Biofl oc technology(BFT)improves water quality,and productivity of the farmed species through converting ammonium nitrogen to microbial protein,stabilizing microbial community,and reducing the production cost.In this ...Biofl oc technology(BFT)improves water quality,and productivity of the farmed species through converting ammonium nitrogen to microbial protein,stabilizing microbial community,and reducing the production cost.In this study,a small-scale biofl oc development unit was designed in combination of recirculation system(RAS)for Artemia culture.Artemia growth,water quality,and microbial composition of biofl ocs in RAS were studied in comparison with in-situ batch culture(Glu).Glucose was added in RAS and Glu at C/N ratio of 10.The cultures without glucose addition,but with 50%daily water renewal(WRe)and without water renewal(NWRe)were considered as the controls.Arte mia were cultured at 25℃ and salinity 30 for 24 days and fed formulated feed.The results showed that compared to the controls,Glu signifi cantly improved the Artemia biomass,increased the biofl oc volume,and reduced the content of total ammonia nitrogen(TAN),nitrite nitrogen(NO_(2)-N)and nitrate nitrogen(NO_(3)-N)in water column(P<0.05).In addition,RAS had similar results with Glu.High throughput sequencing analysis on biofl oc microbial composition demonstrated that glucose supplement shaped the microbial community structure,and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth.Furthermore,we analyzed the relationship between the microbial composition of biofl oc and environmental factors.Canonical correspondence analysis(CCA)indicated that inorganic nitrogen in culture water had great impact on biofl oc microbial composition in NWRe and WRe,whilst the dissolved organic carbon(DOC)modifi ed the microbial community in Glu and RAS.This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.展开更多
The growth of aquaculture demands intense consumption of formulated foods,scarce natural resources such as water and land.The increase in aquaculture production needs to be sustainable in several aspects of the activi...The growth of aquaculture demands intense consumption of formulated foods,scarce natural resources such as water and land.The increase in aquaculture production needs to be sustainable in several aspects of the activity,including the use of more sustainable farming systems that provide reduced water demand,less space dependence for increased production,and availability of complementary natural food.In this perspective,the biofloc system(BFT),presents itself as a more friendly cultivation technology as a way to mitigate some impacts of aquaculture production.Much research has been carried out,addressing different aspects important to the production of fish in BFT.In this review,we address how stocking density,carbon sources and carbon nitrogen ration(C/N)affect animal performance and the nutritional value of bioflocs;the use of microbial aggregates as“in situ”and“ex situ”food and the benefits in feed conversion,and their influence on the immune system and disease resistance.展开更多
Controlling toxic nitrogenous substances in biofloc technology(BFT)systems is critical for the success of this novel technology.To effectively control nitrogen accumulation in BFT systems,it is important to first unde...Controlling toxic nitrogenous substances in biofloc technology(BFT)systems is critical for the success of this novel technology.To effectively control nitrogen accumulation in BFT systems,it is important to first understand the dynamics and the removal pathways of this element and its related compounds from aquaculture water.This review focuses on synthesizing the information of nitrogen dynamics in BFT systems to provide researchers and practitioners with a guide to the fate of nitrogen and its control methods.This paper discusses the different types of nitrogenous compounds in BFT water,the transformation processes of ammonia to nitrites and nitrates,the relationship between the two forms of ammonia(NH3 and NH4+)in water and the equilibrium between them.This paper also discusses nitrification as a major nitrogen removal pathway and the factors that influence the nitrification process.Notably,the control of nitrogen in BFT systems by manipulating the carbon to nitrogen ratio(C/N)using external carbohydrates is described in this paper.This paper suggests that further studies should focus on investigating the various factors that influence nitrogen dynamics in BFT systems and the means of controlling contaminants other than nitrogen.展开更多
