Three-dimensional(3D)bio-printing is an emerging tissue engineering technology,and its printing parameters have been upgraded to enable in-depth application in cell-cultured meat.However,excellent printable and edible...Three-dimensional(3D)bio-printing is an emerging tissue engineering technology,and its printing parameters have been upgraded to enable in-depth application in cell-cultured meat.However,excellent printable and edible bio-inks for cell-cultured meat are in urgent need of development.Therefore,a low-cost bio-ink based on albumin and gelatin was developed.At first,suitable printability of the bio-ink was determined by rheology analysis,excellent mechanical stability,and excellent mechanical stability of the printed scaffold was also proved by water absorption and degradation rate.Next,the biocompatibility of the scaffold and its interaction with cells were clarified through cell proliferation culture,cell status research and omics analysis.Notably,AG7 demonstrated better printability and AGS7 provided better conditions for cell attachment,proliferation and migration,S-shaped exponential growth curve further revealed the significant advantages of AGS7 scaffolds in cell culture.More importantly,the tissue culture process of muscle cells was simulated to organoid culture,which elucidated the interaction information between cells and scaffolds.This work has filled the vacancy in the industry and provides a novel strategy for the development of production of cell cultured meat.展开更多
Bioprinting is a revolutionary technology within the field of tissue engineering that enables the precise fabrication of three-dimensional(3D)tissue constructs.It combines the principles of engineering and biology to ...Bioprinting is a revolutionary technology within the field of tissue engineering that enables the precise fabrication of three-dimensional(3D)tissue constructs.It combines the principles of engineering and biology to create structures that closely mimic the complexity of native human tissues,facilitating advancements in regenerative medicine and personalized healthcare.This review paper systematically explores the challenges and design requirements in the fabrication of 3D biomimetic tissue constructs,emphasizing the need for advanced bioprinting strategies.Achieving biomimicry involves creating 3D anatomically relevant structures,biomimetic microenvironments,and vascularization.The focus is on overcoming existing bottlenecks through advancements in both fabrication techniques and bio-inks.Future directions in bioprinting are outlined,including multi-modal bioprinting systems,in-situ bioprinting,and the integration of machine learning into bioprinting processes.The critical role of bio-inks and printing methodologies in influencing cell viability is highlighted,providing insights into strategies for enhancing cellular functionality throughout the bioprinting process.Furthermore,the paper addresses post-fabrication considerations,particularly in accelerating tissue maturation,as a pivotal component for advancing the clinical applicability of bioprinted tissues.By navigating through the challenges,innovations,and prospects of advanced bioprinting strategies,this review highlights the transformative impact on tissue engineering.Pushing the boundaries of technological capabilities,these strategies hold the promise of groundbreaking advancements in regenerative medicine and personalized healthcare.Ultimately,the integration of these advanced techniques into bioprinting processes will pave the way for the development of more highly biomimetic and functional bioprinted tissues.展开更多
The technology of three dimensional(3D) printing,also known as additive manufacturing,is a cuttingedge type of fabrication method that utilizes a computer-aided design platform and employs layer-bylayer stacking to co...The technology of three dimensional(3D) printing,also known as additive manufacturing,is a cuttingedge type of fabrication method that utilizes a computer-aided design platform and employs layer-bylayer stacking to construct objects with exceptional flexibility.Due to its capacity to produce a substantial quantity of products within a short period of time,3D printing has emerged as one of the most significant manufacturing technology.Over the past two decades,remarkable advancements have been made in the application of 3D printing technology in the realm of bone tissue engineering.This review presents an innovative and systematic discussion on the potential application of 3D printing technology in bone tissue engineering,particularly in the treatment of infected bone defects.It comprehensively evaluates the materials utilized in 3D printing,highlights the interplay between cells and bone regeneration,and addresses and resolves challenges associated with current 3D printing technology.These challenges include material selection,fabrication of intricate 3D structures,integration of different cell types,streamlining design processes and material selection procedures,enhancing the clinical translational potential of 3D printing technology,and ultimately exploring future applications of four dimensional(4D) printing technology.The 3D printing technology has demonstrated significant potential in the synthesis of bone substitutes,offering consistent mechanical properties and ease of use.It has found extensive applications in personalized implant customization,prosthetic limb manufacturing,surgical tool production,tissue engineering,biological modeling,and cell diagnostics.Simultaneously,3D bioprinting provides an effective solution to address the issue of organ donor shortage.However,challenges still exist in material selection,management of structural complexity,integration of different cell types,and construction of functionally mature tissues.With advancements in multi-material printing techniques as well as bioprinting and 4D printing technologies emerging on the horizon;3D printing holds immense prospects for revolutionizing the means by which infectious bone defects are repaired.展开更多
