BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinica...BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinical treatment decisions.Currently,there is no established set of reference intervals for cord blood biochemical parameters of newborns in India,particularly in Mumbai.AIM To create cord blood biochemical parameters reference intervals specifically for Mumbai,India.METHODS A cross-sectional study was conducted in an Indian tertiary care hospital.This study focused on healthy newborns with normal birth weight,born to pregnant mothers without health issues.Cord blood samples,approximately 2-3 mL in volume,were collected from 210 term neonates.These samples were divided into fluoride(glucose)and clot activator(serum)tubes and were subsequently analyzed in the institute's biochemical laboratory.The data obtained from the analysis was then subjected to statistical analysis.The result of the Shapiro-Wilk test suggested non-normality in the data distribution.Consequently,nonparametric statistics were utilized for analysis.The Mann-Whitney U test was utilized to compare parameter distributions among different factors,including the infant’s sex,delivery method,maternal age,and obstetric history.A significance level of P<0.05 was considered to indicate statistical significance.RESULTS The following represent the median figures and central 95 percentile reference intervals for biochemical parameters in umbilical cord blood of newborns:Serum direct bilirubin=(0.1-0.55)mg/dL,indirect bilirubin=(0.64-2.26)mg/dL,total bilirubin=(0.62-3.14)mg/dL,creatinine=(0.27-0.76)mg/dL,sodium=(128.19-143.26)mmol/L,chloride=(100.19-111.68)mmol/L,potassium=(1.62-9.98)mmol/L and plasma glucose=(24.75-94.23)mg/dL.Statistically significant differences were observed in serum sodium,potassium,and plasma glucose levels when comparing delivery modes.CONCLUSION This is the pioneering study in which first time,the biochemical reference intervals in cord blood for newborns are established in western India.The values are applicable for newborns from this area.Larger study throughout the country is required.展开更多
The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due ...The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.展开更多
Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various in...Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various investigations. This cross-sectional study, conducted in Cotonou among 35 azoospermic men, aimed to assess the role of biochemical analysis of seminal fluid in determining the etiological diagnosis. The results revealed significant heterogeneity in biochemical profiles. Most of the patients had normal levels of alpha-glucosidase, fructose, and zinc, indicating normal accessory gland function and suggesting non-obstructive azoospermia. However, a significant subgroup exhibited abnormalities in these markers, suggesting a possible obstructive azoospermia. The heterogeneity in biochemical profiles observed in this study highlights the need for further studies to better characterize azoospermia in Benin.展开更多
Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along th...Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along the southern Mediterranean rim,including Algeria,which primarily focuses on pastoral and forage practices.This study investigates salinity tolerance and ecotypic variability in Vicia narbonensis L.,a fodder legume species recognized for its potential to reclaim marginal soils.Morphological,physiological,and biochemical responses were assessed in three ecotypes(eco2,eco9,and eco10)exposed to different salinity levels(low,moderate,and severe).The study was conducted using a completely randomized block design with three blocks per ecotype per dose.The results from the two-way analysis of variance demonstrate significant effects across nearly all attributes studied,revealing distinct ecotypic responses.These findings underscore variations in growth parameters,osmotic regulation mechanisms,and biochemical adjustments.The substantial diversity observed among these ecotypes in their response to salinity provides valuable insights for breeders addressing both agronomic and ecological challenges.Multivariate analyses,including Principal Component Analysis(PCA),revealed key variables distinguishing between ecotypes under salinity stress.Moreover,Classification based on Salinity Tolerance Indices(STI)further differentiated ecotypic performance with more precision,and this is because of the combination of the different parameters studied.These results open up new prospects for the development of strategies to improve the salinity tolerance of forage legumes.展开更多
A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the...A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the water in Lake Fitri in Chad. Standard microbiology methods were used. Isolated strains of enterobacteria were characterized by API 20E and API Staph galleries and confirmed by API Web. Antibiotic resistance was performed according to the recommendations of the antibiogram committee (CA-SFM, 2019). Physico-chemical analysis of the water was carried out by (Rodier, 2009). The microbiological results revealed an abundance on the Domoro islands (5.37.106 ± 1.5.105 and 1.97.105 ± 4.94.104), successively for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli) and on the Maguite Islands (4.71.106 ± 7.14.105 and 2.32.105 ± 2.86.104), alternately for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli). The results obtained after incubation of the biochemical tests using the API 20E and API Staph galleries and their proposed numerical profile analyzed using Api software and confirmed by Api Web confirmed the contamination of the waters of Lake Fitri by pathogenic strains of E. coli, Staphylococcus and Salmonella spp. The results of the antibiogram carried out show the emergence of certain resistances to Tobramycin, Flucytosine and Teicoplanin. The average levels of BOD5, COD, SS, Nitrate (NO3) and Nitrite (NO2) for Domoro Island were 32.14 ± 2.37 mg/L, 423.86 ± 4.78 mg/L, 65.42 ± 2.27 mg/L, 0.04 ± 0.01 mg/L and 3.32 ± 1.48 mg/L respectively. The water from the islands of Lake Fitri must therefore be rigorously treated before consumption.展开更多
