Peripheral nerve injury(PNI)is a common disease that is difficult to nerve regeneration with current therapies.Fortunately,Zou et al demonstrated the role and mechanism of bone marrow derived mesenchymal stem cells(BM...Peripheral nerve injury(PNI)is a common disease that is difficult to nerve regeneration with current therapies.Fortunately,Zou et al demonstrated the role and mechanism of bone marrow derived mesenchymal stem cells(BMSCs)in promoting nerve regeneration,revealing broad prospects for BMSCs trans-plantation in alleviating PNI.We confirmed the fact that BMSCs significantly alleviate PNI,but there are shortcomings such as low cell survival rate and immune rejection,which limit the wide application of BMSCs.BMSCs-derived exosomes(Exos)are considered as a promising cell-free nanomedicine for PNI,avoiding the ethical issues of BMSCs.Exos in combination with bioengineering therapeutics(including extracellular matrix,hydrogel)brings new hope for PNI,provides a favorable microenvironment for neurological restoration and a therapeutic strategy with a favorable safety profile,significantly increases ex-pression of neurotrophic factors,promotes axonal and myelin regeneration,and demonstrates a strong potential to enhance neurogenesis.Therefore,engineered Exos exhibit better properties,such as stronger targeting and more beneficial components.This article briefly describes the role of nanotechnology and bioe-ngineering therapies for BMSCs in PNI,proposes clinical application prospects and challenges of nanotechnology and bioengineering BMSCs-derived Exos in PNI to improve the efficacy of BMSCs in the treatment of PNI.展开更多
The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed ...The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed that while employment placement rates for bioengineering graduates remain stable,starting salaries exhibit limited growth and career trajectories lack clarity.These challenges originate from a curriculum lagging behind technological progress,inadequate practical training,and a mismatch between student competencies and industry expectations.To address these issues,this paper proposed a strategic framework grounded in"demand-driven design,industry-education integration,and competence-centered development".Key strategies include dynamic curriculum renewal,collaborative university-industry training,holistic competency development,and personalized student support,which collectively aim at enhancing graduates employment competitiveness and long-term professional sustainability.展开更多
Gulf War Illness(GWI)is characterized by a wide range of symptoms that manifests largely as gastrointestinal symptoms.Among these gastrointestinal symptoms,motility disorders are highly prevalent,presenting as chronic...Gulf War Illness(GWI)is characterized by a wide range of symptoms that manifests largely as gastrointestinal symptoms.Among these gastrointestinal symptoms,motility disorders are highly prevalent,presenting as chronic constipation,stomach pain,indigestion,diarrhea,and other conditions that severely impact the quality of life of GWI veterans.However,despite a high prevalence of gastrointestinal impairments among these veterans,most research attention has focused on neurological disturbances.This perspective provides a comprehensive overview of current in vivo research advancements elucidating the underlying mechanisms contributing to gastrointestinal disorders in GWI.Generally,these in vivo and in vitro models propose that neuroinflammation alters gut motility and drives the gastrointestinal symptoms reported in GWI.Additionally,this perspective highlights the potential and challenges of in vitro bioengineering models,which could be a crucial contributor to understanding and treating the pathology of gastrointestinal related-GWI.展开更多
Recent advancements in DNA and RNA bioengineering have paved the way for developing stimuli-responsive nanostructures with remarkable potential across various applications.These nanostructures,crafted through sophisti...Recent advancements in DNA and RNA bioengineering have paved the way for developing stimuli-responsive nanostructures with remarkable potential across various applications.These nanostructures,crafted through sophisticated bioengineering techniques,can dynamically and precisely respond to both physiological and physical stimuli,including nucleic acids(DNA/RNA),adenosine triphosphate,proteins,ions,small molecules,pH,light,and temperature.They offer high sensitivity and specificity,making them ideal for applications such as biomarker detection,gene therapy,and controlled targeted drug delivery.In this review,we summarize the bioengineering methods used to assemble versatile stimuli-responsive DNA/RNA nanostructures and discuss their emerging applications in structural biology and biomedicine,including biosensing,targeted drug delivery,and therapeutics.Finally,we highlight the challenges and opportunities in the rational design of these intelligent bioengineered nanostructures.展开更多
In the context of emerging engineering disciplines,a hybrid teaching reform for the Bioengineering Downstream Technology course,based on ideological and political education and online open courses,is being carried out...In the context of emerging engineering disciplines,a hybrid teaching reform for the Bioengineering Downstream Technology course,based on ideological and political education and online open courses,is being carried out.This reform focuses on aspects such as“building a professional teacher team for ideological and political education,scientifically designing the ideological and political teaching system,innovating classroom teaching methods,and improving both formative and summative evaluation systems.”The“Craftsmanship in Education and Cultivating Soul and Roots”small private online course hybrid teaching reform for the Bioengineering Downstream Technology online open course provides a replicable model for the comprehensive implementation of ideological and political education in engineering courses and offers a reference for advancing ideological and political education and hybrid teaching reform in new engineering disciplines.展开更多
