The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of ...The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of grazing group suffered miscarriage or premature birth,compared with the captive group ewes without this phenomenon. Blood test result shows that the free gossypol concentration in serum of grazing group was significantly higher than in indoor captive feeding group( P < 0. 01). In order to ensure the health of sheep grazing on cotton stubble and improve breeding rate,there is a need to do further reasonable research of grazing and feeding methods.展开更多
Biofilms are being engineered in-vitro to produce numerous commodities like biofertilizers, pharmaceuticals, biofuels and electricity, the efficacies of which rely on the biochemicals secreted by the biofilms i.e. ext...Biofilms are being engineered in-vitro to produce numerous commodities like biofertilizers, pharmaceuticals, biofuels and electricity, the efficacies of which rely on the biochemicals secreted by the biofilms i.e. extracellular polymeric substances (EPS). It has been shown that once EPS-biochemicals of developed biofilms are applied to an ecosystem, they can restore degraded complex ecosystem networks for improved ecosystem functioning and sustainability. Identification of the EPS biochemicals and understanding their contributions to the network interactions in particular, are at initial stage. In the present study, using Aspergillus niger, Nostoc sp., and gram (-) Stenotrophomonas maltophilia & gram (+) Bacillus subtilis as test fungal (F), cyanobacterial (C), and bacterial (B) counterparts, respectively we analyzed morphology and biochemical parameters of fungal-bacterial (FBBs), fungal-cyanobacterial (FCBs), cyanobacterial-bacterial (CBBs), and fungal-cyanobacterial-bacterial biofilms (FCBBs). Results revealed that the FCBBs produced the highest concentrations of lipids, proteins, and polysaccharides whereas FBBs generated the highest diversity of biochemicals. Bacterial type (i.e. gram + or -) and microbial composition in the biofilm affected the biochemical production. Ecologically and industrially important diverse biochemicals which are used individually as medicines, bioremediating agents and industrial chemicals in human society with certain adverse and beneficial effects were detected in the biofilm-EPS. However, in the nature, simultaneous action of those diverse biochemicals applied as biofertilizers has already shown a huge potential to restore the entire agroecosystems degraded due to farmers’ detrimental practices. This striking difference in utilization of the biochemicals and their enhanced effect when they act simultaneously needs further investigations for their better applications.展开更多
Responsive photonic crystals(RPCs) constructed by periodic two/three-dimensional(2D/3D) photonic crystals(PCs) and responsive-material hosts,are important visualized optical sensors.Their optical diffraction color can...Responsive photonic crystals(RPCs) constructed by periodic two/three-dimensional(2D/3D) photonic crystals(PCs) and responsive-material hosts,are important visualized optical sensors.Their optical diffraction color can be tuned reversibly by external stimuli,such as pH,metal ions,biomolecules,vapors and solvents,hence leading to wide applications as visualized sensors.This review introduces the recent progress of RPCs based on 2D/3D PCs for visual detection of chemical and biological analytes,including the preparation of 2D PCs,3D PCs films,3D PCs microbeads and their applications as visualized sensors.The different cases of detecting various chemical and biological analytes by naked eyes are presented.Emphasis is given to the description of their respective sensing mechanisms with the different systems for chemical and biological analytes.Compared with 3D RPCs sensors,2D RPCs sensors have shorter response time,better stabilization and higher production efficiency,however,the diffraction intensity of 2D RPCs based on monolayered 2D polystyrene(PS) microsphere array are weak.2D RPCs sensors based on 2D Au nanosphere can significantly improve the diffraction intensity compared with traditional 2D RPCs sensors based on monolayered PS microsphere array.The much higher scattering cross section of Au nanosphere leads to 2D Au nanosphere array with ultrahigh optical diffraction intensity,which are highly helpful for their practical application as visual sensors and further quantitative detection by monitoring the diffraction peak position and intensity.展开更多
Biofuels and bio-based chemicals are getting more and more attention because of their sustainable and renewable properties and wide industrial applications.However,the low concentrations of the targeted products in th...Biofuels and bio-based chemicals are getting more and more attention because of their sustainable and renewable properties and wide industrial applications.However,the low concentrations of the targeted products in their fermentation broths,the complicated components of the broths and the high energy-intensive separation and purification process hinder the competitiveness of biofuels and biochemicals with the petro-based ones.Hence,the production and the separation of biofuels and bio-based chemicals in energy-saving,low-cost and greenness ways become hot topics nowadays.This review introduces the separation technologies(salting-out extraction,salting-out,sugaring-out extraction,and sugaring-out)that extract biobutanol,1,3-propanediol,2,3-butanediol,acetoin,organic acids and other bio-based chemicals from fermentation broths/aqueous solutions.Salting-out/sugar-ing-out extraction and salting-out/sugaring-out technologies display the high separating efficiency and the high targeted product yields.In addition,they are easy to operate and require low cost for separating products.Hence,they are the effective and potential technologies for separating targeted products in the wide industrial applications.The successful research into the salting-out/sugaring-out and salting-out/sugaring-out extraction not only affords biofuels and biochemical but also opens a door for the development of novel separation methods.展开更多
Prostate cancer is the most common non-cutaneous cancers occurring in American men,and whilemost men with early-stage prostate cancers are cured,up to a third might manifest with biochemical recurrence(BCR)of prostate...Prostate cancer is the most common non-cutaneous cancers occurring in American men,and whilemost men with early-stage prostate cancers are cured,up to a third might manifest with biochemical recurrence(BCR)of prostate cancer.BCR is a disease entitywhich is characterized by a rising prostate-specific antigen(PSA)in the setting of a previously treated localized prostate cancerwith either surgery or radiation therapywith curativeintent.展开更多