The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point...The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point-of-care testing(POCT)has emerged as a promising alternative,which is awaiting rigorous validation.We assessed 226 samples from patients at the First Affiliated Hospital of Guangzhou Medical University using a Beckman Coulter AU5821 and a PUSHKANG POCT Biochemistry Analyzer MS100.Furthermore,350 samples were evaluated with a Stago coagulation analyzer STAR MAX and a PUSHKANG POCT Coagulation Analyzer MC100.Metrics included thirteen biochemical indexes,such as albumin,and five coagulation indices,such as prothrombin time.Comparisons were drawn against the PUSHKANG POCT analyzer.Bland-Altman plots(MS100:0.8206-0.9995;MC100:0.8318-0.9911)evinced significant consistency between methodologies.Spearman correlation pinpointed a potent linear association between conventional devices and the PUSHKANG POCT analyzer,further underscored by a robust correlation coefficient(MS100:0.713-0.949;MC100:0.593-0.950).The PUSHKANG POCT was validated as a dependable tool for serum and whole blood biochemical and coagulation diagnostics.This emphasizes its prospective clinical efficacy,offering clinicians a swift diagnostic tool and heralding a new era of enhanced patient care outcomes.展开更多
Microbially induced calcite precipitation(MICP)and Enzyme induced calcite precipitation(EICP)techniques were implemented to reinforce the large-scale calcareous sand in this study.Then a coupled numerical model to pre...Microbially induced calcite precipitation(MICP)and Enzyme induced calcite precipitation(EICP)techniques were implemented to reinforce the large-scale calcareous sand in this study.Then a coupled numerical model to predict the biochemical reactions and hydraulic characteristics of MICP and EICP reactions was proposed and verified by physical experiments.Results showed that:This model could describe the variations of bacteria,calcium,calcite,permeability over time reasonably.It is necessary to consider the influence of the calculation domain scale when simulating the convection-diffusionreaction in the multi-process of MICP and EICP reactions.The numerical and experimental values of calcite content are 0.841 g/cm^(3) and 0.861 g/cm^(3) for MICP-reinforced sand,0.263 g/cm^(3) and 0.227 g/cm^(3) for EICP-reinforced sand after 192 h of reaction.The reaction rate k_(rea) is an important parameter to control the calcite content.Accordingly,the permeability coefficient of MICP and EICP reinforced calcareous sand decreases by 32%and 18%.Due to the influence of substance transportation and calcite precipitation,the calcite shows a trend of decreasing firstly and then increasing with the enhancing of the initial permeability coefficient in biochemical reactions.The optimal injecting ratio q11:q12 in this study is 100:300 mL/min.The process for the application of MICP and EICP coupled numerical model is also recommended,which provides reference for engineering projects in ground improvement.展开更多
[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk...[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk（♂） x XiaoWeiHanYang（ ♀ ） were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D （seven lambs） were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D （ P 〉 O. 05 ）, the control were higher significandy than the group B,C （P 〈0.05）. The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control （P〈0.05 ）. Plasma urea nitrogen （PUN） of group B and C were higher than group A significantly （P〈0.05 ） ;Total protein （ TP）, Plasma albumin （ALB） and Plasma total amino acid were no significant difference among the other 3 groups （P〉0.05 ）. [Conclusion] So, the milk-replacers were available for early-weaned lamb.展开更多
Bitter tea is a special kind of tea germplasm in China.The major biochemical components of 24 bitter teas and other 8 Camellia sinensis var.sinensis and 8 C.sinensis var.assamica tea germplasms,which were stored in th...Bitter tea is a special kind of tea germplasm in China.The major biochemical components of 24 bitter teas and other 8 Camellia sinensis var.sinensis and 8 C.sinensis var.assamica tea germplasms,which were stored in the China National Germplasm Hangzhou Tea Repository(CNGHTR),were analyzed and evaluated.The results showed that no significant differences of major biochemical components affecting the tea quality were found between bitter tea and common tea.According to the processing suitability index,bitter tea was suitable for the manufacturing of black tea;while according to evolutionary indices such as the composition and content of catechin,bitter tea was similar to C.sinensis var.assamica belonging to the relatively primitive type in evolution.The results of cluster analysis indicated that bitter tea was clustered with C.sinensis var.assamica,so it could be considered to belong to C.sinensis var.assamica.展开更多