Recirculating Aquaculture System(RAS)is introduced in aquaculture farming industry to reduce water resource utilization,efficient the energy and land uses,and also help minimalize the water exchange.This system enable...Recirculating Aquaculture System(RAS)is introduced in aquaculture farming industry to reduce water resource utilization,efficient the energy and land uses,and also help minimalize the water exchange.This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry.Furthermore,this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment.As today,RAS has been developed with more effective technologies such as the use of UV irradiation,solid capture,protein skimmer and also provided with highly techno bio-filtration set.Basically,this system was applied for broodstock maturation,nursery phase,and grow-out production.In this review article,we provide an overview of RAS between the clear water,probiotic,and biofloc technology,and the advantages of its combination.Even though RAS and biofloc is two different parallel system,the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated.The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper.Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.展开更多
The biofloc technology(BFT)offers benefits in improving feed utilization and reducing the feed conversion ratio compared to the recirculating aquaculture system(RAS).In this study,high-throughput 16S rRNA gene sequenc...The biofloc technology(BFT)offers benefits in improving feed utilization and reducing the feed conversion ratio compared to the recirculating aquaculture system(RAS).In this study,high-throughput 16S rRNA gene sequencing was employed to investigate and compare the bacterial communities in these aquaculture systems and the gut microbiota of grass carp reared in them.We observed a significant distinction between the microbial communities of the aquaculture systems and the intestinal microbiota of grass carp.The genera cetobacterium and bacillus were more abundant in the BFT system,accounting for only 0.05% and 0.25% in the RAS.Our study also demonstrated that BFT could influence the intestinal microorganisms of grass carp(Ctenopharyngodon Idella)by reducing the relative abundance of burkholderia-caballeronia-paraburkholderia and increasing that of cetobacterium.Based on bugBase community predictions,the potential pathogenicity in BFT and grass carp intestine was lower than in RAS.Regarding gene expression in the grass carp intestine,immunosuppressive genes showed higher expression,while pro-inflammatory genes exhibited lower expression in grass carp reared in BFT.Furthermore,the final average length and weight of grass carp in the BFT system were significantly higher than those in the RAS.This study provides valuable insights into gut microbiome ecology in relation to two grass carp aquaculture systems,which could be effectively applied in grass carp management to promote health and improve water quality.展开更多
基金Supported by the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT_17R81)the Project of Agricultural intelligence introduction of Tibet(No.2020WZ006)。
文摘Biofl oc technology(BFT)improves water quality,and productivity of the farmed species through converting ammonium nitrogen to microbial protein,stabilizing microbial community,and reducing the production cost.In this study,a small-scale biofl oc development unit was designed in combination of recirculation system(RAS)for Artemia culture.Artemia growth,water quality,and microbial composition of biofl ocs in RAS were studied in comparison with in-situ batch culture(Glu).Glucose was added in RAS and Glu at C/N ratio of 10.The cultures without glucose addition,but with 50%daily water renewal(WRe)and without water renewal(NWRe)were considered as the controls.Arte mia were cultured at 25℃ and salinity 30 for 24 days and fed formulated feed.The results showed that compared to the controls,Glu signifi cantly improved the Artemia biomass,increased the biofl oc volume,and reduced the content of total ammonia nitrogen(TAN),nitrite nitrogen(NO_(2)-N)and nitrate nitrogen(NO_(3)-N)in water column(P<0.05).In addition,RAS had similar results with Glu.High throughput sequencing analysis on biofl oc microbial composition demonstrated that glucose supplement shaped the microbial community structure,and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth.Furthermore,we analyzed the relationship between the microbial composition of biofl oc and environmental factors.Canonical correspondence analysis(CCA)indicated that inorganic nitrogen in culture water had great impact on biofl oc microbial composition in NWRe and WRe,whilst the dissolved organic carbon(DOC)modifi ed the microbial community in Glu and RAS.This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.
文摘The growth of aquaculture demands intense consumption of formulated foods,scarce natural resources such as water and land.The increase in aquaculture production needs to be sustainable in several aspects of the activity,including the use of more sustainable farming systems that provide reduced water demand,less space dependence for increased production,and availability of complementary natural food.In this perspective,the biofloc system(BFT),presents itself as a more friendly cultivation technology as a way to mitigate some impacts of aquaculture production.Much research has been carried out,addressing different aspects important to the production of fish in BFT.In this review,we address how stocking density,carbon sources and carbon nitrogen ration(C/N)affect animal performance and the nutritional value of bioflocs;the use of microbial aggregates as“in situ”and“ex situ”food and the benefits in feed conversion,and their influence on the immune system and disease resistance.
