Seaweed was fermented using Bifidobacterium,and its high-density fermentation conditions were investigated through single-factor experiments.The optimized fermentation conditions were determined as follows:Ulva powder...Seaweed was fermented using Bifidobacterium,and its high-density fermentation conditions were investigated through single-factor experiments.The optimized fermentation conditions were determined as follows:Ulva powder as the fermentation algal body,Ulva powder addition at 2%,fermented nitrogen source as 1%soy peptone,fermentation time of 26 h,and fermentation temperature of 37℃.The Ulva powder ferment broth was subjected to the Hen’s Egg Test-Chorioallantoic Membrane(HET-CAM)assay and acute cell toxicity testing to evaluate its irritancy and safety.Additionally,cell-based experiments for firming and anti-wrinking efficacy,soothing effect,whitening potency,and antioxidant activity were conducted to assess its cosmetic functionalities.The results indicated that the obtained Ulva powder ferment broth was non-irritating and exhibited a high non-cytotoxic concentration.A 5%concentration of the Ulva powder ferment broth promoted the upregulation of Type Ⅰ collagen by 32.58%±1.33%.A 10%concentration of the ferment broth achieved relative inhibition rates of 67.64%±3.99%for nitric oxide(NO)and 55.76%±0.11%for melanin,respectively.Furthermore,a 6.25%concentration of the ferment broth demonstrated an ABTS radical scavenging rate of 58.29%±4.92%.These findings suggest that the Ulva powder ferment broth is a fermentation product characterized by high safety,excellent anti-wrinking,soothing,and whitening efficacies,along with potent in vitro antioxidant capacity.This study demonstrates its value-added development and significant potential for application in industries such as cosmetics and food.展开更多
Ulva lactuca whole components served as a carbon source for high-density fermentation of Bifidobacterium,yielding a low-cost,highly biocompatible Ulva lactuca bifidobacterium ferment filtrate(ULBFF).Its peptide profil...Ulva lactuca whole components served as a carbon source for high-density fermentation of Bifidobacterium,yielding a low-cost,highly biocompatible Ulva lactuca bifidobacterium ferment filtrate(ULBFF).Its peptide profile and molecular weight distribution were characterized by LC/MS.Cosmetic potential was systematically evaluated through stability testing,HET-CAM test,ABTS+∙/DPPH∙scavenging,NO inhibition,melanin suppression,and type Ⅰ collagen promotion.The results indicated a rich distribution of peptides,predominantly low-molecular-weight oligopeptides,with 92.92%of peptides containing≤20 amino acids and 84.89%of components having a molecular weight<500 Da.Formulations exhibited excellent stability and low irritation.Furthermore,they showed significantly enhanced efficacy in key areas,including antioxidant and antiinflammatory activity,melanin suppression,and collagen promotion.The ULBFF improved multifunctional performance while maintaining formulation stability and safety,demonstrating high scalability and potential for marine bioresource utilization in functional cosmetics.展开更多
Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a n...Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a natural physical barrier against external interference.Our previous study established a dynamic culture system of the biofilm-state Bifidobacterium adolescentis Gr19(B-DC-B.adolescentis Gr19),forming higher density and more structurally stable biofilms,which enhanced its potential probiotic properties in vivo.Thus,the protective effect and mechanism of B-DC-B.adolescentis Gr19 on lipopolysaccharide(LPS)-induced intestinal barrier dysfunction were investigated in this study.The results showed that B-DC-B.adolescentis Gr19 not only had high resistance and adhesion activity,but also improved the intestinal barrier by increasing goblet cells and promoting the expression of tight junction(TJ)-related proteins.Moreover,B-DC-B.adolescentis Gr19 effectively attenuated intestinal barrier injury in Caco-2 cells by improving intestinal permeability and integrity.Remarkably,B-DC-B.adolescentis Gr19 enhanced expression of TJ proteins,restored localization of cytoskeleton and reduced intestinal inflammation by suppressing the Ras homolog family member A/Rho-associated coiled-coil-forming kinases/nuclear factor kappa B/myosin light chain kinase/myosin light chain(RhoA/ROCK/NF-κB/MLCK/MLC)pathway.Therefore,B-DC-B.adolescentis Gr19 plays a key role in mitigating LPS-induced intestinal barrier dysfunction.Overall,the present study provides a theoretical basis for ameliorating intestinal barrier dysfunction and developing novel functional foods by using biofilm-state probiotics under dynamic culture.展开更多