[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experiment...[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.展开更多
Previous studies have shown that water stress can stimulate biosynthesis of secondary metabolites,and physiological and biochemical characteristics of plant can play a key role in its defense responses to water stress...Previous studies have shown that water stress can stimulate biosynthesis of secondary metabolites,and physiological and biochemical characteristics of plant can play a key role in its defense responses to water stress.So the physiological and biochemical characteristics of potted Scutellaria baicalensis Georgi plants were investigated under continuous water stress condition.The results showed that the water content in roots,stems and leaves,together with chlorophyll content of the leaves decreased as the water stress strengthened.Simultaneously,the specific leaf weight increased,and the content of proline and soluble sugar in Scutellaria baicalensis Georgi leaves increased significantly.The changing trends of the baicalin content in the roots,stems and leaves of Scutellaria baicalensis Georgi were different.It increased continuously in roots,while ascended constantly in the stems and leaves during the early days under the water stress,but decreased sharply under the heavy stress.In conclusion,proline and soluble sugar had a close correlation with the drought resistance of Scutellaria baicalensis Georgi.Moderate drought is in favor of synthesizing the secondary metabolites.展开更多
[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napu...[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.展开更多
The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice（eight in each group）after exposure to TiO2 aerosols（1 500 mg/m3）in a sealed chamber.An...The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice（eight in each group）after exposure to TiO2 aerosols（1 500 mg/m3）in a sealed chamber.Another eight mice are exposed to indoor air to be served as a control group.Lung tissue and blood are collected after euthanizing the animals.The results show that lactate dehydrogenase（LDH）activity increases in all experimental groups.Alanine aminotransferase（ALT）activity and blood urea nitrogen（BUN）levels are increased in the group exposed to TiO2 aerosols for 28 d,and creatinine（Cr）levels are increased in 14 d and 28 d samples（P0.05）.No obvious changes are observed in other serum indexes.Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation,and many TiO2 particles are found in the interstitium of pulmonary alveoli.展开更多
[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects...[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.展开更多
By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were ...By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electricfield.The results showed that compared with the control plants,low-intensity-voltage(1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.展开更多
In this study, to understand the variation regulation of physiological and biochemical indexs of White-Tibetan sheep with seasons, the physiological and bio- chemical indexs of White-Tibetan sheep in Hongyuan Areas of...In this study, to understand the variation regulation of physiological and biochemical indexs of White-Tibetan sheep with seasons, the physiological and bio- chemical indexs of White-Tibetan sheep in Hongyuan Areas of Sichuan Province in four seasons were measured. The results showed that the respiratory change range of white-Tibetan sheep in four seasons was from （32.74±21.33） to （49.96±17.62） times/min, and the variation ranges of body temperature and heart rate were from （39.27±1.05） to （39.73±0.31） ℃ and from （88.75±8.32） to （104.63±10.39） times/min, respectively. The indexes of WBC, MCV, MCH and MCHC in autumn and winter were extremely significantly lower than those in spring and summer, while RBC,HCT and RDW-CV in autumn and winter were extremely significantly higher than those in spring and summer, HGB and PLT changed in different seasons with no signifi- cance. The indexes of AST,TP, ALB, GLO and LDH in summer and autumn were relatively higher than those in winter and spring, while the indexes of PCHE, GLU, CHOL and CA in summer and autumn were relatively lower than those in winter and spring, however, the indexes of ALT and ALP were relatively higher in spring and autumn.展开更多
[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by ...[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by tissue culture method. [Result] La and Zn had little effects on seed germination rate, but significantly inhibited the growth of root and seedling, decreased their fresh weight at higher concentration; the inhibition of La proved higher on Chinese cabbage. The contents of chlorophyll and soluble protein increased at lower concentrations of La and Zn, but decreased at higher concentrations. With increasing doses of La and Zn, the activities of CAT and POD rose gradually, while SOD activity decreased at lower doses and increased at higher doses. Both of La and Zn would promote Of. producing and MDA accumulation. [Conclusion] In higher concentration, La was more poisonous than Zn. It is clear that the poisonous mechanism of La is similar to that of Zn. Hence, La is possibly a kind of new pollutant.展开更多
