Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity effica...Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.展开更多
Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive ...Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive components derived from jujube fruit have significant nutritional and potential biological effects.In this paper,the latest progress in research on major bioactive compounds obtained from jujube is reviewed,and the potential biological functions of jujube fruit resources are discussed.As a dietary supplement,jujube fruit is well recognized as a healthy food which contains a variety of bioactive substances,such as polysaccharides,polyphenols,amino acids,nucleotides,fatty acids,dietary fiber,alkaloids,and other nutrients.These nutrients and non-nutritive phytochemicals obtained from jujube fruit have physiological functions including anticancer,antioxidant,anti-inflammatory,anti-hyperlipidemic,anti-hyperglycemic,immunoregulatory,neuroprotective,sedative,and antiviral functions.Of note is that new constituents,including alkaloids,dietary fiber,and other bioactive substances,as well as the antiviral,hypoglycemic,lipid-lowering,and neuroprotective effects of jujube fruit,are systematically reviewed here for the first time.Meanwhile,problems affecting the exploitation of jujube fruit resources are discussed and further research directions proposed.Therefore,this review provides a useful bibliography for the future development of jujube-based products and the utilization of jujube nutritional components in functional foods.展开更多
Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug development.Recent studies have shown...Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug development.Recent studies have shown that surface plasmon resonance(SPR)technology is promising in this field.In the present study,we propose an SPR-based integrated strategy to screen and analyze the major active components of TCM.We used Radix Paeoniae Alba(RPA)as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1(TNF-R1).First,RPA extraction was analyzed using an SPR-based screening system,and the potential active ingredients were collected,enriched,and identified as paeoniflorin and paeonol.Next,the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 mM,respectively.Then,SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-a(TNF-a)while binding to the subdomain 1 site of TNF-R1.Finally,in biological assays,the two compounds suppressed cytotoxicity and apoptosis induced by TNF-a in the L929 cell line.These findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug development.The SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization.展开更多
Qingke(highland hull-less barley)is a grain replete with substantial nutrients and bioactive ingredients.In this study,we evaluated the effects of boiling(BO),steaming(ST),microwave baking(MB),far-infrared baking(FB),...Qingke(highland hull-less barley)is a grain replete with substantial nutrients and bioactive ingredients.In this study,we evaluated the effects of boiling(BO),steaming(ST),microwave baking(MB),far-infrared baking(FB),steam explosion(SE),and deep frying(DF)on bioactive components,phenolic compounds,and antioxidant activities of Qingke compared with the effects of traditional roast(TR).Results showed that the soluble dietary fiber,beta-glucan and water-extractable pentosans of Qingke in dry heat processes of TR,SE,MB and FB had a higher content compared with other thermal methods and had a better antioxidant activity of hydroxyl radical scavenging and a better reduction capacity,while those in wet heat processes of BO and ST had a better antioxidant activity of ABTS radical scavenging and a better Fe^(2+) chelating ability.DF-and SE-Qingke had a higher content of tocopherol,phenolic,and flavonoid.Overall,6 free phenolic compounds and 12 bound phenolic compounds of Qingke were identified,and free phenolic compounds suffered more damage during thermal processing.Principal component analysis showed that SE had more advantages in retaining and improving the main biological active ingredients of Qingke,and it may be the best method for treating Qingke.展开更多
Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China.However,the progress of functional peptides minin...Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China.However,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data.In this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences.By functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to longevity.We have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide database.Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is reliable.This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.展开更多
To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field plant...To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.展开更多
Cardiovascular disease is a complex and multifactorial disease strongly associated with hypercholesterolemia, hypertension, reduced ?brinolysis, increased blood-clotting time and increased platelet aggregation. Among ...Cardiovascular disease is a complex and multifactorial disease strongly associated with hypercholesterolemia, hypertension, reduced ?brinolysis, increased blood-clotting time and increased platelet aggregation. Among other confounding factors, oxidative damage is a major factor in cardiovascular and cerebrovascular diseases. The use of garlic in folkloric medicine highlights the potential role in either preventing or delaying cardiovascular disease. Contemporary research reveals how garlic and bioactive components modulate the risk factors of cardiovascular and cerebrovascular diseases thus tending towards validating a measure of folkloric claims concerning the functional efficacy of garlic. This review seeks to elucidate contemporary research attempt towards the validation of the role of garlic and bioactive components, mechanisms of action involved and to define its ultimate role in the prevention and treatment of cardiovascular and cerebrovascular diseases. Some contradictory claims exist possibly due to the use of different experimental protocols/formulations/dosages of garlic. Overwhelming commonality of positive cardiovascular and cerebro-vascular responses reside among research studies thus offering compelling evidence in supports of the bene?cial health effects of garlic and components.展开更多
