A novel fluorescent composition was firstly isolated from natural winter fresh Moso bamboo shoots,and its optical properties were fully investigated by fluorescence spectroscopy.It could emit strong blue light both in...A novel fluorescent composition was firstly isolated from natural winter fresh Moso bamboo shoots,and its optical properties were fully investigated by fluorescence spectroscopy.It could emit strong blue light both in solid and solution state,providing high fluorescence intensity in ethanol.The solution’s concentration and addition of water greatly affected the fluorescence intensity,high concentration and addition of much water could quench fluorescence.Apoptosis results showed that the fluorescent extract(0-25 mg/L)could not induce apoptosis of Hela cells.Confocal fluorescent microscopic imaging in human hepatocellular carcinoma cells(HepG2)was realized using the fluorescent extract,it could dye the whole cell well which was different from 4',6-diamidino-2-phenylindole(DAPI)only dying cell nucleus.The fluorescent extract may be candidate for future natural fluorescent bio-imaging agent.展开更多
Hydrogen sulfide(H_(2) S) is a signaling molecule that plays important roles in biological systems.The exploration of H_(2) S as a new drug release trigger and its related fluorescent theranostic system is crucial for...Hydrogen sulfide(H_(2) S) is a signaling molecule that plays important roles in biological systems.The exploration of H_(2) S as a new drug release trigger and its related fluorescent theranostic system is crucial for cancer bio-imaging and therapy.Herein,we designed a new two-photon ratiometric fluorescent theranostic prodrug(compound 1) and studied its spectroscopic properties and application in in vivo imaging.Compound 1 specifically reacted with H_(2) S and released the free active therapeutic component of 7-ethyl-10-hydroxycamptothecin,which was accompanied with a red-shift fluorescence emission signal from 460 nm to 545 nm.The exogenous and endogenous H_(2) S in living cells were imaged by compound 1 under one-photon and two-photon excitation.Furthermore,compound 1 monitored the H_(2) S concentration changes in Caenorhabditis elegans by fluorescence imaging.Additionally,it showed effective drug release activation in situ tumor with exogenous and endogenous H_(2) S as the trigger.The H_(2) S-sensitive activation and drug-release properties highlight the potential of theranostic compound 1 in future cancer treatment and therapy.展开更多
The rapidly growing field of functional,molecular and structural bio-imaging is providing an extraordinary new opportunity to overcome the limits of invasive liver biopsy and introduce a"digital biopsy"for i...The rapidly growing field of functional,molecular and structural bio-imaging is providing an extraordinary new opportunity to overcome the limits of invasive liver biopsy and introduce a"digital biopsy"for in vivo study of liver pathophysiology.To foster the application of bio-imaging in clinical and translational research,there is a need to standardize the methods of both acquisition and the storage of the bio-images of the liver.It can be hoped that the combination of digital,liquid and histologic liver biopsies will provide an innovative synergistic tri-dimensional approach to identifying new aetiologies,diagnostic and prognostic biomarkers and therapeutic targets for the optimization of personalized therapy of liver diseases and liver cancer.A group of experts of different disciplines(Special Interest Group for Personalized Hepatology of the Italian Association for the Study of the Liver,Institute for Biostructures and Bio-imaging of the National Research Council and Bio-banking and Biomolecular Resources Research Infrastructure)discussed criteria,methods and guidelines for facilitating the requisite application of data collection.This manuscript provides a multi-Author review of the issue with special focus on fatty liver.展开更多
It is well known that zinc ions play an indispensable role in the structure and function of a large number of biological process and relevant bio-macromolecules. When some cancers occurred, the relevant concentration ...It is well known that zinc ions play an indispensable role in the structure and function of a large number of biological process and relevant bio-macromolecules. When some cancers occurred, the relevant concentration of zinc ions considerably decreased. Since cancer cells have a completely different redox homeostasis from normal cells, in this contribution, we have explored the possibility of bio-imaging or labeling of cancer cells through the in situ biosynthesized zinc nanoclusters by cancerous cells. The results demonstrate that we can readily realize the in vivo fluorescent bio-imaging of cancer cells through the in situ biosynthesis of the biocompatible zinc nanoclusters from cancerous cells （i.e., Hela cervical carcinoma cell line and others） when target cells cultured with micromolar zinc gluconate solutions.展开更多
PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mec...PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mechanisms are still not fully understood.We employed an optical method to elucidate PBQ action via a novel fluorescent viscosity probe,NCV.As the viscosity in the test solutions increased,compared with that in pure ethanol,a 54-fold fluorescence intensity enhancement of NCV was observed in 310 cP of 90%glycerol.Furthermore,NCV successfully exhibited a selective fluorescence response towards monensin-induced cellular viscosity changes in HepG2 cells.The liver,stomach,and foot plantar of the tested snails were frozen and sectioned for fluorescent imaging experiments after the treatment with different PBQ concentrations over various times.A significant fluorescent increase in the snail's liver was observed upon exposure to 0.75 mg/L PBQ for 72 h,which highlighted an increase in viscosity.Hematoxylin and eosin(HE)staining further supported PBQ-induced liver damage with a viscosity increase in P.canaliculata.Our study provides a new rapid optical visualization method to study the killing mechanisms of PBQ and may help discover new chemicals that control snail populations.展开更多
