Despite the promising progress in conductive hydrogels made with pure conducting polymer,great challenges remain in the interface adhesion and robustness in longterm monitoring.To address these challenges,Prof.Seung H...Despite the promising progress in conductive hydrogels made with pure conducting polymer,great challenges remain in the interface adhesion and robustness in longterm monitoring.To address these challenges,Prof.Seung Hwan Ko and Taek-Soo Kim’s team introduced a laserinduced phase separation and adhesion method for fabricating conductive hydrogels consisting of pure poly(3,4-ethylenedioxythiophene):polystyrene sulfonate on polymer substrates.The laser-induced phase separation and adhesion treated conducting polymers can be selectively transformed into conductive hydrogels that exhibit wet conductivities of 101.4 S cm^(−1) with a spatial resolution down to 5μm.Moreover,they maintain impedance and charge-storage capacity even after 1 h of sonication.The micropatterned electrode arrays demonstrate their potential in long-term in vivo signal recordings,highlighting their promising role in the field of bioelectronics.展开更多
Precise design and control of molecular self-assembly as living creatures are exciting ideas in the field of nanotechnology.Characterized with predesigned geometries and accurate spatial addressability,programmable DN...Precise design and control of molecular self-assembly as living creatures are exciting ideas in the field of nanotechnology.Characterized with predesigned geometries and accurate spatial addressability,programmable DNA origami nanostructures have been recognized as optimized tools for assembling multiple functional components.A variety of biomolecules can be attached to the nanoscale drawing boards in a site-specific fashion,thus facilitating the precise construction of DNA origami-based materials for studies on biological interface.In this minireview,we highlight the recent advances in the precise construction of DNA origami-based materials with artificial bio-structures and/or biomimicking functions.The regulation of biological functions by these DNA origami-engineered assemblies at the bio-interface has been summarized and discussed.展开更多
A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target can...A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.展开更多
Stretchable/flexible electronics has attracted great interest and attention due to its potentially broad applications in bio-compatible systems. One class of these ultra-thin electronic systems has found promising and...Stretchable/flexible electronics has attracted great interest and attention due to its potentially broad applications in bio-compatible systems. One class of these ultra-thin electronic systems has found promising and important utilities in bio-integrated monitoring and therapeutic devices. These devices can conform to the surfaces of soft bio-tissues such as the epidermis, the epicardium, and the brain to provide portable healthcare functionalities. Upon contractions of the soft tissues, the electronics undergoes compression and buckles into various modes, depending on the stiffness of the tissue and the strength of the interfacial adhesion. These buckling modes result in different kinds of interfacial delamination and shapes of the deformed electronics, which are very important to the proper functioning of the bio- electronic devices. In this paper, detailed buckling mechanics of these thin-film electronics on elastomeric substrates is studied. The analytical results, validated by experiments, provide a very convenient tool for predicting peak strain in the electronics and the intactness of the interface under various conditions.展开更多
Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared （NIR）- responsive surface for controlling cell adhesion was des...Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared （NIR）- responsive surface for controlling cell adhesion was designed by grafting a thermal responsive polymer （poly（N-isopropylacrylamide）, PNIPAM） onto silicon nanowires （SiNWs） instead of the traditional photosensitive moieties. NIR induced the photothermal effect of the SiNWs, and the local heat induced thermodynamic phase transformation of PNIPAM. With the application of NIR radiation, the surface turned to a hydrophobic state, and restored to the hydrophilic state when NIR was switched off, leading to reversible cell adhesion and release. The switchable wettability of the surface and cell adhesion/release occurred efficiently even after 20 cycles. Proteins were anchored on the surface via hydrophobic interactions using NIR; further connection of a cell-capture agent helped in achieving specific cell capture. This dynamic control of cell adhesion via NIR may provide new clues for designing functional nano-biointerfaces.展开更多
基金supported by the National Natural Science Foundation of China(52475610)Zhejiang Provincial Natural Science Foundation of China(LDQ24E050001).