[Objective] This paper aimed to investigate the effects of cadmium（Cd）stress on physiological and biochemical indexes in different maize cultivars and provide theoretical basis for the study on heavy metal ecotoxico...[Objective] This paper aimed to investigate the effects of cadmium（Cd）stress on physiological and biochemical indexes in different maize cultivars and provide theoretical basis for the study on heavy metal ecotoxicology.[Method] The maize cultivars includeing Tienan 2,Yingdan 11 and Zhengdan 958 which were widely planted in Liaoning Province were selected as the experimental materials.And then the effects of different Cd2＋ concentrations on seed germination,physiological and biochemical indexes of young seedlings were investigated.[Result] The lower Cd2＋ concentration showed some the stimulation effect on seed germination of maize,while the higher concentration had obvious inhibitory effect to some extend.The seed germination of all the maize verities was obviously inhibited when the Cd2＋ concentration was more than 5 mg/L.And with the increasing of the Cd2＋ concentration,the seedling growth,exosmic conductivity and chlorophyll content of the maize varieties with different tolerance to Cd2＋ were distinctly changed.[Conclusion] The resistance of Tienan 2 to Cd2＋ was significantly higher than that of the other two varieties.展开更多
The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx（CK） respectivel...The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx（CK） respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme（CAT,POD,SOD） activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.展开更多
[Objective] The ratio of flesh content and biochemical compositions in muscles of Pachyhynobius shangchengensis were researched in this experiment to provide basic referrences for studying nutritive physiological ecol...[Objective] The ratio of flesh content and biochemical compositions in muscles of Pachyhynobius shangchengensis were researched in this experiment to provide basic referrences for studying nutritive physiological ecology of Pachyhynobius shangchengensis. [Method] The biochemical analytic method was used to detect the ratio of flesh content and biochemical compositions in muscle of Pachyhynobius shangchengensis. [Result] The ratio of flesh contents of female and male Pachyhynobius shangchengensis were 54.93% and 55.84% respectively. The contents of protein, total saccharides and fatty acid of female Pachyhynobius shangchengensis were 15.44% ,1.70% and 0.68% respectively,while the same contents of male Pachyhynobius shangchengensis were 15.67%, 1.89% and 0.53% respectively. The total content of 18 kinds of amino acids was 12.72% （the proportion in fresh weight）, among which the content of 8 kinds of essential amino acids was 4.68%. The essential amino acids index（EAAI） was 72.68 and limiting amino acids was valine. There were 11 kinds of fatty acids in muscles of Pachyhynobius shangchengensis, among which the content of unsaturated fatty acids （80.02%） was higher than that of saturated fatty acids（ 19.98% ）. [ Conclusion] Pachyhynobius shangchengensis was a kind of valuable natural resource belonging to the nutritious type of low fat content and high protein content.展开更多
文摘BACKGROUND The reference ranges for biochemical parameters can fluctuate due to factors like altitude,age,gender,and socioeconomic conditions.These values are crucial for interpreting laboratory data and guide clinical treatment decisions.Currently,there is no established set of reference intervals for cord blood biochemical parameters of newborns in India,particularly in Mumbai.AIM To create cord blood biochemical parameters reference intervals specifically for Mumbai,India.METHODS A cross-sectional study was conducted in an Indian tertiary care hospital.This study focused on healthy newborns with normal birth weight,born to pregnant mothers without health issues.Cord blood samples,approximately 2-3 mL in volume,were collected from 210 term neonates.These samples were divided into fluoride(glucose)and clot activator(serum)tubes and were subsequently analyzed in the institute's biochemical laboratory.The data obtained from the analysis was then subjected to statistical analysis.The result of the Shapiro-Wilk test suggested non-normality in the data distribution.Consequently,nonparametric statistics were utilized for analysis.The Mann-Whitney U test was utilized to compare parameter distributions among different factors,including the infant’s sex,delivery method,maternal age,and obstetric history.A significance level of P<0.05 was considered to indicate statistical significance.RESULTS The following represent the median figures and central 95 percentile reference intervals for biochemical parameters in umbilical cord blood of newborns:Serum direct bilirubin=(0.1-0.55)mg/dL,indirect bilirubin=(0.64-2.26)mg/dL,total bilirubin=(0.62-3.14)mg/dL,creatinine=(0.27-0.76)mg/dL,sodium=(128.19-143.26)mmol/L,chloride=(100.19-111.68)mmol/L,potassium=(1.62-9.98)mmol/L and plasma glucose=(24.75-94.23)mg/dL.Statistically significant differences were observed in serum sodium,potassium,and plasma glucose levels when comparing delivery modes.CONCLUSION This is the pioneering study in which first time,the biochemical reference intervals in cord blood for newborns are established in western India.The values are applicable for newborns from this area.Larger study throughout the country is required.
