Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR li...Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR light, demonstrating considerable application potential. This study presents the design and synthesis of three nitrogen-doped boron–dipyrrin (Aza-BODIPY) molecules: Aza–BDP–OCH_(3), Aza–BDP–OH, and Aza–BDP-I. Leveraging the strong electron-accepting properties of the Aza-BODIPY core, we developed a donor–acceptor–donor (D-A-D) structure for Aza–BDP–OCH_(3) through modifications with triphenylamine and methoxy groups, resulting in NIR fluorescence. Aza–BDP–OH was obtained via demethylation using boron tribromide, whereas Aza–BDP-I was synthesized by introducing iodine into Aza–BDP–OCH_(3). These three molecules self-assemble with the amphiphilic polymer PMHC18-mPEG to form nanoparticles (NPs), yielding optical nanoprobes. The resulting NPs exhibit NIR emission, good water solubility, and biocompatibility. At a concentration of 100 μg·mL^(-1), these NPs demonstrate low biological toxicity, highlighting their potential for biological applications. Following tail vein injection, Aza–BDP-I NPs accumulate in tumors and effectively illuminate them via the enhanced permeability and retention (EPR) effect. Furthermore, these organic NPs were metabolized by the liver. Therefore, Aza-BODIPY-based NIR fluorescent NPs offer a promising platform for the development of in vivo optical nanoprobes.展开更多
The application of aggregation-induced emission(AIE)materials in biological imaging holds multiple significances,including improving detection sensitivity and specificity,optimizing the imaging process,expanding the s...The application of aggregation-induced emission(AIE)materials in biological imaging holds multiple significances,including improving detection sensitivity and specificity,optimizing the imaging process,expanding the scope of application,and promoting advancements in biomedical research.In this work,the propeller ligand was constructed through McMurry coupling reaction and Suzuki coupling reaction by using dimethoxybenzophenone as the starting material.Then,an imine condensation reaction was carried out in chloroform solution,using a 3:2 molar ratio of precursor to tri(2-aminoethyl)amine to synthesize C3 symmetric porous organic cage CB.The structures of the compounds were determined by nuclear magnetic resonance spectroscopy(NMR),electrospray ionization mass spectrometry(ESI-MS)and Fourier transform infrared spectroscopy(FT-IR).The optical investigation results reveal that ligand L-B and the porous organic cage C_(B) demonstrate remarkable aggregation-induced emission(AIE)properties in a tetrahydrofuran/water mixed solvent system,along with a pronounced response to tetrahydrofuran vapor stimuli.Consequently,Furthermore,given its unique cage-like structure,high quantum yield,and outstanding AIE behavior,the porous organic cage C_(B) holds promise for applications in cell imaging.展开更多
Donor-acceptor(D-A)compounds are particularly important in optoelectronic and biological applications.However,they are normally synthesized in the presence of transition metal catalysts.Herein,we report a metal-free m...Donor-acceptor(D-A)compounds are particularly important in optoelectronic and biological applications.However,they are normally synthesized in the presence of transition metal catalysts.Herein,we report a metal-free method by a complexmediated nucleophilic aromatic substitution of aryl nitriles with amines.The method can lead to rich D-A type aggregation-induced emission luminogens(AIEgens)with tunable properties.They emit from deep-blue to yellow-green and possess high photoluminescence quantum yields up to 70.5%in the aggregate state.Interestingly,the suppression of intramolecular flapping is proved to play an indispensable role in the AIE behavior,which is different from the mechanism met in other AIEgens.Moreover,the biocompatible AIEgens possess specific staining of lipid droplets in HeLa cells and the superiority of identifying fatty liver over traditional Oil Red O staining is exhibited.展开更多
DNA nanomaterials hold great promise in biomedical fields due to its excellent sequence programmability,molecular recognition ability and biocompatibility.Hybridization chain reaction(HCR)is a simple and efficient iso...DNA nanomaterials hold great promise in biomedical fields due to its excellent sequence programmability,molecular recognition ability and biocompatibility.Hybridization chain reaction(HCR)is a simple and efficient isothermal enzyme-free amplification strategy of DNA,generating nicked double helices with repeated units.Through the design of HCR hairpins,multiple nanomaterials with desired functions are assembled by DNA,exhibiting great potential in biomedical applications.Herein,the recent progress of HCR-based DNA nanomaterials for biosensing,bioimaging and therapeutics are summarized.Representative works are exemplified to demonstrate how HCR-based DNA nanomaterials are designed and constructed.The challenges and prospects of the development of HCR-based DNA nanomaterials are discussed.We envision that rationally designing HCR-based DNA nanomaterials will facilitate the development of biomedical applications.展开更多
As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an inno...As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT,which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group,respectively.TCF-CHT exhibited excellent selectivity and eye-catching sensitivity(8.91 ng/m L)towards CHT,“off-on”long-wavelength emission at 670 nm and large Stokes shift(140 nm).Furthermore,the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms(P815 cells,HepG2 cells,zebrafish and tumor-bearing mice)verified its potential as a powerful tool for the recognition of CHT in complicated biological environments.展开更多