Photo-stimulated luminescence(PSL) is the process in which trapped charges are released by photons and produce luminescence through recombination. The variegated optical characteristics of photostimulated phosphors(PS...Photo-stimulated luminescence(PSL) is the process in which trapped charges are released by photons and produce luminescence through recombination. The variegated optical characteristics of photostimulated phosphors(PSPs) have drawn increasing attention and a large body of work encompassing mechanism and application of PSPs has been addressed. The optical data storage capacity resulting from abundant defect states enables PSPs to be applied to information storage. Moreover, PSPs provide potential application for anti-counterfeiting, as color changes due to the tunneling process. Recently, near infrared(NIR) light PSPs have been developed, exhibiting enormous potential for in vivo bio-imaging, as the stable and high noise-signal ratio characteristic of PSL. In this review, we devote to introducing the development and process of PSPs, and the challenge and future advance have also been demonstrated.展开更多
Nanomaterials with multiple functions have become more and more popular in the domain of cancer research. MoS2 has a great potential in photothermal therapy, X-ray/CT imaging and drug delivery. In this study, a water ...Nanomaterials with multiple functions have become more and more popular in the domain of cancer research. MoS2 has a great potential in photothermal therapy, X-ray/CT imaging and drug delivery. In this study, a water soluble MoS2 nanosystem(MoS2-PEG) was synthesized and explored in drug delivery, photothermal therapy(PTT) and X-ray imaging.Doxorubicin(DOX) was loaded onto MoS2-PEG with a high drug loading efficiency(~69%)and obtained a multifunctional drug delivery system(MoS2-PEG/DOX). As the drug delivery, MoS2-PEG/DOX could efficiently cross the cell membranes, and escape from the endosome via NIR light irradiation, lead to more apoptosis in MCF-7 cells, and afford higher antitumor efficacy without obvious toxic effects to normal organs owing to its prolonged blood circulation and 11.6-fold higher DTX uptake of tumor than DOX. Besides, MoS2-PEG/DOX not only served as a drug delivery system, but also as a powerful PTT agent for thermal ablation of tumor and a strong X-ray contrast agent for tumor diagnosis. In the in vitro and in vivo studies, MoS2-PEG/DOX exhibited excellent tumor-targeting efficacy, outstanding synergistic anti-cancer effect of photothermal and chemotherapy and X-ray imaging property,demonstrating that MoS2-PEG/DOX had a great potential for simultaneous diagnosis and photothermal-chemotherapy in cancer treatment.展开更多
Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the...Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes： （1） patterned illumination- based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; （2） single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluo- rophore at subdiffraction precision; （3） bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.展开更多
Nonlinear optics,which is a subject for studying the interaction between intense light and materials,has great impact on various research fields.Since many structures in biological tissues exhibit strong nonlinear opt...Nonlinear optics,which is a subject for studying the interaction between intense light and materials,has great impact on various research fields.Since many structures in biological tissues exhibit strong nonlinear optical effects,nonlinear optics has been widely applied in biomedical studies.Especially in the aspect of bio-imaging,nonlinear optical techniques can provide rapid,label-free and chemically specific imaging of biological samples,which enable the investigation of biological processes and analysis of samples beyond other microscopy techniques.In this review,we focus on the introduction of nonlinear optical processes and their applications in bio-imaging as well as the recent advances in this filed.Our perspective of this field is also presented.展开更多
Fluorescent organic nanoparticles(NPs)based on aggregation-induced emission(AIE)-active molecules,are widely applied as non-invasive tools in bio-imaging and demonstrate great potential for studying physiological and ...Fluorescent organic nanoparticles(NPs)based on aggregation-induced emission(AIE)-active molecules,are widely applied as non-invasive tools in bio-imaging and demonstrate great potential for studying physiological and pathological processes.In this paper,we report the synthesis of highly emissive AIEgen-based NPs(SCA NPs)via reprecipitation without any carriers as long-term cell trackers.Uniformly sized SCA NPs enjoy the advantages of high brightness,good stability,large Stokes shift,good biocompatibility,and high photostability.The SCA NPs were successfully applied for in vitro bio-imaging of HeLa cells with an excellent cancer cell uptake.In addition,a strong fluorescence from SCA NPs can still be clearly observed in HeLa cells after incubation for six generations over 15 days.Thus,the SCA NPs could be ideal fluorescent probes for non-invasive long-term cellular imaging.The AIE-active NPs display superior performance and provide a basis for the development of fluorescent organic probes for monitoring biological processes.展开更多
Natural resources are practically infinitely abundant in nature,which stimulates scientists to create new materials with inventive uses and minimal environmental impact.Due to the various benefits of natural carbon do...Natural resources are practically infinitely abundant in nature,which stimulates scientists to create new materials with inventive uses and minimal environmental impact.Due to the various benefits of natural carbon dots(NCDs)from them has received a lot of attention recently.Natural products-derived carbon dots have recently emerged as a highly promising class of nanomaterials,showcasing exceptional properties and eco-friendly nature,which make them appealing for diverse applications in various fields such as biomedical,environmental sensing and monitoring,energy storage and conversion,optoelectronics and photonics,agriculture,quantum computing,nanomedicine and cancer therapy.Characterization techniques such as Photoinduced electron transfer,Aggregation-Induced-Emission(AIE),Absorbance,Fluorescence in UV-Vis and NIR Regions play crucial roles in understanding the structural and optical properties of Carbon dots(CDs).The exceptional photoluminescence properties exhibited by CDs derived from natural products have paved the way for applications in tissue engineering,cancer treatment,bioimaging,sensing,drug delivery,photocatalysis,and promising remarkable advancements in these fields.In this review,we summarized the various synthesis methods,physical and optical properties,applications,challenges,future prospects of natural products-derived carbon dots etc.In this expanding sector,the difficulties and prospects for NCD-based materials research will also be explored.展开更多
