Objective: to analyze the accuracy of clinical blood biochemical test samples and the single factor influence in the process of sample analysis. Methods: the blood biochemical test samples from January 2021 to Februar...Objective: to analyze the accuracy of clinical blood biochemical test samples and the single factor influence in the process of sample analysis. Methods: the blood biochemical test samples from January 2021 to February 2022 in our hospital were studied. A total of 2100 samples were screened and sorted out, and the final test results were analyzed, which were influenced by the sample analysis process, and the results were abnormal or inaccurate. Review the single factor in the inspection process, and explore the causes of its influence. Results: among the data results of the final blood biochemical test specimens, there were 64 abnormal errors, accounting for 3.05%. By analyzing the influence of different single factors, it is found that the main single factors are the preservation of blood samples, the ratio of anticoagulants to samples, and the collection method of blood samples. Among them, the most frequent occurrence is the collection of blood samples, with a total of 49 cases, accounting for 2.33%. Conclusion: during the biochemical examination of patients blood samples in hospitals, the staff who need to collect blood have a high standard of working ability and can treat each blood sample seriously, so as to enhance the accuracy and reliability of the results of biochemical examination for doctors disease diagnosis. Different influencing factors have different solutions.展开更多
Objective: to analyze the test accuracy and clinical value of the biochemical test of clinical serum lipids. Methods: a total of 88 patients underwent blood lipid test in the clinical laboratory department of our hosp...Objective: to analyze the test accuracy and clinical value of the biochemical test of clinical serum lipids. Methods: a total of 88 patients underwent blood lipid test in the clinical laboratory department of our hospital from January 2021 to January 2022.Classification test and drag-test are used to compare the accuracy of test results and compare the test cost. Results: the positive rate of the test result is different from the test method, and the data is meaningful (P <0.05);the missing rate is lower than the test-test, the data is meaningful (P <0.05);the test cost is lower than the conventional test, and the data is meaningful (t=23.785, P=0.000).Conclusion: for the clinical biochemical testing of blood lipid, the testing accuracy of the graded test method is higher, which can meet the clinical needs, and can reduce the testing cost, and can be promoted.展开更多
Objective: to explore the accuracy of routine test and biochemical test in the clinical diagnosis of adrenal cortical diseases. Methods: in accordance with the principle of random selection in this hospital in Septemb...Objective: to explore the accuracy of routine test and biochemical test in the clinical diagnosis of adrenal cortical diseases. Methods: in accordance with the principle of random selection in this hospital in September 2019 ~ 2020, 9 months were a total of 70 patients with adrenocortical disease in diagnosis experiment, will be more patients were divided into two groups, control group (n = 35) adopts the method of routine inspection, observation group (n = 35) are taking biochemical testing method, finally compare two groups of patients with the diagnosis accuracy. Results: the test accuracy of observation group was much higher than control group, and P < 0.05. Conclusion: compared with the conventional examination method, the application of biochemical examination technology in adrenal cortical diseases can effectively improve the accuracy of disease diagnosis, provide effective guidance for clinical treatment, has higher application value, and is worthy of popularization and application.展开更多
Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the resul...Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the results were compared. Results: compared with routine test, biochemical test has higher sensitivity, specificity and accuracy with significant difference (P<0.05). Conclusion: biochemical test has more clinical value in the diagnosis of diabetes.展开更多
Objective: this paper mainly analyzes the practical value of biochemical tests in the current diagnosis of liver cirrhosis diseases. Methods: 60 patients were diagnosed in January 2019-2-2020 and were divided into obs...Objective: this paper mainly analyzes the practical value of biochemical tests in the current diagnosis of liver cirrhosis diseases. Methods: 60 patients were diagnosed in January 2019-2-2020 and were divided into observation and control groups. Biochemical tests were performed for all patients, such as total cholesterol, cholinesterase, total bile acid, and serum albumin. Results: Chinesterase and total cholesterol and serum albumin were lower than control and higher total bile acid, with statistically significant P <0.05. Conclusion: the use of biochemical test items in patients with cirrhosis disease can improve diagnostic accuracy and promote promotion.展开更多