The development of large-scale synthetic methods for high quality carbon quantum dots(CQDs) is fundamental to their applications.However,the macroscopic preparation and scale up synthetic of CQDs is still in its infan...The development of large-scale synthetic methods for high quality carbon quantum dots(CQDs) is fundamental to their applications.However,the macroscopic preparation and scale up synthetic of CQDs is still in its infancy.Here,we report a facile,green,kilogram-scale synthesis of high quality fluorescent CQDs derived from poplar leaves via a one-step hydrothermal method.Notably,the throughput of CQDs can reach a level up to as high as 1.4975 kg in one pot.The structure and properties of the as-prepared CQDs were assessed through TEM,XRD,XPS and various spectroscopic methods.The obtained high quality CQD s with a photoluminescent quantum yield of 10.64% showed remarkable stability in aqueous media,rich functional groups,high photostability,consistent photoluminescence within biological pH range and low cytotoxicity.On account of these good properties,we demonstrated the multifunctional application to electrocatalytic water splitting,Fe^3+ sensing and bioimaging.It showed remarkable electrocatalytic activity,Fe^3+ sensitivity and good biocompatibility.This study provides a green,facile,inexpensive and large-scale method for producing high quality CQDs,which provides application value for large-scale production of CQDs.展开更多
Persistent luminescence nanoparticles (PLNPs) are a series of emerging luminescent nanomaterials which can emit persistently after ceasing the external excitation. Due to the long decay time of persistent luminescence...Persistent luminescence nanoparticles (PLNPs) are a series of emerging luminescent nanomaterials which can emit persistently after ceasing the external excitation. Due to the long decay time of persistent luminescence, the background autofluorescence in complex sample and tissues can be effectively eliminated, thus significantly improving the sensitivity of bioanalysis. Besides, such a long decay time of luminescence also makes PLNPs valuable for long-term bioimaging. Benefiting from these merits, PLNPs have been widely used for biomedical applications, especially biosensing and bioimaging. In this review,we conclude the progress in the application of PLNPs at biosensing and bioimaging in recent years, and also provide our understanding of the prospects.展开更多
Peptide self-assembled nanomaterials have attracted more and more attention due to their wide applications such as drug delive ry,cell imaging,and real-time drug monitoring.However,the application of the peptide is st...Peptide self-assembled nanomaterials have attracted more and more attention due to their wide applications such as drug delive ry,cell imaging,and real-time drug monitoring.However,the application of the peptide is still limited by its inherent optical properties.Here we proposed and prepared a series of fluorescent tripeptide nanoparticles(TPNPs)through π-π stacking and zinc coordination.The experimental results show that the nanoparticles(TPNPs1)formed by the self-assembly of the tripeptide tryptophan-tryptophan-tryptophan have the highest fluorescence intensity,uniform and appropriate size,and low cytotoxicity.Furthermore,there was fluorescence resonance between TPNPs1 and doxorubicin,which has been successfully applied for real-time cell imaging and drug release monitoring.展开更多
Combining treatment and diagnosis,called theranostics,which is achieved within single nanoparticle is an ultimate goal of many studies.Herein,we developed a new nanotheranostic agent-Nd^(3+)-sensitized upconversion na...Combining treatment and diagnosis,called theranostics,which is achieved within single nanoparticle is an ultimate goal of many studies.Herein,we developed a new nanotheranostic agent-Nd^(3+)-sensitized upconversion nanoparticles core for dual modal imaging(i.e.,upconversion luminescence imaging and magnetic resonance imaging) and antimony nanoshell for photothermal therapy(PTT).The core-shellshell upconversion nanoparticles(NaYF_(4):Yb,Er@NaYF_(4):Yb,Nd@NaGdF_(4):Nd,named as UCNP) were firstly synthesized using thermal decomposition method and then were coated by antimony shell over the surface of UCNP using simple cost and time effective new method.Furthermore,the surface of UCNP@Sb nanostructures was modified with DSPE-PEG in order to enhance the water solubility and biocompatibility.The final nanotheranostic agent,named as UCNP@Sb-PEG,exhibits very low toxicity,good biocompatibility,very good photothermal therapeutic effect,and efficient upconversion luminescence(UCL) imaging of HeLa cells under only one laser(808 nm) irradiation.The antimony shell is quenching the upconversion emission in pristine nanotheranostic agent,but interestingly,the UCL intensity of the agent recovers progressively under 808 nm laser irradiation due to light induced degradability of antimony shell.Besides,high longitudinal relaxivity(r_(1)) obtained from the experiment approves excellent potential of the nanotheranostic agent for T_(1)-weighted magnetic resonance imaging application.展开更多
Developing the novel fluorescent dyes with a larger Stokes shift is still a challenge in the research of fluorescence probes. In this work, a naphthalimide-modified near-infrared cyanine dye with an emission at 785 nm...Developing the novel fluorescent dyes with a larger Stokes shift is still a challenge in the research of fluorescence probes. In this work, a naphthalimide-modified near-infrared cyanine dye with an emission at 785 nm has been synthesized for lysosome-targeting imaging. This fluorescent dye showed a large Stokes shift（up to 165 nm） and favorable lysosome-targeting property, which facilitated it to be a potential candidate for studying of lysosomal functions. The result also indicated that the probe is a promising contrast agent for in vivo imaging in mouse models.展开更多
Alkaline-earth dihalide nanocrystals(NCs) such as SrFCl, owing to their high chemical stability and low phonon energy, are excellent host materials for lanthanide(Ln3+) doping to achieve desirable optical properties f...Alkaline-earth dihalide nanocrystals(NCs) such as SrFCl, owing to their high chemical stability and low phonon energy, are excellent host materials for lanthanide(Ln3+) doping to achieve desirable optical properties for various bioapplications, Herein, we report a novel strategy for the synthesis of sub-10 nm Ln3+-doped SrFCl NCs with efficient upconverting and downshifting luminescence through a facile onestep hot-injection method. Utilizing the temperature-dependent upconverting luminescence(UCL) from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, we showed the potential of SrFCl:Yb,Er NCs as sensitive UCL nanoprobes for non-contact thermal sensing with a maximum detection sensitivity of 0.0066 K-1, which is among the highest values for thermal sensing based on Er3+-activated UCL nanoprobes. Furthermore, by employing the intense downshifting luminescence from Tb3+ and Eu3+, we demonstrated the successful use of biotinylated SrFCl:Ce,Tb and SrFCl:Eu3+ nanoprobes for biotin receptor-targeted cancer cell imaging, thus revealing the great promise of SrFCl:Ln3+ nanoprobes for versatile bioapplications.展开更多