According the importance of the stored grains and other products,it is an essential to keep them from khapra beetle,Trogoderma granarium infestation.This study determined the mortality percentage of 5th instar larvae ...According the importance of the stored grains and other products,it is an essential to keep them from khapra beetle,Trogoderma granarium infestation.This study determined the mortality percentage of 5th instar larvae of T.granarium fed on wheat seeds(25 gm)treated with different weights of silica as well as silica nanoparticles(20,40,60 and 80 mg)at different temperature(9℃,25℃,and 35℃).Study showed that using silica nanoparticles in cold temperature(9℃)was the most efficient treatment with the lowest LC_(50)(lethal concentration required to kill 50%of the population)value and caused the highest toxicity index.In contrast,the least efficient treatment(25℃)with the highest LC_(50) value and showed lowest toxicity index was using silica in normal temperature,when using silica nanoparticles,the cold temperature was the best condition followed by hot temperature(35℃)and finally the normal temperature.On the other hand,using silica in hot temperature was most effective followed by silica with cold temperature and finally silica with normal temperature.The biochemical assays revealed that the change in the experimental temperature had a nonsignificant effect on the total protein content of the larvae.The total lipids and total carbohydrates exhibited a significant increase due to hot treating.5th instar larvae of T.granarium treated with LC50 of silica at high temperature led to a nonsignificant(p≤0.05)decrease in Acetylcholinesterase(AchE)activity compared to treatment at normal temperature.In contrast,Glutathione S-transferase(GST)and Peroxidase activities were significantly(p≤0.05)raised due to the treatment conducted at high temperature.Additionally,treating larvae with LC50 of silica nanoparticles at low temperature caused a significant increase in both GST and peroxidase activities,while the increase in AChE was nonsignificantly(p≤0.05)compared to treatment at normal temperature.Using silica at low temperature could be used as an alternative to chemical insecticides to control T.granarium larvae.展开更多
The epizoic diatoms,especifically those living at the body surface of copepods has not garnered sufficient attention.This study focuses on the copepod Pseudodiaptomus annandalei and three epizoic diatom species isolat...The epizoic diatoms,especifically those living at the body surface of copepods has not garnered sufficient attention.This study focuses on the copepod Pseudodiaptomus annandalei and three epizoic diatom species isolated from its body surface:Pseudofalcula hyalina,Haslea sp.,and Tabularia sp.The research explored the physiological and biochemical characteristics of diatoms under co cultivation conditions with P.annandalei and different species of epizoic diatoms,along with variations in copepod survival rates.Results reveal that the growth rate,cell density,and chlorophyll-a content of the three epizoic diatoms were enhanced by P.annandalei.The photosynthesis of epizoic diatoms,particularly the photochemical efficiency of PSⅡ(F_(v)/F_(m))of P.hyalina was positively influenced by P.annandalei.The impact of P.annandalei on biochemical compositions exhibited species-specific characteristics.Under co-culture conditions,the content of polysaccharides and proteins in the colloidal exopolymeric substance(CEPS)of P.hyalina and Haslea sp.increased,so did the intracellular protein content of Tabularia sp.The attachment of P.hyalina was most significantly influenced by P.annandalei,followed by Haslea sp.,and then Tabularia sp.,likely due to the increased uronic acid content of P.hyalina under co-culture conditions.Moreover,the three epizoic diatoms might have served as food sources for P.annandalei,as evidenced by the higher survival of copepods.The release of nitrate and phosphate from P.annandalei contributed to the nutrition for the growth of epizoic diatoms,indicating that the bottom-up effect resulting from P.annandalei excretion nutrition was greater than the top-down effect from predation.展开更多
The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profil...The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profile responds and influences nutrient pool(carbon(C),nitrogen(N),phosphorus(P)and sulfur(S))in polluted water caused by animal carcass decomposition had not been explored.Here,we combined metagenomic analysis,16S rRNA gene sequencing and water physicochemical assessment to explore the response of viral communities under different temperatures(23℃,26℃,29℃,32℃,and 35℃)in water polluted by cadaver,as well as compare the contribution of viral/bacterial communities on water nutrient pool.We found that a total of 15,240 viral species were classified and mainly consisted of Siphoviridae.Both temperature and carrion reduced the viral diversity and abundance.Only a small portion of the viruses(∼8.8%)had significant negative correlations with temperature,while most were not sensitive.Our results revealed that the viruses had lager contribution on nutrient pool than bacteria.Besides,viral-related functional genes involved in C,N,P and S cycling.These functional genes declined during carcass decomposition and covered part of the central nutrient cycle metabolism(including carbon sugar transformation,denitrification,P mineralization and extracelluar sulfate transfer,etc.).Our result implies that human regulation of virus communities may be more important than bacterial communities in regulating and managing polluted water quality and nutrition.展开更多
This present study identifies endophytic bacteria from Linum usitatissimum with multidimensional plant growth-promoting attributes,positioning them as ecological engineers for sustainable agriculture.Plant growthpromo...This present study identifies endophytic bacteria from Linum usitatissimum with multidimensional plant growth-promoting attributes,positioning them as ecological engineers for sustainable agriculture.Plant growthpromoting bacteria(PGPB)are present in symbiotic associations with plants or rhizosphere.These microbes enhance crop productivity and resilience under different environmental conditions.Endophytes are a type of PGPB that inhabit inside plant tissues and contribute to plant growth by phytohormone production,phosphate solubilisation,zinc solubilisation,siderophore production,ammonia production,nitrogen fixation,stress tolerance,and biocontrol mechanisms.Twelve bacterial strains were isolated from Linum usitatissimum exhibiting plant growth-promoting attributes such as ammonia and indole-3-acetic acid(IAA)production,siderophore synthesis,phosphate solubilisation,and extracellular enzyme synthesis.The isolated endophytes were also assessed for different enzymatic activities such as;cellulase,pectinase,xylanase,amylase,and gelatinase,which contribute to development of a symbiotic relationship and are crucial for the degradation of plant cell wall components The most efficient endophytes identified in the present study were Pseudomonas sp.strain JL-1(ESL1)and Staphylococcus sciuri(ESL2),both of which displayed strong plant growth-promoting potential.ESL1 and ESL2 demonstrated promising plant growth-promoting characteristics and cellulase,pectinase,xylanase,amylase,and gelatinase,activity.ESL2(Staphylococcus sciuri)enhanced nutrient cycling(phosphate solubilisation:196-209μg/ml;siderophores:68-71%)and stress tolerance(IAA:11-12μg/ml),reducing reliance on synthetic inputs.By integrating flax microbiomes into agro-ecosystems,we demonstrate a scalable approach to reconcile crop productivity with soil biodiversity conservation.These results demonstrate the potentiality of these endophytic microbes in sustainable agriculture,environmental management,and microbial biotechnology.Further studies on their metabolic pathways may expand their applications in bioremediation and plant-microbe interactions.展开更多