Novel CdTe/CdS quantum dots(QDs)coated with a hybrid of SiO_2 and ZnS were fabricated through a simple two-step approach.The hybrid SiO_2/ZnS coated CdTe/CdS quantum dots was characterized by transmission electron mic...Novel CdTe/CdS quantum dots(QDs)coated with a hybrid of SiO_2 and ZnS were fabricated through a simple two-step approach.The hybrid SiO_2/ZnS coated CdTe/CdS quantum dots was characterized by transmission electron microscopy(TEM),UV and fluorescence spectrometer.Results indicated that the core-shell structure gave the QDs outstanding photoluminescence properties,includinganarrowphotoluminescencespectrum,high photoluminescence(PL)quantum yield and long emission lifetime(average PL lifetime of increased from 26.4 ns to 49.1 ns).Cellular studies showed the QDs had good cytocompatibility with Hela cells as determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)assay after coating SiO_2/ZnS,and also proved the feasibility of using the hybrid SiO_2/ZnS coated QDs as optical probes for in vitro cell imaging.The synthesis method of QDs is highly promising for the production of robust and functional optical probes for bio-imaging and sensing applications.展开更多
Graphene is the thinest nanomaterial known in the world,which has unique electronic mobility,super specific surface area,high mechanical strength,excellent corrosion resistance and surface chemical structure.Due to it...Graphene is the thinest nanomaterial known in the world,which has unique electronic mobility,super specific surface area,high mechanical strength,excellent corrosion resistance and surface chemical structure.Due to its special nanostructure and excellent physical and chemical properties,graphene has a broad application prospect in the fields of electronics,optics,magnetism,biomedicine,catalysis,energy storage and sensors.In order to better develop and utilize graphene data,this paper reviewed the structural characteristics of graphene,as well as its research progress in biosensors,bio-imaging,aerogel and other biomedical fields,hoping to provide scientific basis for better development of graphene and the development of graphene pharmaceutical products.展开更多
Background:Fluorescence bio-imaging in the second near-infrared window(NIR-II FL,1000-1700nm)has great potential in clinical theranostics,which is of great importance providing precise locations of lesions and molecul...Background:Fluorescence bio-imaging in the second near-infrared window(NIR-II FL,1000-1700nm)has great potential in clinical theranostics,which is of great importance providing precise locations of lesions and molecular dynamic actions simultaneously in a single nanoprobe.Methods:T here has been an upsurge of multidisciplinary research focusing on developing functional types of inorganic and organic nanoprobes that can be used for NIR-II FL with the high spatiotemporal resolution,deep tissue penetration,and negligible auto-fluorescence.Results:In this mini-review,we summarize recent progress in inorganic/organic NIR-II FL nanoprobes.We introduce the design and properties of inorganic and organic nanoprobes,in the order of single-walled carbon nanotubes,quantum dots,rare-earth-doped nanoparticles,metal nanoclusters and organic fluorophores,expect to realize precise diagnosis and efficient image-guided therapy.Conclusion:Meanwhile,to elucidate the problems and perspectives,we aim to offer diverse biological applications of inorganic/organic NIR-II FL nanoprobes and accelerate the clinical transformation progress.展开更多
Poly-substituted olefins,one of the most important aggregation-induced emission luminogens(AIEgens),have garnered significant attention due to their various applications in chemical-and bio-sensing,bio-imaging,and opt...Poly-substituted olefins,one of the most important aggregation-induced emission luminogens(AIEgens),have garnered significant attention due to their various applications in chemical-and bio-sensing,bio-imaging,and opto-electronics.However,the synthetic methods for these olefins remain limited,impeding the progress of AIEgens.This study introduces an unprecedented cross-coupling reaction between aryl sulfonium triflates and tosylhydrazones from naturally abundant thioethers and ketones.The generality of this method is exemplified by the facile synthesis of over forty poly-substituted olefins.Importantly,the luminescent properties of these AIEgens(e.g.,quantum yield and emission color)can be easily tuned by adjusting the substituents of the electrophile and nucleophile substrates,exhibiting excellent performance in bio-imaging.Notably,the mechanistic studies reveal the critical role ofβ-H elimination in the formation of the double bond.This contribution provides an efficient method to synthesize poly-substituted olefins,pushing forward the development of AIEgens.展开更多