基金funding by the China Postdoctoral Science Foundation(Grant No.:2021M701628)the National Natural Science Foundation of China(Grant No.:82202625)+3 种基金Science and Technology Projects in Guangzhou,China(Grant No.:SL2022A04J02086)President Foundation of Nanfang Hospital,Southern Medical University,China(Grant Nos.:2021B012,and 2021C050)National Science Fund for Distinguished Young Scholars,China(Grant No.:82025024)Key project of the National Natural Science Foundation of China(Grant No.:82230080).
文摘The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.
文摘Azoospermia, defined as a complete absence of spermatozoa in the ejaculate, is a relatively common condition among infertile men. Establishing the etiological diagnosis can be challenging and often requires various investigations. This cross-sectional study, conducted in Cotonou among 35 azoospermic men, aimed to assess the role of biochemical analysis of seminal fluid in determining the etiological diagnosis. The results revealed significant heterogeneity in biochemical profiles. Most of the patients had normal levels of alpha-glucosidase, fructose, and zinc, indicating normal accessory gland function and suggesting non-obstructive azoospermia. However, a significant subgroup exhibited abnormalities in these markers, suggesting a possible obstructive azoospermia. The heterogeneity in biochemical profiles observed in this study highlights the need for further studies to better characterize azoospermia in Benin.
基金Direction Generale de la Recherche Scientifique et du Developpement Technologique(DGRSDT)Algeria,and the Researchers Supporting Project No.(RSP2025R390),King Saud University,Riyadh,Saudi Arabia.
文摘Salinity stress is a major challenge for global agriculture,particularly in arid and semi-arid regions,limiting plant productivity due to water and soil salinity.These conditions particularly affect countries along the southern Mediterranean rim,including Algeria,which primarily focuses on pastoral and forage practices.This study investigates salinity tolerance and ecotypic variability in Vicia narbonensis L.,a fodder legume species recognized for its potential to reclaim marginal soils.Morphological,physiological,and biochemical responses were assessed in three ecotypes(eco2,eco9,and eco10)exposed to different salinity levels(low,moderate,and severe).The study was conducted using a completely randomized block design with three blocks per ecotype per dose.The results from the two-way analysis of variance demonstrate significant effects across nearly all attributes studied,revealing distinct ecotypic responses.These findings underscore variations in growth parameters,osmotic regulation mechanisms,and biochemical adjustments.The substantial diversity observed among these ecotypes in their response to salinity provides valuable insights for breeders addressing both agronomic and ecological challenges.Multivariate analyses,including Principal Component Analysis(PCA),revealed key variables distinguishing between ecotypes under salinity stress.Moreover,Classification based on Salinity Tolerance Indices(STI)further differentiated ecotypic performance with more precision,and this is because of the combination of the different parameters studied.These results open up new prospects for the development of strategies to improve the salinity tolerance of forage legumes.
文摘A total of fifty (50) samples of water from Lake Fitri (Chad), with 25 samples per island (Domoro and Maguite). The aim of this study was to analyze the bacteriological, biochemical and physico-chemical quality of the water in Lake Fitri in Chad. Standard microbiology methods were used. Isolated strains of enterobacteria were characterized by API 20E and API Staph galleries and confirmed by API Web. Antibiotic resistance was performed according to the recommendations of the antibiogram committee (CA-SFM, 2019). Physico-chemical analysis of the water was carried out by (Rodier, 2009). The microbiological results revealed an abundance on the Domoro islands (5.37.106 ± 1.5.105 and 1.97.105 ± 4.94.104), successively for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli) and on the Maguite Islands (4.71.106 ± 7.14.105 and 2.32.105 ± 2.86.104), alternately for total aerobic mesophilic flora (FMAT), and thermotolerant coliform (E. coli). The results obtained after incubation of the biochemical tests using the API 20E and API Staph galleries and their proposed numerical profile analyzed using Api software and confirmed by Api Web confirmed the contamination of the waters of Lake Fitri by pathogenic strains of E. coli, Staphylococcus and Salmonella spp. The results of the antibiogram carried out show the emergence of certain resistances to Tobramycin, Flucytosine and Teicoplanin. The average levels of BOD5, COD, SS, Nitrate (NO3) and Nitrite (NO2) for Domoro Island were 32.14 ± 2.37 mg/L, 423.86 ± 4.78 mg/L, 65.42 ± 2.27 mg/L, 0.04 ± 0.01 mg/L and 3.32 ± 1.48 mg/L respectively. The water from the islands of Lake Fitri must therefore be rigorously treated before consumption.