This study aimed to investigate the effects of infant feces-derived Bifidobacterium breve CCFM1078 on rheumatoid cachexia(RC).Twenty-four female Wistar rats were assigned to 3 groups:CON group(normal saline by gavage)...This study aimed to investigate the effects of infant feces-derived Bifidobacterium breve CCFM1078 on rheumatoid cachexia(RC).Twenty-four female Wistar rats were assigned to 3 groups:CON group(normal saline by gavage),CIA group(collagen-induced arthritis(CIA),normal saline by gavage),and CCFM1078 group(CIA,3×10^(9)CFU/(rat·day)B.breve CCFM1078 gavage).The results demonstrated that B.breve CCFM1078 not only improved skeletal muscle function in CIA rats,but also modulated the gut microbiota,skeletal muscle metabolism and hormone levels,reduced inflammation in the knee joint and skeletal muscles,decreased activity of the nuclear factor κB(NF-κB)inflammatory signaling pathway,enhanced the insulin receptor substrate 1(IRS1)/phosphatidylinositol 3-kinase/protein kinase(PI3K/Akt)signaling pathway,promoted skeletal muscle differentiation,and maintained skeletal muscle fiber diameter,consequently slowing down the progression of RC.These findings suggested that B.breve CCFM1078 may have a beneficial role as part of a dietary intervention for RC,enhancing overall therapeutic effects.展开更多
脂代谢紊乱是肥胖及代谢综合征的核心诱因,利用益生菌调控肠道菌群已成为营养干预的重要策略。本研究以斑马鱼为模型,评价了Bifidobacterium breve 207-1(B.breve 207-1)及其灭活形态对脂代谢的调节作用。结果表明,二者均能通过调控ucp...脂代谢紊乱是肥胖及代谢综合征的核心诱因,利用益生菌调控肠道菌群已成为营养干预的重要策略。本研究以斑马鱼为模型,评价了Bifidobacterium breve 207-1(B.breve 207-1)及其灭活形态对脂代谢的调节作用。结果表明,二者均能通过调控ucp1等关键基因,促进脂肪分解与吸收。在降血脂方面,活菌特异性降低胆固醇,而灭活菌能同时降低胆固醇与甘油三酯,展现出更广谱的稳定功效,为后生元功能食品开发提供了新思路。展开更多
Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration s...This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration saline)and the CCFM1078 group(administration B.breve CCFM1078,3×10^(9) CFU/day)in 3-,4-,and 5-week tests.All the groups have male and female distinctions.Our findings demonstrate that B.breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites(hexadecanamide,linoleoyl ethanolamide,and palmitoyl ethanolamide),the genes and proteins expression related to the growth hormone(GH)/insulin-like growth factors-1(IGF-1)axis and Gs/PKA/CREB signaling pathways,as well as downstream osteogenic and osteoclastic differentiation factors.The effects of B.breve CCFM1078 were different with age and gender dependent.This finding suggests B.breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.展开更多
The impact of mother-infant microbiota on neurodevelopment is an area of interest,but longitudinal studies are scarce.Using a cohort of 520 families from the Jiangsu birth cohort in China,we reveal that the maternal g...The impact of mother-infant microbiota on neurodevelopment is an area of interest,but longitudinal studies are scarce.Using a cohort of 520 families from the Jiangsu birth cohort in China,we reveal that the maternal gut microbiota during early pregnancy play a substantial role,accounting for 3.34%of the variance in offspring neurodevelopmental scores.This contribution is notably higher than the 1.24%attributed to the infants’own microbiota at 1 year of age,underscoring the significant influence of maternal gut health on early child development.Remarkably,an elevation in maternal Bifidobacterium pseudocatenulatum is linked to decreased cognitive scores,whereas an enrichment of Bifidobacterium longum at 1 year of age is associated with higher cognitive scores.Furthermore,we find that maternal B.pseudocatenulatum is linked to the heterolactic fermentation metabolic pathway,while infant B.longum is associated with the Bifidobacterium shunt pathway.In summary,our analysis implies that maternal and infant gut microbiota play a distinct role in neurodevelopment,suggesting potential strategies for improving neurodevelopmental outcomes during early pregnancy or infant development by targeting gut microbiota composition.展开更多