Luminescent nanoparticles(LNPs)have emerged as a promising approach for enhanced cell labelling and disease diagnosis by leveraging their unique photophysical and surface characteristics.Advanced generations of LNPs,s...Luminescent nanoparticles(LNPs)have emerged as a promising approach for enhanced cell labelling and disease diagnosis by leveraging their unique photophysical and surface characteristics.Advanced generations of LNPs,such as quantum dots,dye-loaded nanoparticles and up-converting nanoparticles,exhibit distinct properties and advantages tailored for specialised applications.Consequently,there is a growing focus and demand to develop organelle-specific LNPs to identify,treat and elucidate disease mechanisms.The endoplasmic reticulum(ER)represents one such organelle,playing crucial roles in protein synthesis and modification,calcium homeostasis,lipid trafficking,and regulation of cellular stress.The unfolded protein response,regulated by ER stress,is a clinically significant pathway within the ER,implicated in cellular dysfunction and disease.The growing understanding of ER stress and the unfolded protein response has led to a rapid emergence of endoplasmic reticulum-targeting LNPs(ERLNPs)for precise intracellular diagnosis and therapy.This review discusses current advances and design principles of ERLNPs,highlights current achievements and applications,and discusses the challenges and interdisciplinarity needed for future development.展开更多
We constructed a reaction-based near-infrared fluorescent probe(Niap)to specifically identify alkaline phosphatase(ALP)with fast red fluorescence enhancement.Based on the positive concentrationdependent manner between...We constructed a reaction-based near-infrared fluorescent probe(Niap)to specifically identify alkaline phosphatase(ALP)with fast red fluorescence enhancement.Based on the positive concentrationdependent manner between the fluorescent intensity of the Niap and ALP,probe Niap was used to study the ALP enrichment and variation in golden apple snails(Pomacea canaliculata)exposed to the molluscicide candidate PPU06.After treatment with different concentrations of PPU06 over various times,three organs of the surviving snails,liver,stomach and plantaris,were frozen and sectioned for fluorescent imaging experiments.With increased PPU06 concentration,red fluorescence substantially increased in the liver and reached a maximum within 24 h when the PPU06 co ncentration was 0.75 mg/L.No obvious changes in the stomach or foot plantaris were found.It showed PPU06 caused liver injury and stimulated the increase of ALP in the liver of P.canaliculata.This study demonstrates a rapid ALP fluorescent identification method that can be used to study the effects of PPU06 on P.canaliculata.It also provides optical evidence that may aid in the discovery of new chemistry for snail control.展开更多
Luminescent lanthanide complexes have been widely investigated as light emitting materials in bio-imaging and sensing, solid state lighting and display, anti-fake tags and light conversion films, due to their characte...Luminescent lanthanide complexes have been widely investigated as light emitting materials in bio-imaging and sensing, solid state lighting and display, anti-fake tags and light conversion films, due to their characterized photophysical properties including large Stokes shift, long lifetime, and sharp emission spectrum, arising from the sensitized f-f transitions. In this review, we summarize the most recent advances in luminescent lanthanide complexes and their applications from 2015 to August 2017 concerning of general concepts to potential applications. We first introduce the basic concept of sensitized luminescence of lanthanide complexes and the strategies used for highly luminescent complexes. Then recent varieties of luminescent lanthanide complexes and their hybrid materials are presented. Finally, applications are discussed in detail.展开更多
Luminescent metal nanoclusters (NCs) have recently emerged as a novel class of luminescent nanomaterials and hold significant potential in biomedicine owing to their ultrasmall (<2nm) size,excellent photostability,...Luminescent metal nanoclusters (NCs) have recently emerged as a novel class of luminescent nanomaterials and hold significant potential in biomedicine owing to their ultrasmall (<2nm) size,excellent photostability,and good biocompatibility.The recent rapid advances in the synthesis and functionalization of luminescent metal NCs have enabled scientists to develop colorful nanomaterials and nanodevices for a wide range of biomedical applications.In this review,we summarize the characteristics and advantages of luminescence from metal NCs,and highlight their applications in biomedicine.We focus on the research in biomedical detection,bio-imaging,drug delivery,and therapy,especially for the advances in the last five years.Luminescent metal NCs display a series of unique superiorities in biomedical applications,and the recent achievements have brought a lot of benefits to the diagnosis and treatment of clinical diseases,especially for tumors and cancers.Finally,we put forward the main challenges that currently still hinder the basic science studies and the practical development of luminescent metal NCs in biomedical applications.Overall,we expect that luminescent metal NCs will play a much more important role in future biomedicine and clinical applications.展开更多
We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-v...We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-vis)spectroscopy,photoluminescence(PL)spectroscopy,X-ray diffraction(XRD),and transmission electron microscopy(TEM)confirm that the CdTe QDs are formed via an extracellular growth and subsequent endocytosis pathway and have size-tunable optical properties with fluorescence emission from 490 to 560 nm and a cubic zinc blende structure with good crystallinity.In particular,the CdTe QDs with uniform size(2-3.6 nm)are protein-capped,which makes them highly soluble in water,and in situ bio-imaging in yeast cells indicates that the biosynthesized QDs have good biocompatibility.This work provides an economic and environmentally friendly approach to synthesize highly fluorescent biocompatible CdTe QDs for bio-imaging and bio-labeling applications.展开更多
基金by the Science and Technology Planned Projects of Zhejiang Province(2018F10009)the National Key Research&Development(R&D)plan(2016YFD060090305)+2 种基金the Promotion of Project of Forestry Science and Technology of the Chinese Forestry Administration([2016]22)the International Joint Project Between Mars Incorporated and China National Bamboo Research Center(PRSD-026-13)the Science and Technology Planned Projects of Zhejiang Province(2017E8002).