A pyridoxal-based chemosensor was synthesized by reacting hydrazine hydrate and pyridoxal hydrochloride in ethanol and characterized by NMR and ESI-MS. The optical properties of the compound were investigated in a met...A pyridoxal-based chemosensor was synthesized by reacting hydrazine hydrate and pyridoxal hydrochloride in ethanol and characterized by NMR and ESI-MS. The optical properties of the compound were investigated in a methanol： HEPES solution. The compound displayed selectivity for Cu2＋, as evidenced by a colorless to yellow color change, which was characterized using UV-vis spectroscopy. The fluorescence of the compound can be quenched only by Cu2＋, accompanying by a color change from blue to colorless. Furthermore, it can be used in bioimaging.展开更多
The biocompatibility and biodegradability of peptide self-assembled materials makes them suitable for many biological applications,such as targeted drug delivery,bioimaging,and tracking of therapeutic agents.According...The biocompatibility and biodegradability of peptide self-assembled materials makes them suitable for many biological applications,such as targeted drug delivery,bioimaging,and tracking of therapeutic agents.According to our previous research,self-assembled fluorescent peptide nanoparticles can overcome the intrinsic optical properties of peptides.However,monochromatic fluorescent nanomaterials have many limitations as luminescent agents in biomedical applications.Therefore,combining different fluorescent species into one nanostructure to prepare fluorescent nanoparticles with multiple emission wavelengths has become a very attractive research area in the bioimaging field.In this study,the tetrapeptide Trp-Trp-Trp-Trp(WWWW)was self-assembled into multicolor fluorescent nanoparticles(TPNPs).The results have demonstrated that TPNPs have the blue,green,red and near infrared(NIR)fluorescence emission wavelength.Moreover,TPNPs have shown excellent performance in multicolor bioimaging,biocompatibility,and photostability.The facile preparation and multicolor fluorescence features make TPNPs potentially useful in multiplex bioanalysis and diagnostics.展开更多
Considering that hydrogen peroxide(H2O2)plays significant roles in oxidative stress,the cellular signal transduction and essential biological process regulation,the detection and imaging of H2O2 in living systems unde...Considering that hydrogen peroxide(H2O2)plays significant roles in oxidative stress,the cellular signal transduction and essential biological process regulation,the detection and imaging of H2O2 in living systems undertakes critical responsibility.Herein,we have developed a novel two-photon fluorescence turn on probe,named as Pyp-B for mitochondria H2O2 detection in living systems.Selectivity studies show that probe Pyp-B exhibit highly sensitive response toward H2O2 than other reactive oxygen species(ROS)and reactive nitrogen species(RNS)as well as biologically relevant species.The fluorescence colocalization studies demonstrate that the probe can localize in the mitochondria solely.Furthermore,as a bio-compatibility molecule,the highly selective and sensitive of fluorescence probe Pyp-B have been confirmed by its cell imaging application of H2O2 in living A549 cells and zebrafishes under the physiological conditions.展开更多
A fluorescence-enhanced probe PY, which is based on integration of pyrene moiety and amino acid group,was developed for the orthogonal recognition of In3＋ and Al3＋ by the quick formation of 1：1 complex. PY shows hi...A fluorescence-enhanced probe PY, which is based on integration of pyrene moiety and amino acid group,was developed for the orthogonal recognition of In3＋ and Al3＋ by the quick formation of 1：1 complex. PY shows high selectivity and sensitivity for In3＋ in NaOAc/HOAc（pH 4.8） buffer solution but rather for Al3＋ in methanol. Moreover, this probe shows good cell permeability, and the recognition of In3＋ by PY was successfully applied in bioimaging, which was the first example for detection of this metal ion in living cells.展开更多
An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The stru...An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The structure of the compound was solved by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21, with a = 9.753(5), b = 8.815(5), c = 25.554(5) ?, β = 96.315(5)°, V = 2184(2) ?~3, Z = 2, D_c = 1.136 g/m^3, F(000) = 792, Μr = 746.92, μ = 0.069 mm^(-1), the final R = 0.0658 and wR = 0.1730 for 6790 observed reflections with I > 2(I). Study of nonlinear optical properties shows that the compound exhibits excellent two-photon excited fluorescence with the two-photon absorption cross-section value of 116 GM. The structure-property relationship was researched in detail through X-ray crystallography and quantum chemical calculation. Result of living cell imaging experiment shows its potential in fluorescence microscopy bioimaging.展开更多
Promising biomedical applications of hybrid materials composed of gold nanoparticles and nucleic acids have attracted strong interest from the nanobiotechnological community.The particular interest is owing to the rob...Promising biomedical applications of hybrid materials composed of gold nanoparticles and nucleic acids have attracted strong interest from the nanobiotechnological community.The particular interest is owing to the robust and easy-to-make synthetic approaches,to the versatile optical and catalytic properties of gold nanoparticles combined with the molecular recognition and programmable properties of nucleic acids.The significant progress is made in the develop-ment of DNA-gold nanostructures and their applications,such as molecular recognition,cell and tissue bioimaging,targeted delivery of therapeutic agents,etc.This review is focused on the critical discussion of the recent applications of the gold nanoparticles-nucleic acids hybrids.The effect of particle size,surface,charge and thermal properties on the interactions with functional nucleic acids is discussed.For each of the above topics,the basic principles,recent advances,and current challenges are discussed.Emphasis is placed on the systematization of data over the theranostic systems on the basis of the gold nanoparticles-nucleic acids hybrids.Specifically,we start our discussion with observation of the recent data on interaction of various gold nano-particles with nucleic acids.Further we describe existing gene delivery systems,nucleic acids detection,and bioimaging technologies.Finally,we describe the phenomenon of the polymerase chain reaction improvement by gold nanoparticle additives and its potential underlying mechanisms.Lastly,we provide a short summary of reported data and outline the challenges and perspectives.展开更多
