Citrus sinensis(L.)Osbeck fruit peels contain polyphenols which are known for its therapeutic properties.However,these polyphenols have limitations like astringent taste,low bioavailability,instability,and susceptibil...Citrus sinensis(L.)Osbeck fruit peels contain polyphenols which are known for its therapeutic properties.However,these polyphenols have limitations like astringent taste,low bioavailability,instability,and susceptibility to oxidation.To overcome these limitations,encapsulation techniques are being studied.Our study focuses on enriching polyphenols from Citrus sinensis fruit peels using amberlite XAD 16-N resin thereby developing efficient microspheres by extrusion technique using alginate matrix.Further,SEM and FTIR were used to characterise these microspheres.Additionally,it was also studied for swelling ability,polyphenols release at various pH,temperature,time,and bio-accessibility under simulated digestion condition.The results showed that the enriched polyphenols from Citrus sinensis fruit peels(EPCSP)were successfully encapsulated and maintained its stability significantly by retaining its total polyphenol content(63.08±2.2 mg/g of GAE)and total antioxidant activity(288.3±8.9 mg/g of AAE)in comparison to unencapsulated extract(p<0.05)throughout a period of 12 months.It was also observed that,at pH 7.4,the encapsulated microspheres showed significant swelling,resulting in an increased total polyphenol release than at pH 1.6.Furthermore,the EPCSP loaded alginate microspheres were also efficient in significantly maintaining the biological activities(p<0.05)thereby leading to a higher bio-accessibility index in the intestine.The present work highlighted the potential of alginate matrix which has shown improved stability of polyphenols and may be used as a drug delivery system which may lead to its wide applications in nutraceuticals/pharmaceutical industries.展开更多
The plant-based food market is expanding and demand for novel plant-based milk alternatives is increasing.Compared to conventional milk,lower protein content,and lower sensory acceptance are some of the challenges of ...The plant-based food market is expanding and demand for novel plant-based milk alternatives is increasing.Compared to conventional milk,lower protein content,and lower sensory acceptance are some of the challenges of the plant-based milk alternative industry.This research aimed to develop novel plant-based milk from two different local pistachio varieties grown in Antep and Siirt,Türkiye that would be higher in protein,unsaturated fatty acids,and phenolic compounds,and have superior taste and sensory characteristics.Protein content,fatty acid composition by gas chromatography,phenolic profile by high-pressure liquid chromatography,total phenolic content,and antioxidant activity by spectrophotometric method,and in vitro gastrointestinal digestion along with sensory characteristics by quantitative descriptive test were evaluated.The protein contents of the prepared pistachio milk were 3.78%and 3.09%for Antep and Siirt varieties of pistachio milk,respectively.The most abundant fatty acid was oleic acid and the most abundant phenolic compounds were catechin and gallic acid.Moreover,the bio-accessibilities of total phenolic content were 201%and 213%and the bio-accessibility of total antioxidant activity was 200%and 205%for plant-based milk of Antep and Siirt pistachio,respectively.The overall sensory acceptance scores of the prepared pistachio milk were 5.6-7/10.These findings indicated that plant-based milk substitutes prepared from Antep and Siirt pistachio were higher in protein,had highly bio-accessible phenolic content,and overall accepted in terms of sensory except color challenges.展开更多
Pterostilbene(Pte)is a dietary phenolic compound derived from plants that offers numerous physiological advantages.Nonetheless,its low water solubility and limited oral bioavailability restrict its bioactivity.To addr...Pterostilbene(Pte)is a dietary phenolic compound derived from plants that offers numerous physiological advantages.Nonetheless,its low water solubility and limited oral bioavailability restrict its bioactivity.To address these limitations,nanoparticles were prepared using anti-solvent precipitation method.Optimal nanoparticles were achieved at a 10:1 mass ratio of whey protein isolate(WPI)to Pte,resulting in a particle size of 104.54 nm,a polydispersity index of 0.42,and an impressive encapsulation efficiency of Pte at 85.02%.The water solubility of Pte in the nanoparticles was found to be 53.14 times higher than that of free Pte and remained stable under various environmental conditions.Furthermore,the DPPH and ABTS^(+)free radical scavenging activities of the nanoparticles were maximized at 55.58%and 92.97%,respectively.Pte release from WPI-Pte nanoparticles was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.This environmentally friendly,straightforward,and efficient approach shows promise for large-scale production of Pte products with amplified health benefits.展开更多