Objective: to evaluate the effectiveness of management of diagnosis of liver cirrhosis patients by implementing biochemical test items for liver function in combination with liver cirrhosis patients. Methods: to study...Objective: to evaluate the effectiveness of management of diagnosis of liver cirrhosis patients by implementing biochemical test items for liver function in combination with liver cirrhosis patients. Methods: to study and evaluate the diagnosis results of patients with liver cirrhosis by biochemical experiments. Therefore, 65 patients with liver cirrhosis who came to our hospital from January to December 2020 were selected as the research observers and divided into the research group and the control group, and 65 healthy volunteers who underwent biochemical tests at the same time were taken as the control group. The two groups were compared in terms of physical examination to observe liver cirrhosis, and the specific functional indicators of biochemical test items were applied. Results: combined with the actual situation of patients with liver cirrhosis, clinical biochemical test items were applied to comprehensively improve the scientific basis of clinical treatment application, the civilian test for early diagnosis of related liver cirrhosis diseases, blood glucose test and urine routine test were performed, and the results of various indicators of clinical test were compared between the two groups of patients, so as to comprehensively improve the specific situation of biochemical indicators in the test results of the two groups of patients. The treatment functions of the research group were significantly better than those of the control group, showing that the patients in the research group were significantly higher than those in the control group. The relevant data of all nine biochemical indicators had clinical reliable basis, with statistical difference (P < 0.05). Conclusion: to comprehensively improve the physical examination method of relevant biochemical test items for patients with liver cirrhosis can provide reliable basis for clinical diagnosis and effective guidance for follow-up treatment.展开更多
Objective: to explore the value of liver function biochemical test in the diagnosis of liver cirrhosis patients. Methods: 40 patients with liver cirrhosis who were diagnosed and treated in our department from June 201...Objective: to explore the value of liver function biochemical test in the diagnosis of liver cirrhosis patients. Methods: 40 patients with liver cirrhosis who were diagnosed and treated in our department from June 2019 to June 2020 were randomly selected as the observation group, while the control group was selected from the patients who received physical examination at the same time. Also, 40 patients were randomly selected as the control group. Both groups received biochemical test of liver function. Venous blood was mainly extracted and centrifuged according to the sample requirements of the automatic biochemical analyzer. Serum was added into the automatic biochemical analyzer to detect relevant indicators and the results of the two groups were compared and analyzed. Results: the TBA (45.62±6.21) UMOL/L of the observation group was higher than that of the control group (7.31±0.77), the difference was statistically significant. The ALB (34.83±0.58) g/l, CHE(2634.83±258)U/L) u/l, CHO (3.65±0.36) mmol/l in the observation group were lower than those in the control group (39.65±0.97) g/l, CHE(5844.83±626)U/L) u/l, CHO (4.89±2.73) mmol/l), and the difference was statistically significant (P < 0.05). Conclusion: the application of liver function biochemical test in the diagnosis and treatment of patients with hepatitis and liver cirrhosis is helpful for doctors to reasonably judge the degree of liver tissue damage and has clinical application value.展开更多
Objective: to explore the clinical application of biochemical examination in the diagnosis of diabetes mellitus and to analyze its application value. Methods: 120 patients with biochemical examination in our hospital ...Objective: to explore the clinical application of biochemical examination in the diagnosis of diabetes mellitus and to analyze its application value. Methods: 120 patients with biochemical examination in our hospital from January 2020 to June 2020 were selected, including 60 patients with diabetes as the study group and 60 patients without diabetes as the control group. Biochemical tests were carried out in the two groups, including FBG (fasting blood glucose), HbA1c (glycosylated hemoglobin), 2hPG (2-hour glucose tolerance test) and TG (serum triglyceride). Results: the FBG, HbA1c, 2hPG and TG indices in the study group were significantly higher than those in the control group. Conclusions: biochemical tests can be used to accurately screen and diagnose diabetic patients and to assess their levels of FBG, HbA1c, 2HPG and TG indices. This has practical value.展开更多