Early diagnosis and fast detection with a high accuracy rate of lung cancer are important to improve the treatment effect.In this research,an early fast diagnosis and in vivo imaging method for lung adenocarcinoma are...Early diagnosis and fast detection with a high accuracy rate of lung cancer are important to improve the treatment effect.In this research,an early fast diagnosis and in vivo imaging method for lung adenocarcinoma are proposed by collecting the spectral data from normal and patients'cells/tissues,such as Fourier infrared spectroscopy(FTIR),UV-vis absorbance,and fluorescence spectra using anthocyanin.The FTIR spectra of human normal lung epithelial cells(BEAS-2B cells)and human lung adenocarcinoma cells(A549 cells)were collected.After the data is cleaned,a feature selection algorithm is used to select important wavelengths,and then,the classification models of support vector machine(SVM)and the grid search method are used to select the optimal model parameters(accuracy:96.89%on the training set and 88.57%on the test set).The optimal model is used to classify all samples,and the accuracy is 94.37%.Moreover,the anthocyanin was prepared and used for the intracellular absorbance and fluorescence,and the optimized algorithm was used for classification(accuracy:91.38%on the training set and 80.77%on the test set).Most importantly,the in vivo cancer imaging can be performed using anthocyanin.The results show that there are differences between lung ade-nocarcinoma and normal lung tissues at the molecular level,reflecting the accuracy,intui-tiveness,and feasibility of this algorithm-assistant anthocyanin imaging in lung cancer diagnosis,thus showing the potential to become an accurate and effective technical means for basic research and clinical diagnosis.展开更多
CRISPR/Cas system has been utilized to rationally manipulate intracellular genes,and it has been engineered as versatile and efficient gene editing tools with precise site-specificity and excellent targeting ability f...CRISPR/Cas system has been utilized to rationally manipulate intracellular genes,and it has been engineered as versatile and efficient gene editing tools with precise site-specificity and excellent targeting ability for therapeutics,diagnostics,and bioimaging.Here,the evolution and application of CRISPR/Cas systems were sketched chronologically.Landmark works were exemplified to illustrate the design principles of CRISPR/Cas systems.Furthermore,the delivery vectors of CRISPR/Cas system especially DNA nanomaterials-based vectors were categorized and illuminated.DNA nanomaterials are suitable for CRISPR/Cas system delivery via base pairing due to its sequence programmability and biocompatibility.Then the applications of CRISPR/Cas in diagnosis and genomic imaging were highlighted.At the end of the review,the challenges and opportunities of CRISPR/Cas systems were deeply discussed.We envision that the grant advances on CRISPR/Cas systems will promote the development of interdisciplinary fields in chemistry,biology and medicine.展开更多
The in vivo degradation behavior of metallic nanoparticles(NPs) is very important for their biomedical applications and safety evaluation.Here,a method of laser ablation-single particle inductively coupled plasma mass...The in vivo degradation behavior of metallic nanoparticles(NPs) is very important for their biomedical applications and safety evaluation.Here,a method of laser ablation-single particle inductively coupled plasma mass spectrometry(LA-sp-ICP-MS) is shown to have high spatial resolution,sensitivity and accuracy for simultaneous imaging the in situ distribution of particulate Ag(P-Ag) and released ionic Ag(IonAg) in the sub-organs of spleen,liver and kidney after intravenous injection of Ag nanoparticles(50 nm,AgNPs) to mice.Under the optimized parameters of 0.4 J/cm^(2) laser fluence on a 30 μm spot with dwell time at 100 μs,the signals of P-Ag and Ion-Ag in the organic tissues can be easily distinguished from the mass spectra.The method of iterative threshold algorithm has been used to distract the signals of P-Ag and Ion-Ag and separate each other.The resulting images for the first time provide visualized evidence that a considerable amount of P-Ag accumulated in the splenic marginal zone,but widely distributed in the liver parenchyma at 24 h after injection of AgNPs,and in the meantime,obvious amounts of ionic Ag released and distributed in the organs.In addition,the imaging results indicate that the AgNP excretion in the kidney is mainly in ionic forms.The investigation here demonstrates that the developed LA-sp-ICPMS method with high spatial resolution,sensitivity and visualization capability can become a powerful tool in the clinical context of metallic NPs.展开更多
基金supported by the National Key R&D Program of China (2023YFA0913600)the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX23-1480)H. Liao and Q. Meng contributed equally to this work.
文摘Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR light, demonstrating considerable application potential. This study presents the design and synthesis of three nitrogen-doped boron–dipyrrin (Aza-BODIPY) molecules: Aza–BDP–OCH_(3), Aza–BDP–OH, and Aza–BDP-I. Leveraging the strong electron-accepting properties of the Aza-BODIPY core, we developed a donor–acceptor–donor (D-A-D) structure for Aza–BDP–OCH_(3) through modifications with triphenylamine and methoxy groups, resulting in NIR fluorescence. Aza–BDP–OH was obtained via demethylation using boron tribromide, whereas Aza–BDP-I was synthesized by introducing iodine into Aza–BDP–OCH_(3). These three molecules self-assemble with the amphiphilic polymer PMHC18-mPEG to form nanoparticles (NPs), yielding optical nanoprobes. The resulting NPs exhibit NIR emission, good water solubility, and biocompatibility. At a concentration of 100 μg·mL^(-1), these NPs demonstrate low biological toxicity, highlighting their potential for biological applications. Following tail vein injection, Aza–BDP-I NPs accumulate in tumors and effectively illuminate them via the enhanced permeability and retention (EPR) effect. Furthermore, these organic NPs were metabolized by the liver. Therefore, Aza-BODIPY-based NIR fluorescent NPs offer a promising platform for the development of in vivo optical nanoprobes.