The co-production of hydrogen and value-added biochemicals from lignocellulose utilizing solar energy has been regarded as one of the technologies most potentially able to alleviate the current energy crisis.Here,we d...The co-production of hydrogen and value-added biochemicals from lignocellulose utilizing solar energy has been regarded as one of the technologies most potentially able to alleviate the current energy crisis.Here,we demonstrate a cost-effective photoreforming strategy for lignocellulose valorization using a carbon nitride-supported platinum single-atom photocatalyst.An advanced H_(2) evolution rate of 6.34 mmol molPt^(-1) h^(-1) is achieved over the optimal catalyst,which is around 4.6 and 30.5 times higher compared with the nanosized Pt counterpart and pristine carbon nitride,respectively.Meanwhile,the monosaccharides are oxidized to value-added lactic acid with>99%conversion and extraordinary selectivity up to 97%.The theoretical calculations show that with Pt incorporation,the photogenerated holes are predominantly localized on the metal sites while the photogenerated electrons are concentrated on C_(3)N_(4),thus enhancing the effective separation of charge carriers.This work provides a promising avenue for the simultaneous production of green H2 and bio-based chemicals by biomass photorefinery.展开更多
This letter critiques the article by Xu et al in World Journal of Psychiatry,which developed a nomogram to predict cognitive impairment in elderly hypertensive patients using nutritional and biochemical parameters.Whi...This letter critiques the article by Xu et al in World Journal of Psychiatry,which developed a nomogram to predict cognitive impairment in elderly hypertensive patients using nutritional and biochemical parameters.While the model's use of variables like body mass index,albumin,hemoglobin,alkaline phosphatase,and mini-nutritional assessment scores is promising,we raise concerns about the small validation cohort size,potential reverse causality in cross-sectional data,insufficiently discussed mechanisms for alkaline phosphatase as a risk factor,omission of key cognitive predictors,and possible overfitting given high area under the curve values.We suggest external validation,longitudinal studies,and calibration metrics to enhance the model's robustness and clinical utility.展开更多
Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various in...Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various investigations. This cross-sectional study, conducted in Cotonou among 35 azoospermic men, aimed to assess the role of biochemical analysis of seminal fluid in determining the etiological diagnosis. The results revealed significant heterogeneity in biochemical profiles. Most of the patients had normal levels of alpha-glucosidase, fructose, and zinc, indicating normal accessory gland function and suggesting non-obstructive azoospermia. However, a significant subgroup exhibited abnormalities in these markers, suggesting a possible obstructive azoospermia. The heterogeneity in biochemical profiles observed in this study highlights the need for further studies to better characterize azoospermia in Benin.展开更多
BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinica...BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinical treatment decisions.Currently,there is no established set of reference intervals for cord blood biochemical parameters of newborns in India,particularly in Mumbai.AIM To create cord blood biochemical parameters reference intervals specifically for Mumbai,India.METHODS A cross-sectional study was conducted in an Indian tertiary care hospital.This study focused on healthy newborns with normal birth weight,born to pregnant mothers without health issues.Cord blood samples,approximately 2-3 mL in volume,were collected from 210 term neonates.These samples were divided into fluoride(glucose)and clot activator(serum)tubes and were subsequently analyzed in the institute's biochemical laboratory.The data obtained from the analysis was then subjected to statistical analysis.The result of the Shapiro-Wilk test suggested non-normality in the data distribution.Consequently,nonparametric statistics were utilized for analysis.The Mann-Whitney U test was utilized to compare parameter distributions among different factors,including the infant’s sex,delivery method,maternal age,and obstetric history.A significance level of P<0.05 was considered to indicate statistical significance.RESULTS The following represent the median figures and central 95 percentile reference intervals for biochemical parameters in umbilical cord blood of newborns:Serum direct bilirubin=(0.1-0.55)mg/dL,indirect bilirubin=(0.64-2.26)mg/dL,total bilirubin=(0.62-3.14)mg/dL,creatinine=(0.27-0.76)mg/dL,sodium=(128.19-143.26)mmol/L,chloride=(100.19-111.68)mmol/L,potassium=(1.62-9.98)mmol/L and plasma glucose=(24.75-94.23)mg/dL.Statistically significant differences were observed in serum sodium,potassium,and plasma glucose levels when comparing delivery modes.CONCLUSION This is the pioneering study in which first time,the biochemical reference intervals in cord blood for newborns are established in western India.The values are applicable for newborns from this area.Larger study throughout the country is required.展开更多
