The survival adaptation of bacteria in saline soil is poor.The bilayer microcapsules were prepared by secondary embedding of monolayer sodium alginate(NaAlg)-bentonite(Bent)-sodium carboxy-methylcellulose(CMC)microcap...The survival adaptation of bacteria in saline soil is poor.The bilayer microcapsules were prepared by secondary embedding of monolayer sodium alginate(NaAlg)-bentonite(Bent)-sodium carboxy-methylcellulose(CMC)microcapsules wrapped with plant growth promoting rhizobacteria(PGPR)Pseudomonas putida Rs-198 by chitosan solution to promote the synergistic effect of bilayer microen-capsulation and PGPR.The characterization of the Rs-198 bilayer microcapsules showed that the amino and carboxyl groups were cross-linked and a thin layer of chitosan was formed on the outside of the microcapsule.The bilayer microcapsule(Ch-d)with a chitosan concentration of 0.8 wt%and pH 6 showed a slow release of bacteria with a maximum release of 6.06 × 10^(9) cfu/g on the 7th day.The viable bacteria of Ch-d increased by 4.42%after 60 days of storage compared with monolayer microcapsules.The 0.9 wt%L-cysteine,10 wt%glycerinum,10 wt%trehalose and 12 wt%soluble starch were added as bacterial protective agents during the process of preparing the Ch-d lyophilized bacterial inoculant(Ch-d LBI).Pot experiments showed that Ch-d LBI exhibited better growth promotion of Capsicum annuum L.under salt stress.Therefore,the bilayer microcapsule as slow-release bacterial inoculant is a potential alternative for sustainable agriculture.展开更多
基金supported by the National Natural Science Foundation of China(grant No.U1803332,22278325)Xi'an Science and Technology Plan Project Agricultural Technology R&D Project(grant No.21NYYF0030,22NYYF037)+2 种基金the Scientific Research Plan for Local Special Service of Shaanxi Provincial Education Department(grant No.20JC014)Preferential Funding Projects for Scientific and Technological Activities of Overseas Scholar(grant No.2020018)Key Research and Development Program of Xianyang City(grant No.S2021ZDYF-NY-0024)。
文摘The survival adaptation of bacteria in saline soil is poor.The bilayer microcapsules were prepared by secondary embedding of monolayer sodium alginate(NaAlg)-bentonite(Bent)-sodium carboxy-methylcellulose(CMC)microcapsules wrapped with plant growth promoting rhizobacteria(PGPR)Pseudomonas putida Rs-198 by chitosan solution to promote the synergistic effect of bilayer microen-capsulation and PGPR.The characterization of the Rs-198 bilayer microcapsules showed that the amino and carboxyl groups were cross-linked and a thin layer of chitosan was formed on the outside of the microcapsule.The bilayer microcapsule(Ch-d)with a chitosan concentration of 0.8 wt%and pH 6 showed a slow release of bacteria with a maximum release of 6.06 × 10^(9) cfu/g on the 7th day.The viable bacteria of Ch-d increased by 4.42%after 60 days of storage compared with monolayer microcapsules.The 0.9 wt%L-cysteine,10 wt%glycerinum,10 wt%trehalose and 12 wt%soluble starch were added as bacterial protective agents during the process of preparing the Ch-d lyophilized bacterial inoculant(Ch-d LBI).Pot experiments showed that Ch-d LBI exhibited better growth promotion of Capsicum annuum L.under salt stress.Therefore,the bilayer microcapsule as slow-release bacterial inoculant is a potential alternative for sustainable agriculture.