Clay minerals play an important role in biogeochemical cycling.Here,kaolinite and montmorillonite,the two most abundant and widespread clay minerals with typical layered structures,were selected to investigate and com...Clay minerals play an important role in biogeochemical cycling.Here,kaolinite and montmorillonite,the two most abundant and widespread clay minerals with typical layered structures,were selected to investigate and compare their effects on the biodegradation of benzo[a]pyrene(BaP)by Paracoccus aminovorans HPD-2 and to investigate the underlying interface mechanisms.Overall,the BaP degradation efficiency was significantly higher 7 d after montmorillonite addition,reaching 68.9%(P<0.05),when compared with that of the control without addition of clay minerals(CK,61.4%);however,the addition of kaolinite significantly reduced the BaP degradation efficiency to 45.8%.This suggests that kaolinite inhibits BaP degradation by inhibiting the growth of strain HPD-2,or its strong hydrophobicity and readily agglomerates in the degradation system,resulting in a decrease in the bio-accessibility of BaP to strain HPD-2.Montmorillonite may buffer some unfavorable factors,and cells may be fixed on the surface of montmorillonite colloidal particles across energy barriers.Furthermore,the adsorption of BaP on montmorillonite may be weakened after swelling,reducing the effect on the bio-accessibility of BaP,thus promoting the biodegradation of BaP by strain HPD-2.The experimental results indicate that differential bacterial growth,BaP bio-accessibility,interface interaction,and the buffering effect may explain the differential effects of the different minerals on polycyclic aromatic hydrocarbon biodegradation.These observations improve our understanding of the mechanisms by which clay minerals,organic pollutants,and degrading bacteria interact during the biodegradation process and provide a theoretical basis for increasing the biodegradation of soil pollutants by native microorganisms under field conditions.展开更多
The aim of the study was to investigate the effect of bread fortification with dry olive paste flour(DOPF),coming from Cellina di Nardòcultivar.Specifically,sensory and nutritional properties of enriched bread we...The aim of the study was to investigate the effect of bread fortification with dry olive paste flour(DOPF),coming from Cellina di Nardòcultivar.Specifically,sensory and nutritional properties of enriched bread were investigated.In addition,the glycemic response and the bio-accessibility of polyphenols contained in the bread samples were also estimated.The addition of DOPF leads to an increase of the total phenolic compounds,total flavonoids and antioxidant activity,without compromising the final acceptability.The recovery of bio-accessible polyphenols from the simulated gastro-intestinal digestion was 59.3%for the control and 72.1%for the enriched bread.Also the glycemic response was improved in the enriched bread.展开更多
Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their ...Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their application in nutraceuticals.To alleviate these constraints,effective encapsulation techniques have been developed.This study aimed to enrich polyphenols(EP)from Desmodium gangeticum leaves using Amberlite XAD-16 resin and to develop EP microspheres by extrusion method using sodium alginate as matrix.Further to assess impact of alginate encapsulation on stability of total polyphenol content,antioxidant activity and biological properties for 12-month duration.The encapsulated EP microspheres were characterized by SEM and FTIR.The encapsulated EP microspheres were also assessed for their swelling characteristics,release of polyphenols at different pH,temperatures,and time durations,and bio-accessibility by in vitro simulated gastrointestinal digestion.The results showed that EP were successfully encapsulated using extrusion method.The TPC(67.8±2.6 mg/g of GAE),antioxidant activity(177.2±4.3 mg/g of AAE)of encapsulated EP microspheres were significantly stable(p<0.05)throughout 12-month duration compared to unencapsulated EP.The encapsulated EP microspheres at pH 7.4 exhibited maximum swelling,leading to a higher release of TPC compared to pH 1.6.The bio-accessibility of encapsulated EP was increased during simulated gastrointestinal conditions.The bio-logical activities of encapsulated EP microspheres also significantly retained the stability(p<0.05)compared to unencapsulated EP for 12-month duration.The results supported that utilizing alginate matrix for encapsulation improves stability and may be a promising method for food supplementation containing natural antioxidants.展开更多
We used LC-MS to identify the metabolic changes in soybean fermented by Bacillus subtilis DC-15 to comprehend the biochemical alterations.Total 650 distinct metabolites were observed,with amino acids,dipeptides,fatty ...We used LC-MS to identify the metabolic changes in soybean fermented by Bacillus subtilis DC-15 to comprehend the biochemical alterations.Total 650 distinct metabolites were observed,with amino acids,dipeptides,fatty acids,small molecule sugars,and isoflavones having the largest abundances.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that the primary metabolic pathways involved during the fermentation of soybeans were related to amino acid metabolism,including beta-alanine metabolism,phenylalanine metabolism,glycine,serine,and threonine metabolism.In the gastric phase,the maximum amount of soy isoflavones was successfully converted from its glycoside form(50.04μg/mL)to the aglycone form(104.07μg/mL).The distribution of protein and lipid particles in natto during various stages of digestion showed changes from aggregated particles to more scattered particles.For the future development and enhancement of fermented soybean products,our metabolomic data can shed light on activated biochemical pathways and changes in metabolic pattern.展开更多
