The tapetal cells and the middle layers’ cells degenerated completely dur- ing meiosis of microsporocytes. The pollen grains were 2-celled at the time of anther dehiscence. Early morning, when the inflores- cences stay at their fifth development stage, is the optimum opportunity for pistil to get pollen grains. The pollen-collection should be done at the end of the seventh stage.
文摘Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect were hereinstudied by playing back its record. The dura-tion of playback was 4 h. Except otherwisestatement, N. lugens tested were virginmacropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5females and 5 males) of N. lugens per cage (4cm in diameter and 8 cm in height). The in-hibitory effect of SMVS was evaluated usingmating rate (i. e. the rate of females withspermatophore). The results were as follows:
基金supported by the National Natural Science Foundation of China(Grant Nos.NSFC 82273405,and 81972306)supported partly by the Guangxi Nature Sciences grants(Grant No.2018GXNSFAA138028)the Guangxi Medical University Training Program for Distinguished Young Scholars.
文摘Objective:Hyper-progression recurrence(HPR)after hepatectomy is a specific recurrence pattern associated with extremely poor prognosis in patients with hepatocellular carcinoma(HCC).This study was aimed at investigating the probable risk factors and establishing comprehensive models for formulating clinical strategies.Methods:Overall,16,158 patients with HCC from 8 hospitals were screened,among whom 3,125 patients who underwent R0 resection were included,and divided into development(n=2,113)and validation(n=1,012)cohorts.A comprehensive study of HPR predictive models and biological features was conducted.Results:Among the 3,125 enrolled patients,506(16.19%)developed HPR.The influence of HPR on extremely poor prognosis was reflected by recurrence features,adverse effects on systemic and liver function,and limited therapeutic options.Nine variables closely associated with HPR were identified,and incorporated into nomogram and conditional inference tree models,which successfully achieved pre-and post-operative HPR risk stratification and facilitated clinical decision-making.Multi-dimensional verification also confirmed the predictive accuracy of model combinations and their reliability in clinical applications.Furthermore,biological analyses revealed that HCCs with HPR exhibited hyperactive biological processes,inactive metabolism,and immune exhaustion features,together with high MYCN/HMGA2 co-expression,thereby enhancing understanding of the molecular events leading to HPR and providing valuable knowledge for HPR management.Conclusions:HPR after hepatectomy is associated with extremely poor prognosis and requires substantial attention.We constructed comprehensive predictive models and propose a clinical strategy for guiding HPR prevention and management.
文摘Objective To explore the expression of macrophage capping protein(CapG)in colorectal carcinoma tissues,and to investigate its effects on proliferation and migration of colorectal carcinoma cells.Methods From September10th,2015 to March 2nd,2016,the clinical data and tissues specimen of 84 patients with colorectal
基金Project supported by the Zhejiang Provincial Natural Science Foundation of China(No.LY13F020002)the National Natural Science Foundation of China(No.61272301)+1 种基金the National Key Technology R&D Program of China(No.2012BAH35B03)the Fundamental Research Funds for the Central Universities,China
文摘Underwater scene is one of the most marvelous environments in the world. In this study, we present an efficient procedural modeling and rendering system to generate marine ecosystems for swim-through graphic applications. To produce realistic and natural underwater scenes, several techniques and algorithms have been presented and introduced. First, to distribute sealife naturally on a seabed, we employ an ecosystem simulation that considers the influence of the underwater environment. Second, we propose a two-level procedural modeling system to generate sealife with unique biological features. At the base level, a series of grammars are designed to roughly represent underwater sealife on a central processing unit(CPU). Then at the fine level, additional details of the sealife are created and rendered using graphic processing units(GPUs). Such a hybrid CPU-GPU framework best adopts sequential and parallel computation in modeling a marine ecosystem, and achieves a high level of performance.Third, the proposed system integrates dynamic simulations in the proposed procedural modeling process to support dynamic interactions between sealife and the underwater environment, where interactions and physical factors of the environment are formulated into parameters and control the geometric generation at the fine level. Results demonstrate that this system is capable of generating and rendering scenes with massive corals and sealife in real time.