Peripheral nerve injuries remain a challenging problem in need of better treatment strategies.Despite best efforts at surgical reconstruction and postoperative rehabilitation,patients are often left with persistent,de...Peripheral nerve injuries remain a challenging problem in need of better treatment strategies.Despite best efforts at surgical reconstruction and postoperative rehabilitation,patients are often left with persistent,debilitating motor and sensory deficits.There are currently no therapeutic strategies proven to enhance the regenerative process in humans.A clinical need exists for the development of technologies to promote nerve regeneration and improve functional outcomes.Recent advances in the fields of tissue engineering and nanotechnology have enabled biomaterial scaffolds to modulate the host response to tissue repair through tailored mechanical,chemical,and conductive cues.New bioengineered approaches have enabled targeted,sustained delivery of protein therapeutics with the capacity to unlock the clinical potential of a myriad of neurotrophic growth factors that have demonstrated promise in enhancing regenerative outcomes.As such,further exploration of combinatory strategies leveraging these technological advances may offer a pathway towards clinically translatable solutions to advance the care of patients with peripheral nerve injuries.This review first presents the various emerging bioengineering strategies that can be applied for the management of nerve gap injuries.We cover the rationale and limitations for their use as an alternative to autografts,focusing on the approaches to increase the number of regenerating axons crossing the repair site,and facilitating their growth towards the distal stump.We also discuss the emerging growth factor-based therapeutic strategies designed to improve functional outcomes in a multimodal fashion,by accelerating axonal growth,improving the distal regenerative environment,and preventing end-organs atrophy.展开更多
The female reproductive system consists of the ovaries,the female gonads,and the reproductive tract organs of the fallopian tubes,uterus,cervix,and vagina.It functions to provide hormonal support and anatomical struct...The female reproductive system consists of the ovaries,the female gonads,and the reproductive tract organs of the fallopian tubes,uterus,cervix,and vagina.It functions to provide hormonal support and anatomical structure for the production of new offspring.A number of endogenous and exogenous factors can impact female reproductive health and fertility,including genetic vulnerability,medications,environmental exposures,age,nutrition,and diseases.To date,due to the ethical concerns of using human subjects in biomedical research,the majority of studies use in vivo animal models and 2D cell/tissue culture models to study female reproduction.However,the complexity and species difference of the female reproductive system in humans make it difficult to compare to those of animals.Moreover,the monolayered cells cultured on flat plastics or glass lose their 3D architecture as well as the physical and/or biochemical contacts with other cells in vivo.Further,all reproductive organs do not work alone but interconnect with each other and also with non-reproductive organs to support female reproductive,endocrine,and systemic health.These facts suggest that there is an urgent and unmet need to develop representative,effective,and efficient in vitro models for studying human female reproduction.The prodigious advancements of bioengineering(e.g.,biomaterials,3D printing,and organ-on-a-chip)allow us to study female reproduction in an entirely new way.Here,we review recent advances that use bioengineering methods to study female reproduction,including the bioengineering models of the ovary,fallopian tube,uterus,embryo implantation,placenta,and reproductive disease.展开更多
Liver transplantation is the only curative therapy for end stage liver disease,but is limited by the organ shortage,and is associated with the adverse consequences of immunosuppression.Repopulation of decellularised w...Liver transplantation is the only curative therapy for end stage liver disease,but is limited by the organ shortage,and is associated with the adverse consequences of immunosuppression.Repopulation of decellularised whole organ scaffolds with appropriate cells of recipient origin offers a theoretically attractive solution,allowing reliable and timely organ sourcing without the need for immunosuppression.Decellularisation methodologies vary widely but seek to address the conflicting objectives of removing the cellular component of tissues whilst keeping the 3D structure of the extra-cellular matrix intact,as well as retaining the instructive cell fate determining biochemicals contained therein.Liver scaffold recellularisation has progressed from small rodent in vitro studies to large animal in vivo perfusion models,using a wide range of cell types including primary cells,cell lines,foetal stem cells,and induced pluripotent stem cells.Within these models,a limited but measurable degree of physiologically significant hepatocyte function has been reported with demonstrable ammonia metabolism in vivo.Biliary repopulation and function have been restricted by challenges relating to the culture and propagations of cholangiocytes,though advances in organoid culture may help address this.Hepatic vasculature repopulation has enabled sustainable blood perfusion in vivo,but with cell types that would limit clinical applications,and which have not been shown to have the specific functions of liver sinusoidal endothelial cells.Minority cell groups such as Kupffer cells and stellate cells have not been repopulated.Bioengineering by repopulation of decellularised scaffolds has significantly progressed,but there remain significant experimental challenges to be addressed before therapeutic applications may be envisaged.展开更多
This paper deals with the study of fractional order system tuning method based on Factional Order Proportional Integral Derivative( FOPID) controller in allusion to the nonlinear characteristics and fractional order m...This paper deals with the study of fractional order system tuning method based on Factional Order Proportional Integral Derivative( FOPID) controller in allusion to the nonlinear characteristics and fractional order mathematical model of bioengineering systems. The main contents include the design of FOPID controller and the simulation for bioengineering systems. The simulation results show that the tuning method of fractional order system based on the FOPID controller outperforms the fractional order system based on Fractional Order Proportional Integral( FOPI) controller. As it can enhance control character and improve the robustness of the system.展开更多
