Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell via...Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell viability in the consequent fermentation step to compete the fermentable sugars with fermenting strains,resulting in sugar loss and reduced target product yield.This study investigated the competitive growth property between the biodetoxification fungus Paecilomyces variotii FN89 and the L-lactic acid bacterium Pediococcus acidilactici ZY271 under varying temperature and lactic acid osmatic stress.The results show that the L-lactic acid bacterium Ped.acidilactici ZY271 showed less thermotolerance to Pae.variotii FN89 at high temperature of 45°C to 50°C in both synthetic medium and wheat straw hydrolysate.In the higher temperature environment,the growth of the biodetoxification strian failed to compete with the lactic acid fermentation strain and was quickly eliminated from the fermentation system.The high temperature fermentation facilitated a fast transition from the detoxification stage to the fermentation stage for higher production of L-lactic acid.展开更多
Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process.Paecilomyces variotii FN89,a recently isolated mesophilic f...Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process.Paecilomyces variotii FN89,a recently isolated mesophilic filamentous fungi,was herein shown to be able to biodegrade lignocellulose-derived inhibitors including furfural(1.5 g/L),5-hydroxymethylfurfural(4 g/L),acetic acid(4 g/L),hydroxybenzaldehyde(0.2 g/L),syringaldehyde(0.2 g/L),and vanillin(1.5 g/L)efficiently and completely.P.variotii FN89 was adapted to mixed inhibitors and relatively low dissolved oxygen conditions,which can detoxify both the highly viscosity hydrolysate and solid biomass with the well preserve of fermentable sugars and no addition of any nutrients.Two biorefinery chains involving biodetoxification process were thus established to cope with different forms of pretreated biomass for cellulosic lactic acid production.The cellulosic lactic acid titers were above 100 g/L from 25%(w/w)solids loading pretreated wheat straw.The global transcriptome analysis of P.variotii FN89 in the presence of mixed inhibitors suggested that the glycolysis pathway and pentose phosphate pathway were repressed while tricarboxylic acid cycle was enhanced,ensuring the complete degradation of the inhibitors-derived intermediates and efficient energy supply.This study provided a unique and practical biodetoxification strain for lignocellulosic biorefinery,as well as enriched the knowledge of the molecular basis of lignocellulose-derived inhibitors tolerance and carbohydrates metabolism of P.variotii.展开更多
基金The National Key R&D Program of China(2023YFA0914400)the National Natural Science Foundation of China(32301269)+1 种基金China Postdoctoral Science Foundation(2023M741175)the Yangfan Project of Science and Technology Committee of Shanghai Municipality(23YF1409900).
文摘Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell viability in the consequent fermentation step to compete the fermentable sugars with fermenting strains,resulting in sugar loss and reduced target product yield.This study investigated the competitive growth property between the biodetoxification fungus Paecilomyces variotii FN89 and the L-lactic acid bacterium Pediococcus acidilactici ZY271 under varying temperature and lactic acid osmatic stress.The results show that the L-lactic acid bacterium Ped.acidilactici ZY271 showed less thermotolerance to Pae.variotii FN89 at high temperature of 45°C to 50°C in both synthetic medium and wheat straw hydrolysate.In the higher temperature environment,the growth of the biodetoxification strian failed to compete with the lactic acid fermentation strain and was quickly eliminated from the fermentation system.The high temperature fermentation facilitated a fast transition from the detoxification stage to the fermentation stage for higher production of L-lactic acid.
基金supported by the National Key R&D Program of China(2023YFA0914400)the National Natural Science Foundation of China(32301269)+1 种基金the Yangfan Project of Science and Technology Committee of Shanghai Municipality(23YF1409900)the Key Technology R&D Program of the Science and Technology Commission of Shanghai Municipality(25HC2820200).
文摘Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process.Paecilomyces variotii FN89,a recently isolated mesophilic filamentous fungi,was herein shown to be able to biodegrade lignocellulose-derived inhibitors including furfural(1.5 g/L),5-hydroxymethylfurfural(4 g/L),acetic acid(4 g/L),hydroxybenzaldehyde(0.2 g/L),syringaldehyde(0.2 g/L),and vanillin(1.5 g/L)efficiently and completely.P.variotii FN89 was adapted to mixed inhibitors and relatively low dissolved oxygen conditions,which can detoxify both the highly viscosity hydrolysate and solid biomass with the well preserve of fermentable sugars and no addition of any nutrients.Two biorefinery chains involving biodetoxification process were thus established to cope with different forms of pretreated biomass for cellulosic lactic acid production.The cellulosic lactic acid titers were above 100 g/L from 25%(w/w)solids loading pretreated wheat straw.The global transcriptome analysis of P.variotii FN89 in the presence of mixed inhibitors suggested that the glycolysis pathway and pentose phosphate pathway were repressed while tricarboxylic acid cycle was enhanced,ensuring the complete degradation of the inhibitors-derived intermediates and efficient energy supply.This study provided a unique and practical biodetoxification strain for lignocellulosic biorefinery,as well as enriched the knowledge of the molecular basis of lignocellulose-derived inhibitors tolerance and carbohydrates metabolism of P.variotii.