A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the...A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the water in Lake Fitri in Chad. Standard microbiology methods were used. Isolated strains of enterobacteria were characterized by API 20E and API Staph galleries and confirmed by API Web. Antibiotic resistance was performed according to the recommendations of the antibiogram committee (CA-SFM, 2019). Physico-chemical analysis of the water was carried out by (Rodier, 2009). The microbiological results revealed an abundance on the Domoro islands (5.37.106 ± 1.5.105 and 1.97.105 ± 4.94.104), successively for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli) and on the Maguite Islands (4.71.106 ± 7.14.105 and 2.32.105 ± 2.86.104), alternately for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli). The results obtained after incubation of the biochemical tests using the API 20E and API Staph galleries and their proposed numerical profile analyzed using Api software and confirmed by Api Web confirmed the contamination of the waters of Lake Fitri by pathogenic strains of E. coli, Staphylococcus and Salmonella spp. The results of the antibiogram carried out show the emergence of certain resistances to Tobramycin, Flucytosine and Teicoplanin. The average levels of BOD5, COD, SS, Nitrate (NO3) and Nitrite (NO2) for Domoro Island were 32.14 ± 2.37 mg/L, 423.86 ± 4.78 mg/L, 65.42 ± 2.27 mg/L, 0.04 ± 0.01 mg/L and 3.32 ± 1.48 mg/L respectively. The water from the islands of Lake Fitri must therefore be rigorously treated before consumption.展开更多
The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due ...The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.展开更多
1 Plant SecondaryMetabolism and Functional Biology Progress Plants have long been recognized as biochemical powerhouses,producing a vast array of compounds through their secondary metabolic pathways[1].Although histor...1 Plant SecondaryMetabolism and Functional Biology Progress Plants have long been recognized as biochemical powerhouses,producing a vast array of compounds through their secondary metabolic pathways[1].Although historically referred to as‘secondary’due to their perceived non-essential role in basic plant survival,it is now understood that these metabolites are integral to plant growth,development and adaptation to environmental challenges.Secondary metabolites,such as alkaloids,terpenoids,phenolics and flavonoids,serve as critical mediators of plant-environment interactions,conferring resistance to biotic and abiotic stressors[2].Beyond their ecological functions,these compounds are invaluable to humans,supporting industries ranging from pharmaceuticals and nutrition to agrochemicals and chemical additives.展开更多
A spice formulation study in Burkina Faso was carried out using local ingredients for the benefit of households. The objective of this study was to propose some spice formulations based on local ingredients in order t...A spice formulation study in Burkina Faso was carried out using local ingredients for the benefit of households. The objective of this study was to propose some spice formulations based on local ingredients in order to reduce the use of chemical spices in the preparation of different dishes. The Design of Experiments (DOE) methodology was used for the formulation of the spices and their physicochemical, nutritional and sensory characteristics were evaluated by standardized and standard methods. The results obtained showed lipid contents (g/100 g DM) ranging from 10.41 ± 0.26 to 15.64 ± 0.68, total sugars from 4.39 ± 0.32 to 5.46 ± 0.31, protein from 3.65 ± 0.17 to 12.04 ± 0.35 and ash from 5.83 ± 0.01 to 7.02 ± 0.01. The polyphenol content ranged from 9.09 ± 1.60 to 11.33 ± 0.90, and the flavonoid content ranged from 0.65 ± 0.03 to 1.08 ± 0.13. The sensory analysis carried out showed that the spices have generally satisfactory organoleptic characteristics. These results constitute new information in the diet of populations and are an alternative to the chemical spices used in their cooking.展开更多
At the beginning of the 20^(th)century,German scientist Richard Semon introduced the term'engram'to describe the neural substrate implicated in the processes of memory formation and retrieval[1].The trace of t...At the beginning of the 20^(th)century,German scientist Richard Semon introduced the term'engram'to describe the neural substrate implicated in the processes of memory formation and retrieval[1].The trace of the corresponding biophysical and biochemical changes in the brain responding to an external stimulus is called an engram,and understanding the physical manifestations of memory formation and recall remains a fundamental yet unresolved question[2].展开更多
Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field face...Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field faces considerable challenges,including the continuous emergence of potential health threats,as well as novel pathogen strains and viruses.The advent and implementation of advanced technologies,such as culture strategies,genomics mining,and artificial intelligence(AI),are facilitating a paradigm shift in pharmaceutical research,introducing innovative methodologies and perspectives.The development and maturation of these technologies have enhanced the exploration of microbial-derived NPs,thereby advancing pharmaceutical research and development.This review synthesizes recent developments in this context,emphasizing their applications in pharmaceutical discovery and development.Through systematic analysis and synthesis,it provides objective insights into the promising prospects and future direction of this essential field.展开更多
The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and wh...The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and white mustard meals as fish feed ingredients for Indian Major Carps.Fish were fed with 10%mustard meal-supplemented diets in three forms:Raw(R),Anti-nutritional Rich(AR),and Anti-nutritional Lowered(AL),alongside a control group using floating feed.The three-month indoor experiment(September-November 2023)was conducted in FRP tanks with triplicate treatments.Blood analysis revealed compromised health in AR-fed carps,with reduced hemoglobin levels in rohu,catla and mrigal and elevated total leukocyte counts indicating inflammation in all the three carps studied here.Liver function was impaired in AR-fed fish,shown by increased alanine transaminase levels,highest in rohu followed by mrigal and catla.Histopathological examination of AR-fed carps liver tissue revealed necrotic spots,deformed hepatocytes,and significant vacuolation.In contrast,AL-fed fish demonstrated improved health parameters through Complete Blood Count analysis,liver function tests,and histo-pathological observations,suggesting successful reduction of anti-nutritional factors in the processed mustard meals.In near future,replacement of unprocessed seed meal with processed seed meal will lead to economic gains in fish farming.展开更多
基金Supported by National Natural Science Foundation of China(31360565)
文摘The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of grazing group suffered miscarriage or premature birth,compared with the captive group ewes without this phenomenon. Blood test result shows that the free gossypol concentration in serum of grazing group was significantly higher than in indoor captive feeding group( P < 0. 01). In order to ensure the health of sheep grazing on cotton stubble and improve breeding rate,there is a need to do further reasonable research of grazing and feeding methods.