This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mir...This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mirtazapine in treating postoperative depression of gastric cancer patients,also explored the multi-target therapeutic mechanisms of Bifidobacterium triple viable bacteria combined with mirtazapine in managing depression in patients after radical gastrectomy for gastric cancer for the first time.The results indicated that Bifidobacterium could alleviate depression in patients after radical gastrectomy for gastric cancer.Gastric cancer patients undergoing surgery frequently experience postoperative psychological disorders,notably depression.These conditions not only impair quality of life but also negatively impact disease-free and overall survival.This editorial explores the underestimated psychological challenges following gastric cancer surgery,including depression,anxiety,adjustment disorders,and post-traumatic stress disorder.It further examines the therapeutic potential of Bifidobacterium triple viable probiotics,combined with mirtazapine,in alleviating postoperative depression through microbiological,immunological,and neurological mechanisms.Clinical evidence highlights additional benefits,such as improved gastrointestinal function,enhanced immunity,and better nutritional status,underscoring a holistic approach to postoperative care.While promising,further large-scale trials are needed to optimize and personalize probiotic-based interventions.展开更多
Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to all...Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to alleviate obesity by modulating the microbiota.In this study,we found that oral administration of Bifidobacterium adolescentis CCFM8630 to obese mice inhibited high-fat diet(HFD)-induced changes in body weight and adipose tissue and alleviated hepatic oxidative stress.Furthermore,B.adolescentis CCFM8630 treatment primarily affected the relative abundances of the phyla Proteobacteria and Actinobacteria,and thereby decreased the production of lipopolysaccharide(LPS)and the occurrence of LPS related diseases.A high fiber intake increased the abundance of Lactobacillus and the concentrations of short chain fatty acids in obese mice,but these changes were reversed by B.adolescentis CCFM8630 treatment.In addition,targeted metabolomic analysis and microbiota relationship analysis revealed that B.adolescentis CCFM8630 treatment modified the microbiota of obese mice by promoting the conversion of tryptophan(Trp)to xanthurenic acid,kynurenic acid,tryptamine,indole-3-acetic acid,and indole-3-carboxaldehyde;facilitated the expression of interleukin-17A and the aryl hydrocarbon receptor to generate interleukin-22 in the colon;and upregulated the expression of tight junction proteins,thereby strengthening intestinal barriers.In summary,our findings suggest that the intake of B.adolescentis CCFM8630 may alleviate obesity by modulating the gut microbiota and related Trp metabolism.展开更多
BACKGROUND Patients with gastric cancer may have a risk of depression after radical surgery,which affects recovery and requires intensive intervention.AIM To explore the role of Bifidobacterium triple viable bacteria-...BACKGROUND Patients with gastric cancer may have a risk of depression after radical surgery,which affects recovery and requires intensive intervention.AIM To explore the role of Bifidobacterium triple viable bacteria-assisted mirtazapine in patients with depression after radical surgery for gastric cancer.METHODS This study included 80 patients with gastric cancer who experienced depression after undergoing radical surgery from January 2022 to December 2023 at Jiangnan University Hospital in Wuxi city in Jiangsu province.The patients were categorized into the control group(n=40)treated with mirtazapine and observation group(n=40)treated with combined Bifidobacterium triple viable bacteria-assisted mirtazapine.Neuroendocrine index,intestinal flora level,nutrition status,and patient quality of life were analyzed.RESULTS Before drug index level,no statistically significant difference was observed between the two groups (P>0.05).After drug administration,both groups experienced a decline in depression scores,cortisol,Escherichia coli,Enterococcus faecalis levels,and quality of life scores,whereas dopamine,serotonin,Lactobacillus,Bifidobacteria,hemoglobin,serum albumin and prealbumin levels increased.The observation group demonstrated significantly greater improvements across these indicators,with significant differences within groups and between groups(P<0.05).CONCLUSION Bifidobacterium has a relieving effect on depression in patients after radical surgery for gastric cancer,improves neuroendocrine status,regulates intestinal flora,and improves nutritional status and quality of life.展开更多