文摘A novel fluorescent composition was firstly isolated from natural winter fresh Moso bamboo shoots,and its optical properties were fully investigated by fluorescence spectroscopy.It could emit strong blue light both in solid and solution state,providing high fluorescence intensity in ethanol.The solution’s concentration and addition of water greatly affected the fluorescence intensity,high concentration and addition of much water could quench fluorescence.Apoptosis results showed that the fluorescent extract(0-25 mg/L)could not induce apoptosis of Hela cells.Confocal fluorescent microscopic imaging in human hepatocellular carcinoma cells(HepG2)was realized using the fluorescent extract,it could dye the whole cell well which was different from 4',6-diamidino-2-phenylindole(DAPI)only dying cell nucleus.The fluorescent extract may be candidate for future natural fluorescent bio-imaging agent.
基金the support of the National Natural Science Foundation of China (Nos.21867019,22067021,22067019)the “Youth Talent of Wan Ren Project” Foundation of Yunnan Province of Chinathe “Lian Da Scholar Project” of Yunnan Normal University。
文摘Hydrogen sulfide(H_(2) S) is a signaling molecule that plays important roles in biological systems.The exploration of H_(2) S as a new drug release trigger and its related fluorescent theranostic system is crucial for cancer bio-imaging and therapy.Herein,we designed a new two-photon ratiometric fluorescent theranostic prodrug(compound 1) and studied its spectroscopic properties and application in in vivo imaging.Compound 1 specifically reacted with H_(2) S and released the free active therapeutic component of 7-ethyl-10-hydroxycamptothecin,which was accompanied with a red-shift fluorescence emission signal from 460 nm to 545 nm.The exogenous and endogenous H_(2) S in living cells were imaged by compound 1 under one-photon and two-photon excitation.Furthermore,compound 1 monitored the H_(2) S concentration changes in Caenorhabditis elegans by fluorescence imaging.Additionally,it showed effective drug release activation in situ tumor with exogenous and endogenous H_(2) S as the trigger.The H_(2) S-sensitive activation and drug-release properties highlight the potential of theranostic compound 1 in future cancer treatment and therapy.
基金Supported by the Italian Ministry of Health Project,No.RF-2010-2314264
文摘The rapidly growing field of functional,molecular and structural bio-imaging is providing an extraordinary new opportunity to overcome the limits of invasive liver biopsy and introduce a"digital biopsy"for in vivo study of liver pathophysiology.To foster the application of bio-imaging in clinical and translational research,there is a need to standardize the methods of both acquisition and the storage of the bio-images of the liver.It can be hoped that the combination of digital,liquid and histologic liver biopsies will provide an innovative synergistic tri-dimensional approach to identifying new aetiologies,diagnostic and prognostic biomarkers and therapeutic targets for the optimization of personalized therapy of liver diseases and liver cancer.A group of experts of different disciplines(Special Interest Group for Personalized Hepatology of the Italian Association for the Study of the Liver,Institute for Biostructures and Bio-imaging of the National Research Council and Bio-banking and Biomolecular Resources Research Infrastructure)discussed criteria,methods and guidelines for facilitating the requisite application of data collection.This manuscript provides a multi-Author review of the issue with special focus on fatty liver.
基金supported by the National Natural Science Foundation of China(No.81325011)National High Technology Research&Development Program of China(No.2015AA020502,2012AA022703)the Major Science&Technology Project of Suzhou(No.ZXY2012028)
文摘It is well known that zinc ions play an indispensable role in the structure and function of a large number of biological process and relevant bio-macromolecules. When some cancers occurred, the relevant concentration of zinc ions considerably decreased. Since cancer cells have a completely different redox homeostasis from normal cells, in this contribution, we have explored the possibility of bio-imaging or labeling of cancer cells through the in situ biosynthesized zinc nanoclusters by cancerous cells. The results demonstrate that we can readily realize the in vivo fluorescent bio-imaging of cancer cells through the in situ biosynthesis of the biocompatible zinc nanoclusters from cancerous cells （i.e., Hela cervical carcinoma cell line and others） when target cells cultured with micromolar zinc gluconate solutions.
基金the financial support of this work by the National Natural Science Foundation of China(Nos.82072309,22067019 and 22367023)the Major Science and Technology Projects in Yunnan Province(No.202402AE090006)+3 种基金the Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform(No.2022YKZY001)Yunnan Provincial Science and Technology Department Yunnan University Joint Special Project(No.202201BF070001-001)the Postgraduate Research Innovation Foundation of Yunnan University(No.KC-23234403)the Scientific Research Foundation Project of Yunnan Provincial Department of Education(No.2023Y0240)。
文摘PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(P.canaliculata)control activity and low toxicity for other aquatic organisms,but its snail-killing mechanisms are still not fully understood.We employed an optical method to elucidate PBQ action via a novel fluorescent viscosity probe,NCV.As the viscosity in the test solutions increased,compared with that in pure ethanol,a 54-fold fluorescence intensity enhancement of NCV was observed in 310 cP of 90%glycerol.Furthermore,NCV successfully exhibited a selective fluorescence response towards monensin-induced cellular viscosity changes in HepG2 cells.The liver,stomach,and foot plantar of the tested snails were frozen and sectioned for fluorescent imaging experiments after the treatment with different PBQ concentrations over various times.A significant fluorescent increase in the snail's liver was observed upon exposure to 0.75 mg/L PBQ for 72 h,which highlighted an increase in viscosity.Hematoxylin and eosin(HE)staining further supported PBQ-induced liver damage with a viscosity increase in P.canaliculata.Our study provides a new rapid optical visualization method to study the killing mechanisms of PBQ and may help discover new chemicals that control snail populations.