基金funded by the National Natural Science Foundation of China(Nos.22101267 and 82103686)the Natural Science Foundation of Henan Province(202300410477 and 24230042123)the China Postdoctoral Science Foundation(Nos.2021M692905 and 2024T170832).
文摘The application of aggregation-induced emission(AIE)materials in biological imaging holds multiple significances,including improving detection sensitivity and specificity,optimizing the imaging process,expanding the scope of application,and promoting advancements in biomedical research.In this work,the propeller ligand was constructed through McMurry coupling reaction and Suzuki coupling reaction by using dimethoxybenzophenone as the starting material.Then,an imine condensation reaction was carried out in chloroform solution,using a 3:2 molar ratio of precursor to tri(2-aminoethyl)amine to synthesize C3 symmetric porous organic cage CB.The structures of the compounds were determined by nuclear magnetic resonance spectroscopy(NMR),electrospray ionization mass spectrometry(ESI-MS)and Fourier transform infrared spectroscopy(FT-IR).The optical investigation results reveal that ligand L-B and the porous organic cage C_(B) demonstrate remarkable aggregation-induced emission(AIE)properties in a tetrahydrofuran/water mixed solvent system,along with a pronounced response to tetrahydrofuran vapor stimuli.Consequently,Furthermore,given its unique cage-like structure,high quantum yield,and outstanding AIE behavior,the porous organic cage C_(B) holds promise for applications in cell imaging.
基金supported by the National Natural Science Foundation of China(22275072 and 62105184)the Natural Science Foundation of Guangdong Province(2020A1515010622)+1 种基金the Project of Science and Technology of Guangzhou(2024A04J3712)the Teli Young Scholar Program of Beijing Institute of Technology.
文摘Donor-acceptor(D-A)compounds are particularly important in optoelectronic and biological applications.However,they are normally synthesized in the presence of transition metal catalysts.Herein,we report a metal-free method by a complexmediated nucleophilic aromatic substitution of aryl nitriles with amines.The method can lead to rich D-A type aggregation-induced emission luminogens(AIEgens)with tunable properties.They emit from deep-blue to yellow-green and possess high photoluminescence quantum yields up to 70.5%in the aggregate state.Interestingly,the suppression of intramolecular flapping is proved to play an indispensable role in the AIE behavior,which is different from the mechanism met in other AIEgens.Moreover,the biocompatible AIEgens possess specific staining of lipid droplets in HeLa cells and the superiority of identifying fatty liver over traditional Oil Red O staining is exhibited.
基金supported in part by National Natural Science Foundation of China(Nos.22225505,22174097).
文摘DNA nanomaterials hold great promise in biomedical fields due to its excellent sequence programmability,molecular recognition ability and biocompatibility.Hybridization chain reaction(HCR)is a simple and efficient isothermal enzyme-free amplification strategy of DNA,generating nicked double helices with repeated units.Through the design of HCR hairpins,multiple nanomaterials with desired functions are assembled by DNA,exhibiting great potential in biomedical applications.Herein,the recent progress of HCR-based DNA nanomaterials for biosensing,bioimaging and therapeutics are summarized.Representative works are exemplified to demonstrate how HCR-based DNA nanomaterials are designed and constructed.The challenges and prospects of the development of HCR-based DNA nanomaterials are discussed.We envision that rationally designing HCR-based DNA nanomaterials will facilitate the development of biomedical applications.
基金financial support provided by National Natural Science Foundation of China(Nos.21775005,41430641 and 41140032)the Start-Up Fund of Qingdao University of Science and Technology(No.12030430010883)。
文摘As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT,which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group,respectively.TCF-CHT exhibited excellent selectivity and eye-catching sensitivity(8.91 ng/m L)towards CHT,“off-on”long-wavelength emission at 670 nm and large Stokes shift(140 nm).Furthermore,the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms(P815 cells,HepG2 cells,zebrafish and tumor-bearing mice)verified its potential as a powerful tool for the recognition of CHT in complicated biological environments.
基金financial support from Project funded by China postdoctoral Science Foundation (No. 2018M640681)
文摘The development of large-scale synthetic methods for high quality carbon quantum dots(CQDs) is fundamental to their applications.However,the macroscopic preparation and scale up synthetic of CQDs is still in its infancy.Here,we report a facile,green,kilogram-scale synthesis of high quality fluorescent CQDs derived from poplar leaves via a one-step hydrothermal method.Notably,the throughput of CQDs can reach a level up to as high as 1.4975 kg in one pot.The structure and properties of the as-prepared CQDs were assessed through TEM,XRD,XPS and various spectroscopic methods.The obtained high quality CQD s with a photoluminescent quantum yield of 10.64% showed remarkable stability in aqueous media,rich functional groups,high photostability,consistent photoluminescence within biological pH range and low cytotoxicity.On account of these good properties,we demonstrated the multifunctional application to electrocatalytic water splitting,Fe^3+ sensing and bioimaging.It showed remarkable electrocatalytic activity,Fe^3+ sensitivity and good biocompatibility.This study provides a green,facile,inexpensive and large-scale method for producing high quality CQDs,which provides application value for large-scale production of CQDs.