基金This study was funded by the Shanghai Science and Technology Commission Project(19DZ2284300).
文摘Controlling toxic nitrogenous substances in biofloc technology(BFT)systems is critical for the success of this novel technology.To effectively control nitrogen accumulation in BFT systems,it is important to first understand the dynamics and the removal pathways of this element and its related compounds from aquaculture water.This review focuses on synthesizing the information of nitrogen dynamics in BFT systems to provide researchers and practitioners with a guide to the fate of nitrogen and its control methods.This paper discusses the different types of nitrogenous compounds in BFT water,the transformation processes of ammonia to nitrites and nitrates,the relationship between the two forms of ammonia(NH3 and NH4+)in water and the equilibrium between them.This paper also discusses nitrification as a major nitrogen removal pathway and the factors that influence the nitrification process.Notably,the control of nitrogen in BFT systems by manipulating the carbon to nitrogen ratio(C/N)using external carbohydrates is described in this paper.This paper suggests that further studies should focus on investigating the various factors that influence nitrogen dynamics in BFT systems and the means of controlling contaminants other than nitrogen.
基金the HICoE outcome project entitle"Indoor Multi-Techno Aquaculture System(IMTAS)",funded by the Ministry of Higher Education(MOHE),Malaysia under Higher Institution Centre of Excellence(HICoE),Institute of Tropical Aquaculture and Fisheries(AKUATROP),Universiti Malaysia Terengganu program[Vot.No.63933,JPT.S(BPKI)2000/016/018/015Jld.3(23)and Vot.No.56050,UMT/PPPI-2-2/5 Jld.2(24)].
文摘Recirculating Aquaculture System(RAS)is introduced in aquaculture farming industry to reduce water resource utilization,efficient the energy and land uses,and also help minimalize the water exchange.This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry.Furthermore,this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment.As today,RAS has been developed with more effective technologies such as the use of UV irradiation,solid capture,protein skimmer and also provided with highly techno bio-filtration set.Basically,this system was applied for broodstock maturation,nursery phase,and grow-out production.In this review article,we provide an overview of RAS between the clear water,probiotic,and biofloc technology,and the advantages of its combination.Even though RAS and biofloc is two different parallel system,the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated.The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper.Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.
基金supported by the“Key R&D Program of Guangdong Province,China,China Agriculture Research System of MOF and MARA,National Natural Science Foundation of China Youth Project”with grant number“2020B0202010001,CARS-45-03 and 31802285”.
文摘The biofloc technology(BFT)offers benefits in improving feed utilization and reducing the feed conversion ratio compared to the recirculating aquaculture system(RAS).In this study,high-throughput 16S rRNA gene sequencing was employed to investigate and compare the bacterial communities in these aquaculture systems and the gut microbiota of grass carp reared in them.We observed a significant distinction between the microbial communities of the aquaculture systems and the intestinal microbiota of grass carp.The genera cetobacterium and bacillus were more abundant in the BFT system,accounting for only 0.05% and 0.25% in the RAS.Our study also demonstrated that BFT could influence the intestinal microorganisms of grass carp(Ctenopharyngodon Idella)by reducing the relative abundance of burkholderia-caballeronia-paraburkholderia and increasing that of cetobacterium.Based on bugBase community predictions,the potential pathogenicity in BFT and grass carp intestine was lower than in RAS.Regarding gene expression in the grass carp intestine,immunosuppressive genes showed higher expression,while pro-inflammatory genes exhibited lower expression in grass carp reared in BFT.Furthermore,the final average length and weight of grass carp in the BFT system were significantly higher than those in the RAS.This study provides valuable insights into gut microbiome ecology in relation to two grass carp aquaculture systems,which could be effectively applied in grass carp management to promote health and improve water quality.