Objective: to explore the application effect of blood biochemical test in the diagnosis of diabetes. Methods: patients with diabetes admitted to our hospital in recent years were selected, and the related personnel we...Objective: to explore the application effect of blood biochemical test in the diagnosis of diabetes. Methods: patients with diabetes admitted to our hospital in recent years were selected, and the related personnel were also classified and divided into two groups. Except for the high and low levels of blood glucose, the other conditions of the two groups were basically the same. All patients were tested for blood biochemistry, and the changes of their blood glucose were recorded to observe whether there was any abnormal blood glucose. The results showed that fasting blood glucose, blood glucose at two hours after meal, glycosylated hemoglobin and other indicators in the experimental group were relatively better, and there was a significant difference between the two groups of patients, which had statistical significance and could be used as a guide for subsequent theoretical research. Conclusion: in the diagnosis of diabetes, the use of blood biochemical test can ensure the accuracy of the diagnosis and reflect the relevant indicators of patients, which is worth selecting.展开更多
Objective: the experiment will carry out blood biochemical test for diabetic patients and analyze the clinical effect of the scheme for disease diagnosis. Methods: the experiment selected elderly patients with diabete...Objective: the experiment will carry out blood biochemical test for diabetic patients and analyze the clinical effect of the scheme for disease diagnosis. Methods: the experiment selected elderly patients with diabetes as the main body of the investigation, and the patients admitted from January 2019 to December 2019 were cut off in the time period. The patients were divided into two groups by using the random number method for comparative study, and the changes of the disease conditions of 200 patients were recorded. Patients in the control group were given routine test measures, while patients in the observation group were given blood biochemical test to analyze the differences of test methods. Results: in terms of the quality of examination, the blood biochemical examination of the patients in the observation group was better than that of the control group. Under the blood biochemical examination, the examination specificity, sensitivity and accuracy of the patients in the observation group were 25% (1/4), 98.5% (193/196) and 96.5% (193/200) respectively, which were better than 50% (2/4), 94.4% (185/196) and 92.5% (185/200) of the control group. The comparison difference was more significant and had statistical significance (P < 0.05). Conclusion: the blood biochemical test scheme has higher accuracy, can be used for more accurate analysis of diabetes and has clinical promotion value.展开更多
Objective: to explore the clinical application effect of biochemical test in the diagnosis of diabetes. Methods: this experiment is selected, our hospital admitted 60 cases of diabetes patients, as the object of this ...Objective: to explore the clinical application effect of biochemical test in the diagnosis of diabetes. Methods: this experiment is selected, our hospital admitted 60 cases of diabetes patients, as the object of this experiment. The treatment period was from April 2018 to June 2019. According to the disease of diabetes, they were divided into general diabetes group (experimental group) and observation group (diabetes complications group) for analysis. At the same time, normal patients receiving biochemical tests were selected as the detection objects. Among them, the concentrations of fasting blood glucose (FPG), OGTT(oral glucose tolerance test) and glycosylated hemoglobin (HbAlc) were compared. Results: the FPG, OGTT and HbAlc of the experimental group were significantly higher than those of the observation group and the healthy group (P<0.05), and the difference was statistically significant. Conclusion: in the clinical test, the biochemical test for the diagnosis of diabetes is helpful to distinguish the blood glucose of diabetes, and has the value of promotion.展开更多
Cold atmospheric-pressure plasma is a new technology, widely used in many fields of biomedicine,especially in cancer treatment. Cold plasma can selectively kill a variety of tumor cells, and its biological safety in c...Cold atmospheric-pressure plasma is a new technology, widely used in many fields of biomedicine,especially in cancer treatment. Cold plasma can selectively kill a variety of tumor cells, and its biological safety in clinical trials is also very important. In many cases, the patient’s immune level is relatively low, so we first studied the safety assessment of plasma treatment in an immunocompromised animal model. In this study, we examined the safety of immuno-deficient nude mice by oral lavage treatment of plasma-activated water, and studied the growth status, main organs and blood biochemical indexes. Acute toxicity test results showed that the maximum dose of plasma treatment for 15 min had no lethal effect and other acute toxicity. There were no significant changes in body weight and survival status of mice after 2 min and 4 min of plasma-activated water(PAW)treatment for 2 weeks. After treatment, the major organs, including heart, liver, spleen, lung and kidney, were not significantly changed in organ coefficient and tissue structure. Blood biochemical markers showed that blood neutrophils and mononuclear cells were slightly increased, and the others remained unchanged. Liver function, renal function, electrolytes, glucose metabolism and lipid metabolism were not affected by different doses of PAW treatment. The above results indicate that PAW treatment can be used to treat immuno-deficient nude mice without significant safety problems.展开更多