基金supported by the National Natural Science Foundation of China (No. 21675120)the National Key R&D Program of China (Nos. 2017YFA0208000, 2016YFF0100800)+2 种基金the National Postdoctoral Program for Innovative Talents (No. BX20180223)the National Basic Research Program of China (973 Program, No. 2015CB932600)the Ten Thousand Talents Program for Young Talents
文摘Persistent luminescence nanoparticles (PLNPs) are a series of emerging luminescent nanomaterials which can emit persistently after ceasing the external excitation. Due to the long decay time of persistent luminescence, the background autofluorescence in complex sample and tissues can be effectively eliminated, thus significantly improving the sensitivity of bioanalysis. Besides, such a long decay time of luminescence also makes PLNPs valuable for long-term bioimaging. Benefiting from these merits, PLNPs have been widely used for biomedical applications, especially biosensing and bioimaging. In this review,we conclude the progress in the application of PLNPs at biosensing and bioimaging in recent years, and also provide our understanding of the prospects.
基金the National Natural Science Foundation of China(Nos.31900984 and 31971315)the Natural Science Basic Research Plan in Shaanxi Province of China(No.2019JQ-231)+2 种基金the China Postdoctoral Science Foundation(No.2018M631197)the Shaanxi Postdoctoral Science Foundation(No.2018BSHQYXMZZ42)the Fundamental Research Funds for the Central Universities(No.31020180QD063)。
文摘Peptide self-assembled nanomaterials have attracted more and more attention due to their wide applications such as drug delive ry,cell imaging,and real-time drug monitoring.However,the application of the peptide is still limited by its inherent optical properties.Here we proposed and prepared a series of fluorescent tripeptide nanoparticles(TPNPs)through π-π stacking and zinc coordination.The experimental results show that the nanoparticles(TPNPs1)formed by the self-assembly of the tripeptide tryptophan-tryptophan-tryptophan have the highest fluorescence intensity,uniform and appropriate size,and low cytotoxicity.Furthermore,there was fluorescence resonance between TPNPs1 and doxorubicin,which has been successfully applied for real-time cell imaging and drug release monitoring.
基金Project supported by the National Natural Science Foundation of China(51872183)"Shuguang Program"supported by Shanghai Education Development Foundation+1 种基金Shanghai Municipal Education Commission(19SG38)the National Basic Research Program of China(2016YFA0201600)。
文摘Combining treatment and diagnosis,called theranostics,which is achieved within single nanoparticle is an ultimate goal of many studies.Herein,we developed a new nanotheranostic agent-Nd^(3+)-sensitized upconversion nanoparticles core for dual modal imaging(i.e.,upconversion luminescence imaging and magnetic resonance imaging) and antimony nanoshell for photothermal therapy(PTT).The core-shellshell upconversion nanoparticles(NaYF_(4):Yb,Er@NaYF_(4):Yb,Nd@NaGdF_(4):Nd,named as UCNP) were firstly synthesized using thermal decomposition method and then were coated by antimony shell over the surface of UCNP using simple cost and time effective new method.Furthermore,the surface of UCNP@Sb nanostructures was modified with DSPE-PEG in order to enhance the water solubility and biocompatibility.The final nanotheranostic agent,named as UCNP@Sb-PEG,exhibits very low toxicity,good biocompatibility,very good photothermal therapeutic effect,and efficient upconversion luminescence(UCL) imaging of HeLa cells under only one laser(808 nm) irradiation.The antimony shell is quenching the upconversion emission in pristine nanotheranostic agent,but interestingly,the UCL intensity of the agent recovers progressively under 808 nm laser irradiation due to light induced degradability of antimony shell.Besides,high longitudinal relaxivity(r_(1)) obtained from the experiment approves excellent potential of the nanotheranostic agent for T_(1)-weighted magnetic resonance imaging application.
基金support from National Natural Science Foundation of China (Nos. 21676113, 21402057, 21472059, 81671803)Youth Chen-Guang Project of Wuhan (2016070204010098)+2 种基金the 111 Project B17019the Ministry Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, Shenzhensupported by Self-determined Research Funds of CCNU from the colleges’ basic research and operation of MOE (No. CCNU16A02004)
文摘Developing the novel fluorescent dyes with a larger Stokes shift is still a challenge in the research of fluorescence probes. In this work, a naphthalimide-modified near-infrared cyanine dye with an emission at 785 nm has been synthesized for lysosome-targeting imaging. This fluorescent dye showed a large Stokes shift（up to 165 nm） and favorable lysosome-targeting property, which facilitated it to be a potential candidate for studying of lysosomal functions. The result also indicated that the probe is a promising contrast agent for in vivo imaging in mouse models.
基金Project supported by the CAS/SAFEA International Partnership Program for Creative Research Teamsthe National Natural Science Foundation of China(21771185,11774345,21875250,21650110462)+2 种基金the CAS Youth Innovation Promotion Association(2016277)the Chunmiao Project of Haixi Institutes of the CAS(CMZX-2016-002)Natural Science Foundation of Fujian Province(201710018)
文摘Alkaline-earth dihalide nanocrystals(NCs) such as SrFCl, owing to their high chemical stability and low phonon energy, are excellent host materials for lanthanide(Ln3+) doping to achieve desirable optical properties for various bioapplications, Herein, we report a novel strategy for the synthesis of sub-10 nm Ln3+-doped SrFCl NCs with efficient upconverting and downshifting luminescence through a facile onestep hot-injection method. Utilizing the temperature-dependent upconverting luminescence(UCL) from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, we showed the potential of SrFCl:Yb,Er NCs as sensitive UCL nanoprobes for non-contact thermal sensing with a maximum detection sensitivity of 0.0066 K-1, which is among the highest values for thermal sensing based on Er3+-activated UCL nanoprobes. Furthermore, by employing the intense downshifting luminescence from Tb3+ and Eu3+, we demonstrated the successful use of biotinylated SrFCl:Ce,Tb and SrFCl:Eu3+ nanoprobes for biotin receptor-targeted cancer cell imaging, thus revealing the great promise of SrFCl:Ln3+ nanoprobes for versatile bioapplications.
