Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）,...Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.展开更多
Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear fac...Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear factor （erythroid-derived 2）-like 2/Kelch-like ECH-associated protein 1 （Nrf2/Keap1） system plays a major role in maintaining the balance between the production and elimination of ROS via the regulation of a series of detoxifying and antioxidant enzyme gene expressions by means of the antioxidant response element （ARE）. Dietary phytochemicals, which are generally found in vegetables, fruits, grains, and herbs, have been reported to have health benefits and to improve the growth performance and meat quality of farm animals through the regulation of Nrf2-mediated phase II enzymes in a variety of ways. However, the enormous quantity of somewhat chaotic data that is available on the effects of phytochemicals needs to be properly classified according to the functions or mechanisms of phytochemicals, in this review, we first introduce the antioxidant properties of phytochemicals and their relation to the Nrf2/Keap1 system. We then summarize the effects of phytochemicals on the growth performance, meat quality, and intestinal microbiota of farm animals via targeting the Nrf2/Keap1 system. These exhaustive data contribute to better illuminate the underlying biofunctional properties of phytochemicals in farm animals.展开更多
Calcium phosphate(CaP)has been widely used for bone defect repair due to good biocompatibility and osteoconductivity.Additive manufacture of calcium phosphate bioceramics with tailored architectures and improved mecha...Calcium phosphate(CaP)has been widely used for bone defect repair due to good biocompatibility and osteoconductivity.Additive manufacture of calcium phosphate bioceramics with tailored architectures and improved mechanical properties has recently attracted great attention.Herein,calcium phosphate nanoparticles with the size of~89-164 nm were synthesized by the hydrothermal treatment of amorphous calcium phosphate(ACP)precursors at 180°C for 24 h.Biofunctional elements including Mg,Sr and Zn have been doped into these calcium phosphate nanoparticles.Our results revealed that Mg^(2+)ions played critical roles in formation of whitlockite-type calcium phosphate(not hydroxyapatite)from ACP precursors.Moreover,gyroid scaffolds with bionic triply periodic minimal surface structures were fabricated using stereolithography printing of these calcium phosphate nanoparticles,which are likely used as biofunctional scaffolds for bone repair.展开更多
Mg alloys,as a new generation of biodegradable bone implant materials,are facing two tremendous challenges of enhancing strength and reducing degradation rate in physiological environment to meet clinical needs.In thi...Mg alloys,as a new generation of biodegradable bone implant materials,are facing two tremendous challenges of enhancing strength and reducing degradation rate in physiological environment to meet clinical needs.In this study,tricalcium phosphate(β-TCP)particles were dispersed in Mg–2 Zn–0.46 Y–0.5 Nd alloy by friction stir processing(FSP)to produce Mg-based functional gradient materials(Mg/β-TCP FGM).On the surface of Mg/β-TCP FGM,the hydroxyapatite(HA)coating was prepared by electrodeposition.The effects of FSP and electrochemical parameter on the microstructure,microhardness,bonding strength and corrosion performance of the Mg/β-TCP FGM were investigated.After four passes of FSP,a uniform and fine-grained structure was formed in Mg/β-TCP and the microhardness increased from 47.9 to 76.3 HV.Compared to the samples withoutβ-TCP,the bonding strength of the Mg/β-TCP FGM increased from 23.1±0.462 to 26.3±0.526 MPa and the addition of degradableβ-TCP contributed to the in situ growth of HA coating.The thickness of HA coating could be dominated by controlling the parameters of electrodeposition.According to the results of immersion tests and electrochemical tests in simulated body fluid,it indicated that the degradation rate of the Mg/β-TCP FGM could be adjusted.展开更多
Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to ...Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.展开更多
In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibilit...In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibility to implant stainless steel having high strength and toughness.It has been Known from the experimental results that the adhesion of DLC coated to implant stainless steel have been significantly improved with the gradient interlayers,and biofuntionally gradient materials having excellent haemocompatibilty,strength and toughness have been prepared successively.展开更多
Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of m...Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance biofunctions.Calcium phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration properties.As such,they have been widely used in clinical orthopedics and dentistry.The new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from CaPs.This review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP coatings.We first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,i.e.osteointegration,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials.展开更多
Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,ef...Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,effective bone regeneration in the necrotic area is still a significant challenge.This study developed a biofunctionalized composite scaffold(PLGA/nHA30VEGF)for osteonecrosis therapy through potentiation of osteoconduction,angiogenesis,and a favorable metabolic microenvironment.The composite scaffold had a porosity of 87.7%and compressive strength of 8.9 MPa.PLGA/nHA30VEGF had an average pore size of 227.6μm and a water contact angle of 56.5◦with a sustained release profile of vascular endothelial growth factor(VEGF).After the implantation of PLGA/nHA30VEGF,various osteogenic and angiogenic biomarkers were upregulated by 2-9 fold compared with no treatment.Additionally,the metabolomic and lipidomic profiling studies demonstrated that PLGA/nHA30VEGF effectively regulated the multiple metabolites and more than 20 inordinate metabolic pathways in osteonecrosis.The excellent performances reveal that the biofunctionalized composite scaffold provides an advanced adjuvant therapy modality for osteonecrosis.展开更多
The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modificatio...The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are required.However,the functions of conventional bioactive coatings are limited.Thus novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue repair.By physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and silicon.It was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy implants.In addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are promising.Mg coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial effects.Meanwhile,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating ability.Mg coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in vivo.Inhibitory effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic patients.In addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg coatings.Thus,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties.These novel multi-functional Mg coatings are expected to significantly enhance the long-term safety of bone implants for the benefit of patients.This paper gives a brief review of studies of the microstructure,degradation behaviours and biofunctions of Mg coatings,and directions for future research are also proposed.展开更多
1 Results We have attempted to conjugate inorganic nanoparticles with biofunctional molecules.Recently we were quite successful in demonstrating that a two-dimensional inorganic compound like layered double hydroxide ...1 Results We have attempted to conjugate inorganic nanoparticles with biofunctional molecules.Recently we were quite successful in demonstrating that a two-dimensional inorganic compound like layered double hydroxide (LDH),and natural and synthetic clays can be used as gene or drug delivery carriers1-4.To the best of our knowledge,such inorganic vectors are completely new and different from conventionally developed ones such as viruses and cationic liposomes,those which are limited in certain cases of ap...展开更多
Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted prod...Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted products(BPP)based on co-products(canola or carinata)from processing with different proportions of pulse pea screenings and lignosulfonate chemical compound.Method:The protein molecular structures were determined using the non-invasive advanced vibrational molecular spectroscopy(ATR-FT/IR)in terms of chemical structure and biofunctional groups of amides(ⅠandⅡ),α-helix andβ-sheet.Results:The results showed that increasing the level of the co-products in BPP significantly increased the spectral intensity of the amide area and amide height.The products exhibited similar protein secondaryα-helix toβ-sheet ratio.The protein molecular structure profiles(amidesⅠandⅡ,α-helix toβ-sheet)were highly associated with protein degradation kinetics and intestinal digestion.In conclusion,the non-invasive vibrational molecular spectroscopy(ATR-FT/IR)could be used to detect inherent structural make-up characteristics in BPP.Conclusion:The molecular structural features related to protein biopolymer were highly associated with protein utilization and metabolism.展开更多
Inspired by the skin structure,an asymmetric wettability tri-layer nanofiber membrane(TNM)consisting of hydrophilic inner layer loaded with lidocaine hydrochloride(LID),hydrophobic middle layer with ciprofloxacin(CIP)...Inspired by the skin structure,an asymmetric wettability tri-layer nanofiber membrane(TNM)consisting of hydrophilic inner layer loaded with lidocaine hydrochloride(LID),hydrophobic middle layer with ciprofloxacin(CIP)and hydrophobic outer layer has been created.The hydrophobic outer layer endows the TNM with waterproof function and anti-adhesion from contaminants.The hydrophobic middle layer with CIP preserves long-term inhibition of bacteria growth and the hydrophilic inner layer with LID possesses optimal waterabsorbing capacity and air permeability.The TNM dramatically elevates the water contact angles from 10°(inner layer)to 120(outer layer),indicating an asymmetric wettability,which could directionally transport wound exudate within the materials and meanwhile maintain a comfortable and moist environment to promote wound healing.Furthermore,the sequential release of LID and CIP could relieve pain rapidly and achieve antibacterial effect in the long run,respectively.In addition,the TNM shows superior biocompatibility towards L929 cells.The in vivo results show the TNM could prevent infection,accelerate epithelial regeneration and significantly accelerate wound healing.This study indicates the developed TNM with asymmetrical wettability and synergetic drug release shows great potential as a wound dressing in clinical application.展开更多
基金supported by NIH R01 DE14190(HX),R21 DE22625(HX)and R01 HL-073781(LC)the University of Maryland School of Dentistry startup fund(HX)
文摘Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.