To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were ...To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS) and high-performance liquid chromatography(HPLC).A total of 75 volatile components were tentatively identified.Laomaner(LME),Laobanzhang(LBZ),and other teas on Bulang Mountain(BL) contained 70,53,and 71 volatile compounds,respectively.Among the volatile compounds,alcohols(30.2%-45.8%),hydrocarbons(13.7%-17.5%),and ketones(12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties.The average content of polyphenol was highest in LME(102.1 mg/g),followed by BL(98.7 mg/g) and LBZ(88.0 mg/g),while caffeine showed the opposite trend,27.3 mg/g in LME,33.5 mg/g in BL,and 38.1 mg/g in LBZ.Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties,while partial least squares-discriminant analysis(PLS-DA) showed satisfactory discrimination.Thirty-four volatile components and five bioactive compounds were selected as major discriminators(variable importance in projection(VIP)>1) among the tea varieties.These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain.展开更多
Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioa...Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was analyzed.Spores contain various bioactive nutrients that are benefi cial to the human body.Mature mushrooms can actively discharge spores in a process affected by light,relative humidity,and temperature.During storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting bodies.The flavor of the fruiting bodies also varied signifi cantly during SD.Edible mushroom sexual spores have the potential to become new raw materials for functional food and medical resources.Research on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.展开更多
Food-derived bioactive components(BC)are celebrated for their health-enhancing properties,but their application was limited in terms of the low solubility,weak stability,and poor bioavailability.To overcome these chal...Food-derived bioactive components(BC)are celebrated for their health-enhancing properties,but their application was limited in terms of the low solubility,weak stability,and poor bioavailability.To overcome these challenges,various nano-delivery systems have been developed and extensively investigated.This review focused on the most prominent nano-delivery systems,such as nanoparticles have emerged as versatile carriers,allowing for improved solubility and controlled release of BC.Nano-emulsions offer enhanced bioavailability and stability,particularly for lipophilic BC.Liposomes,with their cell-membrane-like structure,facilitate efficient intracellular delivery.Solid lipid nanoparticles and nanostructured lipid carriers provide options for controlled release and protection against environmental factors.Additionally,various novel nano-delivery systems have been designed to cater to specific ingredient and application requirements.This review highlighted their advantages,challenges,and recent breakthroughs.The intricate interplay between nano-carrier properties and BC characteristics was elucidated,offering valuable insights for the development of functional foods with enhanced health benefits.展开更多
Ganoderma lucidum is a Chinese medicinal fungus with a long history of use in healthcare and disease treatment.G.lucidum spores(GLS)are tiny germ cells released from the mushroom cap during the mature stage of growth....Ganoderma lucidum is a Chinese medicinal fungus with a long history of use in healthcare and disease treatment.G.lucidum spores(GLS)are tiny germ cells released from the mushroom cap during the mature stage of growth.They contain all the genetic active substances of G.lucidum.G.lucidum spore oil(GLSO)is a lipid component extracted from broken-walled Ganoderma spores using supercritical CO_(2)extraction technology.GLSO contains fatty acids,Ganoderma triterpenes,sterols and other bioactive compounds.Previous studies have demonstrated that GLSO has a wide range of pharmacological properties,including anti-tumor,anti-aging,neuroprotection,immunomodulation,hepatoprotection and modulation of metabolic diseases.This review summarizes the research progress of GLSO over the past two decades in terms of its bioactive components,extraction and processing techniques,pharmacological effects and safety evaluation.This provides a solid foundation for further research and application of GLSO.展开更多
Edible fungi are recognized as the novel superfood due to low fat,high protein and multiple bioactivities.Despite its widespread popularity worldwide,the action mechanisms underlying its health benefits require explor...Edible fungi are recognized as the novel superfood due to low fat,high protein and multiple bioactivities.Despite its widespread popularity worldwide,the action mechanisms underlying its health benefits require exploration to clarify its structure-activity relationship and guide future industry practice.This review overviews the biosynthesis of edible fungus-derived active metabolites(polyphenols,terpenoids,lovastatin,and others),and further elaborates the specific molecular mechanisms of beneficial characteristics(anti-diabetic,anti-inflammatory,and neuroprotective properties).Moreover,the functional performance of edible fungi in various food manufacturing domains(meat,plant protein,fermented beverages,etc.)have been discussed.Significantly,current processing techniques,unpleasant sensory experience,potential safety risks,and incomplete by-product utilization system constrain the prevalence of related commodities.Subsequent research should prioritize the innovation of edible fungus-derived bioactive components in food formulations under the premise of mitigating above adverse factors.This paves the way for creating tailored edible fungus-based products in dietary nutrition supplement and chronic disease management.展开更多