基金supported by the National Natural Science Foundation of China (No.21102086)the Shanxi Province Science Foundation for Youths (Nos.2012021009-4 and 2013011011-1)+4 种基金the Shanxi Province Foundation for Returnee (No.2012-007)the Taiyuan Technology Star Special (No.12024703)the Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi (TYMIT,No.2013802)talents Support Program of Shanxi Province (No.2014401)CAS Key Laboratory of Analytical Chemistry for Living Biosystems Open Foundation (No.ACL201304)
文摘A pyridoxal-based chemosensor was synthesized by reacting hydrazine hydrate and pyridoxal hydrochloride in ethanol and characterized by NMR and ESI-MS. The optical properties of the compound were investigated in a methanol： HEPES solution. The compound displayed selectivity for Cu2＋, as evidenced by a colorless to yellow color change, which was characterized using UV-vis spectroscopy. The fluorescence of the compound can be quenched only by Cu2＋, accompanying by a color change from blue to colorless. Furthermore, it can be used in bioimaging.
基金supported by the National Natural Science Foundation of China(No.31900984)the Fundamental Research Funds for the Central Universities(No.D5000210899)Innovation and Entrepreneurship Fund from the Student Affairs Department of the Party Committee of Northwestern Polytechnic University(No.2021-CXCY-019)。
文摘The biocompatibility and biodegradability of peptide self-assembled materials makes them suitable for many biological applications,such as targeted drug delivery,bioimaging,and tracking of therapeutic agents.According to our previous research,self-assembled fluorescent peptide nanoparticles can overcome the intrinsic optical properties of peptides.However,monochromatic fluorescent nanomaterials have many limitations as luminescent agents in biomedical applications.Therefore,combining different fluorescent species into one nanostructure to prepare fluorescent nanoparticles with multiple emission wavelengths has become a very attractive research area in the bioimaging field.In this study,the tetrapeptide Trp-Trp-Trp-Trp(WWWW)was self-assembled into multicolor fluorescent nanoparticles(TPNPs).The results have demonstrated that TPNPs have the blue,green,red and near infrared(NIR)fluorescence emission wavelength.Moreover,TPNPs have shown excellent performance in multicolor bioimaging,biocompatibility,and photostability.The facile preparation and multicolor fluorescence features make TPNPs potentially useful in multiplex bioanalysis and diagnostics.
基金the financial support from the National Natural Science Foundation of China(No.81860630)the China Postdoctoral Science Foundation(No.2019M662968)GuangdongBasic and Applied Basic Research Foundation(Nos.2019A1515110356,2019A1515110877)。
文摘Considering that hydrogen peroxide(H2O2)plays significant roles in oxidative stress,the cellular signal transduction and essential biological process regulation,the detection and imaging of H2O2 in living systems undertakes critical responsibility.Herein,we have developed a novel two-photon fluorescence turn on probe,named as Pyp-B for mitochondria H2O2 detection in living systems.Selectivity studies show that probe Pyp-B exhibit highly sensitive response toward H2O2 than other reactive oxygen species(ROS)and reactive nitrogen species(RNS)as well as biologically relevant species.The fluorescence colocalization studies demonstrate that the probe can localize in the mitochondria solely.Furthermore,as a bio-compatibility molecule,the highly selective and sensitive of fluorescence probe Pyp-B have been confirmed by its cell imaging application of H2O2 in living A549 cells and zebrafishes under the physiological conditions.
基金supported by the National Natural Science Foundation of China (Nos. 21572091 and 21472075)the Fundamental Research Funds for the Central Universities (No. lzujbky-2016-51) by MoE of China
文摘A fluorescence-enhanced probe PY, which is based on integration of pyrene moiety and amino acid group,was developed for the orthogonal recognition of In3＋ and Al3＋ by the quick formation of 1：1 complex. PY shows high selectivity and sensitivity for In3＋ in NaOAc/HOAc（pH 4.8） buffer solution but rather for Al3＋ in methanol. Moreover, this probe shows good cell permeability, and the recognition of In3＋ by PY was successfully applied in bioimaging, which was the first example for detection of this metal ion in living cells.
基金supported by the Major Project of Natural Science Research in Universities of Anhui Province(KJ2018ZD037,KJ2018A0333)Key Project of Youth Talents in Universities of Anhui Province(gxyqZD2017067)+5 种基金National Natural Science Foundation of China(21401024)Natural Science Foundation of Anhui Province(1508085MB21)National Students Research Training Program(201810371028)Research Innovation Team of Fuyang Normal University(kytd201710)Horizontal Cooperation Project of Fuyang Municipal Government and Fuyang Normal University(XDHX2016011,XDHX2016004)Anhui University Research Innovation Platform Team Project(201549)
文摘An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The structure of the compound was solved by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21, with a = 9.753(5), b = 8.815(5), c = 25.554(5) ?, β = 96.315(5)°, V = 2184(2) ?~3, Z = 2, D_c = 1.136 g/m^3, F(000) = 792, Μr = 746.92, μ = 0.069 mm^(-1), the final R = 0.0658 and wR = 0.1730 for 6790 observed reflections with I > 2(I). Study of nonlinear optical properties shows that the compound exhibits excellent two-photon excited fluorescence with the two-photon absorption cross-section value of 116 GM. The structure-property relationship was researched in detail through X-ray crystallography and quantum chemical calculation. Result of living cell imaging experiment shows its potential in fluorescence microscopy bioimaging.