Aflatoxin B_1(AFB_1)is a common contaminant in cereals of global concern,and long-term low-dose exposure can adversely affect human health.Here,we showed that populations with dietary patterns characterized by high-fa...Aflatoxin B_1(AFB_1)is a common contaminant in cereals of global concern,and long-term low-dose exposure can adversely affect human health.Here,we showed that populations with dietary patterns characterized by high-fat diet(HFD)might have an increased risk of exposure to high levels of AFB_1.Our data indicated that chronic exposure of AFB_1 induced“gut-liver axis”injury in mice under HFD and normal diet(ND)patterns.AFB_1 further aggravated hepatic and intestinal injury,and intestinal microbiota disruption in HFD mice.Bifidobacterium breve BAA-2849 intervention analysis showed that liver injury and lipid disorders caused by AFB_1 exposure were alleviated by regulating the proportions of different gut microbes.We demonstrated through a mice model that the populations with a dietary pattern of HFD might be more susceptible to AFB_1 exposure and adverse effects on the gut-liver axis,and the toxicity of AFB_1 exposure can be alleviated by regulating the gut microbiota.展开更多
Objective:To analyze the efficacy of Bifidobacterium triple viable bacteria tablets on neonatal necrotizing enterocolitis(NEC)and its impact on serum factors of the patients.Methods:From January 2021 to May 2025,88 ne...Objective:To analyze the efficacy of Bifidobacterium triple viable bacteria tablets on neonatal necrotizing enterocolitis(NEC)and its impact on serum factors of the patients.Methods:From January 2021 to May 2025,88 neonates with NEC admitted to our hospital were selected as study subjects.During the study,these 88 patients were evenly divided into two groups,namely the observation group and the control group,with 44 patients in each group based on the random number table method.In terms of treatment,the control group was treated with meropenem,while the observation group received additional treatment with Bifidobacterium triple viable bacteria powder based on the treatment plan of the control group.The clinical efficacy and differences in serum inflammatory factor levels between the two groups were compared.Results:The efficacy of the observation group(90.91%)was better than that of the control group(72.73%)(P<0.05).After treatment,the levels of C-reactive protein(CRP)and procalcitonin(PCT)in both groups decreased compared to those before treatment,and the values of the above indicators in the observation group were lower than those in the control group(P<0.05).Conclusion:Based on conventional treatment for NEC neonates,the use of Bifidobacterium triple viable bacteria tablets has significant efficacy and can effectively reduce serum inflammatory factor levels.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
Objective： To construct Bifidobacterium Infantis/CD targeting gene therapy system. Methods： CD gene was amplified from E. Coli K12λ using PCR method, pGEX-1LamdaT plasmid and CD gene were digested with dual restric...Objective： To construct Bifidobacterium Infantis/CD targeting gene therapy system. Methods： CD gene was amplified from E. Coli K12λ using PCR method, pGEX-1LamdaT plasmid and CD gene were digested with dual restriction endonucleas of EcoR Ⅰ and BamH Ⅰ and two segments of 4.9 kb and 1.3 kb were obtained. T4 DNA ligase was added to these two segments to make a recombinant CD/pGEX-1LamdaT plasmid. Then the recombinant plasmid was transfected into Bifidobacterium Infantis by electroporation. The recombinant plasmid was extracted from the positively transfected Bifidobacterium Infantis and digested with dual restriction endonucleases. Then the size of digested fragments was detected and sequencing of the gene segment inserted in extracted recombinant plasmid was performed according to the method of Sanger dideoxynucleotide triphosphate chain termination. Results： 6.2 kb recombinant plasmid was obtained from the positively transfected bacterial colony of Bifidobacterium Infantis. After being digested with dual restriction endonucleases, two segments of approximate 4.9 kb and 1.3 kb were gained from the extracted recombinant plasmid, which were equal to the size of pGEX-1LamdaT plasmid and CD gene, respectively. The full length and sequence of nucleotide acid of the inserted gene in extracted recombinant plasmid was completely identical to the CD gene. Conclusion： The foreign gene, CD gene was correctly inserted into pGEX-1LambdaT plasmid and transferred into Bifidobacterium Infantis. Bifidobacterium Infantis/CD targeting gene therapy system was successfully constructed.展开更多