Chili fruits are a potential source of phytochemicals and nutrients for food and reconstituted products.Due to its high nutritional and bioactive components,the current study focused on developing chili instant food p...Chili fruits are a potential source of phytochemicals and nutrients for food and reconstituted products.Due to its high nutritional and bioactive components,the current study focused on developing chili instant food products employing hot-air drying method.The effect of the hot-air drying method on physicochemical properties,microbiological quality,retention of bioactive components,phytochemicals,antioxidant properties,and sensory quality of green and red chilies reconstitution products were investigated.HPLC quantification unveiled that fresh red chili product had retained the highest capsaicin(2703.14μg/g)and dihydrocapsaicin(1518μg/g)content on the 0th day.Further-more,UPLC-MS confirmed the presence of eleven phenolic compounds such as gallic acid,chlorogenic acid,caffeic acid,syringic acid,p-coumaric acid,protocatechuic acid,trans-cinnamic acid,ferulic acid,catechin,rutin,and quercetin.Among all,ferulic acid(382.91μg/g)was the most abundant phenolic compound in fresh green chili products,followed by trans-cinnamic acid(73.19μg/g)in green chili reconstituted and catechin(65.66μg/g)in green and red chili reconstituted products.The chili products retained reasonable amounts of bioactive components and antioxidants during storage without microbial growth.The correlation analysis revealed a significant correlation between capsaicinoids,phenolic compounds,and antioxidant properties,which are linearly related in green chili products.This study offers manufacturers a cost-effective technology for producing high-quality chili-reconstituted products rich in essential nutrients and health benefits.展开更多
Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis...Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.展开更多
Bioactive components are nutrients in rice,and the lower milling processing helps minimize their loss.However,their state during rice storage remains uncertain.This study investigated the changes in the bioactive comp...Bioactive components are nutrients in rice,and the lower milling processing helps minimize their loss.However,their state during rice storage remains uncertain.This study investigated the changes in the bioactive component content during rice storage after different degrees of milling(DOM)and the potential mechanism of component loss through lipid oxidation.After 4 weeks of storage,the average retention index of phenols,γ-aminobutyric acid(γ-GABA),and vitamin B1(Vit B1)across all samples was 65.24,25.68,and 63.74%,respectively.Compared to un-milled(DOM=0%)and over-milled rice(DOM=10 and 12%),low-milled rice(DOM=3,6,and 8%)showed a faster rate of component loss,increased lipid oxidation,and lower pH during storage.The degreasing storage verified that milling-induced lipid oxidation was key in component loss,exhibiting a significant negative correlation(p<0.01).Due to the differences in the sensitivity of components to lipid peroxide radicals,γ-GABA experienced the highest loss during the lipid oxidation process,followed by phenols and Vit B1.Reducing storage temperature and humidity alleviated lipid oxidation in low-milled rice,preventing secondary loss of bioactive components.These results provide new insights supporting moderate milling and the preservation of nutrients in rice.展开更多
This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application informa...This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application information was gathered from relevant reports,books,and classic material medica.Research literature and dissertations on C.axillaris chemical constituents and pharmacological activities,available up to 2023,were sourced from electronic databases.Pharmaceutical components such as flavonoids,phenolic acids,triterpenes,lignans,and organic acids were identified in C.axillaris and categorized based on their relevance to cardiovascular roles.Examining the material basis for C.axillaris efficacy in addressing cardiovascular diseases,an analysis of 27 Chinese patent medicines containing Choerospondiatis Fructus(CF)highlighted Myristicae Semen as the most frequently utilized and strongly associated with CF in prescriptions.C.axillaris exhibited notable pharmacological effects,encompassing anti-myocardial ischemia-reperfusion injury,anti-arrhythmic properties,anti-myocardial fibrosis,and hemorheological effects linked to cardiovascular diseases.This review aspires to provide valuable insights for translating traditional applications into modern pharmaceuticals and offers guidance for clinical applications in cardiovascular interventions.展开更多
The objective of this present study is to evaluate the effect of different drying methods(spray drying,freeze drying and hot-air drying)on physicochemical properties and antioxidant ability of G.lucidum extracts(GLEs)...The objective of this present study is to evaluate the effect of different drying methods(spray drying,freeze drying and hot-air drying)on physicochemical properties and antioxidant ability of G.lucidum extracts(GLEs)using various techniques.Meanwhile,combined with free amino acids(FAA)analysis,taste activity values(TAV)analysis and electronic tongue to investigate the relationship between the drying method and the sensory quality of GLEs.The study demonstrates that spray drying GLEs(SDE)has a high water solubility of 96.04%±0.10%due to its low crystallinity and hollow spherical microstructure.SDE and freeze drying GLEs(FDE)contain high levels of polysaccharides and proteins,which facilitates the formation of polysaccharide-protein chelates,thereby improving antioxidant capacity.However,SDE has higher antioxidant activity when its polysaccharide content is the same.SDE has higher thermal stability to maintain the molecular structure of GLEs.Meanwhile,FDE has a bitter FAAs content up to 65.84±1.12 mg/g and TAV of Histidine is 178.70.Overall,the results suggest that spray drying is an appropriate and preferred drying method to maintain the bioactivity and bioavailability of GLEs.These findings may enhance the understanding of the GLEs flavor property and bioavailability associated with drying methods and provide a theoretical basis for G.lucidum processing.展开更多