The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constru...The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constructs have the potential to alleviate these concerns with reduced autoimmune response, high anatomical accuracy, long-term patency and growth potential. The advent of 3D bioprinting technology has further supplemented the technological toolbox, opening up new biofabrication research opportunities and expanding the therapeutic potential of the field. In this review, we highlight the challenges facing those seeking to create artificial tubular tissue with its associated complex macro- and microscopic architecture. Current biofabrication approaches, including 3D printing techniques, are reviewed and future directions suggested.展开更多
Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure ...Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure remains challenging due to the limitations of currently available hydrogels and existing processing techniques.The present study utilized a bioprinting technique to fabricate hydrogel patches and characterize them in terms of printability,mechanical and biological properties.Cell-laden hydrogel(or bio-ink)was formulated from alginate dialdehyde(ADA)and gelatin(GEL)to improve the printability,degradability as well as bioactivity.Five groups of hydrogel compositions were designed to investigate the influence of the oxidation degree of ADA and hydrogels concentration on the properties of printed scaffolds.ADA-GEL hydrogels have generally shown favorable for living cells(EA.hy926 cells and hybrid human umbilical vein endothelial cell line).The hydrogel with an oxidation degree of 10%and a concentration ratio of 70/30(or 10%ADA70-GEL30)demonstrated the best printability among the groups examined.Formulated hydrogels were also bioprinted with the living cells(EA.hy926),and the scaffolds printed were then subject to the cell culture for 7 days.Our results illustrate that the scaffolds bioprinted from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability.Taken together,in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fist time,used them to bioprint cardiac patches,which have the potential to be used in MI repair.展开更多
Tunneling wounds create passageways underneath the skin surface with varying sizes and shapes and can have twists and turns,making their treatment extremely difficult.Available wound care solutions only cater to super...Tunneling wounds create passageways underneath the skin surface with varying sizes and shapes and can have twists and turns,making their treatment extremely difficult.Available wound care solutions only cater to superficial wounds,and untreated tunneling wounds pose major health concerns.This study aims to fulfill this challenge by fabricating tunnel wound fillers(TWFs)made of natural polymers that mimic the dermal extracellular matrix.In this study,cellulose microfibers(CMFs)derived from banana stem and fish skin-derived collagen were used to formulate bio-inks with varying CMF contents(25,50,and 75 mg).Tri-layered(CMFs,primary and secondary collagen coatings),drug-eluting(Baneocin),and cell-laden(human mesenchymal stem cells)TWFs were three-dimensional(3D)-printed and extensively characterized.CMFs showed the most suitable rheological properties for 3D printing at 50 mg concentration.The Alamar Blue data showed significantly increased cell proliferation from Day 1 to Day 7,and scratch tests used to evaluate in vitro wound healing revealed that the best coverage of the wound area was achieved using CMFs in combination with collagen and alginate.Finally,the TWF showed promising capability and tunability in terms of wound shape and size upon testing on a chicken tissue model.The results demonstrate the tremendous potential of TWFs in treating deep tunneling wounds with unique advantages,such as patient-specific customization,good wound exudate absorption capability while releasing wound healing drugs,and the inclusion of stem cells for accelerated healing and tissue regeneration.展开更多
Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evi...Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.展开更多
基金funded under the National key research and development plan(2021YFC2101400)Chinese Academy of Engineering Strategic Research and Consulting Project(2023-XZ-79,2022-30-19)National Natural Science Foundation of China(22005019)。
文摘Three-dimensional(3D)bio-printing is an emerging tissue engineering technology,and its printing parameters have been upgraded to enable in-depth application in cell-cultured meat.However,excellent printable and edible bio-inks for cell-cultured meat are in urgent need of development.Therefore,a low-cost bio-ink based on albumin and gelatin was developed.At first,suitable printability of the bio-ink was determined by rheology analysis,excellent mechanical stability,and excellent mechanical stability of the printed scaffold was also proved by water absorption and degradation rate.Next,the biocompatibility of the scaffold and its interaction with cells were clarified through cell proliferation culture,cell status research and omics analysis.Notably,AG7 demonstrated better printability and AGS7 provided better conditions for cell attachment,proliferation and migration,S-shaped exponential growth curve further revealed the significant advantages of AGS7 scaffolds in cell culture.More importantly,the tissue culture process of muscle cells was simulated to organoid culture,which elucidated the interaction information between cells and scaffolds.This work has filled the vacancy in the industry and provides a novel strategy for the development of production of cell cultured meat.