文摘Seaweed was fermented using Bifidobacterium,and its high-density fermentation conditions were investigated through single-factor experiments.The optimized fermentation conditions were determined as follows:Ulva powder as the fermentation algal body,Ulva powder addition at 2%,fermented nitrogen source as 1%soy peptone,fermentation time of 26 h,and fermentation temperature of 37℃.The Ulva powder ferment broth was subjected to the Hen’s Egg Test-Chorioallantoic Membrane(HET-CAM)assay and acute cell toxicity testing to evaluate its irritancy and safety.Additionally,cell-based experiments for firming and anti-wrinking efficacy,soothing effect,whitening potency,and antioxidant activity were conducted to assess its cosmetic functionalities.The results indicated that the obtained Ulva powder ferment broth was non-irritating and exhibited a high non-cytotoxic concentration.A 5%concentration of the Ulva powder ferment broth promoted the upregulation of Type Ⅰ collagen by 32.58%±1.33%.A 10%concentration of the ferment broth achieved relative inhibition rates of 67.64%±3.99%for nitric oxide(NO)and 55.76%±0.11%for melanin,respectively.Furthermore,a 6.25%concentration of the ferment broth demonstrated an ABTS radical scavenging rate of 58.29%±4.92%.These findings suggest that the Ulva powder ferment broth is a fermentation product characterized by high safety,excellent anti-wrinking,soothing,and whitening efficacies,along with potent in vitro antioxidant capacity.This study demonstrates its value-added development and significant potential for application in industries such as cosmetics and food.
文摘Ulva lactuca whole components served as a carbon source for high-density fermentation of Bifidobacterium,yielding a low-cost,highly biocompatible Ulva lactuca bifidobacterium ferment filtrate(ULBFF).Its peptide profile and molecular weight distribution were characterized by LC/MS.Cosmetic potential was systematically evaluated through stability testing,HET-CAM test,ABTS+∙/DPPH∙scavenging,NO inhibition,melanin suppression,and type Ⅰ collagen promotion.The results indicated a rich distribution of peptides,predominantly low-molecular-weight oligopeptides,with 92.92%of peptides containing≤20 amino acids and 84.89%of components having a molecular weight<500 Da.Formulations exhibited excellent stability and low irritation.Furthermore,they showed significantly enhanced efficacy in key areas,including antioxidant and antiinflammatory activity,melanin suppression,and collagen promotion.The ULBFF improved multifunctional performance while maintaining formulation stability and safety,demonstrating high scalability and potential for marine bioresource utilization in functional cosmetics.
基金funded by Beijing Natural Science Foundation(6252001)Guangdong Basic and Applied Basic Research Foundation(2022A1515140021)Natural Science Foundation of China(31871772).
文摘Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a natural physical barrier against external interference.Our previous study established a dynamic culture system of the biofilm-state Bifidobacterium adolescentis Gr19(B-DC-B.adolescentis Gr19),forming higher density and more structurally stable biofilms,which enhanced its potential probiotic properties in vivo.Thus,the protective effect and mechanism of B-DC-B.adolescentis Gr19 on lipopolysaccharide(LPS)-induced intestinal barrier dysfunction were investigated in this study.The results showed that B-DC-B.adolescentis Gr19 not only had high resistance and adhesion activity,but also improved the intestinal barrier by increasing goblet cells and promoting the expression of tight junction(TJ)-related proteins.Moreover,B-DC-B.adolescentis Gr19 effectively attenuated intestinal barrier injury in Caco-2 cells by improving intestinal permeability and integrity.Remarkably,B-DC-B.adolescentis Gr19 enhanced expression of TJ proteins,restored localization of cytoskeleton and reduced intestinal inflammation by suppressing the Ras homolog family member A/Rho-associated coiled-coil-forming kinases/nuclear factor kappa B/myosin light chain kinase/myosin light chain(RhoA/ROCK/NF-κB/MLCK/MLC)pathway.Therefore,B-DC-B.adolescentis Gr19 plays a key role in mitigating LPS-induced intestinal barrier dysfunction.Overall,the present study provides a theoretical basis for ameliorating intestinal barrier dysfunction and developing novel functional foods by using biofilm-state probiotics under dynamic culture.