The C.oleifera oil processing industry generates large amounts of solid wastes,including C.oleifera shell(COS)and C.oleifera cake(COC).Distinct from generally acknowledged lignocellulosic biomass(corn stover,bamboo,bi...The C.oleifera oil processing industry generates large amounts of solid wastes,including C.oleifera shell(COS)and C.oleifera cake(COC).Distinct from generally acknowledged lignocellulosic biomass(corn stover,bamboo,birch,etc.),Camellia wastes contain diverse bioactive substances in addition to the abundant lignocellulosic components,and thus,the biorefinery utilization of C.oleifera processing byproducts involves complicated processing technologies.This reviewfirst summarizes various technologies for extracting and converting the main components in C.oleifera oil processing byproducts into value-added chemicals and biobased materials,as well as their potential applications.Microwave,ultrasound,and Soxhlet extractions are compared for the extraction of functional bioactive components(tannin,flavonoid,saponin,etc.),while solvothermal conversion and pyrolysis are discussed for the conversion of lignocellulosic components into value-added chemicals.The application areas of these chemicals according to their properties are introduced in detail,including utilizing antioxidant and anti-in-flammatory properties of the bioactive substances for the specific application,as well as drop-in chemicals for the substitution of unrenewable fossil fuel-derived products.In addition to chemical production,biochar fabricated from COS and its applications in thefields of adsorption,supercapacitor,soil remediation and wood composites are comprehensively reviewed and discussed.Finally,based on the compositions and structural characteristics of C.oleifera byproducts,the development of full-component valorization strategies and the expansion of the appli-cationfields are proposed.展开更多
Boiling processing is commonly used in post-harvest handling of White Paeony Root （WPR）, in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a h...Boiling processing is commonly used in post-harvest handling of White Paeony Root （WPR）, in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 rain could both make the WPR bright-colored and improve tile contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling （2-10 min） is recommended for post-harvest handling of WPR.展开更多
Research on marine bioactive peptides has mainly focused on characterization of peptides in hydrolysates prepared with commercial industrial enzymes and the usefulness of such hydrolysates in health and functional foo...Research on marine bioactive peptides has mainly focused on characterization of peptides in hydrolysates prepared with commercial industrial enzymes and the usefulness of such hydrolysates in health and functional foods. However, a relevant question is whether digestion of fish proteins with gastrointestinal proteases per se generates peptides that also can have health promoting properties and can reduce, e.g., diabetes 2, inflammation and hypertension either in relation to gastrointestinal digestion or as alternative to industrial proteases. The aim of the study was to investigate hydrolysates obtained from in vitro sequential digestion of salmon muscle and skin with gastrointestinal proteases including pepsin, pancreatic and pancreatic ＋ mucosal proteases for their ability to scavenge ABTS^＋ radicals and inhibit activity of angiotensin I-converting enzyme （ACE） and dipeptidyl peptidase 4 （DPP-4）. Furthermore, it was the aim to study the inhibitory mechanism and stability towards ACE and DPP-4 activity. Analysis of〈 10 kDa hydrolysates showed that gastrointestinal proteases generated peptides with clear radical scavenging activity and DPP-4 and ACE inhibiting activity as well. Hydrolysates from pepsin digestion exhibited the lowest ECso values for radical scavenging activity and ACE inhibition, whereas ECso increased in hydrolysates after subsequent digestion with pancreatic and mucosal proteases. Interestingly, ECso values for the DPP-4 inhibition were hardly affected by sequential digestion. Inhibition modes for the muscle hydrolysates were both competitive and non-competitive, but prolonged incubation showed that the inhibitory properties were unstable and therefore they were probably digested as competitive substrates by gastrointestinal proteases.展开更多
基金The financial support received from the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022,WDZC20200821141349001)Shenzhen Bay Laboratory Startup Fund(21310041,S234602003)。
文摘Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.
基金the National Natural Science Foundation of China(No.U1703105)the Zhejiang Provincial Key R&D Program of China(No.2019C02074)。
文摘Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive components derived from jujube fruit have significant nutritional and potential biological effects.In this paper,the latest progress in research on major bioactive compounds obtained from jujube is reviewed,and the potential biological functions of jujube fruit resources are discussed.As a dietary supplement,jujube fruit is well recognized as a healthy food which contains a variety of bioactive substances,such as polysaccharides,polyphenols,amino acids,nucleotides,fatty acids,dietary fiber,alkaloids,and other nutrients.These nutrients and non-nutritive phytochemicals obtained from jujube fruit have physiological functions including anticancer,antioxidant,anti-inflammatory,anti-hyperlipidemic,anti-hyperglycemic,immunoregulatory,neuroprotective,sedative,and antiviral functions.Of note is that new constituents,including alkaloids,dietary fiber,and other bioactive substances,as well as the antiviral,hypoglycemic,lipid-lowering,and neuroprotective effects of jujube fruit,are systematically reviewed here for the first time.Meanwhile,problems affecting the exploitation of jujube fruit resources are discussed and further research directions proposed.Therefore,this review provides a useful bibliography for the future development of jujube-based products and the utilization of jujube nutritional components in functional foods.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.:82003711 and 81703526)the Shanghai Sailing Program(Grant No.:19YF1459400).