The dependence to fossil fuels has increased the amount of greenhouse gases in the atmosphere.That is why,the production of renewable and sustainable biofuels has gained a long-term importance for both scientific and ...The dependence to fossil fuels has increased the amount of greenhouse gases in the atmosphere.That is why,the production of renewable and sustainable biofuels has gained a long-term importance for both scientific and political necessities.In this context,algae are promising in terms of alternative biofuels resources.For this reason,intensive scientific researches have been carried out in recent years on providing optimum efficiency in this regard.Bioengineering is a discipline that applies engineering principles of design and analysis to biological systems and biomedical technologies.Examples of bioengineering research include bacteria or microalgae engineered to produce valuable bioactive chemicals.Microalgae by target gene modification may serve as a promising source for the production of biofuels and bio-based chemicals.A lot of research has been carried out by applying microalgae genomic editing technique with the aim to produce numerous biotechnological products.Some successful previously reported research and production activities are still underway in this area.However,in order to produce the desired products efficiently with manipulated microalgae biorefinery,there is a need to overcome the problem of low biomass production despite high production costs.The aim of this work is to give special attention to the rich potential content of microalgae and to provide information on algal genetic manipulations to increase products by bioengineering methods.展开更多
Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultura...Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultural reformation inside and outside the college and reform of off-campus teaching experiment farm in view of outstanding agricultural characteristics of the bioengineering branch, with environmental health preservation as the fundamental goal and "health-preserving agriculture" as the link. In addition, four major characteristic landscape frameworks of "point, line, surface, and body" were established, and the effective construction approaches of health-preserving environmental cultural landscapes in vocational colleges were explored.展开更多
Corneal blindness represents one of the world’s three major causes of blindness,and the fundamental problem of corneal transplantation is a severe shortage of donor tissues worldwide,resulting in approximately 1.5 mi...Corneal blindness represents one of the world’s three major causes of blindness,and the fundamental problem of corneal transplantation is a severe shortage of donor tissues worldwide,resulting in approximately 1.5 million new cases of blindness annually.To address the growing need for corneal transplants two main approaches are being pursued:allogenic and bioengineering cornea.Bioengineering corneas are constructed by naturally generating an extracellular matrix(ECM)component as the scaffold structure with or without corneal cells.It is well established that the scaffold structure directs the fate of cells,therefore,the fabrication of the correct scaffold structure components could produce an ideal corneal substitute,able to mimic the native corneal function.Another key factor in the construction of tissue engineering cornea is seed cells.However,unlike the epithelium and stroma cells,human cornea endothelium cells(HCECs)are notorious for having a limited proliferative capacity in vivo because of the mitotic block at the G1 phase of the cell cycle due to“contact-inhibition”.This review will focus on the main concepts of recent progress towards the scaffold and seed cells,especially endothelial cells for bioengineering cornea,along with future perspectives.展开更多
Chronic kidney disease is currently a major public health problem around the world. Although hemodialysis increases survival of patients with end-stage renal disease, kidney transplantation remains the only potentiall...Chronic kidney disease is currently a major public health problem around the world. Although hemodialysis increases survival of patients with end-stage renal disease, kidney transplantation remains the only potentially curative treatment. However, transplantation as a therapeutic option is limited by availability of suitable donor organs. This situation highlights the urgent need to find new and potentially inexhaustible sources of transplantable organs. Perfusion decellularizarion of whole organs is a novel approach to organ engineering and regeneration. In the present research, we used a continuous perfusion decellularization protocol to eliminate cellular componet of kidney and evaluated residual scaffold components after decellularizarion process by proteomics analysis. Our proteomic data show that this protocol results in incomplete removal of cellular proteins. However, unlike other authors, we assume that proteins retained within decellularized kidney scaffold could be the basis for specific homing and celular differentation in the recellularization process.展开更多
Inadequate absorptive surface area poses a great challenge to the patients suffering a variety of in-testinal diseases causing short bowel syndrome. To date, these patients are managed with total parenteral nutrition ...Inadequate absorptive surface area poses a great challenge to the patients suffering a variety of in-testinal diseases causing short bowel syndrome. To date, these patients are managed with total parenteral nutrition or intestinal transplantation. However, these carry significant morbidity and mortality. Currently, by emergence of tissue engineering, anticipations to utilize an alternative method to increase the intestinal absorptive surface area are increasing. In this paper, we will review the improvements made over time in attempting elongating the intestine with surgical techniques as well as using intestinal bioengineering. Performing sequential intestinal lengthening was the preliminary method applied in humans. However, these methods did not reach widespread use and has limited outcome. Subsequent experimental methods were developed utilizing scaffolds to regenerate intestinal tissue and organoids unit from the intestinal epithelium. Stem cells also have been studied and applied in all types of tissue engineering. Biomaterials were utilized as a structural support for naive cells to produce bio-engineered tissue that can achieve a near-normal anatomical structure. A promising novel approach is the elongation of the intestine with an acellular biologic scaffold to generate a neoformed intestinal tissue that showed, for the first time, evidence of absorption in vivo. In the large intestine, studies are more focused on regeneration and engineering of sphincters and will be briefly reviewed. From the review of the existing literature, it can be concluded that significant progress has been achieved in these experimental methods but that these now need to be fully translated into a pre-clinical and clinical experimentation to become a future viable therapeutic option.展开更多
Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increas...Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increased fundamental knowledge on starch biosynthesis,development of advanced breeding strategies,efficient farming,and well-adapted and up scalable extraction protocols for diverse starch products.Recent staggering progress in molecular breeding techniques,especially genome editing,have enabled generation of higher starch yield and special functional qualities required to support such advancement.However,this necessitates fundamental biochemical and mechanistic understanding of starch biosynthesis and the variegated starch crop germplasms,all of which are closely linked to the relationships between starch molecular structures and functionality of various starch types as directed by the different capabilities of starch crop genotypes.We here review starch biosynthesis and its genetic foundation with a focus on increasing nutritional and health-promoting value of starch especially through bioengineering of the high amylose trait.展开更多
The advancement of bimetallic catalysts holds significant promise for the innovation of oxygen evolution reaction(OER)catalysts.Drawing from adsorbate evolution mechanism(AEM)and lattice oxygen oxidation mechanism(LOM...The advancement of bimetallic catalysts holds significant promise for the innovation of oxygen evolution reaction(OER)catalysts.Drawing from adsorbate evolution mechanism(AEM)and lattice oxygen oxidation mechanism(LOM),the incorporation of dual active sites has the potential to foster novel OER pathways,such as the coupled oxygen evolution mechanism(COM),which can surpass the limitations of OER and elevate catalytic performance.In this study,uniformly distributed Fe/Ni dual-site Fe-Ni_(2)P@C electrocatalysts are crafted by upcycling metals in electroplating sludge via an eco-friendly and sustainable microbial engineering technique.Our findings indicate that a substantial number of defects emerge at the Ni2P crystal during the OER process,laying the groundwork for lattice oxygen involvement.Moreover,the displacement of Ni/Fe in the crystal lattice intensifies the asymmetry of the electronic structure at the metal active sites,facilitating the deprotonation process.This research introduces an innovative paradigm for the synthesis of effective and robust transition metal-based OER catalysts,with implications for sustainable energy generation technologies.展开更多
Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied fo...Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord injury.Each of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional recovery.Although the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in humans.This article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were analyzed.Thus,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.展开更多
Under the background of the continuous deepening of engineering education accreditation and the construction of emerging engineering disciplines,a digital platform-assisted teaching model was explored for the teaching...Under the background of the continuous deepening of engineering education accreditation and the construction of emerging engineering disciplines,a digital platform-assisted teaching model was explored for the teaching reform of the molecular biology course to effectively support the achievement of graduation requirements for bioengineering major and enhance the teaching outcomes of the molecular biology course.The teaching reform of this course took the 2022 cohort students majoring in bioengineering in Chengdu University as the practice object.The course evaluation method was improved by integrating digital platforms for process assessment,and real-world research and practical cases were incorporated into digital platforms to enrich teaching resources.Additionally,digital platforms were integrated throughout the entire teaching process(before,during,and after class),reshaping the instructional workflow into"pre-class online self-learning,in-class teacher-student interaction for deepening knowledge internalization,and practical case studies during and after class for strengthening application".The teaching reform results demonstrated that this teaching model significantly improved the attainment of course objectives,providing valuable experience for similar institutions to advance digital course reforms under the framework of engineering education accreditation.展开更多
Welcome to the 4th volume of Biomedical Engineering Communications the first issue of 2025!Biomedical engineering is a rapidly evolving field that combines engineering principles with medical and biological sciences t...Welcome to the 4th volume of Biomedical Engineering Communications the first issue of 2025!Biomedical engineering is a rapidly evolving field that combines engineering principles with medical and biological sciences to create innovative healthcare technologies.Biomedical engineering brings an interdisciplinary,problem-solving approach to bioengineering,biology and medicine.This interdisciplinary field is essential for developing advanced medical devices,diagnostic tools,and therapeutic solutions that enhance patient care and improve health outcomes.It allows them to develop technologies and systems that directly contribute to diagnosing,treating and preventing diseases.展开更多
The brain is the most complex human organ,and commonly used models,such as two-dimensional-cell cultures and animal brains,often lack the sophistication needed to accurately use in research.In this context,human cereb...The brain is the most complex human organ,and commonly used models,such as two-dimensional-cell cultures and animal brains,often lack the sophistication needed to accurately use in research.In this context,human cerebral organoids have emerged as valuable tools offering a more complex,versatile,and human-relevant system than traditional animal models,which are often unable to replicate the intricate architecture and functionality of the human brain.Since human cerebral organoids are a state-of-the-art model for the study of neurodevelopment and different pathologies affecting the brain,this field is currently under constant development,and work in this area is abundant.In this review,we give a complete overview of human cerebral organoids technology,starting from the different types of protocols that exist to generate different human cerebral organoids.We continue with the use of brain organoids for the study of brain pathologies,highlighting neurodevelopmental,psychiatric,neurodegenerative,brain tumor,and infectious diseases.Because of the potential value of human cerebral organoids,we describe their use in transplantation,drug screening,and toxicology assays.We also discuss the technologies available to study cell diversity and physiological characteristics of organoids.Finally,we summarize the limitations that currently exist in the field,such as the development of vasculature and microglia,and highlight some of the novel approaches being pursued through bioengineering.展开更多
基金Supported by the Tianjin Graduate Research Innovation Project&TUTCM Graduate Research Innovation Project,No.YJSKC-20231012.