文摘Biofilms are being engineered in-vitro to produce numerous commodities like biofertilizers, pharmaceuticals, biofuels and electricity, the efficacies of which rely on the biochemicals secreted by the biofilms i.e. extracellular polymeric substances (EPS). It has been shown that once EPS-biochemicals of developed biofilms are applied to an ecosystem, they can restore degraded complex ecosystem networks for improved ecosystem functioning and sustainability. Identification of the EPS biochemicals and understanding their contributions to the network interactions in particular, are at initial stage. In the present study, using Aspergillus niger, Nostoc sp., and gram (-) Stenotrophomonas maltophilia & gram (+) Bacillus subtilis as test fungal (F), cyanobacterial (C), and bacterial (B) counterparts, respectively we analyzed morphology and biochemical parameters of fungal-bacterial (FBBs), fungal-cyanobacterial (FCBs), cyanobacterial-bacterial (CBBs), and fungal-cyanobacterial-bacterial biofilms (FCBBs). Results revealed that the FCBBs produced the highest concentrations of lipids, proteins, and polysaccharides whereas FBBs generated the highest diversity of biochemicals. Bacterial type (i.e. gram + or -) and microbial composition in the biofilm affected the biochemical production. Ecologically and industrially important diverse biochemicals which are used individually as medicines, bioremediating agents and industrial chemicals in human society with certain adverse and beneficial effects were detected in the biofilm-EPS. However, in the nature, simultaneous action of those diverse biochemicals applied as biofertilizers has already shown a huge potential to restore the entire agroecosystems degraded due to farmers’ detrimental practices. This striking difference in utilization of the biochemicals and their enhanced effect when they act simultaneously needs further investigations for their better applications.
基金supported by the National Natural Science Foundation of China(51371165,51571189)the Anhui Provincial Natural Science Foundation(1508085JGD07)+1 种基金the Crossdisciplinary Collaborative Teams Program in CASthe CAS/ SAFEA International Partnership Program for Creative Research Teams
文摘Responsive photonic crystals(RPCs) constructed by periodic two/three-dimensional(2D/3D) photonic crystals(PCs) and responsive-material hosts,are important visualized optical sensors.Their optical diffraction color can be tuned reversibly by external stimuli,such as pH,metal ions,biomolecules,vapors and solvents,hence leading to wide applications as visualized sensors.This review introduces the recent progress of RPCs based on 2D/3D PCs for visual detection of chemical and biological analytes,including the preparation of 2D PCs,3D PCs films,3D PCs microbeads and their applications as visualized sensors.The different cases of detecting various chemical and biological analytes by naked eyes are presented.Emphasis is given to the description of their respective sensing mechanisms with the different systems for chemical and biological analytes.Compared with 3D RPCs sensors,2D RPCs sensors have shorter response time,better stabilization and higher production efficiency,however,the diffraction intensity of 2D RPCs based on monolayered 2D polystyrene(PS) microsphere array are weak.2D RPCs sensors based on 2D Au nanosphere can significantly improve the diffraction intensity compared with traditional 2D RPCs sensors based on monolayered PS microsphere array.The much higher scattering cross section of Au nanosphere leads to 2D Au nanosphere array with ultrahigh optical diffraction intensity,which are highly helpful for their practical application as visual sensors and further quantitative detection by monitoring the diffraction peak position and intensity.
文摘Biofuels and bio-based chemicals are getting more and more attention because of their sustainable and renewable properties and wide industrial applications.However,the low concentrations of the targeted products in their fermentation broths,the complicated components of the broths and the high energy-intensive separation and purification process hinder the competitiveness of biofuels and biochemicals with the petro-based ones.Hence,the production and the separation of biofuels and bio-based chemicals in energy-saving,low-cost and greenness ways become hot topics nowadays.This review introduces the separation technologies(salting-out extraction,salting-out,sugaring-out extraction,and sugaring-out)that extract biobutanol,1,3-propanediol,2,3-butanediol,acetoin,organic acids and other bio-based chemicals from fermentation broths/aqueous solutions.Salting-out/sugar-ing-out extraction and salting-out/sugaring-out technologies display the high separating efficiency and the high targeted product yields.In addition,they are easy to operate and require low cost for separating products.Hence,they are the effective and potential technologies for separating targeted products in the wide industrial applications.The successful research into the salting-out/sugaring-out and salting-out/sugaring-out extraction not only affords biofuels and biochemical but also opens a door for the development of novel separation methods.
文摘Prostate cancer is the most common non-cutaneous cancers occurring in American men,and whilemost men with early-stage prostate cancers are cured,up to a third might manifest with biochemical recurrence(BCR)of prostate cancer.BCR is a disease entitywhich is characterized by a rising prostate-specific antigen(PSA)in the setting of a previously treated localized prostate cancerwith either surgery or radiation therapywith curativeintent.