文摘Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug development.Recent studies have shown that surface plasmon resonance(SPR)technology is promising in this field.In the present study,we propose an SPR-based integrated strategy to screen and analyze the major active components of TCM.We used Radix Paeoniae Alba(RPA)as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1(TNF-R1).First,RPA extraction was analyzed using an SPR-based screening system,and the potential active ingredients were collected,enriched,and identified as paeoniflorin and paeonol.Next,the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 mM,respectively.Then,SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-a(TNF-a)while binding to the subdomain 1 site of TNF-R1.Finally,in biological assays,the two compounds suppressed cytotoxicity and apoptosis induced by TNF-a in the L929 cell line.These findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug development.The SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization.
基金financially supported by the 2018 annual three gorges follow-up research project of the three gorges office of the State Council (YYNY-2017-01)
文摘Qingke(highland hull-less barley)is a grain replete with substantial nutrients and bioactive ingredients.In this study,we evaluated the effects of boiling(BO),steaming(ST),microwave baking(MB),far-infrared baking(FB),steam explosion(SE),and deep frying(DF)on bioactive components,phenolic compounds,and antioxidant activities of Qingke compared with the effects of traditional roast(TR).Results showed that the soluble dietary fiber,beta-glucan and water-extractable pentosans of Qingke in dry heat processes of TR,SE,MB and FB had a higher content compared with other thermal methods and had a better antioxidant activity of hydroxyl radical scavenging and a better reduction capacity,while those in wet heat processes of BO and ST had a better antioxidant activity of ABTS radical scavenging and a better Fe^(2+) chelating ability.DF-and SE-Qingke had a higher content of tocopherol,phenolic,and flavonoid.Overall,6 free phenolic compounds and 12 bound phenolic compounds of Qingke were identified,and free phenolic compounds suffered more damage during thermal processing.Principal component analysis showed that SE had more advantages in retaining and improving the main biological active ingredients of Qingke,and it may be the best method for treating Qingke.
基金funded by Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022)。
文摘Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China.However,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data.In this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences.By functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to longevity.We have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide database.Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is reliable.This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.
文摘To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.
文摘Cardiovascular disease is a complex and multifactorial disease strongly associated with hypercholesterolemia, hypertension, reduced ?brinolysis, increased blood-clotting time and increased platelet aggregation. Among other confounding factors, oxidative damage is a major factor in cardiovascular and cerebrovascular diseases. The use of garlic in folkloric medicine highlights the potential role in either preventing or delaying cardiovascular disease. Contemporary research reveals how garlic and bioactive components modulate the risk factors of cardiovascular and cerebrovascular diseases thus tending towards validating a measure of folkloric claims concerning the functional efficacy of garlic. This review seeks to elucidate contemporary research attempt towards the validation of the role of garlic and bioactive components, mechanisms of action involved and to define its ultimate role in the prevention and treatment of cardiovascular and cerebrovascular diseases. Some contradictory claims exist possibly due to the use of different experimental protocols/formulations/dosages of garlic. Overwhelming commonality of positive cardiovascular and cerebro-vascular responses reside among research studies thus offering compelling evidence in supports of the bene?cial health effects of garlic and components.
文摘To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS) and high-performance liquid chromatography(HPLC).A total of 75 volatile components were tentatively identified.Laomaner(LME),Laobanzhang(LBZ),and other teas on Bulang Mountain(BL) contained 70,53,and 71 volatile compounds,respectively.Among the volatile compounds,alcohols(30.2%-45.8%),hydrocarbons(13.7%-17.5%),and ketones(12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties.The average content of polyphenol was highest in LME(102.1 mg/g),followed by BL(98.7 mg/g) and LBZ(88.0 mg/g),while caffeine showed the opposite trend,27.3 mg/g in LME,33.5 mg/g in BL,and 38.1 mg/g in LBZ.Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties,while partial least squares-discriminant analysis(PLS-DA) showed satisfactory discrimination.Thirty-four volatile components and five bioactive compounds were selected as major discriminators(variable importance in projection(VIP)>1) among the tea varieties.These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain.
基金supported by Liaoning Provincial Department of Educational Annual(2019)Scientific Research Fund Project(LSNZD201903)Shenyang Bureau of Science and Technology Annual(2021)Scientific Research Fund Project(21110319)Shenyang Agricultural University,high-end talent introduction fund project(SYAU20160003).