Water is essential for human life and it’s expected to be clean and safe. In Cameroon, government efforts to improve access to drinking water are mitigated. Many households are suffering from chronic water shortages ...Water is essential for human life and it’s expected to be clean and safe. In Cameroon, government efforts to improve access to drinking water are mitigated. Many households are suffering from chronic water shortages leading to the majority of people using doubtful water sources. This study was carried out in accordance with guidelines produced by the STARBIOS2-EU funded project on Responsible Research and Innovation and aimed to assess the microbiological quality of water samples collected from domestic water sources in 3 divisions of the West region of Cameroon. 22 water samples from well water, stream, water pump and river were aseptically collected. At each sampling point, 50 mL of sample was taken aseptically and immediately transported to the laboratory for analysis. The detection of Coliforms bacteria was done using Micro Biological Survey (MBS) method. 1 mL of each sample was inoculated in the Coliforms MBS (Coli MBS) vial initially rehydrated with 10 mL of sterile distilled water. The Coli MBS vials were closed, shaken for homogenization, and then incubated at 37<span style="white-space:nowrap;">°</span>C. From the initial red color of the Coli MBS vials, changes in color to yellow of the reaction vials were monitored at three different time intervals (12 h, 19 h and 24 h), corresponding to three levels of bacterial concentration. All positives samples on MBS method were selected to determine the presence of <em>E. coli</em> Loop full broths of Coli MBS vials were taken;streaked into the eosin methylene blue (EMB) agar plate and incubated at 37°C for 24 hours. <em>E. coli</em> was confirmed by observing green metallic sheen on EMB agar plate. The biochemical indole, methyl red, Voges-Proskauer and citrate (IMViC) test was performed to determine the complete microbiological profile of the water samples. Almost all the water samples were contaminated with Total Coliforms (TC). A high concentration of TC (>10<sup>3</sup> CFU/mL) was found in 8 samples (36.4%), a medium concentration (10 < x < 10<sup>3</sup> CFU/mL) was observed in 10 samples (45.5%) and 2 samples (9.1%) were low concentrated (1 < x < 10 CFU/mL). 8 samples were positive for the presence of <em>E. coli</em> by observing the green metallic sheen on EMB agar plates. The IMViC test confirmed the presences of 5 bacteria species: <em>Enterobacter spp., Klebsiella spp., E. coli spp., Salmonella spp.</em> and Shigella spp. We recommend integrating sanitary assessment in the design and implementation of water supply projects. Regular water purification using proper methods and maintenances of the water point. Regular microbiological quality assessment of water intended for human consumption should be planned and carried out.展开更多
文摘Objective: to analyze the accuracy of clinical blood biochemical test samples and the single factor influence in the process of sample analysis. Methods: the blood biochemical test samples from January 2021 to February 2022 in our hospital were studied. A total of 2100 samples were screened and sorted out, and the final test results were analyzed, which were influenced by the sample analysis process, and the results were abnormal or inaccurate. Review the single factor in the inspection process, and explore the causes of its influence. Results: among the data results of the final blood biochemical test specimens, there were 64 abnormal errors, accounting for 3.05%. By analyzing the influence of different single factors, it is found that the main single factors are the preservation of blood samples, the ratio of anticoagulants to samples, and the collection method of blood samples. Among them, the most frequent occurrence is the collection of blood samples, with a total of 49 cases, accounting for 2.33%. Conclusion: during the biochemical examination of patients blood samples in hospitals, the staff who need to collect blood have a high standard of working ability and can treat each blood sample seriously, so as to enhance the accuracy and reliability of the results of biochemical examination for doctors disease diagnosis. Different influencing factors have different solutions.