基金supported by the National Natural Science Foundation of China(No.82302607)the Guangdong Basic and Applied Basic Research Foundation(Nos.2022A1515110555 and 2023A1515010932)+3 种基金the State Key Laboratory of Respiratory Disease(No.SKLRD-Z-202410)the Precision Medicine Joint Foundation of Basic and Applied Basic Research Foundation of Guangdong Province(No.2021B1515230008)the Independent Project of Guangdong-Hong Kong-Macao Joint Laboratory for Respiratory Infectious Diseases(No.GHMJLRID-Z-202102)the Guangdong Zhong Nanshan Medical Foundation(No.ZNSXS-20220015).
文摘The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point-of-care testing(POCT)has emerged as a promising alternative,which is awaiting rigorous validation.We assessed 226 samples from patients at the First Affiliated Hospital of Guangzhou Medical University using a Beckman Coulter AU5821 and a PUSHKANG POCT Biochemistry Analyzer MS100.Furthermore,350 samples were evaluated with a Stago coagulation analyzer STAR MAX and a PUSHKANG POCT Coagulation Analyzer MC100.Metrics included thirteen biochemical indexes,such as albumin,and five coagulation indices,such as prothrombin time.Comparisons were drawn against the PUSHKANG POCT analyzer.Bland-Altman plots(MS100:0.8206-0.9995;MC100:0.8318-0.9911)evinced significant consistency between methodologies.Spearman correlation pinpointed a potent linear association between conventional devices and the PUSHKANG POCT analyzer,further underscored by a robust correlation coefficient(MS100:0.713-0.949;MC100:0.593-0.950).The PUSHKANG POCT was validated as a dependable tool for serum and whole blood biochemical and coagulation diagnostics.This emphasizes its prospective clinical efficacy,offering clinicians a swift diagnostic tool and heralding a new era of enhanced patient care outcomes.
基金supports from the National Key R&D Program of China(Grant No.2023YFB4203301)National Natural Science Foundation of China(Grant No.52238008)Postdoctoral Fellowship Program of CPSF(Grant No.GZC20241516).
文摘Microbially induced calcite precipitation(MICP)and Enzyme induced calcite precipitation(EICP)techniques were implemented to reinforce the large-scale calcareous sand in this study.Then a coupled numerical model to predict the biochemical reactions and hydraulic characteristics of MICP and EICP reactions was proposed and verified by physical experiments.Results showed that:This model could describe the variations of bacteria,calcium,calcite,permeability over time reasonably.It is necessary to consider the influence of the calculation domain scale when simulating the convection-diffusionreaction in the multi-process of MICP and EICP reactions.The numerical and experimental values of calcite content are 0.841 g/cm^(3) and 0.861 g/cm^(3) for MICP-reinforced sand,0.263 g/cm^(3) and 0.227 g/cm^(3) for EICP-reinforced sand after 192 h of reaction.The reaction rate k_(rea) is an important parameter to control the calcite content.Accordingly,the permeability coefficient of MICP and EICP reinforced calcareous sand decreases by 32%and 18%.Due to the influence of substance transportation and calcite precipitation,the calcite shows a trend of decreasing firstly and then increasing with the enhancing of the initial permeability coefficient in biochemical reactions.The optimal injecting ratio q11:q12 in this study is 100:300 mL/min.The process for the application of MICP and EICP coupled numerical model is also recommended,which provides reference for engineering projects in ground improvement.
基金Supported by Span Program of Ministry of Agriculture(KY2001-15)~~
文摘[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk（♂） x XiaoWeiHanYang（ ♀ ） were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D （seven lambs） were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D （ P 〉 O. 05 ）, the control were higher significandy than the group B,C （P 〈0.05）. The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control （P〈0.05 ）. Plasma urea nitrogen （PUN） of group B and C were higher than group A significantly （P〈0.05 ） ;Total protein （ TP）, Plasma albumin （ALB） and Plasma total amino acid were no significant difference among the other 3 groups （P〉0.05 ）. [Conclusion] So, the milk-replacers were available for early-weaned lamb.