文摘Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was analyzed.Spores contain various bioactive nutrients that are benefi cial to the human body.Mature mushrooms can actively discharge spores in a process affected by light,relative humidity,and temperature.During storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting bodies.The flavor of the fruiting bodies also varied signifi cantly during SD.Edible mushroom sexual spores have the potential to become new raw materials for functional food and medical resources.Research on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.
基金supported by Hubei Provincial Natural Science Foundation of China(2024AFB964 and 2024AFD079)Hubei Education Department Science and Technology Research Program Young Talents Project(Q20231605)+5 种基金Key Research and Development Program of Hubei Province(2023BBB038)Research Funding of Wuhan Polytechnic University(2023RZ080)National Natural Science Foundation of China(32202234)Hubei Provincial Administration of Traditional Chinese Medicine,Traditional Chinese Medicine Research Fund(ZY2023Q033)Shuguang project of Wuhan Science and Technology Bureau(2023KZ00593)Foundation of State Key Laboratory of Utilization of Woody Oil Resource(Grant No.GZKF202208).
文摘Food-derived bioactive components(BC)are celebrated for their health-enhancing properties,but their application was limited in terms of the low solubility,weak stability,and poor bioavailability.To overcome these challenges,various nano-delivery systems have been developed and extensively investigated.This review focused on the most prominent nano-delivery systems,such as nanoparticles have emerged as versatile carriers,allowing for improved solubility and controlled release of BC.Nano-emulsions offer enhanced bioavailability and stability,particularly for lipophilic BC.Liposomes,with their cell-membrane-like structure,facilitate efficient intracellular delivery.Solid lipid nanoparticles and nanostructured lipid carriers provide options for controlled release and protection against environmental factors.Additionally,various novel nano-delivery systems have been designed to cater to specific ingredient and application requirements.This review highlighted their advantages,challenges,and recent breakthroughs.The intricate interplay between nano-carrier properties and BC characteristics was elucidated,offering valuable insights for the development of functional foods with enhanced health benefits.
基金supported by Pearl River S&T Nova Program of Guangzhou(No.201610010113)the Open Research Fund of NMPA Key Laboratory for Rapid Testing Technology of Drugs,Guangdong Institute for Drug Control(No.KF2022002,KF2022006)Research Project of Chinese Medicine in TCM Bureau of Guangdong Province(No.20231202).
文摘Ganoderma lucidum is a Chinese medicinal fungus with a long history of use in healthcare and disease treatment.G.lucidum spores(GLS)are tiny germ cells released from the mushroom cap during the mature stage of growth.They contain all the genetic active substances of G.lucidum.G.lucidum spore oil(GLSO)is a lipid component extracted from broken-walled Ganoderma spores using supercritical CO_(2)extraction technology.GLSO contains fatty acids,Ganoderma triterpenes,sterols and other bioactive compounds.Previous studies have demonstrated that GLSO has a wide range of pharmacological properties,including anti-tumor,anti-aging,neuroprotection,immunomodulation,hepatoprotection and modulation of metabolic diseases.This review summarizes the research progress of GLSO over the past two decades in terms of its bioactive components,extraction and processing techniques,pharmacological effects and safety evaluation.This provides a solid foundation for further research and application of GLSO.
基金supported by grants from the Key Research and Development Plan of Shaanxi Province,China(2023-ZDLNY-43,2023-YBNY-193).
文摘Edible fungi are recognized as the novel superfood due to low fat,high protein and multiple bioactivities.Despite its widespread popularity worldwide,the action mechanisms underlying its health benefits require exploration to clarify its structure-activity relationship and guide future industry practice.This review overviews the biosynthesis of edible fungus-derived active metabolites(polyphenols,terpenoids,lovastatin,and others),and further elaborates the specific molecular mechanisms of beneficial characteristics(anti-diabetic,anti-inflammatory,and neuroprotective properties).Moreover,the functional performance of edible fungi in various food manufacturing domains(meat,plant protein,fermented beverages,etc.)have been discussed.Significantly,current processing techniques,unpleasant sensory experience,potential safety risks,and incomplete by-product utilization system constrain the prevalence of related commodities.Subsequent research should prioritize the innovation of edible fungus-derived bioactive components in food formulations under the premise of mitigating above adverse factors.This paves the way for creating tailored edible fungus-based products in dietary nutrition supplement and chronic disease management.
基金Department of Biotechnology(DBT),Government of India,New Delhi,funded this study under the Institution-based Research Grant(Grant/Award Number:BT/PR25758/NER/95/1300/2017).