文摘Citrus sinensis(L.)Osbeck fruit peels contain polyphenols which are known for its therapeutic properties.However,these polyphenols have limitations like astringent taste,low bioavailability,instability,and susceptibility to oxidation.To overcome these limitations,encapsulation techniques are being studied.Our study focuses on enriching polyphenols from Citrus sinensis fruit peels using amberlite XAD 16-N resin thereby developing efficient microspheres by extrusion technique using alginate matrix.Further,SEM and FTIR were used to characterise these microspheres.Additionally,it was also studied for swelling ability,polyphenols release at various pH,temperature,time,and bio-accessibility under simulated digestion condition.The results showed that the enriched polyphenols from Citrus sinensis fruit peels(EPCSP)were successfully encapsulated and maintained its stability significantly by retaining its total polyphenol content(63.08±2.2 mg/g of GAE)and total antioxidant activity(288.3±8.9 mg/g of AAE)in comparison to unencapsulated extract(p<0.05)throughout a period of 12 months.It was also observed that,at pH 7.4,the encapsulated microspheres showed significant swelling,resulting in an increased total polyphenol release than at pH 1.6.Furthermore,the EPCSP loaded alginate microspheres were also efficient in significantly maintaining the biological activities(p<0.05)thereby leading to a higher bio-accessibility index in the intestine.The present work highlighted the potential of alginate matrix which has shown improved stability of polyphenols and may be used as a drug delivery system which may lead to its wide applications in nutraceuticals/pharmaceutical industries.
文摘The plant-based food market is expanding and demand for novel plant-based milk alternatives is increasing.Compared to conventional milk,lower protein content,and lower sensory acceptance are some of the challenges of the plant-based milk alternative industry.This research aimed to develop novel plant-based milk from two different local pistachio varieties grown in Antep and Siirt,Türkiye that would be higher in protein,unsaturated fatty acids,and phenolic compounds,and have superior taste and sensory characteristics.Protein content,fatty acid composition by gas chromatography,phenolic profile by high-pressure liquid chromatography,total phenolic content,and antioxidant activity by spectrophotometric method,and in vitro gastrointestinal digestion along with sensory characteristics by quantitative descriptive test were evaluated.The protein contents of the prepared pistachio milk were 3.78%and 3.09%for Antep and Siirt varieties of pistachio milk,respectively.The most abundant fatty acid was oleic acid and the most abundant phenolic compounds were catechin and gallic acid.Moreover,the bio-accessibilities of total phenolic content were 201%and 213%and the bio-accessibility of total antioxidant activity was 200%and 205%for plant-based milk of Antep and Siirt pistachio,respectively.The overall sensory acceptance scores of the prepared pistachio milk were 5.6-7/10.These findings indicated that plant-based milk substitutes prepared from Antep and Siirt pistachio were higher in protein,had highly bio-accessible phenolic content,and overall accepted in terms of sensory except color challenges.
基金supported by the National Natural Science Foundation of China(No.32101990,No.32372475,No.32130084)National Key Research and Development Program of China-China(No.2022YFF1100103)+1 种基金the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University(2022-1-1)China Postdoctoral Science Foundation(2023M731142).
文摘Pterostilbene(Pte)is a dietary phenolic compound derived from plants that offers numerous physiological advantages.Nonetheless,its low water solubility and limited oral bioavailability restrict its bioactivity.To address these limitations,nanoparticles were prepared using anti-solvent precipitation method.Optimal nanoparticles were achieved at a 10:1 mass ratio of whey protein isolate(WPI)to Pte,resulting in a particle size of 104.54 nm,a polydispersity index of 0.42,and an impressive encapsulation efficiency of Pte at 85.02%.The water solubility of Pte in the nanoparticles was found to be 53.14 times higher than that of free Pte and remained stable under various environmental conditions.Furthermore,the DPPH and ABTS^(+)free radical scavenging activities of the nanoparticles were maximized at 55.58%and 92.97%,respectively.Pte release from WPI-Pte nanoparticles was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.This environmentally friendly,straightforward,and efficient approach shows promise for large-scale production of Pte products with amplified health benefits.