文摘Peripheral nerve injury(PNI)is a common disease that is difficult to nerve regeneration with current therapies.Fortunately,Zou et al demonstrated the role and mechanism of bone marrow derived mesenchymal stem cells(BMSCs)in promoting nerve regeneration,revealing broad prospects for BMSCs trans-plantation in alleviating PNI.We confirmed the fact that BMSCs significantly alleviate PNI,but there are shortcomings such as low cell survival rate and immune rejection,which limit the wide application of BMSCs.BMSCs-derived exosomes(Exos)are considered as a promising cell-free nanomedicine for PNI,avoiding the ethical issues of BMSCs.Exos in combination with bioengineering therapeutics(including extracellular matrix,hydrogel)brings new hope for PNI,provides a favorable microenvironment for neurological restoration and a therapeutic strategy with a favorable safety profile,significantly increases ex-pression of neurotrophic factors,promotes axonal and myelin regeneration,and demonstrates a strong potential to enhance neurogenesis.Therefore,engineered Exos exhibit better properties,such as stronger targeting and more beneficial components.This article briefly describes the role of nanotechnology and bioe-ngineering therapies for BMSCs in PNI,proposes clinical application prospects and challenges of nanotechnology and bioengineering BMSCs-derived Exos in PNI to improve the efficacy of BMSCs in the treatment of PNI.
基金Supported by Undergraduate Education and Teaching Reform Research Project of Chengdu University(XJJG-20242025228)Sichuan Genuine Medicinal Materials and Traditional Chinese Medicine Innovation Team(SCCXTD-2025-19)Sichuan Science and Technology Program(2021YFYZ0012).
文摘The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed that while employment placement rates for bioengineering graduates remain stable,starting salaries exhibit limited growth and career trajectories lack clarity.These challenges originate from a curriculum lagging behind technological progress,inadequate practical training,and a mismatch between student competencies and industry expectations.To address these issues,this paper proposed a strategic framework grounded in"demand-driven design,industry-education integration,and competence-centered development".Key strategies include dynamic curriculum renewal,collaborative university-industry training,holistic competency development,and personalized student support,which collectively aim at enhancing graduates employment competitiveness and long-term professional sustainability.
基金supported by the Congressionally Directed Medical Research Program Award through the Gulf War Illness Research Program (SAR, W81XWH-21±1-0477)funding from the Aviles-Johnson Doctoral Diversity Fellowship Awardthe National Defense Science and Engineering Graduate Fellowship Awards (CAC)
文摘Gulf War Illness(GWI)is characterized by a wide range of symptoms that manifests largely as gastrointestinal symptoms.Among these gastrointestinal symptoms,motility disorders are highly prevalent,presenting as chronic constipation,stomach pain,indigestion,diarrhea,and other conditions that severely impact the quality of life of GWI veterans.However,despite a high prevalence of gastrointestinal impairments among these veterans,most research attention has focused on neurological disturbances.This perspective provides a comprehensive overview of current in vivo research advancements elucidating the underlying mechanisms contributing to gastrointestinal disorders in GWI.Generally,these in vivo and in vitro models propose that neuroinflammation alters gut motility and drives the gastrointestinal symptoms reported in GWI.Additionally,this perspective highlights the potential and challenges of in vitro bioengineering models,which could be a crucial contributor to understanding and treating the pathology of gastrointestinal related-GWI.
文摘Recent advancements in DNA and RNA bioengineering have paved the way for developing stimuli-responsive nanostructures with remarkable potential across various applications.These nanostructures,crafted through sophisticated bioengineering techniques,can dynamically and precisely respond to both physiological and physical stimuli,including nucleic acids(DNA/RNA),adenosine triphosphate,proteins,ions,small molecules,pH,light,and temperature.They offer high sensitivity and specificity,making them ideal for applications such as biomarker detection,gene therapy,and controlled targeted drug delivery.In this review,we summarize the bioengineering methods used to assemble versatile stimuli-responsive DNA/RNA nanostructures and discuss their emerging applications in structural biology and biomedicine,including biosensing,targeted drug delivery,and therapeutics.Finally,we highlight the challenges and opportunities in the rational design of these intelligent bioengineered nanostructures.
基金Guangdong Province Undergraduate Online Open Course Guidance Committee Research Project(2022ZXKC462)Foshan Philosophy and Social Science Planning Project(2024-GJ 037)+4 种基金Provincial First-Class Undergraduate Courses of Guangdong Provincial Education Department(Guangdong Education Gaohan[2022]No.10)Innovation Project of Guangdong Graduate Education(2022JGXM129,2022JGXM128,2023ANLK-080)Foshan University Curriculum Ideological and Political Teaching Reform and Practice Demonstration Project in 2023Quality Engineering Project of Foshan University in 2023Collaborative Education Project of the Ministry of Education in 2023(230703232312141)。
文摘In the context of emerging engineering disciplines,a hybrid teaching reform for the Bioengineering Downstream Technology course,based on ideological and political education and online open courses,is being carried out.This reform focuses on aspects such as“building a professional teacher team for ideological and political education,scientifically designing the ideological and political teaching system,innovating classroom teaching methods,and improving both formative and summative evaluation systems.”The“Craftsmanship in Education and Cultivating Soul and Roots”small private online course hybrid teaching reform for the Bioengineering Downstream Technology online open course provides a replicable model for the comprehensive implementation of ideological and political education in engineering courses and offers a reference for advancing ideological and political education and hybrid teaching reform in new engineering disciplines.