文摘Chili fruits are a potential source of phytochemicals and nutrients for food and reconstituted products.Due to its high nutritional and bioactive components,the current study focused on developing chili instant food products employing hot-air drying method.The effect of the hot-air drying method on physicochemical properties,microbiological quality,retention of bioactive components,phytochemicals,antioxidant properties,and sensory quality of green and red chilies reconstitution products were investigated.HPLC quantification unveiled that fresh red chili product had retained the highest capsaicin(2703.14μg/g)and dihydrocapsaicin(1518μg/g)content on the 0th day.Further-more,UPLC-MS confirmed the presence of eleven phenolic compounds such as gallic acid,chlorogenic acid,caffeic acid,syringic acid,p-coumaric acid,protocatechuic acid,trans-cinnamic acid,ferulic acid,catechin,rutin,and quercetin.Among all,ferulic acid(382.91μg/g)was the most abundant phenolic compound in fresh green chili products,followed by trans-cinnamic acid(73.19μg/g)in green chili reconstituted and catechin(65.66μg/g)in green and red chili reconstituted products.The chili products retained reasonable amounts of bioactive components and antioxidants during storage without microbial growth.The correlation analysis revealed a significant correlation between capsaicinoids,phenolic compounds,and antioxidant properties,which are linearly related in green chili products.This study offers manufacturers a cost-effective technology for producing high-quality chili-reconstituted products rich in essential nutrients and health benefits.
基金funded by the Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2023E002,CI2024E003)National Natural Science Foundation of China(82304663)+1 种基金the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ16-XRZ-072,ZZ17-YQ-025,ZXKT22052,and ZXKT22060)the National Key Research and Development Program(2022YFC3501803).
文摘Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.
基金supported by the Shanghai Agricultural Science and Technology Innovation Program(T2023301,I2023007 and T2023305)Program of Shanghai Academic/Technology Research Leader(23XD1430500).
文摘Bioactive components are nutrients in rice,and the lower milling processing helps minimize their loss.However,their state during rice storage remains uncertain.This study investigated the changes in the bioactive component content during rice storage after different degrees of milling(DOM)and the potential mechanism of component loss through lipid oxidation.After 4 weeks of storage,the average retention index of phenols,γ-aminobutyric acid(γ-GABA),and vitamin B1(Vit B1)across all samples was 65.24,25.68,and 63.74%,respectively.Compared to un-milled(DOM=0%)and over-milled rice(DOM=10 and 12%),low-milled rice(DOM=3,6,and 8%)showed a faster rate of component loss,increased lipid oxidation,and lower pH during storage.The degreasing storage verified that milling-induced lipid oxidation was key in component loss,exhibiting a significant negative correlation(p<0.01).Due to the differences in the sensitivity of components to lipid peroxide radicals,γ-GABA experienced the highest loss during the lipid oxidation process,followed by phenols and Vit B1.Reducing storage temperature and humidity alleviated lipid oxidation in low-milled rice,preventing secondary loss of bioactive components.These results provide new insights supporting moderate milling and the preservation of nutrients in rice.
基金supported by grants from the Macao Science and Technology Development Fund(0043/2021/AGJ)the Science and Technology Major Project of Tibetan Autonomous Region of China(XZ202201ZD0001G06)+2 种基金the Natural Science Foundation of Sichuan Province(2022NSFSC1605)the Special Fund of Natural Science for the Central Universities(ZYN2023099)the Engineering Research Center for Pharmaceuticals and Equipments of Sichuan Province Fund(ERCPEF2201)。
文摘This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application information was gathered from relevant reports,books,and classic material medica.Research literature and dissertations on C.axillaris chemical constituents and pharmacological activities,available up to 2023,were sourced from electronic databases.Pharmaceutical components such as flavonoids,phenolic acids,triterpenes,lignans,and organic acids were identified in C.axillaris and categorized based on their relevance to cardiovascular roles.Examining the material basis for C.axillaris efficacy in addressing cardiovascular diseases,an analysis of 27 Chinese patent medicines containing Choerospondiatis Fructus(CF)highlighted Myristicae Semen as the most frequently utilized and strongly associated with CF in prescriptions.C.axillaris exhibited notable pharmacological effects,encompassing anti-myocardial ischemia-reperfusion injury,anti-arrhythmic properties,anti-myocardial fibrosis,and hemorheological effects linked to cardiovascular diseases.This review aspires to provide valuable insights for translating traditional applications into modern pharmaceuticals and offers guidance for clinical applications in cardiovascular interventions.
基金funded by the National Natural Science Foundation of China,No.32202044Zhejiang Provincial Natural Science Foundation,LY17C200017.
文摘The objective of this present study is to evaluate the effect of different drying methods(spray drying,freeze drying and hot-air drying)on physicochemical properties and antioxidant ability of G.lucidum extracts(GLEs)using various techniques.Meanwhile,combined with free amino acids(FAA)analysis,taste activity values(TAV)analysis and electronic tongue to investigate the relationship between the drying method and the sensory quality of GLEs.The study demonstrates that spray drying GLEs(SDE)has a high water solubility of 96.04%±0.10%due to its low crystallinity and hollow spherical microstructure.SDE and freeze drying GLEs(FDE)contain high levels of polysaccharides and proteins,which facilitates the formation of polysaccharide-protein chelates,thereby improving antioxidant capacity.However,SDE has higher antioxidant activity when its polysaccharide content is the same.SDE has higher thermal stability to maintain the molecular structure of GLEs.Meanwhile,FDE has a bitter FAAs content up to 65.84±1.12 mg/g and TAV of Histidine is 178.70.Overall,the results suggest that spray drying is an appropriate and preferred drying method to maintain the bioactivity and bioavailability of GLEs.These findings may enhance the understanding of the GLEs flavor property and bioavailability associated with drying methods and provide a theoretical basis for G.lucidum processing.