文摘Objective: to analyze the test accuracy and clinical value of the biochemical test of clinical serum lipids. Methods: a total of 88 patients underwent blood lipid test in the clinical laboratory department of our hospital from January 2021 to January 2022.Classification test and drag-test are used to compare the accuracy of test results and compare the test cost. Results: the positive rate of the test result is different from the test method, and the data is meaningful (P <0.05);the missing rate is lower than the test-test, the data is meaningful (P <0.05);the test cost is lower than the conventional test, and the data is meaningful (t=23.785, P=0.000).Conclusion: for the clinical biochemical testing of blood lipid, the testing accuracy of the graded test method is higher, which can meet the clinical needs, and can reduce the testing cost, and can be promoted.
文摘Objective: to explore the accuracy of routine test and biochemical test in the clinical diagnosis of adrenal cortical diseases. Methods: in accordance with the principle of random selection in this hospital in September 2019 ~ 2020, 9 months were a total of 70 patients with adrenocortical disease in diagnosis experiment, will be more patients were divided into two groups, control group (n = 35) adopts the method of routine inspection, observation group (n = 35) are taking biochemical testing method, finally compare two groups of patients with the diagnosis accuracy. Results: the test accuracy of observation group was much higher than control group, and P < 0.05. Conclusion: compared with the conventional examination method, the application of biochemical examination technology in adrenal cortical diseases can effectively improve the accuracy of disease diagnosis, provide effective guidance for clinical treatment, has higher application value, and is worthy of popularization and application.
文摘Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the results were compared. Results: compared with routine test, biochemical test has higher sensitivity, specificity and accuracy with significant difference (P<0.05). Conclusion: biochemical test has more clinical value in the diagnosis of diabetes.
文摘Objective: this paper mainly analyzes the practical value of biochemical tests in the current diagnosis of liver cirrhosis diseases. Methods: 60 patients were diagnosed in January 2019-2-2020 and were divided into observation and control groups. Biochemical tests were performed for all patients, such as total cholesterol, cholinesterase, total bile acid, and serum albumin. Results: Chinesterase and total cholesterol and serum albumin were lower than control and higher total bile acid, with statistically significant P <0.05. Conclusion: the use of biochemical test items in patients with cirrhosis disease can improve diagnostic accuracy and promote promotion.
文摘Objective: to evaluate the effectiveness of management of diagnosis of liver cirrhosis patients by implementing biochemical test items for liver function in combination with liver cirrhosis patients. Methods: to study and evaluate the diagnosis results of patients with liver cirrhosis by biochemical experiments. Therefore, 65 patients with liver cirrhosis who came to our hospital from January to December 2020 were selected as the research observers and divided into the research group and the control group, and 65 healthy volunteers who underwent biochemical tests at the same time were taken as the control group. The two groups were compared in terms of physical examination to observe liver cirrhosis, and the specific functional indicators of biochemical test items were applied. Results: combined with the actual situation of patients with liver cirrhosis, clinical biochemical test items were applied to comprehensively improve the scientific basis of clinical treatment application, the civilian test for early diagnosis of related liver cirrhosis diseases, blood glucose test and urine routine test were performed, and the results of various indicators of clinical test were compared between the two groups of patients, so as to comprehensively improve the specific situation of biochemical indicators in the test results of the two groups of patients. The treatment functions of the research group were significantly better than those of the control group, showing that the patients in the research group were significantly higher than those in the control group. The relevant data of all nine biochemical indicators had clinical reliable basis, with statistical difference (P < 0.05). Conclusion: to comprehensively improve the physical examination method of relevant biochemical test items for patients with liver cirrhosis can provide reliable basis for clinical diagnosis and effective guidance for follow-up treatment.