基金This work was financially supported by funds from the Core Research Program 1515 of Hunan Agricultural University, the National Natural Science Foundation of China （31101268）, and Scholar Research of Kagoshima University of Japan （for De-Xing Hou）.
文摘Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear factor （erythroid-derived 2）-like 2/Kelch-like ECH-associated protein 1 （Nrf2/Keap1） system plays a major role in maintaining the balance between the production and elimination of ROS via the regulation of a series of detoxifying and antioxidant enzyme gene expressions by means of the antioxidant response element （ARE）. Dietary phytochemicals, which are generally found in vegetables, fruits, grains, and herbs, have been reported to have health benefits and to improve the growth performance and meat quality of farm animals through the regulation of Nrf2-mediated phase II enzymes in a variety of ways. However, the enormous quantity of somewhat chaotic data that is available on the effects of phytochemicals needs to be properly classified according to the functions or mechanisms of phytochemicals, in this review, we first introduce the antioxidant properties of phytochemicals and their relation to the Nrf2/Keap1 system. We then summarize the effects of phytochemicals on the growth performance, meat quality, and intestinal microbiota of farm animals via targeting the Nrf2/Keap1 system. These exhaustive data contribute to better illuminate the underlying biofunctional properties of phytochemicals in farm animals.
基金financially supported by the National Key Research and Development Program of China from Ministry of Science and Technology(No.2016YFC1100502)the Doctoral Research Foundation Program of Liaoning Province(No.2019-BS-256)+2 种基金the Key Research Program of Frontier Sciences(No.QYZDY-SSWJSC031)from Chinese Academy of Sciences(CAS)Key Research and Development Program of Liaoning Province(No.201703031)Shenyang Key R&D and Technology Transfer Program(No.Z17-7023)。
文摘Calcium phosphate(CaP)has been widely used for bone defect repair due to good biocompatibility and osteoconductivity.Additive manufacture of calcium phosphate bioceramics with tailored architectures and improved mechanical properties has recently attracted great attention.Herein,calcium phosphate nanoparticles with the size of~89-164 nm were synthesized by the hydrothermal treatment of amorphous calcium phosphate(ACP)precursors at 180°C for 24 h.Biofunctional elements including Mg,Sr and Zn have been doped into these calcium phosphate nanoparticles.Our results revealed that Mg^(2+)ions played critical roles in formation of whitlockite-type calcium phosphate(not hydroxyapatite)from ACP precursors.Moreover,gyroid scaffolds with bionic triply periodic minimal surface structures were fabricated using stereolithography printing of these calcium phosphate nanoparticles,which are likely used as biofunctional scaffolds for bone repair.
基金financial support of the National Natural Science Foundation of China(No.U1804251)the National Key Research and Development Program of China(Nos.2018YFC1106703 and 2017YFB0702504).