基金The authors acknowledge the financial support from the National Natural Science Foundation of China(Grant No.32201509)Hunan Science and Technology Xiaohe Talent Support Project(2022 TJ-XH 013)+6 种基金Science and Technology Innovation Program of Hunan Province(2022RC1156,2021RC2100)State Key Laboratory of Woody Oil Resource Utilization Common Key Technology Innovation for the Green Transformation of Woody Oil(XLKY202205)State Key Laboratory of Woody Oil Resource Utilization Project(2019XK2002)Key Research and Development Program of the State Forestry and Grassland Administration(GLM[2021]95)Hunan Forestry Outstanding Youth Project(XLK202108-1)Changsha Science and Technology Project(kq2202325,kq2107022)Science and Technology Innovation Leading Talent of Hunan Province(2020RC4026).
文摘The C.oleifera oil processing industry generates large amounts of solid wastes,including C.oleifera shell(COS)and C.oleifera cake(COC).Distinct from generally acknowledged lignocellulosic biomass(corn stover,bamboo,birch,etc.),Camellia wastes contain diverse bioactive substances in addition to the abundant lignocellulosic components,and thus,the biorefinery utilization of C.oleifera processing byproducts involves complicated processing technologies.This reviewfirst summarizes various technologies for extracting and converting the main components in C.oleifera oil processing byproducts into value-added chemicals and biobased materials,as well as their potential applications.Microwave,ultrasound,and Soxhlet extractions are compared for the extraction of functional bioactive components(tannin,flavonoid,saponin,etc.),while solvothermal conversion and pyrolysis are discussed for the conversion of lignocellulosic components into value-added chemicals.The application areas of these chemicals according to their properties are introduced in detail,including utilizing antioxidant and anti-in-flammatory properties of the bioactive substances for the specific application,as well as drop-in chemicals for the substitution of unrenewable fossil fuel-derived products.In addition to chemical production,biochar fabricated from COS and its applications in thefields of adsorption,supercapacitor,soil remediation and wood composites are comprehensively reviewed and discussed.Finally,based on the compositions and structural characteristics of C.oleifera byproducts,the development of full-component valorization strategies and the expansion of the appli-cationfields are proposed.
基金supported by a special fund of China State Administration of Traditional Chinese Medicine(No.201307008-2)National High Technology Research and Development Plan of China(863 Plain)(No.2014AA022204)+1 种基金Jiangsu Province Six Talent Project(YY-007)Jiangsu Branch of China Academy of Chinese Medical Science(No.JSBN1301)
文摘Boiling processing is commonly used in post-harvest handling of White Paeony Root （WPR）, in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 rain could both make the WPR bright-colored and improve tile contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling （2-10 min） is recommended for post-harvest handling of WPR.
文摘Research on marine bioactive peptides has mainly focused on characterization of peptides in hydrolysates prepared with commercial industrial enzymes and the usefulness of such hydrolysates in health and functional foods. However, a relevant question is whether digestion of fish proteins with gastrointestinal proteases per se generates peptides that also can have health promoting properties and can reduce, e.g., diabetes 2, inflammation and hypertension either in relation to gastrointestinal digestion or as alternative to industrial proteases. The aim of the study was to investigate hydrolysates obtained from in vitro sequential digestion of salmon muscle and skin with gastrointestinal proteases including pepsin, pancreatic and pancreatic ＋ mucosal proteases for their ability to scavenge ABTS^＋ radicals and inhibit activity of angiotensin I-converting enzyme （ACE） and dipeptidyl peptidase 4 （DPP-4）. Furthermore, it was the aim to study the inhibitory mechanism and stability towards ACE and DPP-4 activity. Analysis of〈 10 kDa hydrolysates showed that gastrointestinal proteases generated peptides with clear radical scavenging activity and DPP-4 and ACE inhibiting activity as well. Hydrolysates from pepsin digestion exhibited the lowest ECso values for radical scavenging activity and ACE inhibition, whereas ECso increased in hydrolysates after subsequent digestion with pancreatic and mucosal proteases. Interestingly, ECso values for the DPP-4 inhibition were hardly affected by sequential digestion. Inhibition modes for the muscle hydrolysates were both competitive and non-competitive, but prolonged incubation showed that the inhibitory properties were unstable and therefore they were probably digested as competitive substrates by gastrointestinal proteases.