基金support from NTU Presidential Postdoctoral Fellowshipthe support from the National Research Foundation,Singapore,under its NRF Investigatorship(NRFNRFI07-2021-007,Funding Awardee:Wai Yee Yeong)。
文摘Bioprinting is a revolutionary technology within the field of tissue engineering that enables the precise fabrication of three-dimensional(3D)tissue constructs.It combines the principles of engineering and biology to create structures that closely mimic the complexity of native human tissues,facilitating advancements in regenerative medicine and personalized healthcare.This review paper systematically explores the challenges and design requirements in the fabrication of 3D biomimetic tissue constructs,emphasizing the need for advanced bioprinting strategies.Achieving biomimicry involves creating 3D anatomically relevant structures,biomimetic microenvironments,and vascularization.The focus is on overcoming existing bottlenecks through advancements in both fabrication techniques and bio-inks.Future directions in bioprinting are outlined,including multi-modal bioprinting systems,in-situ bioprinting,and the integration of machine learning into bioprinting processes.The critical role of bio-inks and printing methodologies in influencing cell viability is highlighted,providing insights into strategies for enhancing cellular functionality throughout the bioprinting process.Furthermore,the paper addresses post-fabrication considerations,particularly in accelerating tissue maturation,as a pivotal component for advancing the clinical applicability of bioprinted tissues.By navigating through the challenges,innovations,and prospects of advanced bioprinting strategies,this review highlights the transformative impact on tissue engineering.Pushing the boundaries of technological capabilities,these strategies hold the promise of groundbreaking advancements in regenerative medicine and personalized healthcare.Ultimately,the integration of these advanced techniques into bioprinting processes will pave the way for the development of more highly biomimetic and functional bioprinted tissues.
基金supported by the National Natural Science Fund of China(Nos.82202726,82370929)the National Clinical Research Center for Geriatrics,West China Hospital,Sichuan University(No.Z20192013)+5 种基金Key research and development project of Sichuan Science and Technology Department(No.2023YFG0219)"Zeroto One" Innovation Research Project of Sichuan University(No.2022SCUH0014)Frontiers Medical Center,Tianfu Jincheng Laboratory Foundation(No.TFJC2023010001)Sichuan Science and Technology Program(No.2022NSFSC0002)Sichuan Province Youth Science and Technology Innovation Team(No.2022JDTD0021)Research and Develop Program,West China Hospital of Stomatology Sichuan University(Nos.RD03202302,RCDWJS2024-1)。
文摘The technology of three dimensional(3D) printing,also known as additive manufacturing,is a cuttingedge type of fabrication method that utilizes a computer-aided design platform and employs layer-bylayer stacking to construct objects with exceptional flexibility.Due to its capacity to produce a substantial quantity of products within a short period of time,3D printing has emerged as one of the most significant manufacturing technology.Over the past two decades,remarkable advancements have been made in the application of 3D printing technology in the realm of bone tissue engineering.This review presents an innovative and systematic discussion on the potential application of 3D printing technology in bone tissue engineering,particularly in the treatment of infected bone defects.It comprehensively evaluates the materials utilized in 3D printing,highlights the interplay between cells and bone regeneration,and addresses and resolves challenges associated with current 3D printing technology.These challenges include material selection,fabrication of intricate 3D structures,integration of different cell types,streamlining design processes and material selection procedures,enhancing the clinical translational potential of 3D printing technology,and ultimately exploring future applications of four dimensional(4D) printing technology.The 3D printing technology has demonstrated significant potential in the synthesis of bone substitutes,offering consistent mechanical properties and ease of use.It has found extensive applications in personalized implant customization,prosthetic limb manufacturing,surgical tool production,tissue engineering,biological modeling,and cell diagnostics.Simultaneously,3D bioprinting provides an effective solution to address the issue of organ donor shortage.However,challenges still exist in material selection,management of structural complexity,integration of different cell types,and construction of functionally mature tissues.With advancements in multi-material printing techniques as well as bioprinting and 4D printing technologies emerging on the horizon;3D printing holds immense prospects for revolutionizing the means by which infectious bone defects are repaired.