基金The work by P.T.E.was supported by the Saratov State Medical University according to the research project No SSMU-2021-001The part of the work(observation of SERS-based strategies)was supported by a grant from the Russian Science Foundation no.18-14-00016-Ⅱ.
文摘Promising biomedical applications of hybrid materials composed of gold nanoparticles and nucleic acids have attracted strong interest from the nanobiotechnological community.The particular interest is owing to the robust and easy-to-make synthetic approaches,to the versatile optical and catalytic properties of gold nanoparticles combined with the molecular recognition and programmable properties of nucleic acids.The significant progress is made in the develop-ment of DNA-gold nanostructures and their applications,such as molecular recognition,cell and tissue bioimaging,targeted delivery of therapeutic agents,etc.This review is focused on the critical discussion of the recent applications of the gold nanoparticles-nucleic acids hybrids.The effect of particle size,surface,charge and thermal properties on the interactions with functional nucleic acids is discussed.For each of the above topics,the basic principles,recent advances,and current challenges are discussed.Emphasis is placed on the systematization of data over the theranostic systems on the basis of the gold nanoparticles-nucleic acids hybrids.Specifically,we start our discussion with observation of the recent data on interaction of various gold nano-particles with nucleic acids.Further we describe existing gene delivery systems,nucleic acids detection,and bioimaging technologies.Finally,we describe the phenomenon of the polymerase chain reaction improvement by gold nanoparticle additives and its potential underlying mechanisms.Lastly,we provide a short summary of reported data and outline the challenges and perspectives.
基金This work was supported by the National Key R&D Program of China Grant(Nos.2018YFC0910602,2017YFA0205202,and 2017YFC1309100)the Natural Science Foundation of China(NSFC 81801744)the Fundamental Research Funds for the Central Universities.
文摘Early diagnosis and fast detection with a high accuracy rate of lung cancer are important to improve the treatment effect.In this research,an early fast diagnosis and in vivo imaging method for lung adenocarcinoma are proposed by collecting the spectral data from normal and patients'cells/tissues,such as Fourier infrared spectroscopy(FTIR),UV-vis absorbance,and fluorescence spectra using anthocyanin.The FTIR spectra of human normal lung epithelial cells(BEAS-2B cells)and human lung adenocarcinoma cells(A549 cells)were collected.After the data is cleaned,a feature selection algorithm is used to select important wavelengths,and then,the classification models of support vector machine(SVM)and the grid search method are used to select the optimal model parameters(accuracy:96.89%on the training set and 88.57%on the test set).The optimal model is used to classify all samples,and the accuracy is 94.37%.Moreover,the anthocyanin was prepared and used for the intracellular absorbance and fluorescence,and the optimized algorithm was used for classification(accuracy:91.38%on the training set and 80.77%on the test set).Most importantly,the in vivo cancer imaging can be performed using anthocyanin.The results show that there are differences between lung ade-nocarcinoma and normal lung tissues at the molecular level,reflecting the accuracy,intui-tiveness,and feasibility of this algorithm-assistant anthocyanin imaging in lung cancer diagnosis,thus showing the potential to become an accurate and effective technical means for basic research and clinical diagnosis.
基金supported in part by National Natural Science Foundation of China(Nos.22225505,21621004,21905196 and 31971305)Tianjin Natural Science Foundation(Basic Research Plan,No.18JCJQJC47600)National Key R&D Program of China(Nos.2018YFA0902300,2019YFA0905800)。
文摘CRISPR/Cas system has been utilized to rationally manipulate intracellular genes,and it has been engineered as versatile and efficient gene editing tools with precise site-specificity and excellent targeting ability for therapeutics,diagnostics,and bioimaging.Here,the evolution and application of CRISPR/Cas systems were sketched chronologically.Landmark works were exemplified to illustrate the design principles of CRISPR/Cas systems.Furthermore,the delivery vectors of CRISPR/Cas system especially DNA nanomaterials-based vectors were categorized and illuminated.DNA nanomaterials are suitable for CRISPR/Cas system delivery via base pairing due to its sequence programmability and biocompatibility.Then the applications of CRISPR/Cas in diagnosis and genomic imaging were highlighted.At the end of the review,the challenges and opportunities of CRISPR/Cas systems were deeply discussed.We envision that the grant advances on CRISPR/Cas systems will promote the development of interdisciplinary fields in chemistry,biology and medicine.
基金supported by the National Natural Science Foundation of China (Nos. 11975251, 11875268)。
文摘The in vivo degradation behavior of metallic nanoparticles(NPs) is very important for their biomedical applications and safety evaluation.Here,a method of laser ablation-single particle inductively coupled plasma mass spectrometry(LA-sp-ICP-MS) is shown to have high spatial resolution,sensitivity and accuracy for simultaneous imaging the in situ distribution of particulate Ag(P-Ag) and released ionic Ag(IonAg) in the sub-organs of spleen,liver and kidney after intravenous injection of Ag nanoparticles(50 nm,AgNPs) to mice.Under the optimized parameters of 0.4 J/cm^(2) laser fluence on a 30 μm spot with dwell time at 100 μs,the signals of P-Ag and Ion-Ag in the organic tissues can be easily distinguished from the mass spectra.The method of iterative threshold algorithm has been used to distract the signals of P-Ag and Ion-Ag and separate each other.The resulting images for the first time provide visualized evidence that a considerable amount of P-Ag accumulated in the splenic marginal zone,but widely distributed in the liver parenchyma at 24 h after injection of AgNPs,and in the meantime,obvious amounts of ionic Ag released and distributed in the organs.In addition,the imaging results indicate that the AgNP excretion in the kidney is mainly in ionic forms.The investigation here demonstrates that the developed LA-sp-ICPMS method with high spatial resolution,sensitivity and visualization capability can become a powerful tool in the clinical context of metallic NPs.