基金Supported by the"Study on High Efficiency Machining and Multiple Utilization Technology of Tea Germplasm Resource"of National Science&Technology Supporting Project(2006BAD06B01)"Data Standard of Perennial and Vegetative Propagation Crop Germplasm Resources as a Share Experimental Unit"of National Fundamental Resources Platform of Science&Technology Project(2005DKA21002-08)~~
文摘Bitter tea is a special kind of tea germplasm in China.The major biochemical components of 24 bitter teas and other 8 Camellia sinensis var.sinensis and 8 C.sinensis var.assamica tea germplasms,which were stored in the China National Germplasm Hangzhou Tea Repository(CNGHTR),were analyzed and evaluated.The results showed that no significant differences of major biochemical components affecting the tea quality were found between bitter tea and common tea.According to the processing suitability index,bitter tea was suitable for the manufacturing of black tea;while according to evolutionary indices such as the composition and content of catechin,bitter tea was similar to C.sinensis var.assamica belonging to the relatively primitive type in evolution.The results of cluster analysis indicated that bitter tea was clustered with C.sinensis var.assamica,so it could be considered to belong to C.sinensis var.assamica.
基金Supported by Program for New Century Excellent Talents in University(NCET-04-0907)the Innovative Research Team in University (IRT0453)~~
文摘[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.
基金Supported by Agricultural Seed Project in Shandong Province Research in Screening Varieties of Bulk Authentic Chinese Herbal Medicines(NO.2005LZ08-01)Special Issues of Major Technologyin Shandong Province(NO.2006GGll09078)~~
文摘Previous studies have shown that water stress can stimulate biosynthesis of secondary metabolites,and physiological and biochemical characteristics of plant can play a key role in its defense responses to water stress.So the physiological and biochemical characteristics of potted Scutellaria baicalensis Georgi plants were investigated under continuous water stress condition.The results showed that the water content in roots,stems and leaves,together with chlorophyll content of the leaves decreased as the water stress strengthened.Simultaneously,the specific leaf weight increased,and the content of proline and soluble sugar in Scutellaria baicalensis Georgi leaves increased significantly.The changing trends of the baicalin content in the roots,stems and leaves of Scutellaria baicalensis Georgi were different.It increased continuously in roots,while ascended constantly in the stems and leaves during the early days under the water stress,but decreased sharply under the heavy stress.In conclusion,proline and soluble sugar had a close correlation with the drought resistance of Scutellaria baicalensis Georgi.Moderate drought is in favor of synthesizing the secondary metabolites.
基金Supported by Rapeseed Industry Construction Program of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.
文摘The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice（eight in each group）after exposure to TiO2 aerosols（1 500 mg/m3）in a sealed chamber.Another eight mice are exposed to indoor air to be served as a control group.Lung tissue and blood are collected after euthanizing the animals.The results show that lactate dehydrogenase（LDH）activity increases in all experimental groups.Alanine aminotransferase（ALT）activity and blood urea nitrogen（BUN）levels are increased in the group exposed to TiO2 aerosols for 28 d,and creatinine（Cr）levels are increased in 14 d and 28 d samples（P0.05）.No obvious changes are observed in other serum indexes.Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation,and many TiO2 particles are found in the interstitium of pulmonary alveoli.
基金Supported by Natural Science Foundation of Fujian Province(2008J0052 )Technology Program in Xiamen City(3502Z20072007)~~
文摘[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.
基金Supported by Natural Science Foundation of Shanghai(10ZR1400300 )Central University Special Foundation of Basic Research and Operating expenses+1 种基金Creative Group Foundation of the National Natural Science Foundation of China (50721006)Key Discipline construction Project of Shanghai (B604)~~
文摘By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electricfield.The results showed that compared with the control plants,low-intensity-voltage(1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201003061)~~
文摘In this study, to understand the variation regulation of physiological and biochemical indexs of White-Tibetan sheep with seasons, the physiological and bio- chemical indexs of White-Tibetan sheep in Hongyuan Areas of Sichuan Province in four seasons were measured. The results showed that the respiratory change range of white-Tibetan sheep in four seasons was from （32.74±21.33） to （49.96±17.62） times/min, and the variation ranges of body temperature and heart rate were from （39.27±1.05） to （39.73±0.31） ℃ and from （88.75±8.32） to （104.63±10.39） times/min, respectively. The indexes of WBC, MCV, MCH and MCHC in autumn and winter were extremely significantly lower than those in spring and summer, while RBC,HCT and RDW-CV in autumn and winter were extremely significantly higher than those in spring and summer, HGB and PLT changed in different seasons with no signifi- cance. The indexes of AST,TP, ALB, GLO and LDH in summer and autumn were relatively higher than those in winter and spring, while the indexes of PCHE, GLU, CHOL and CA in summer and autumn were relatively lower than those in winter and spring, however, the indexes of ALT and ALP were relatively higher in spring and autumn.