文摘According the importance of the stored grains and other products,it is an essential to keep them from khapra beetle,Trogoderma granarium infestation.This study determined the mortality percentage of 5th instar larvae of T.granarium fed on wheat seeds(25 gm)treated with different weights of silica as well as silica nanoparticles(20,40,60 and 80 mg)at different temperature(9℃,25℃,and 35℃).Study showed that using silica nanoparticles in cold temperature(9℃)was the most efficient treatment with the lowest LC_(50)(lethal concentration required to kill 50%of the population)value and caused the highest toxicity index.In contrast,the least efficient treatment(25℃)with the highest LC_(50) value and showed lowest toxicity index was using silica in normal temperature,when using silica nanoparticles,the cold temperature was the best condition followed by hot temperature(35℃)and finally the normal temperature.On the other hand,using silica in hot temperature was most effective followed by silica with cold temperature and finally silica with normal temperature.The biochemical assays revealed that the change in the experimental temperature had a nonsignificant effect on the total protein content of the larvae.The total lipids and total carbohydrates exhibited a significant increase due to hot treating.5th instar larvae of T.granarium treated with LC50 of silica at high temperature led to a nonsignificant(p≤0.05)decrease in Acetylcholinesterase(AchE)activity compared to treatment at normal temperature.In contrast,Glutathione S-transferase(GST)and Peroxidase activities were significantly(p≤0.05)raised due to the treatment conducted at high temperature.Additionally,treating larvae with LC50 of silica nanoparticles at low temperature caused a significant increase in both GST and peroxidase activities,while the increase in AChE was nonsignificantly(p≤0.05)compared to treatment at normal temperature.Using silica at low temperature could be used as an alternative to chemical insecticides to control T.granarium larvae.
基金Supported by the National Natural Science Foundation of China(No.42076114)the Second Batch of High-level Professional Groups of Vocational Colleges in Guangdong Province(No.GSPZYQ 2021004)the Guangxi Science and Technology Base and Talent Special Project(No.GUIKE AD 23026041)。
文摘The epizoic diatoms,especifically those living at the body surface of copepods has not garnered sufficient attention.This study focuses on the copepod Pseudodiaptomus annandalei and three epizoic diatom species isolated from its body surface:Pseudofalcula hyalina,Haslea sp.,and Tabularia sp.The research explored the physiological and biochemical characteristics of diatoms under co cultivation conditions with P.annandalei and different species of epizoic diatoms,along with variations in copepod survival rates.Results reveal that the growth rate,cell density,and chlorophyll-a content of the three epizoic diatoms were enhanced by P.annandalei.The photosynthesis of epizoic diatoms,particularly the photochemical efficiency of PSⅡ(F_(v)/F_(m))of P.hyalina was positively influenced by P.annandalei.The impact of P.annandalei on biochemical compositions exhibited species-specific characteristics.Under co-culture conditions,the content of polysaccharides and proteins in the colloidal exopolymeric substance(CEPS)of P.hyalina and Haslea sp.increased,so did the intracellular protein content of Tabularia sp.The attachment of P.hyalina was most significantly influenced by P.annandalei,followed by Haslea sp.,and then Tabularia sp.,likely due to the increased uronic acid content of P.hyalina under co-culture conditions.Moreover,the three epizoic diatoms might have served as food sources for P.annandalei,as evidenced by the higher survival of copepods.The release of nitrate and phosphate from P.annandalei contributed to the nutrition for the growth of epizoic diatoms,indicating that the bottom-up effect resulting from P.annandalei excretion nutrition was greater than the top-down effect from predation.
基金supported by the National Natural Science Foundation of China (No.42007026)the Medical Innovation and Development Project of Lanzhou University (lzuyxcx-2022-172)
文摘The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profile responds and influences nutrient pool(carbon(C),nitrogen(N),phosphorus(P)and sulfur(S))in polluted water caused by animal carcass decomposition had not been explored.Here,we combined metagenomic analysis,16S rRNA gene sequencing and water physicochemical assessment to explore the response of viral communities under different temperatures(23℃,26℃,29℃,32℃,and 35℃)in water polluted by cadaver,as well as compare the contribution of viral/bacterial communities on water nutrient pool.We found that a total of 15,240 viral species were classified and mainly consisted of Siphoviridae.Both temperature and carrion reduced the viral diversity and abundance.Only a small portion of the viruses(∼8.8%)had significant negative correlations with temperature,while most were not sensitive.Our results revealed that the viruses had lager contribution on nutrient pool than bacteria.Besides,viral-related functional genes involved in C,N,P and S cycling.These functional genes declined during carcass decomposition and covered part of the central nutrient cycle metabolism(including carbon sugar transformation,denitrification,P mineralization and extracelluar sulfate transfer,etc.).Our result implies that human regulation of virus communities may be more important than bacterial communities in regulating and managing polluted water quality and nutrition.
文摘This present study identifies endophytic bacteria from Linum usitatissimum with multidimensional plant growth-promoting attributes,positioning them as ecological engineers for sustainable agriculture.Plant growthpromoting bacteria(PGPB)are present in symbiotic associations with plants or rhizosphere.These microbes enhance crop productivity and resilience under different environmental conditions.Endophytes are a type of PGPB that inhabit inside plant tissues and contribute to plant growth by phytohormone production,phosphate solubilisation,zinc solubilisation,siderophore production,ammonia production,nitrogen fixation,stress tolerance,and biocontrol mechanisms.Twelve bacterial strains were isolated from Linum usitatissimum exhibiting plant growth-promoting attributes such as ammonia and indole-3-acetic acid(IAA)production,siderophore synthesis,phosphate solubilisation,and extracellular enzyme synthesis.The isolated endophytes were also assessed for different enzymatic activities such as;cellulase,pectinase,xylanase,amylase,and gelatinase,which contribute to development of a symbiotic relationship and are crucial for the degradation of plant cell wall components The most efficient endophytes identified in the present study were Pseudomonas sp.strain JL-1(ESL1)and Staphylococcus sciuri(ESL2),both of which displayed strong plant growth-promoting potential.ESL1 and ESL2 demonstrated promising plant growth-promoting characteristics and cellulase,pectinase,xylanase,amylase,and gelatinase,activity.ESL2(Staphylococcus sciuri)enhanced nutrient cycling(phosphate solubilisation:196-209μg/ml;siderophores:68-71%)and stress tolerance(IAA:11-12μg/ml),reducing reliance on synthetic inputs.By integrating flax microbiomes into agro-ecosystems,we demonstrate a scalable approach to reconcile crop productivity with soil biodiversity conservation.These results demonstrate the potentiality of these endophytic microbes in sustainable agriculture,environmental management,and microbial biotechnology.Further studies on their metabolic pathways may expand their applications in bioremediation and plant-microbe interactions.