基金supported by The Plastic Surgery Foundation Research Pilot Grant,No.627383(to KAS).
文摘Peripheral nerve injuries remain a challenging problem in need of better treatment strategies.Despite best efforts at surgical reconstruction and postoperative rehabilitation,patients are often left with persistent,debilitating motor and sensory deficits.There are currently no therapeutic strategies proven to enhance the regenerative process in humans.A clinical need exists for the development of technologies to promote nerve regeneration and improve functional outcomes.Recent advances in the fields of tissue engineering and nanotechnology have enabled biomaterial scaffolds to modulate the host response to tissue repair through tailored mechanical,chemical,and conductive cues.New bioengineered approaches have enabled targeted,sustained delivery of protein therapeutics with the capacity to unlock the clinical potential of a myriad of neurotrophic growth factors that have demonstrated promise in enhancing regenerative outcomes.As such,further exploration of combinatory strategies leveraging these technological advances may offer a pathway towards clinically translatable solutions to advance the care of patients with peripheral nerve injuries.This review first presents the various emerging bioengineering strategies that can be applied for the management of nerve gap injuries.We cover the rationale and limitations for their use as an alternative to autografts,focusing on the approaches to increase the number of regenerating axons crossing the repair site,and facilitating their growth towards the distal stump.We also discuss the emerging growth factor-based therapeutic strategies designed to improve functional outcomes in a multimodal fashion,by accelerating axonal growth,improving the distal regenerative environment,and preventing end-organs atrophy.
基金This work is supported by the National Institutes of Health(NIH K01ES030014 and P01ES028942)National Science Foundation(NSF 183291)。
文摘The female reproductive system consists of the ovaries,the female gonads,and the reproductive tract organs of the fallopian tubes,uterus,cervix,and vagina.It functions to provide hormonal support and anatomical structure for the production of new offspring.A number of endogenous and exogenous factors can impact female reproductive health and fertility,including genetic vulnerability,medications,environmental exposures,age,nutrition,and diseases.To date,due to the ethical concerns of using human subjects in biomedical research,the majority of studies use in vivo animal models and 2D cell/tissue culture models to study female reproduction.However,the complexity and species difference of the female reproductive system in humans make it difficult to compare to those of animals.Moreover,the monolayered cells cultured on flat plastics or glass lose their 3D architecture as well as the physical and/or biochemical contacts with other cells in vivo.Further,all reproductive organs do not work alone but interconnect with each other and also with non-reproductive organs to support female reproductive,endocrine,and systemic health.These facts suggest that there is an urgent and unmet need to develop representative,effective,and efficient in vitro models for studying human female reproduction.The prodigious advancements of bioengineering(e.g.,biomaterials,3D printing,and organ-on-a-chip)allow us to study female reproduction in an entirely new way.Here,we review recent advances that use bioengineering methods to study female reproduction,including the bioengineering models of the ovary,fallopian tube,uterus,embryo implantation,placenta,and reproductive disease.
文摘Liver transplantation is the only curative therapy for end stage liver disease,but is limited by the organ shortage,and is associated with the adverse consequences of immunosuppression.Repopulation of decellularised whole organ scaffolds with appropriate cells of recipient origin offers a theoretically attractive solution,allowing reliable and timely organ sourcing without the need for immunosuppression.Decellularisation methodologies vary widely but seek to address the conflicting objectives of removing the cellular component of tissues whilst keeping the 3D structure of the extra-cellular matrix intact,as well as retaining the instructive cell fate determining biochemicals contained therein.Liver scaffold recellularisation has progressed from small rodent in vitro studies to large animal in vivo perfusion models,using a wide range of cell types including primary cells,cell lines,foetal stem cells,and induced pluripotent stem cells.Within these models,a limited but measurable degree of physiologically significant hepatocyte function has been reported with demonstrable ammonia metabolism in vivo.Biliary repopulation and function have been restricted by challenges relating to the culture and propagations of cholangiocytes,though advances in organoid culture may help address this.Hepatic vasculature repopulation has enabled sustainable blood perfusion in vivo,but with cell types that would limit clinical applications,and which have not been shown to have the specific functions of liver sinusoidal endothelial cells.Minority cell groups such as Kupffer cells and stellate cells have not been repopulated.Bioengineering by repopulation of decellularised scaffolds has significantly progressed,but there remain significant experimental challenges to be addressed before therapeutic applications may be envisaged.
文摘This paper deals with the study of fractional order system tuning method based on Factional Order Proportional Integral Derivative( FOPID) controller in allusion to the nonlinear characteristics and fractional order mathematical model of bioengineering systems. The main contents include the design of FOPID controller and the simulation for bioengineering systems. The simulation results show that the tuning method of fractional order system based on the FOPID controller outperforms the fractional order system based on Fractional Order Proportional Integral( FOPI) controller. As it can enhance control character and improve the robustness of the system.