文摘Despite the promising progress in conductive hydrogels made with pure conducting polymer,great challenges remain in the interface adhesion and robustness in longterm monitoring.To address these challenges,Prof.Seung Hwan Ko and Taek-Soo Kim’s team introduced a laserinduced phase separation and adhesion method for fabricating conductive hydrogels consisting of pure poly(3,4-ethylenedioxythiophene):polystyrene sulfonate on polymer substrates.The laser-induced phase separation and adhesion treated conducting polymers can be selectively transformed into conductive hydrogels that exhibit wet conductivities of 101.4 S cm^(−1) with a spatial resolution down to 5μm.Moreover,they maintain impedance and charge-storage capacity even after 1 h of sonication.The micropatterned electrode arrays demonstrate their potential in long-term in vivo signal recordings,highlighting their promising role in the field of bioelectronics.
基金supported by the National Natural Science Foundation of China(22322702,32071389,22025201,22102038,22107022,21721002,21773044)the National Key R&D Program of China National Basic Research Program of China(2021YFA1200302,2021YFA1201201,2018YFA0208900)+2 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDB36000000)the Youth Innovation Promotion Association,CAS,the CAS Interdisciplinary Innovation Team and the K.C.Wong Education Foundation(GJTD-2018-03)CAS Project for Young Scientists in Basic Research(YSBR-036).
文摘Precise design and control of molecular self-assembly as living creatures are exciting ideas in the field of nanotechnology.Characterized with predesigned geometries and accurate spatial addressability,programmable DNA origami nanostructures have been recognized as optimized tools for assembling multiple functional components.A variety of biomolecules can be attached to the nanoscale drawing boards in a site-specific fashion,thus facilitating the precise construction of DNA origami-based materials for studies on biological interface.In this minireview,we highlight the recent advances in the precise construction of DNA origami-based materials with artificial bio-structures and/or biomimicking functions.The regulation of biological functions by these DNA origami-engineered assemblies at the bio-interface has been summarized and discussed.
基金the support of grants from the National Key R&D Program of China(Nos.2018YFA0108202 and 2017YFA0205300)the National Natural Science Foundation of China(Nos.61571429,61801464,61801465,and 81471748)+1 种基金the STS Project of the Chinese Academy of Sciences(NO.KFJ-STS-SCYD-120)the Science and Technology Commission of Shanghai Municipality(Nos.16410711800 and 14391901900)
文摘A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.
基金partial support of this research by the National Natural Science Foundation of China(Grants 11272260,11172022,11572022,51075327,11302038)
文摘Stretchable/flexible electronics has attracted great interest and attention due to its potentially broad applications in bio-compatible systems. One class of these ultra-thin electronic systems has found promising and important utilities in bio-integrated monitoring and therapeutic devices. These devices can conform to the surfaces of soft bio-tissues such as the epidermis, the epicardium, and the brain to provide portable healthcare functionalities. Upon contractions of the soft tissues, the electronics undergoes compression and buckles into various modes, depending on the stiffness of the tissue and the strength of the interfacial adhesion. These buckling modes result in different kinds of interfacial delamination and shapes of the deformed electronics, which are very important to the proper functioning of the bio- electronic devices. In this paper, detailed buckling mechanics of these thin-film electronics on elastomeric substrates is studied. The analytical results, validated by experiments, provide a very convenient tool for predicting peak strain in the electronics and the intactness of the interface under various conditions.
基金Acknowledgements This research is supported by the National Basic Research Program of China （No. 2012CB933800）, National Natural Science Foundation of China （Nos. 21425314, 21501184, 21434009, 21421061 and 21504098）, the Key Research Program of the Chinese Academy of Sdences （No. KJZD-EW-M01）, the National High-tech R＆D Program of China （863 Program） （No. 2013AA032203）, MOST （No. 2013YQ190467）, the Top-Notch Young Talents Program of China, and Beijing Municipal Science ＆ Technology Commission （No. Z161100000116037）.
文摘Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared （NIR）- responsive surface for controlling cell adhesion was designed by grafting a thermal responsive polymer （poly（N-isopropylacrylamide）, PNIPAM） onto silicon nanowires （SiNWs） instead of the traditional photosensitive moieties. NIR induced the photothermal effect of the SiNWs, and the local heat induced thermodynamic phase transformation of PNIPAM. With the application of NIR radiation, the surface turned to a hydrophobic state, and restored to the hydrophilic state when NIR was switched off, leading to reversible cell adhesion and release. The switchable wettability of the surface and cell adhesion/release occurred efficiently even after 20 cycles. Proteins were anchored on the surface via hydrophobic interactions using NIR; further connection of a cell-capture agent helped in achieving specific cell capture. This dynamic control of cell adhesion via NIR may provide new clues for designing functional nano-biointerfaces.