基金Project supported by the National Natural Science Foundation of China(61565009,11664022,11804038)the Foundation of Natural Science of Yunnan Province(2016FB088)+3 种基金the Reserve Talents Project of Yunnan Province(2017HB011)the Young Talents Support Program of Faculty of Materials Science and Engineering,Kunming University of Science and Technology(14078342)Chongqing Natural Science Foundation(cstc2017jcyjAX0418,cstc2018jcyjAX0569)Foundation of Chongqing University of Arts and Sciences(R2016DQ10)
文摘Photo-stimulated luminescence(PSL) is the process in which trapped charges are released by photons and produce luminescence through recombination. The variegated optical characteristics of photostimulated phosphors(PSPs) have drawn increasing attention and a large body of work encompassing mechanism and application of PSPs has been addressed. The optical data storage capacity resulting from abundant defect states enables PSPs to be applied to information storage. Moreover, PSPs provide potential application for anti-counterfeiting, as color changes due to the tunneling process. Recently, near infrared(NIR) light PSPs have been developed, exhibiting enormous potential for in vivo bio-imaging, as the stable and high noise-signal ratio characteristic of PSL. In this review, we devote to introducing the development and process of PSPs, and the challenge and future advance have also been demonstrated.
基金supported by grants from the National Natural Science Foundation of China(Nos.81273451,81302717 and81101684)
文摘Nanomaterials with multiple functions have become more and more popular in the domain of cancer research. MoS2 has a great potential in photothermal therapy, X-ray/CT imaging and drug delivery. In this study, a water soluble MoS2 nanosystem(MoS2-PEG) was synthesized and explored in drug delivery, photothermal therapy(PTT) and X-ray imaging.Doxorubicin(DOX) was loaded onto MoS2-PEG with a high drug loading efficiency(~69%)and obtained a multifunctional drug delivery system(MoS2-PEG/DOX). As the drug delivery, MoS2-PEG/DOX could efficiently cross the cell membranes, and escape from the endosome via NIR light irradiation, lead to more apoptosis in MCF-7 cells, and afford higher antitumor efficacy without obvious toxic effects to normal organs owing to its prolonged blood circulation and 11.6-fold higher DTX uptake of tumor than DOX. Besides, MoS2-PEG/DOX not only served as a drug delivery system, but also as a powerful PTT agent for thermal ablation of tumor and a strong X-ray contrast agent for tumor diagnosis. In the in vitro and in vivo studies, MoS2-PEG/DOX exhibited excellent tumor-targeting efficacy, outstanding synergistic anti-cancer effect of photothermal and chemotherapy and X-ray imaging property,demonstrating that MoS2-PEG/DOX had a great potential for simultaneous diagnosis and photothermal-chemotherapy in cancer treatment.
基金supported by the grants from the National Natural Science Foundation of China(Nos.11174089 and 61138003)the Instrument Developing Project of the Chinese Academy of Sciences(No.YZ201263)+2 种基金the Instrument Function Developing Project of the Chinese Academy of Sciences(No.yg2012032)the Key Project of Department of Education of Guangdong Province(No.cxzd1112)Guangzhou Municipal Science and Technology Program Project(No.2012J5100004)
文摘Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes： （1） patterned illumination- based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; （2） single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluo- rophore at subdiffraction precision; （3） bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.
基金the National Natural Science Foundation of China(61722508/61525503/61620106016/61835009/61935012/61961136005)(Key)Project of Department of Education of Guangdong Province(2016KCXTD007)Shenzhen Basic Research Project(JCYJ20180305124902165).
文摘Nonlinear optics,which is a subject for studying the interaction between intense light and materials,has great impact on various research fields.Since many structures in biological tissues exhibit strong nonlinear optical effects,nonlinear optics has been widely applied in biomedical studies.Especially in the aspect of bio-imaging,nonlinear optical techniques can provide rapid,label-free and chemically specific imaging of biological samples,which enable the investigation of biological processes and analysis of samples beyond other microscopy techniques.In this review,we focus on the introduction of nonlinear optical processes and their applications in bio-imaging as well as the recent advances in this filed.Our perspective of this field is also presented.
基金supported by Health Technology Innovation Project of Jilin Province(No.2016J064)National Natural Science Foundation of China(No.81703136)
文摘Fluorescent organic nanoparticles(NPs)based on aggregation-induced emission(AIE)-active molecules,are widely applied as non-invasive tools in bio-imaging and demonstrate great potential for studying physiological and pathological processes.In this paper,we report the synthesis of highly emissive AIEgen-based NPs(SCA NPs)via reprecipitation without any carriers as long-term cell trackers.Uniformly sized SCA NPs enjoy the advantages of high brightness,good stability,large Stokes shift,good biocompatibility,and high photostability.The SCA NPs were successfully applied for in vitro bio-imaging of HeLa cells with an excellent cancer cell uptake.In addition,a strong fluorescence from SCA NPs can still be clearly observed in HeLa cells after incubation for six generations over 15 days.Thus,the SCA NPs could be ideal fluorescent probes for non-invasive long-term cellular imaging.The AIE-active NPs display superior performance and provide a basis for the development of fluorescent organic probes for monitoring biological processes.