文摘The dependence to fossil fuels has increased the amount of greenhouse gases in the atmosphere.That is why,the production of renewable and sustainable biofuels has gained a long-term importance for both scientific and political necessities.In this context,algae are promising in terms of alternative biofuels resources.For this reason,intensive scientific researches have been carried out in recent years on providing optimum efficiency in this regard.Bioengineering is a discipline that applies engineering principles of design and analysis to biological systems and biomedical technologies.Examples of bioengineering research include bacteria or microalgae engineered to produce valuable bioactive chemicals.Microalgae by target gene modification may serve as a promising source for the production of biofuels and bio-based chemicals.A lot of research has been carried out by applying microalgae genomic editing technique with the aim to produce numerous biotechnological products.Some successful previously reported research and production activities are still underway in this area.However,in order to produce the desired products efficiently with manipulated microalgae biorefinery,there is a need to overcome the problem of low biomass production despite high production costs.The aim of this work is to give special attention to the rich potential content of microalgae and to provide information on algal genetic manipulations to increase products by bioengineering methods.
基金Sponsored by Humanities and Social Sciences Research Foundation of Yangling Vocational&Technical College(GJ1423)
文摘Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultural reformation inside and outside the college and reform of off-campus teaching experiment farm in view of outstanding agricultural characteristics of the bioengineering branch, with environmental health preservation as the fundamental goal and "health-preserving agriculture" as the link. In addition, four major characteristic landscape frameworks of "point, line, surface, and body" were established, and the effective construction approaches of health-preserving environmental cultural landscapes in vocational colleges were explored.
基金This work was supported by National Natural Science Foundation of China(Grants:81600708).
文摘Corneal blindness represents one of the world’s three major causes of blindness,and the fundamental problem of corneal transplantation is a severe shortage of donor tissues worldwide,resulting in approximately 1.5 million new cases of blindness annually.To address the growing need for corneal transplants two main approaches are being pursued:allogenic and bioengineering cornea.Bioengineering corneas are constructed by naturally generating an extracellular matrix(ECM)component as the scaffold structure with or without corneal cells.It is well established that the scaffold structure directs the fate of cells,therefore,the fabrication of the correct scaffold structure components could produce an ideal corneal substitute,able to mimic the native corneal function.Another key factor in the construction of tissue engineering cornea is seed cells.However,unlike the epithelium and stroma cells,human cornea endothelium cells(HCECs)are notorious for having a limited proliferative capacity in vivo because of the mitotic block at the G1 phase of the cell cycle due to“contact-inhibition”.This review will focus on the main concepts of recent progress towards the scaffold and seed cells,especially endothelial cells for bioengineering cornea,along with future perspectives.
文摘Chronic kidney disease is currently a major public health problem around the world. Although hemodialysis increases survival of patients with end-stage renal disease, kidney transplantation remains the only potentially curative treatment. However, transplantation as a therapeutic option is limited by availability of suitable donor organs. This situation highlights the urgent need to find new and potentially inexhaustible sources of transplantable organs. Perfusion decellularizarion of whole organs is a novel approach to organ engineering and regeneration. In the present research, we used a continuous perfusion decellularization protocol to eliminate cellular componet of kidney and evaluated residual scaffold components after decellularizarion process by proteomics analysis. Our proteomic data show that this protocol results in incomplete removal of cellular proteins. However, unlike other authors, we assume that proteins retained within decellularized kidney scaffold could be the basis for specific homing and celular differentation in the recellularization process.
文摘Inadequate absorptive surface area poses a great challenge to the patients suffering a variety of in-testinal diseases causing short bowel syndrome. To date, these patients are managed with total parenteral nutrition or intestinal transplantation. However, these carry significant morbidity and mortality. Currently, by emergence of tissue engineering, anticipations to utilize an alternative method to increase the intestinal absorptive surface area are increasing. In this paper, we will review the improvements made over time in attempting elongating the intestine with surgical techniques as well as using intestinal bioengineering. Performing sequential intestinal lengthening was the preliminary method applied in humans. However, these methods did not reach widespread use and has limited outcome. Subsequent experimental methods were developed utilizing scaffolds to regenerate intestinal tissue and organoids unit from the intestinal epithelium. Stem cells also have been studied and applied in all types of tissue engineering. Biomaterials were utilized as a structural support for naive cells to produce bio-engineered tissue that can achieve a near-normal anatomical structure. A promising novel approach is the elongation of the intestine with an acellular biologic scaffold to generate a neoformed intestinal tissue that showed, for the first time, evidence of absorption in vivo. In the large intestine, studies are more focused on regeneration and engineering of sphincters and will be briefly reviewed. From the review of the existing literature, it can be concluded that significant progress has been achieved in these experimental methods but that these now need to be fully translated into a pre-clinical and clinical experimentation to become a future viable therapeutic option.
文摘Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increased fundamental knowledge on starch biosynthesis,development of advanced breeding strategies,efficient farming,and well-adapted and up scalable extraction protocols for diverse starch products.Recent staggering progress in molecular breeding techniques,especially genome editing,have enabled generation of higher starch yield and special functional qualities required to support such advancement.However,this necessitates fundamental biochemical and mechanistic understanding of starch biosynthesis and the variegated starch crop germplasms,all of which are closely linked to the relationships between starch molecular structures and functionality of various starch types as directed by the different capabilities of starch crop genotypes.We here review starch biosynthesis and its genetic foundation with a focus on increasing nutritional and health-promoting value of starch especially through bioengineering of the high amylose trait.