文摘Mg alloys,as a new generation of biodegradable bone implant materials,are facing two tremendous challenges of enhancing strength and reducing degradation rate in physiological environment to meet clinical needs.In this study,tricalcium phosphate(β-TCP)particles were dispersed in Mg–2 Zn–0.46 Y–0.5 Nd alloy by friction stir processing(FSP)to produce Mg-based functional gradient materials(Mg/β-TCP FGM).On the surface of Mg/β-TCP FGM,the hydroxyapatite(HA)coating was prepared by electrodeposition.The effects of FSP and electrochemical parameter on the microstructure,microhardness,bonding strength and corrosion performance of the Mg/β-TCP FGM were investigated.After four passes of FSP,a uniform and fine-grained structure was formed in Mg/β-TCP and the microhardness increased from 47.9 to 76.3 HV.Compared to the samples withoutβ-TCP,the bonding strength of the Mg/β-TCP FGM increased from 23.1±0.462 to 26.3±0.526 MPa and the addition of degradableβ-TCP contributed to the in situ growth of HA coating.The thickness of HA coating could be dominated by controlling the parameters of electrodeposition.According to the results of immersion tests and electrochemical tests in simulated body fluid,it indicated that the degradation rate of the Mg/β-TCP FGM could be adjusted.
基金supported by National Key Research and Development Program of China(2021YFA0910900)the National Natural Science Foundation of China(32222044,22104147)+5 种基金Shenzhen Municipal Science and Technology Innovation Council(RCYX20210609103823046)Youth Innovation Promotion Association CAS(2021359)Natural Science Foundation of Guangdong(2020A1515111130)Guangdong Provincial Key Laboratory of Synthetic Genomics(2019B030301006)Shenzhen Science and Technology Program(KQTD20180413181837372)Shenzhen Outstanding Talents Training Fund.
文摘Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.
基金The project is supported by Natural Science Foundation of China
文摘In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibility to implant stainless steel having high strength and toughness.It has been Known from the experimental results that the adhesion of DLC coated to implant stainless steel have been significantly improved with the gradient interlayers,and biofuntionally gradient materials having excellent haemocompatibilty,strength and toughness have been prepared successively.
基金supported by National Institutes of Health[Grant number R01HL140562]。
文摘Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance biofunctions.Calcium phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration properties.As such,they have been widely used in clinical orthopedics and dentistry.The new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from CaPs.This review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP coatings.We first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,i.e.osteointegration,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials.
基金This study was financially supported by the National Natural Science Foundation of China(Grant Nos.52022095,51973216,51873207,51803006,and 51833010)the Provincial Health Specific Project of Jilin Province(Grant Nos.JLSWSRCZX2020-0021,2018SCZ018,and SCZSY201710)+3 种基金the Specific Project for Health Research Talents of Jilin Province(Grant No.2019SCZ025)the Science and Technology Development Program of Jilin Province(Grant Nos.20200404182YY,20200201478JC,and 20190303154SF)the Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2019230)and the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing(Wuhan University of Technology)(Grant No.2020-KF-5).In addition,the authors are grateful to Wuhan Metware Biotechnology Co.,Ltd,Wuhan,P.R.China,for the assistance in metabolomic analysis and Dr.Di Li from the First Hospital of Jilin University,P.R.China,for the valuable discussion.
文摘Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,effective bone regeneration in the necrotic area is still a significant challenge.This study developed a biofunctionalized composite scaffold(PLGA/nHA30VEGF)for osteonecrosis therapy through potentiation of osteoconduction,angiogenesis,and a favorable metabolic microenvironment.The composite scaffold had a porosity of 87.7%and compressive strength of 8.9 MPa.PLGA/nHA30VEGF had an average pore size of 227.6μm and a water contact angle of 56.5◦with a sustained release profile of vascular endothelial growth factor(VEGF).After the implantation of PLGA/nHA30VEGF,various osteogenic and angiogenic biomarkers were upregulated by 2-9 fold compared with no treatment.Additionally,the metabolomic and lipidomic profiling studies demonstrated that PLGA/nHA30VEGF effectively regulated the multiple metabolites and more than 20 inordinate metabolic pathways in osteonecrosis.The excellent performances reveal that the biofunctionalized composite scaffold provides an advanced adjuvant therapy modality for osteonecrosis.