文摘The co-production of hydrogen and value-added biochemicals from lignocellulose utilizing solar energy has been regarded as one of the technologies most potentially able to alleviate the current energy crisis.Here,we demonstrate a cost-effective photoreforming strategy for lignocellulose valorization using a carbon nitride-supported platinum single-atom photocatalyst.An advanced H_(2) evolution rate of 6.34 mmol molPt^(-1) h^(-1) is achieved over the optimal catalyst,which is around 4.6 and 30.5 times higher compared with the nanosized Pt counterpart and pristine carbon nitride,respectively.Meanwhile,the monosaccharides are oxidized to value-added lactic acid with>99%conversion and extraordinary selectivity up to 97%.The theoretical calculations show that with Pt incorporation,the photogenerated holes are predominantly localized on the metal sites while the photogenerated electrons are concentrated on C_(3)N_(4),thus enhancing the effective separation of charge carriers.This work provides a promising avenue for the simultaneous production of green H2 and bio-based chemicals by biomass photorefinery.
文摘This letter critiques the article by Xu et al in World Journal of Psychiatry,which developed a nomogram to predict cognitive impairment in elderly hypertensive patients using nutritional and biochemical parameters.While the model's use of variables like body mass index,albumin,hemoglobin,alkaline phosphatase,and mini-nutritional assessment scores is promising,we raise concerns about the small validation cohort size,potential reverse causality in cross-sectional data,insufficiently discussed mechanisms for alkaline phosphatase as a risk factor,omission of key cognitive predictors,and possible overfitting given high area under the curve values.We suggest external validation,longitudinal studies,and calibration metrics to enhance the model's robustness and clinical utility.
文摘Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various investigations. This cross-sectional study, conducted in Cotonou among 35 azoospermic men, aimed to assess the role of biochemical analysis of seminal fluid in determining the etiological diagnosis. The results revealed significant heterogeneity in biochemical profiles. Most of the patients had normal levels of alpha-glucosidase, fructose, and zinc, indicating normal accessory gland function and suggesting non-obstructive azoospermia. However, a significant subgroup exhibited abnormalities in these markers, suggesting a possible obstructive azoospermia. The heterogeneity in biochemical profiles observed in this study highlights the need for further studies to better characterize azoospermia in Benin.
文摘BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinical treatment decisions.Currently,there is no established set of reference intervals for cord blood biochemical parameters of newborns in India,particularly in Mumbai.AIM To create cord blood biochemical parameters reference intervals specifically for Mumbai,India.METHODS A cross-sectional study was conducted in an Indian tertiary care hospital.This study focused on healthy newborns with normal birth weight,born to pregnant mothers without health issues.Cord blood samples,approximately 2-3 mL in volume,were collected from 210 term neonates.These samples were divided into fluoride(glucose)and clot activator(serum)tubes and were subsequently analyzed in the institute's biochemical laboratory.The data obtained from the analysis was then subjected to statistical analysis.The result of the Shapiro-Wilk test suggested non-normality in the data distribution.Consequently,nonparametric statistics were utilized for analysis.The Mann-Whitney U test was utilized to compare parameter distributions among different factors,including the infant’s sex,delivery method,maternal age,and obstetric history.A significance level of P<0.05 was considered to indicate statistical significance.RESULTS The following represent the median figures and central 95 percentile reference intervals for biochemical parameters in umbilical cord blood of newborns:Serum direct bilirubin=(0.1-0.55)mg/dL,indirect bilirubin=(0.64-2.26)mg/dL,total bilirubin=(0.62-3.14)mg/dL,creatinine=(0.27-0.76)mg/dL,sodium=(128.19-143.26)mmol/L,chloride=(100.19-111.68)mmol/L,potassium=(1.62-9.98)mmol/L and plasma glucose=(24.75-94.23)mg/dL.Statistically significant differences were observed in serum sodium,potassium,and plasma glucose levels when comparing delivery modes.CONCLUSION This is the pioneering study in which first time,the biochemical reference intervals in cord blood for newborns are established in western India.The values are applicable for newborns from this area.Larger study throughout the country is required.
文摘A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the water in Lake Fitri in Chad. Standard microbiology methods were used. Isolated strains of enterobacteria were characterized by API 20E and API Staph galleries and confirmed by API Web. Antibiotic resistance was performed according to the recommendations of the antibiogram committee (CA-SFM, 2019). Physico-chemical analysis of the water was carried out by (Rodier, 2009). The microbiological results revealed an abundance on the Domoro islands (5.37.106 ± 1.5.105 and 1.97.105 ± 4.94.104), successively for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli) and on the Maguite Islands (4.71.106 ± 7.14.105 and 2.32.105 ± 2.86.104), alternately for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli). The results obtained after incubation of the biochemical tests using the API 20E and API Staph galleries and their proposed numerical profile analyzed using Api software and confirmed by Api Web confirmed the contamination of the waters of Lake Fitri by pathogenic strains of E. coli, Staphylococcus and Salmonella spp. The results of the antibiogram carried out show the emergence of certain resistances to Tobramycin, Flucytosine and Teicoplanin. The average levels of BOD5, COD, SS, Nitrate (NO3) and Nitrite (NO2) for Domoro Island were 32.14 ± 2.37 mg/L, 423.86 ± 4.78 mg/L, 65.42 ± 2.27 mg/L, 0.04 ± 0.01 mg/L and 3.32 ± 1.48 mg/L respectively. The water from the islands of Lake Fitri must therefore be rigorously treated before consumption.