基金supported by the National Natural Science Foundation of China(32021005)111 project(BP0719028)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘This study aimed to investigate the effects of infant feces-derived Bifidobacterium breve CCFM1078 on rheumatoid cachexia(RC).Twenty-four female Wistar rats were assigned to 3 groups:CON group(normal saline by gavage),CIA group(collagen-induced arthritis(CIA),normal saline by gavage),and CCFM1078 group(CIA,3×10^(9)CFU/(rat·day)B.breve CCFM1078 gavage).The results demonstrated that B.breve CCFM1078 not only improved skeletal muscle function in CIA rats,but also modulated the gut microbiota,skeletal muscle metabolism and hormone levels,reduced inflammation in the knee joint and skeletal muscles,decreased activity of the nuclear factor κB(NF-κB)inflammatory signaling pathway,enhanced the insulin receptor substrate 1(IRS1)/phosphatidylinositol 3-kinase/protein kinase(PI3K/Akt)signaling pathway,promoted skeletal muscle differentiation,and maintained skeletal muscle fiber diameter,consequently slowing down the progression of RC.These findings suggested that B.breve CCFM1078 may have a beneficial role as part of a dietary intervention for RC,enhancing overall therapeutic effects.
文摘脂代谢紊乱是肥胖及代谢综合征的核心诱因,利用益生菌调控肠道菌群已成为营养干预的重要策略。本研究以斑马鱼为模型,评价了Bifidobacterium breve 207-1(B.breve 207-1)及其灭活形态对脂代谢的调节作用。结果表明,二者均能通过调控ucp1等关键基因,促进脂肪分解与吸收。在降血脂方面,活菌特异性降低胆固醇,而灭活菌能同时降低胆固醇与甘油三酯,展现出更广谱的稳定功效,为后生元功能食品开发提供了新思路。
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
基金supported by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration saline)and the CCFM1078 group(administration B.breve CCFM1078,3×10^(9) CFU/day)in 3-,4-,and 5-week tests.All the groups have male and female distinctions.Our findings demonstrate that B.breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites(hexadecanamide,linoleoyl ethanolamide,and palmitoyl ethanolamide),the genes and proteins expression related to the growth hormone(GH)/insulin-like growth factors-1(IGF-1)axis and Gs/PKA/CREB signaling pathways,as well as downstream osteogenic and osteoclastic differentiation factors.The effects of B.breve CCFM1078 were different with age and gender dependent.This finding suggests B.breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.
基金supported by grants from the National Key Research and Development Program of China(2022YFA1303900 and 2021YFC2700705)the National Natural Science Foundation of China(82103919).
文摘The impact of mother-infant microbiota on neurodevelopment is an area of interest,but longitudinal studies are scarce.Using a cohort of 520 families from the Jiangsu birth cohort in China,we reveal that the maternal gut microbiota during early pregnancy play a substantial role,accounting for 3.34%of the variance in offspring neurodevelopmental scores.This contribution is notably higher than the 1.24%attributed to the infants’own microbiota at 1 year of age,underscoring the significant influence of maternal gut health on early child development.Remarkably,an elevation in maternal Bifidobacterium pseudocatenulatum is linked to decreased cognitive scores,whereas an enrichment of Bifidobacterium longum at 1 year of age is associated with higher cognitive scores.Furthermore,we find that maternal B.pseudocatenulatum is linked to the heterolactic fermentation metabolic pathway,while infant B.longum is associated with the Bifidobacterium shunt pathway.In summary,our analysis implies that maternal and infant gut microbiota play a distinct role in neurodevelopment,suggesting potential strategies for improving neurodevelopmental outcomes during early pregnancy or infant development by targeting gut microbiota composition.