文摘Objective: to explore the value of liver function biochemical test in the diagnosis of liver cirrhosis patients. Methods: 40 patients with liver cirrhosis who were diagnosed and treated in our department from June 2019 to June 2020 were randomly selected as the observation group, while the control group was selected from the patients who received physical examination at the same time. Also, 40 patients were randomly selected as the control group. Both groups received biochemical test of liver function. Venous blood was mainly extracted and centrifuged according to the sample requirements of the automatic biochemical analyzer. Serum was added into the automatic biochemical analyzer to detect relevant indicators and the results of the two groups were compared and analyzed. Results: the TBA (45.62±6.21) UMOL/L of the observation group was higher than that of the control group (7.31±0.77), the difference was statistically significant. The ALB (34.83±0.58) g/l, CHE(2634.83±258)U/L) u/l, CHO (3.65±0.36) mmol/l in the observation group were lower than those in the control group (39.65±0.97) g/l, CHE(5844.83±626)U/L) u/l, CHO (4.89±2.73) mmol/l), and the difference was statistically significant (P < 0.05). Conclusion: the application of liver function biochemical test in the diagnosis and treatment of patients with hepatitis and liver cirrhosis is helpful for doctors to reasonably judge the degree of liver tissue damage and has clinical application value.
文摘Objective: to explore the clinical application of biochemical examination in the diagnosis of diabetes mellitus and to analyze its application value. Methods: 120 patients with biochemical examination in our hospital from January 2020 to June 2020 were selected, including 60 patients with diabetes as the study group and 60 patients without diabetes as the control group. Biochemical tests were carried out in the two groups, including FBG (fasting blood glucose), HbA1c (glycosylated hemoglobin), 2hPG (2-hour glucose tolerance test) and TG (serum triglyceride). Results: the FBG, HbA1c, 2hPG and TG indices in the study group were significantly higher than those in the control group. Conclusions: biochemical tests can be used to accurately screen and diagnose diabetic patients and to assess their levels of FBG, HbA1c, 2HPG and TG indices. This has practical value.
文摘Objective: to explore the application effect of blood biochemical test in the diagnosis of diabetes. Methods: patients with diabetes admitted to our hospital in recent years were selected, and the related personnel were also classified and divided into two groups. Except for the high and low levels of blood glucose, the other conditions of the two groups were basically the same. All patients were tested for blood biochemistry, and the changes of their blood glucose were recorded to observe whether there was any abnormal blood glucose. The results showed that fasting blood glucose, blood glucose at two hours after meal, glycosylated hemoglobin and other indicators in the experimental group were relatively better, and there was a significant difference between the two groups of patients, which had statistical significance and could be used as a guide for subsequent theoretical research. Conclusion: in the diagnosis of diabetes, the use of blood biochemical test can ensure the accuracy of the diagnosis and reflect the relevant indicators of patients, which is worth selecting.
文摘Objective: the experiment will carry out blood biochemical test for diabetic patients and analyze the clinical effect of the scheme for disease diagnosis. Methods: the experiment selected elderly patients with diabetes as the main body of the investigation, and the patients admitted from January 2019 to December 2019 were cut off in the time period. The patients were divided into two groups by using the random number method for comparative study, and the changes of the disease conditions of 200 patients were recorded. Patients in the control group were given routine test measures, while patients in the observation group were given blood biochemical test to analyze the differences of test methods. Results: in terms of the quality of examination, the blood biochemical examination of the patients in the observation group was better than that of the control group. Under the blood biochemical examination, the examination specificity, sensitivity and accuracy of the patients in the observation group were 25% (1/4), 98.5% (193/196) and 96.5% (193/200) respectively, which were better than 50% (2/4), 94.4% (185/196) and 92.5% (185/200) of the control group. The comparison difference was more significant and had statistical significance (P < 0.05). Conclusion: the blood biochemical test scheme has higher accuracy, can be used for more accurate analysis of diabetes and has clinical promotion value.
文摘Objective: to explore the clinical application effect of biochemical test in the diagnosis of diabetes. Methods: this experiment is selected, our hospital admitted 60 cases of diabetes patients, as the object of this experiment. The treatment period was from April 2018 to June 2019. According to the disease of diabetes, they were divided into general diabetes group (experimental group) and observation group (diabetes complications group) for analysis. At the same time, normal patients receiving biochemical tests were selected as the detection objects. Among them, the concentrations of fasting blood glucose (FPG), OGTT(oral glucose tolerance test) and glycosylated hemoglobin (HbAlc) were compared. Results: the FPG, OGTT and HbAlc of the experimental group were significantly higher than those of the observation group and the healthy group (P<0.05), and the difference was statistically significant. Conclusion: in the clinical test, the biochemical test for the diagnosis of diabetes is helpful to distinguish the blood glucose of diabetes, and has the value of promotion.