文摘Natural resources are practically infinitely abundant in nature,which stimulates scientists to create new materials with inventive uses and minimal environmental impact.Due to the various benefits of natural carbon dots(NCDs)from them has received a lot of attention recently.Natural products-derived carbon dots have recently emerged as a highly promising class of nanomaterials,showcasing exceptional properties and eco-friendly nature,which make them appealing for diverse applications in various fields such as biomedical,environmental sensing and monitoring,energy storage and conversion,optoelectronics and photonics,agriculture,quantum computing,nanomedicine and cancer therapy.Characterization techniques such as Photoinduced electron transfer,Aggregation-Induced-Emission(AIE),Absorbance,Fluorescence in UV-Vis and NIR Regions play crucial roles in understanding the structural and optical properties of Carbon dots(CDs).The exceptional photoluminescence properties exhibited by CDs derived from natural products have paved the way for applications in tissue engineering,cancer treatment,bioimaging,sensing,drug delivery,photocatalysis,and promising remarkable advancements in these fields.In this review,we summarized the various synthesis methods,physical and optical properties,applications,challenges,future prospects of natural products-derived carbon dots etc.In this expanding sector,the difficulties and prospects for NCD-based materials research will also be explored.
基金The Fundamental Research Funds for the Central Universities,China(No.2232015D3-15)Shanghai Natural Science Foundation,China(No.14ZR1401300)“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)
文摘Novel CdTe/CdS quantum dots(QDs)coated with a hybrid of SiO_2 and ZnS were fabricated through a simple two-step approach.The hybrid SiO_2/ZnS coated CdTe/CdS quantum dots was characterized by transmission electron microscopy(TEM),UV and fluorescence spectrometer.Results indicated that the core-shell structure gave the QDs outstanding photoluminescence properties,includinganarrowphotoluminescencespectrum,high photoluminescence(PL)quantum yield and long emission lifetime(average PL lifetime of increased from 26.4 ns to 49.1 ns).Cellular studies showed the QDs had good cytocompatibility with Hela cells as determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)assay after coating SiO_2/ZnS,and also proved the feasibility of using the hybrid SiO_2/ZnS coated QDs as optical probes for in vitro cell imaging.The synthesis method of QDs is highly promising for the production of robust and functional optical probes for bio-imaging and sensing applications.
基金Supported by Qihuang Engineering High-Level Talent Team Cultivation Project of Guangxi University of Chinese Medicine(2018002)Guangxi Science and Technology Major Project(GK AA17292008)。
文摘Graphene is the thinest nanomaterial known in the world,which has unique electronic mobility,super specific surface area,high mechanical strength,excellent corrosion resistance and surface chemical structure.Due to its special nanostructure and excellent physical and chemical properties,graphene has a broad application prospect in the fields of electronics,optics,magnetism,biomedicine,catalysis,energy storage and sensors.In order to better develop and utilize graphene data,this paper reviewed the structural characteristics of graphene,as well as its research progress in biosensors,bio-imaging,aerogel and other biomedical fields,hoping to provide scientific basis for better development of graphene and the development of graphene pharmaceutical products.
文摘Background:Fluorescence bio-imaging in the second near-infrared window(NIR-II FL,1000-1700nm)has great potential in clinical theranostics,which is of great importance providing precise locations of lesions and molecular dynamic actions simultaneously in a single nanoprobe.Methods:T here has been an upsurge of multidisciplinary research focusing on developing functional types of inorganic and organic nanoprobes that can be used for NIR-II FL with the high spatiotemporal resolution,deep tissue penetration,and negligible auto-fluorescence.Results:In this mini-review,we summarize recent progress in inorganic/organic NIR-II FL nanoprobes.We introduce the design and properties of inorganic and organic nanoprobes,in the order of single-walled carbon nanotubes,quantum dots,rare-earth-doped nanoparticles,metal nanoclusters and organic fluorophores,expect to realize precise diagnosis and efficient image-guided therapy.Conclusion:Meanwhile,to elucidate the problems and perspectives,we aim to offer diverse biological applications of inorganic/organic NIR-II FL nanoprobes and accelerate the clinical transformation progress.
基金support from the National Natural Science Foundation of China(51925306,52120105006,52222309,52173187,and 52303221)the Strategic Priority Research Program of Chinese Academy of Sciences(XDB 0520103)+3 种基金Beijing Natural Science Foundation(Z210017)the Science and Technology Innovation Commission of Shenzhen(JCYJ20220530150604009)Project of International Cooperation of Chinese Academy of Sciences(124GJHZ2023079MI)Xiaomi Young Talents Program,and Fundamental Research Funds for the Central University.
文摘Poly-substituted olefins,one of the most important aggregation-induced emission luminogens(AIEgens),have garnered significant attention due to their various applications in chemical-and bio-sensing,bio-imaging,and opto-electronics.However,the synthetic methods for these olefins remain limited,impeding the progress of AIEgens.This study introduces an unprecedented cross-coupling reaction between aryl sulfonium triflates and tosylhydrazones from naturally abundant thioethers and ketones.The generality of this method is exemplified by the facile synthesis of over forty poly-substituted olefins.Importantly,the luminescent properties of these AIEgens(e.g.,quantum yield and emission color)can be easily tuned by adjusting the substituents of the electrophile and nucleophile substrates,exhibiting excellent performance in bio-imaging.Notably,the mechanistic studies reveal the critical role ofβ-H elimination in the formation of the double bond.This contribution provides an efficient method to synthesize poly-substituted olefins,pushing forward the development of AIEgens.