基金We acknowledge the funding support from UK Engineering and Physical Sciences Research Council （EPSRC） on the Doctoral Prize Fellowship （Grant No. EP/N509760/1） for IH and the EngD studentship （Grant No. EP/L015595/1） for JL. JZS is funded by Overseas Scholarship Council and Ministry of Education in China. We also acknowledge the funding support from China-UK Research and Innovation Partnership Fund： Newton Fund Ph.D. placement programme. We thank the National Natural Science Foundation of China （No. 21534007）, and the Beijing Municipal Science ＆ Technology Commission for their financial support.
文摘The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constructs have the potential to alleviate these concerns with reduced autoimmune response, high anatomical accuracy, long-term patency and growth potential. The advent of 3D bioprinting technology has further supplemented the technological toolbox, opening up new biofabrication research opportunities and expanding the therapeutic potential of the field. In this review, we highlight the challenges facing those seeking to create artificial tubular tissue with its associated complex macro- and microscopic architecture. Current biofabrication approaches, including 3D printing techniques, are reviewed and future directions suggested.
文摘Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure remains challenging due to the limitations of currently available hydrogels and existing processing techniques.The present study utilized a bioprinting technique to fabricate hydrogel patches and characterize them in terms of printability,mechanical and biological properties.Cell-laden hydrogel(or bio-ink)was formulated from alginate dialdehyde(ADA)and gelatin(GEL)to improve the printability,degradability as well as bioactivity.Five groups of hydrogel compositions were designed to investigate the influence of the oxidation degree of ADA and hydrogels concentration on the properties of printed scaffolds.ADA-GEL hydrogels have generally shown favorable for living cells(EA.hy926 cells and hybrid human umbilical vein endothelial cell line).The hydrogel with an oxidation degree of 10%and a concentration ratio of 70/30(or 10%ADA70-GEL30)demonstrated the best printability among the groups examined.Formulated hydrogels were also bioprinted with the living cells(EA.hy926),and the scaffolds printed were then subject to the cell culture for 7 days.Our results illustrate that the scaffolds bioprinted from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability.Taken together,in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fist time,used them to bioprint cardiac patches,which have the potential to be used in MI repair.
基金supported by the start-up funds from New York University Abu Dhabipartially carried out using the Core Technology Platforms resources at New York University Abu Dhabi。
文摘Tunneling wounds create passageways underneath the skin surface with varying sizes and shapes and can have twists and turns,making their treatment extremely difficult.Available wound care solutions only cater to superficial wounds,and untreated tunneling wounds pose major health concerns.This study aims to fulfill this challenge by fabricating tunnel wound fillers(TWFs)made of natural polymers that mimic the dermal extracellular matrix.In this study,cellulose microfibers(CMFs)derived from banana stem and fish skin-derived collagen were used to formulate bio-inks with varying CMF contents(25,50,and 75 mg).Tri-layered(CMFs,primary and secondary collagen coatings),drug-eluting(Baneocin),and cell-laden(human mesenchymal stem cells)TWFs were three-dimensional(3D)-printed and extensively characterized.CMFs showed the most suitable rheological properties for 3D printing at 50 mg concentration.The Alamar Blue data showed significantly increased cell proliferation from Day 1 to Day 7,and scratch tests used to evaluate in vitro wound healing revealed that the best coverage of the wound area was achieved using CMFs in combination with collagen and alginate.Finally,the TWF showed promising capability and tunability in terms of wound shape and size upon testing on a chicken tissue model.The results demonstrate the tremendous potential of TWFs in treating deep tunneling wounds with unique advantages,such as patient-specific customization,good wound exudate absorption capability while releasing wound healing drugs,and the inclusion of stem cells for accelerated healing and tissue regeneration.
基金supported by a grant from the National Research Foundation of Korea funded by the Ministry of education,Science,and Technology(MEST)(Grant NRF-2018R1A2B2005263)supported by the National Research Foundation of Korea(NRF)Grant funded by the Ministry of Science and ICT for Bioinspired Innovation Technology Development Project(NRF-2018M3C1B7021997)supported by a grant from the Ministry of Trade,Industry&Energy(MOTIE,Korea)under Industrial Technology Innovation Program(20009652:Technology on commercialization and materials of Bioabsorbable Hydroxyapatite that is less than micrometer in size).
文摘Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.