基金supported by National Natural Science Foundation of China (Grant No. 51307135)China Postdoctoral Science Foundation (Grant No. 2017M610639)the Fundamental Research Funds for Central Universities
文摘Cold atmospheric-pressure plasma is a new technology, widely used in many fields of biomedicine,especially in cancer treatment. Cold plasma can selectively kill a variety of tumor cells, and its biological safety in clinical trials is also very important. In many cases, the patient’s immune level is relatively low, so we first studied the safety assessment of plasma treatment in an immunocompromised animal model. In this study, we examined the safety of immuno-deficient nude mice by oral lavage treatment of plasma-activated water, and studied the growth status, main organs and blood biochemical indexes. Acute toxicity test results showed that the maximum dose of plasma treatment for 15 min had no lethal effect and other acute toxicity. There were no significant changes in body weight and survival status of mice after 2 min and 4 min of plasma-activated water(PAW)treatment for 2 weeks. After treatment, the major organs, including heart, liver, spleen, lung and kidney, were not significantly changed in organ coefficient and tissue structure. Blood biochemical markers showed that blood neutrophils and mononuclear cells were slightly increased, and the others remained unchanged. Liver function, renal function, electrolytes, glucose metabolism and lipid metabolism were not affected by different doses of PAW treatment. The above results indicate that PAW treatment can be used to treat immuno-deficient nude mice without significant safety problems.
文摘Water is essential for human life and it’s expected to be clean and safe. In Cameroon, government efforts to improve access to drinking water are mitigated. Many households are suffering from chronic water shortages leading to the majority of people using doubtful water sources. This study was carried out in accordance with guidelines produced by the STARBIOS2-EU funded project on Responsible Research and Innovation and aimed to assess the microbiological quality of water samples collected from domestic water sources in 3 divisions of the West region of Cameroon. 22 water samples from well water, stream, water pump and river were aseptically collected. At each sampling point, 50 mL of sample was taken aseptically and immediately transported to the laboratory for analysis. The detection of Coliforms bacteria was done using Micro Biological Survey (MBS) method. 1 mL of each sample was inoculated in the Coliforms MBS (Coli MBS) vial initially rehydrated with 10 mL of sterile distilled water. The Coli MBS vials were closed, shaken for homogenization, and then incubated at 37<span style="white-space:nowrap;">°</span>C. From the initial red color of the Coli MBS vials, changes in color to yellow of the reaction vials were monitored at three different time intervals (12 h, 19 h and 24 h), corresponding to three levels of bacterial concentration. All positives samples on MBS method were selected to determine the presence of <em>E. coli</em> Loop full broths of Coli MBS vials were taken;streaked into the eosin methylene blue (EMB) agar plate and incubated at 37°C for 24 hours. <em>E. coli</em> was confirmed by observing green metallic sheen on EMB agar plate. The biochemical indole, methyl red, Voges-Proskauer and citrate (IMViC) test was performed to determine the complete microbiological profile of the water samples. Almost all the water samples were contaminated with Total Coliforms (TC). A high concentration of TC (>10<sup>3</sup> CFU/mL) was found in 8 samples (36.4%), a medium concentration (10 < x < 10<sup>3</sup> CFU/mL) was observed in 10 samples (45.5%) and 2 samples (9.1%) were low concentrated (1 < x < 10 CFU/mL). 8 samples were positive for the presence of <em>E. coli</em> by observing the green metallic sheen on EMB agar plates. The IMViC test confirmed the presences of 5 bacteria species: <em>Enterobacter spp., Klebsiella spp., E. coli spp., Salmonella spp.</em> and Shigella spp. We recommend integrating sanitary assessment in the design and implementation of water supply projects. Regular water purification using proper methods and maintenances of the water point. Regular microbiological quality assessment of water intended for human consumption should be planned and carried out.