基金Supported by National Natural Science Foundation of China(No.30900071)Start-up Foundation for Doctor Scientific Research in Shandong University of Technology(4041-406027)~~
文摘[Objective] The aim of this study was to explore the physiological and biochemical responses of Chinese cabbage to La and Zn. [Method] The effects of La and Zn on seed germination and seedling growth were explored by tissue culture method. [Result] La and Zn had little effects on seed germination rate, but significantly inhibited the growth of root and seedling, decreased their fresh weight at higher concentration; the inhibition of La proved higher on Chinese cabbage. The contents of chlorophyll and soluble protein increased at lower concentrations of La and Zn, but decreased at higher concentrations. With increasing doses of La and Zn, the activities of CAT and POD rose gradually, while SOD activity decreased at lower doses and increased at higher doses. Both of La and Zn would promote Of. producing and MDA accumulation. [Conclusion] In higher concentration, La was more poisonous than Zn. It is clear that the poisonous mechanism of La is similar to that of Zn. Hence, La is possibly a kind of new pollutant.
基金Supported by Key National Social and Scientific Fund(09AZD026)Shenyang University Doctor Initial FundKey National Natural Science Fund(40930739)~~
文摘[Objective] This paper aimed to investigate the effects of cadmium（Cd）stress on physiological and biochemical indexes in different maize cultivars and provide theoretical basis for the study on heavy metal ecotoxicology.[Method] The maize cultivars includeing Tienan 2,Yingdan 11 and Zhengdan 958 which were widely planted in Liaoning Province were selected as the experimental materials.And then the effects of different Cd2＋ concentrations on seed germination,physiological and biochemical indexes of young seedlings were investigated.[Result] The lower Cd2＋ concentration showed some the stimulation effect on seed germination of maize,while the higher concentration had obvious inhibitory effect to some extend.The seed germination of all the maize verities was obviously inhibited when the Cd2＋ concentration was more than 5 mg/L.And with the increasing of the Cd2＋ concentration,the seedling growth,exosmic conductivity and chlorophyll content of the maize varieties with different tolerance to Cd2＋ were distinctly changed.[Conclusion] The resistance of Tienan 2 to Cd2＋ was significantly higher than that of the other two varieties.
基金Supported by Science and Technology Innovation Team Project of Ningbo Province of China(2011B82019)Supported by Natural Science Foundation of Ningbo Province of China(Y13C150014)+1 种基金Supported by Foundation of Zhejiang Educational Committee(2011C32008)Supported by Science and Technology Project of Ningbo Province,China(2010C91049)~~
文摘The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx（CK） respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme（CAT,POD,SOD） activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.
基金Supported by the Science and Technology Key Program of Anyang City~~
文摘[Objective] The ratio of flesh content and biochemical compositions in muscles of Pachyhynobius shangchengensis were researched in this experiment to provide basic referrences for studying nutritive physiological ecology of Pachyhynobius shangchengensis. [Method] The biochemical analytic method was used to detect the ratio of flesh content and biochemical compositions in muscle of Pachyhynobius shangchengensis. [Result] The ratio of flesh contents of female and male Pachyhynobius shangchengensis were 54.93% and 55.84% respectively. The contents of protein, total saccharides and fatty acid of female Pachyhynobius shangchengensis were 15.44% ,1.70% and 0.68% respectively,while the same contents of male Pachyhynobius shangchengensis were 15.67%, 1.89% and 0.53% respectively. The total content of 18 kinds of amino acids was 12.72% （the proportion in fresh weight）, among which the content of 8 kinds of essential amino acids was 4.68%. The essential amino acids index（EAAI） was 72.68 and limiting amino acids was valine. There were 11 kinds of fatty acids in muscles of Pachyhynobius shangchengensis, among which the content of unsaturated fatty acids （80.02%） was higher than that of saturated fatty acids（ 19.98% ）. [ Conclusion] Pachyhynobius shangchengensis was a kind of valuable natural resource belonging to the nutritious type of low fat content and high protein content.