文摘This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mirtazapine in treating postoperative depression of gastric cancer patients,also explored the multi-target therapeutic mechanisms of Bifidobacterium triple viable bacteria combined with mirtazapine in managing depression in patients after radical gastrectomy for gastric cancer for the first time.The results indicated that Bifidobacterium could alleviate depression in patients after radical gastrectomy for gastric cancer.Gastric cancer patients undergoing surgery frequently experience postoperative psychological disorders,notably depression.These conditions not only impair quality of life but also negatively impact disease-free and overall survival.This editorial explores the underestimated psychological challenges following gastric cancer surgery,including depression,anxiety,adjustment disorders,and post-traumatic stress disorder.It further examines the therapeutic potential of Bifidobacterium triple viable probiotics,combined with mirtazapine,in alleviating postoperative depression through microbiological,immunological,and neurological mechanisms.Clinical evidence highlights additional benefits,such as improved gastrointestinal function,enhanced immunity,and better nutritional status,underscoring a holistic approach to postoperative care.While promising,further large-scale trials are needed to optimize and personalize probiotic-based interventions.
基金supported by the National Natural Science Foundation of China(32272332,32021005,31820103010)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP22006,JUSRP51501)the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to alleviate obesity by modulating the microbiota.In this study,we found that oral administration of Bifidobacterium adolescentis CCFM8630 to obese mice inhibited high-fat diet(HFD)-induced changes in body weight and adipose tissue and alleviated hepatic oxidative stress.Furthermore,B.adolescentis CCFM8630 treatment primarily affected the relative abundances of the phyla Proteobacteria and Actinobacteria,and thereby decreased the production of lipopolysaccharide(LPS)and the occurrence of LPS related diseases.A high fiber intake increased the abundance of Lactobacillus and the concentrations of short chain fatty acids in obese mice,but these changes were reversed by B.adolescentis CCFM8630 treatment.In addition,targeted metabolomic analysis and microbiota relationship analysis revealed that B.adolescentis CCFM8630 treatment modified the microbiota of obese mice by promoting the conversion of tryptophan(Trp)to xanthurenic acid,kynurenic acid,tryptamine,indole-3-acetic acid,and indole-3-carboxaldehyde;facilitated the expression of interleukin-17A and the aryl hydrocarbon receptor to generate interleukin-22 in the colon;and upregulated the expression of tight junction proteins,thereby strengthening intestinal barriers.In summary,our findings suggest that the intake of B.adolescentis CCFM8630 may alleviate obesity by modulating the gut microbiota and related Trp metabolism.
文摘BACKGROUND Patients with gastric cancer may have a risk of depression after radical surgery,which affects recovery and requires intensive intervention.AIM To explore the role of Bifidobacterium triple viable bacteria-assisted mirtazapine in patients with depression after radical surgery for gastric cancer.METHODS This study included 80 patients with gastric cancer who experienced depression after undergoing radical surgery from January 2022 to December 2023 at Jiangnan University Hospital in Wuxi city in Jiangsu province.The patients were categorized into the control group(n=40)treated with mirtazapine and observation group(n=40)treated with combined Bifidobacterium triple viable bacteria-assisted mirtazapine.Neuroendocrine index,intestinal flora level,nutrition status,and patient quality of life were analyzed.RESULTS Before drug index level,no statistically significant difference was observed between the two groups (P>0.05).After drug administration,both groups experienced a decline in depression scores,cortisol,Escherichia coli,Enterococcus faecalis levels,and quality of life scores,whereas dopamine,serotonin,Lactobacillus,Bifidobacteria,hemoglobin,serum albumin and prealbumin levels increased.The observation group demonstrated significantly greater improvements across these indicators,with significant differences within groups and between groups(P<0.05).CONCLUSION Bifidobacterium has a relieving effect on depression in patients after radical surgery for gastric cancer,improves neuroendocrine status,regulates intestinal flora,and improves nutritional status and quality of life.