基金supported by UTS Science Seed Funding,UTS Chancellor’s Research Fellowship Program(No.PRO22-15457)the National Health and Medical Research Council(No.2025442).
文摘Luminescent nanoparticles(LNPs)have emerged as a promising approach for enhanced cell labelling and disease diagnosis by leveraging their unique photophysical and surface characteristics.Advanced generations of LNPs,such as quantum dots,dye-loaded nanoparticles and up-converting nanoparticles,exhibit distinct properties and advantages tailored for specialised applications.Consequently,there is a growing focus and demand to develop organelle-specific LNPs to identify,treat and elucidate disease mechanisms.The endoplasmic reticulum(ER)represents one such organelle,playing crucial roles in protein synthesis and modification,calcium homeostasis,lipid trafficking,and regulation of cellular stress.The unfolded protein response,regulated by ER stress,is a clinically significant pathway within the ER,implicated in cellular dysfunction and disease.The growing understanding of ER stress and the unfolded protein response has led to a rapid emergence of endoplasmic reticulum-targeting LNPs(ERLNPs)for precise intracellular diagnosis and therapy.This review discusses current advances and design principles of ERLNPs,highlights current achievements and applications,and discusses the challenges and interdisciplinarity needed for future development.
基金supported by the National Natural Science Foundation of China(Nos.21672185,22067019,82072309)the support of National Key R&D Program of China(No.2017YFC1200600)
文摘We constructed a reaction-based near-infrared fluorescent probe(Niap)to specifically identify alkaline phosphatase(ALP)with fast red fluorescence enhancement.Based on the positive concentrationdependent manner between the fluorescent intensity of the Niap and ALP,probe Niap was used to study the ALP enrichment and variation in golden apple snails(Pomacea canaliculata)exposed to the molluscicide candidate PPU06.After treatment with different concentrations of PPU06 over various times,three organs of the surviving snails,liver,stomach and plantaris,were frozen and sectioned for fluorescent imaging experiments.With increased PPU06 concentration,red fluorescence substantially increased in the liver and reached a maximum within 24 h when the PPU06 co ncentration was 0.75 mg/L.No obvious changes in the stomach or foot plantaris were found.It showed PPU06 caused liver injury and stimulated the increase of ALP in the liver of P.canaliculata.This study demonstrates a rapid ALP fluorescent identification method that can be used to study the effects of PPU06 on P.canaliculata.It also provides optical evidence that may aid in the discovery of new chemistry for snail control.
基金supported by the National Key Basic Research Program of China(Grant No.2014CB643802)the National Natural Science Foundation of China(Grant Nos.21371012,21321001,and 21621061)
文摘Luminescent lanthanide complexes have been widely investigated as light emitting materials in bio-imaging and sensing, solid state lighting and display, anti-fake tags and light conversion films, due to their characterized photophysical properties including large Stokes shift, long lifetime, and sharp emission spectrum, arising from the sensitized f-f transitions. In this review, we summarize the most recent advances in luminescent lanthanide complexes and their applications from 2015 to August 2017 concerning of general concepts to potential applications. We first introduce the basic concept of sensitized luminescence of lanthanide complexes and the strategies used for highly luminescent complexes. Then recent varieties of luminescent lanthanide complexes and their hybrid materials are presented. Finally, applications are discussed in detail.
基金the National Natural Science Foundation of China (No.21107083)Zhejiang Public Welfare Technology Research Project (No.LGF19H030014)Zhejiang Medical and Health Science & Technology Project (No.2018PY032).R.C.J.acknowledges the support from the AFOSR.
文摘Luminescent metal nanoclusters (NCs) have recently emerged as a novel class of luminescent nanomaterials and hold significant potential in biomedicine owing to their ultrasmall (<2nm) size,excellent photostability,and good biocompatibility.The recent rapid advances in the synthesis and functionalization of luminescent metal NCs have enabled scientists to develop colorful nanomaterials and nanodevices for a wide range of biomedical applications.In this review,we summarize the characteristics and advantages of luminescence from metal NCs,and highlight their applications in biomedicine.We focus on the research in biomedical detection,bio-imaging,drug delivery,and therapy,especially for the advances in the last five years.Luminescent metal NCs display a series of unique superiorities in biomedical applications,and the recent achievements have brought a lot of benefits to the diagnosis and treatment of clinical diseases,especially for tumors and cancers.Finally,we put forward the main challenges that currently still hinder the basic science studies and the practical development of luminescent metal NCs in biomedical applications.Overall,we expect that luminescent metal NCs will play a much more important role in future biomedicine and clinical applications.
基金This work was supported by Discovery Grants from the Australian Research Council(No.DP0879769).
文摘We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-vis)spectroscopy,photoluminescence(PL)spectroscopy,X-ray diffraction(XRD),and transmission electron microscopy(TEM)confirm that the CdTe QDs are formed via an extracellular growth and subsequent endocytosis pathway and have size-tunable optical properties with fluorescence emission from 490 to 560 nm and a cubic zinc blende structure with good crystallinity.In particular,the CdTe QDs with uniform size(2-3.6 nm)are protein-capped,which makes them highly soluble in water,and in situ bio-imaging in yeast cells indicates that the biosynthesized QDs have good biocompatibility.This work provides an economic and environmentally friendly approach to synthesize highly fluorescent biocompatible CdTe QDs for bio-imaging and bio-labeling applications.
