We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpS...We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate （P〈0.01）. These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.展开更多
Skin aging is a complex biological process driven by oxidative stress,chronic inflammation,and the disruption of extracellular matrix homeostasis.Collagen peptides,as bioactive hydrolysates derived from enzymatic hydr...Skin aging is a complex biological process driven by oxidative stress,chronic inflammation,and the disruption of extracellular matrix homeostasis.Collagen peptides,as bioactive hydrolysates derived from enzymatic hydrolysis or microbial fermentation,have demonstrated considerable potential in delaying skin aging.This review sys-tematically summarizes the preparation,isolation,purification,and structural characterization of collagen peptides,along with their anti-aging activity evaluation using both in vitro and in vivo models.Particular emphasis is placed on their multi-target mechanisms,which involve the modulation of key signaling pathways,including NF-κB,MAPK,TGF-β,Nrf2,and PI3K/Akt,to exert antioxidant and anti-inflammatory effects.Despite promising advances,challenges such as low oral bioavailability and the translational gap between experimental models and clinical efficacy persist.Future directions point toward sustainable sourcing,intelligent production technologies,and personalized formulation strategies.This review provides a comprehensive scientific founda-tion to support the continued exploration and application of collagen peptides in anti-skin-aging interventions.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.U1304307)the Young Core Instructor Foundation from the Education Commission of Henan Province,China(Grant No.2014GGJS-056)
文摘We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate （P〈0.01）. These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.
基金supported by Natural Science Fund of Jiangsu Agrianimal Husbandry Vocational College(NSF2022ZR01)Taizhou Science and Technology Support Plan(TSL202524)+1 种基金Zhongke Zhigu International Pharmaceutical Biotechnology(Guangdong)Co.,Ltd.Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Skin aging is a complex biological process driven by oxidative stress,chronic inflammation,and the disruption of extracellular matrix homeostasis.Collagen peptides,as bioactive hydrolysates derived from enzymatic hydrolysis or microbial fermentation,have demonstrated considerable potential in delaying skin aging.This review sys-tematically summarizes the preparation,isolation,purification,and structural characterization of collagen peptides,along with their anti-aging activity evaluation using both in vitro and in vivo models.Particular emphasis is placed on their multi-target mechanisms,which involve the modulation of key signaling pathways,including NF-κB,MAPK,TGF-β,Nrf2,and PI3K/Akt,to exert antioxidant and anti-inflammatory effects.Despite promising advances,challenges such as low oral bioavailability and the translational gap between experimental models and clinical efficacy persist.Future directions point toward sustainable sourcing,intelligent production technologies,and personalized formulation strategies.This review provides a comprehensive scientific founda-tion to support the continued exploration and application of collagen peptides in anti-skin-aging interventions.