基金funding by the China Postdoctoral Science Foundation(Grant No.:2021M701628)the National Natural Science Foundation of China(Grant No.:82202625)+3 种基金Science and Technology Projects in Guangzhou,China(Grant No.:SL2022A04J02086)President Foundation of Nanfang Hospital,Southern Medical University,China(Grant Nos.:2021B012,and 2021C050)National Science Fund for Distinguished Young Scholars,China(Grant No.:82025024)Key project of the National Natural Science Foundation of China(Grant No.:82230080).
文摘The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.
基金supported by High Level Talents Research Initiation Fund of West Anhui University(WGKQ2022025)Quality Engineering Project of Anhui Province(2024zybj032)+1 种基金Quality Engineering Project of West Anhui University(wxxy2024011)Development of Big Data Integration and Analysis Platform for Traditional Chinese Medicine Genomics(0045025050).
文摘1 Plant SecondaryMetabolism and Functional Biology Progress Plants have long been recognized as biochemical powerhouses,producing a vast array of compounds through their secondary metabolic pathways[1].Although historically referred to as‘secondary’due to their perceived non-essential role in basic plant survival,it is now understood that these metabolites are integral to plant growth,development and adaptation to environmental challenges.Secondary metabolites,such as alkaloids,terpenoids,phenolics and flavonoids,serve as critical mediators of plant-environment interactions,conferring resistance to biotic and abiotic stressors[2].Beyond their ecological functions,these compounds are invaluable to humans,supporting industries ranging from pharmaceuticals and nutrition to agrochemicals and chemical additives.
文摘A spice formulation study in Burkina Faso was carried out using local ingredients for the benefit of households. The objective of this study was to propose some spice formulations based on local ingredients in order to reduce the use of chemical spices in the preparation of different dishes. The Design of Experiments (DOE) methodology was used for the formulation of the spices and their physicochemical, nutritional and sensory characteristics were evaluated by standardized and standard methods. The results obtained showed lipid contents (g/100 g DM) ranging from 10.41 ± 0.26 to 15.64 ± 0.68, total sugars from 4.39 ± 0.32 to 5.46 ± 0.31, protein from 3.65 ± 0.17 to 12.04 ± 0.35 and ash from 5.83 ± 0.01 to 7.02 ± 0.01. The polyphenol content ranged from 9.09 ± 1.60 to 11.33 ± 0.90, and the flavonoid content ranged from 0.65 ± 0.03 to 1.08 ± 0.13. The sensory analysis carried out showed that the spices have generally satisfactory organoleptic characteristics. These results constitute new information in the diet of populations and are an alternative to the chemical spices used in their cooking.
文摘At the beginning of the 20^(th)century,German scientist Richard Semon introduced the term'engram'to describe the neural substrate implicated in the processes of memory formation and retrieval[1].The trace of the corresponding biophysical and biochemical changes in the brain responding to an external stimulus is called an engram,and understanding the physical manifestations of memory formation and recall remains a fundamental yet unresolved question[2].
基金supported by Nanjing Science and Technology Bureau International,Hong Kong,Macao,and Taiwan Science and Technology Cooperation Program(No.202401042)。
文摘Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field faces considerable challenges,including the continuous emergence of potential health threats,as well as novel pathogen strains and viruses.The advent and implementation of advanced technologies,such as culture strategies,genomics mining,and artificial intelligence(AI),are facilitating a paradigm shift in pharmaceutical research,introducing innovative methodologies and perspectives.The development and maturation of these technologies have enhanced the exploration of microbial-derived NPs,thereby advancing pharmaceutical research and development.This review synthesizes recent developments in this context,emphasizing their applications in pharmaceutical discovery and development.Through systematic analysis and synthesis,it provides objective insights into the promising prospects and future direction of this essential field.
文摘The current investigation focuses on intertwined relationships of ecology and aquaculture for the benefit of farmers involved in fish farming practices.The study evaluated glucosinolate reduction in black,brown,and white mustard meals as fish feed ingredients for Indian Major Carps.Fish were fed with 10%mustard meal-supplemented diets in three forms:Raw(R),Anti-nutritional Rich(AR),and Anti-nutritional Lowered(AL),alongside a control group using floating feed.The three-month indoor experiment(September-November 2023)was conducted in FRP tanks with triplicate treatments.Blood analysis revealed compromised health in AR-fed carps,with reduced hemoglobin levels in rohu,catla and mrigal and elevated total leukocyte counts indicating inflammation in all the three carps studied here.Liver function was impaired in AR-fed fish,shown by increased alanine transaminase levels,highest in rohu followed by mrigal and catla.Histopathological examination of AR-fed carps liver tissue revealed necrotic spots,deformed hepatocytes,and significant vacuolation.In contrast,AL-fed fish demonstrated improved health parameters through Complete Blood Count analysis,liver function tests,and histo-pathological observations,suggesting successful reduction of anti-nutritional factors in the processed mustard meals.In near future,replacement of unprocessed seed meal with processed seed meal will lead to economic gains in fish farming.