基金supported by grants from the National Natural Science Foundation of China(32125031)the Fundamental Research Funds for the Central Universities(JUSRP222001)Collaborative Innovation Center for Food Safety and Quality Control,China。
文摘Aflatoxin B_1(AFB_1)is a common contaminant in cereals of global concern,and long-term low-dose exposure can adversely affect human health.Here,we showed that populations with dietary patterns characterized by high-fat diet(HFD)might have an increased risk of exposure to high levels of AFB_1.Our data indicated that chronic exposure of AFB_1 induced“gut-liver axis”injury in mice under HFD and normal diet(ND)patterns.AFB_1 further aggravated hepatic and intestinal injury,and intestinal microbiota disruption in HFD mice.Bifidobacterium breve BAA-2849 intervention analysis showed that liver injury and lipid disorders caused by AFB_1 exposure were alleviated by regulating the proportions of different gut microbes.We demonstrated through a mice model that the populations with a dietary pattern of HFD might be more susceptible to AFB_1 exposure and adverse effects on the gut-liver axis,and the toxicity of AFB_1 exposure can be alleviated by regulating the gut microbiota.
基金Project Name:Correlation Analysis between Intestinal Flora and Differential Proteins in Intestinal Tissue of Neonatal Necrotizing Enterocolitis(Project No.:FYX202336)。
文摘Objective:To analyze the efficacy of Bifidobacterium triple viable bacteria tablets on neonatal necrotizing enterocolitis(NEC)and its impact on serum factors of the patients.Methods:From January 2021 to May 2025,88 neonates with NEC admitted to our hospital were selected as study subjects.During the study,these 88 patients were evenly divided into two groups,namely the observation group and the control group,with 44 patients in each group based on the random number table method.In terms of treatment,the control group was treated with meropenem,while the observation group received additional treatment with Bifidobacterium triple viable bacteria powder based on the treatment plan of the control group.The clinical efficacy and differences in serum inflammatory factor levels between the two groups were compared.Results:The efficacy of the observation group(90.91%)was better than that of the control group(72.73%)(P<0.05).After treatment,the levels of C-reactive protein(CRP)and procalcitonin(PCT)in both groups decreased compared to those before treatment,and the values of the above indicators in the observation group were lower than those in the control group(P<0.05).Conclusion:Based on conventional treatment for NEC neonates,the use of Bifidobacterium triple viable bacteria tablets has significant efficacy and can effectively reduce serum inflammatory factor levels.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
文摘Objective： To construct Bifidobacterium Infantis/CD targeting gene therapy system. Methods： CD gene was amplified from E. Coli K12λ using PCR method, pGEX-1LamdaT plasmid and CD gene were digested with dual restriction endonucleas of EcoR Ⅰ and BamH Ⅰ and two segments of 4.9 kb and 1.3 kb were obtained. T4 DNA ligase was added to these two segments to make a recombinant CD/pGEX-1LamdaT plasmid. Then the recombinant plasmid was transfected into Bifidobacterium Infantis by electroporation. The recombinant plasmid was extracted from the positively transfected Bifidobacterium Infantis and digested with dual restriction endonucleases. Then the size of digested fragments was detected and sequencing of the gene segment inserted in extracted recombinant plasmid was performed according to the method of Sanger dideoxynucleotide triphosphate chain termination. Results： 6.2 kb recombinant plasmid was obtained from the positively transfected bacterial colony of Bifidobacterium Infantis. After being digested with dual restriction endonucleases, two segments of approximate 4.9 kb and 1.3 kb were gained from the extracted recombinant plasmid, which were equal to the size of pGEX-1LamdaT plasmid and CD gene, respectively. The full length and sequence of nucleotide acid of the inserted gene in extracted recombinant plasmid was completely identical to the CD gene. Conclusion： The foreign gene, CD gene was correctly inserted into pGEX-1LambdaT plasmid and transferred into Bifidobacterium Infantis. Bifidobacterium Infantis/CD targeting gene therapy system was successfully constructed.