基金supported by the National Natural Science Foundation of China(Nos.21905317 and U23B20166)the Young Elite Scientists Sponsorship Program by CAST(No.2019QNRC001)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University(No.76180-31620007).
文摘The advancement of bimetallic catalysts holds significant promise for the innovation of oxygen evolution reaction(OER)catalysts.Drawing from adsorbate evolution mechanism(AEM)and lattice oxygen oxidation mechanism(LOM),the incorporation of dual active sites has the potential to foster novel OER pathways,such as the coupled oxygen evolution mechanism(COM),which can surpass the limitations of OER and elevate catalytic performance.In this study,uniformly distributed Fe/Ni dual-site Fe-Ni_(2)P@C electrocatalysts are crafted by upcycling metals in electroplating sludge via an eco-friendly and sustainable microbial engineering technique.Our findings indicate that a substantial number of defects emerge at the Ni2P crystal during the OER process,laying the groundwork for lattice oxygen involvement.Moreover,the displacement of Ni/Fe in the crystal lattice intensifies the asymmetry of the electronic structure at the metal active sites,facilitating the deprotonation process.This research introduces an innovative paradigm for the synthesis of effective and robust transition metal-based OER catalysts,with implications for sustainable energy generation technologies.
文摘Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord injury.Each of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional recovery.Although the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in humans.This article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were analyzed.Thus,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.
基金Supported by 2023 Major Project for Talent Cultivation and Teaching Reform in Higher Education of Sichuan Province(JG2023-77)2024-2026 Undergraduate Education and Teaching Reform Project of Chengdu University(XJJG-20242025264).
文摘Under the background of the continuous deepening of engineering education accreditation and the construction of emerging engineering disciplines,a digital platform-assisted teaching model was explored for the teaching reform of the molecular biology course to effectively support the achievement of graduation requirements for bioengineering major and enhance the teaching outcomes of the molecular biology course.The teaching reform of this course took the 2022 cohort students majoring in bioengineering in Chengdu University as the practice object.The course evaluation method was improved by integrating digital platforms for process assessment,and real-world research and practical cases were incorporated into digital platforms to enrich teaching resources.Additionally,digital platforms were integrated throughout the entire teaching process(before,during,and after class),reshaping the instructional workflow into"pre-class online self-learning,in-class teacher-student interaction for deepening knowledge internalization,and practical case studies during and after class for strengthening application".The teaching reform results demonstrated that this teaching model significantly improved the attainment of course objectives,providing valuable experience for similar institutions to advance digital course reforms under the framework of engineering education accreditation.
文摘Welcome to the 4th volume of Biomedical Engineering Communications the first issue of 2025!Biomedical engineering is a rapidly evolving field that combines engineering principles with medical and biological sciences to create innovative healthcare technologies.Biomedical engineering brings an interdisciplinary,problem-solving approach to bioengineering,biology and medicine.This interdisciplinary field is essential for developing advanced medical devices,diagnostic tools,and therapeutic solutions that enhance patient care and improve health outcomes.It allows them to develop technologies and systems that directly contribute to diagnosing,treating and preventing diseases.
基金supported by the Grant PID2021-126715OB-IOO financed by MCIN/AEI/10.13039/501100011033 and"ERDFA way of making Europe"by the Grant PI22CⅢ/00055 funded by Instituto de Salud CarlosⅢ(ISCⅢ)+6 种基金the UFIECPY 398/19(PEJ2018-004965) grant to RGS funded by AEI(Spain)the UFIECPY-396/19(PEJ2018-004961)grant financed by MCIN (Spain)FI23CⅢ/00003 grant funded by ISCⅢ-PFIS Spain) to PMMthe UFIECPY 328/22 (PEJ-2021-TL/BMD-21001) grant to LM financed by CAM (Spain)the grant by CAPES (Coordination for the Improvement of Higher Education Personnel)through the PDSE program (Programa de Doutorado Sanduiche no Exterior)to VSCG financed by MEC (Brazil)
文摘The brain is the most complex human organ,and commonly used models,such as two-dimensional-cell cultures and animal brains,often lack the sophistication needed to accurately use in research.In this context,human cerebral organoids have emerged as valuable tools offering a more complex,versatile,and human-relevant system than traditional animal models,which are often unable to replicate the intricate architecture and functionality of the human brain.Since human cerebral organoids are a state-of-the-art model for the study of neurodevelopment and different pathologies affecting the brain,this field is currently under constant development,and work in this area is abundant.In this review,we give a complete overview of human cerebral organoids technology,starting from the different types of protocols that exist to generate different human cerebral organoids.We continue with the use of brain organoids for the study of brain pathologies,highlighting neurodevelopmental,psychiatric,neurodegenerative,brain tumor,and infectious diseases.Because of the potential value of human cerebral organoids,we describe their use in transplantation,drug screening,and toxicology assays.We also discuss the technologies available to study cell diversity and physiological characteristics of organoids.Finally,we summarize the limitations that currently exist in the field,such as the development of vasculature and microglia,and highlight some of the novel approaches being pursued through bioengineering.