基金supported by the grants from Chinese Academy of Sciences(CAS)Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science,CAS,the Natural Science Foundation of Jiangsu Province,China(No.BK20150049)the Chinese National Key Research and Development Program(Nos.2017YFA0207001 and 2019YFC1803700)。
文摘Clay minerals play an important role in biogeochemical cycling.Here,kaolinite and montmorillonite,the two most abundant and widespread clay minerals with typical layered structures,were selected to investigate and compare their effects on the biodegradation of benzo[a]pyrene(BaP)by Paracoccus aminovorans HPD-2 and to investigate the underlying interface mechanisms.Overall,the BaP degradation efficiency was significantly higher 7 d after montmorillonite addition,reaching 68.9%(P<0.05),when compared with that of the control without addition of clay minerals(CK,61.4%);however,the addition of kaolinite significantly reduced the BaP degradation efficiency to 45.8%.This suggests that kaolinite inhibits BaP degradation by inhibiting the growth of strain HPD-2,or its strong hydrophobicity and readily agglomerates in the degradation system,resulting in a decrease in the bio-accessibility of BaP to strain HPD-2.Montmorillonite may buffer some unfavorable factors,and cells may be fixed on the surface of montmorillonite colloidal particles across energy barriers.Furthermore,the adsorption of BaP on montmorillonite may be weakened after swelling,reducing the effect on the bio-accessibility of BaP,thus promoting the biodegradation of BaP by strain HPD-2.The experimental results indicate that differential bacterial growth,BaP bio-accessibility,interface interaction,and the buffering effect may explain the differential effects of the different minerals on polycyclic aromatic hydrocarbon biodegradation.These observations improve our understanding of the mechanisms by which clay minerals,organic pollutants,and degrading bacteria interact during the biodegradation process and provide a theoretical basis for increasing the biodegradation of soil pollutants by native microorganisms under field conditions.
文摘The aim of the study was to investigate the effect of bread fortification with dry olive paste flour(DOPF),coming from Cellina di Nardòcultivar.Specifically,sensory and nutritional properties of enriched bread were investigated.In addition,the glycemic response and the bio-accessibility of polyphenols contained in the bread samples were also estimated.The addition of DOPF leads to an increase of the total phenolic compounds,total flavonoids and antioxidant activity,without compromising the final acceptability.The recovery of bio-accessible polyphenols from the simulated gastro-intestinal digestion was 59.3%for the control and 72.1%for the enriched bread.Also the glycemic response was improved in the enriched bread.
文摘Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their application in nutraceuticals.To alleviate these constraints,effective encapsulation techniques have been developed.This study aimed to enrich polyphenols(EP)from Desmodium gangeticum leaves using Amberlite XAD-16 resin and to develop EP microspheres by extrusion method using sodium alginate as matrix.Further to assess impact of alginate encapsulation on stability of total polyphenol content,antioxidant activity and biological properties for 12-month duration.The encapsulated EP microspheres were characterized by SEM and FTIR.The encapsulated EP microspheres were also assessed for their swelling characteristics,release of polyphenols at different pH,temperatures,and time durations,and bio-accessibility by in vitro simulated gastrointestinal digestion.The results showed that EP were successfully encapsulated using extrusion method.The TPC(67.8±2.6 mg/g of GAE),antioxidant activity(177.2±4.3 mg/g of AAE)of encapsulated EP microspheres were significantly stable(p<0.05)throughout 12-month duration compared to unencapsulated EP.The encapsulated EP microspheres at pH 7.4 exhibited maximum swelling,leading to a higher release of TPC compared to pH 1.6.The bio-accessibility of encapsulated EP was increased during simulated gastrointestinal conditions.The bio-logical activities of encapsulated EP microspheres also significantly retained the stability(p<0.05)compared to unencapsulated EP for 12-month duration.The results supported that utilizing alginate matrix for encapsulation improves stability and may be a promising method for food supplementation containing natural antioxidants.
基金supported by the National Key Research&Development Program of China(2022YFF1100305)the National Natural Science Foundation of China(31850410476)+1 种基金the National Natural Science Foundation of Ningxia Province(2022AAC03230)the Researchers Supporting Project King Saud University(Riyadh,Saudi Arabia)(RSP2024R138).
文摘We used LC-MS to identify the metabolic changes in soybean fermented by Bacillus subtilis DC-15 to comprehend the biochemical alterations.Total 650 distinct metabolites were observed,with amino acids,dipeptides,fatty acids,small molecule sugars,and isoflavones having the largest abundances.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that the primary metabolic pathways involved during the fermentation of soybeans were related to amino acid metabolism,including beta-alanine metabolism,phenylalanine metabolism,glycine,serine,and threonine metabolism.In the gastric phase,the maximum amount of soy isoflavones was successfully converted from its glycoside form(50.04μg/mL)to the aglycone form(104.07μg/mL).The distribution of protein and lipid particles in natto during various stages of digestion showed changes from aggregated particles to more scattered particles.For the future development and enhancement of fermented soybean products,our metabolomic data can shed light on activated biochemical pathways and changes in metabolic pattern.