基金supported by the National Key Research and Development Program of China(Nos.2016YFC1101804,2016YFC1100604)National Natural Science Foundation of China(Nos.51971222,51631009)+2 种基金Natural Science Foundation of Liaoning Province of China(No.2019-MS-326)Dongguan Innovative Research Team Program of China(No.2020607234007)China Postdoctoral Science Foundation(No.2021M690494).
文摘The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are required.However,the functions of conventional bioactive coatings are limited.Thus novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue repair.By physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and silicon.It was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy implants.In addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are promising.Mg coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial effects.Meanwhile,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating ability.Mg coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in vivo.Inhibitory effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic patients.In addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg coatings.Thus,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties.These novel multi-functional Mg coatings are expected to significantly enhance the long-term safety of bone implants for the benefit of patients.This paper gives a brief review of studies of the microstructure,degradation behaviours and biofunctions of Mg coatings,and directions for future research are also proposed.
文摘1 Results We have attempted to conjugate inorganic nanoparticles with biofunctional molecules.Recently we were quite successful in demonstrating that a two-dimensional inorganic compound like layered double hydroxide (LDH),and natural and synthetic clays can be used as gene or drug delivery carriers1-4.To the best of our knowledge,such inorganic vectors are completely new and different from conventionally developed ones such as viruses and cationic liposomes,those which are limited in certain cases of ap...
基金financially supported by the grants from Sask Pulse Growers,Natural Sciences and Engineering Research Council of Canada(NSERC)the Sask Canola,the Ministry of Agriculture Strategic Research Chair ProgramSask Milk.
文摘Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted products(BPP)based on co-products(canola or carinata)from processing with different proportions of pulse pea screenings and lignosulfonate chemical compound.Method:The protein molecular structures were determined using the non-invasive advanced vibrational molecular spectroscopy(ATR-FT/IR)in terms of chemical structure and biofunctional groups of amides(ⅠandⅡ),α-helix andβ-sheet.Results:The results showed that increasing the level of the co-products in BPP significantly increased the spectral intensity of the amide area and amide height.The products exhibited similar protein secondaryα-helix toβ-sheet ratio.The protein molecular structure profiles(amidesⅠandⅡ,α-helix toβ-sheet)were highly associated with protein degradation kinetics and intestinal digestion.In conclusion,the non-invasive vibrational molecular spectroscopy(ATR-FT/IR)could be used to detect inherent structural make-up characteristics in BPP.Conclusion:The molecular structural features related to protein biopolymer were highly associated with protein utilization and metabolism.
文摘Inspired by the skin structure,an asymmetric wettability tri-layer nanofiber membrane(TNM)consisting of hydrophilic inner layer loaded with lidocaine hydrochloride(LID),hydrophobic middle layer with ciprofloxacin(CIP)and hydrophobic outer layer has been created.The hydrophobic outer layer endows the TNM with waterproof function and anti-adhesion from contaminants.The hydrophobic middle layer with CIP preserves long-term inhibition of bacteria growth and the hydrophilic inner layer with LID possesses optimal waterabsorbing capacity and air permeability.The TNM dramatically elevates the water contact angles from 10°(inner layer)to 120(outer layer),indicating an asymmetric wettability,which could directionally transport wound exudate within the materials and meanwhile maintain a comfortable and moist environment to promote wound healing.Furthermore,the sequential release of LID and CIP could relieve pain rapidly and achieve antibacterial effect in the long run,respectively.In addition,the TNM shows superior biocompatibility towards L929 cells.The in vivo results show the TNM could prevent infection,accelerate epithelial regeneration and significantly accelerate wound healing.This study indicates the developed TNM with asymmetrical wettability and synergetic drug release shows great potential as a wound dressing in clinical application.
