Objective:To evaluate in vitro antioxidant and antibacterial activity of methanolic extract of Arnebia benthamii（A.benthamii） whole plant.Methods:Plasmid damage was analyzed by agarose gell electrophoresis.Calf thym...Objective:To evaluate in vitro antioxidant and antibacterial activity of methanolic extract of Arnebia benthamii（A.benthamii） whole plant.Methods:Plasmid damage was analyzed by agarose gell electrophoresis.Calf thymus DNA was monitored by TBARS formation.DPPH, reducing power and lipid peroxidation was evaluated by using standard procedures.Antibacterial assay was monitored by disc diffusion method.Results:DPPH radical scavenging and hydroxyl radical scavenging potential of the plant revealed that the extract to be active radical scavenger.Reducing(Fe<sup>3+</sup>-Fe<sup>2+</sup>) power and lipid peroxidation inhibition efficiency（TBARS assay） of the extract was also evaluated and the extract showed promising activity in preventing lipid peroxidation and might prevent oxidative damages to biomolecules.The extract offered a significant protection against plasmid and calf thymus UNA damage induced by hydroxyl radicals. The extract was also evaluated on different bacterial strains and the maximum antibacterial activity was exhibited against Escherichia coli（E.coli） when compared with standard drug. Conclusions:These findings demonstrate that the methanol extract of A.benthamii has excellent anti-oxidant activities and could be considered as a potential source of lead molecules for pharmaceutical industries.展开更多
A research plot of Eucalyptus benthamii was planted to evaluate this species’ ability to supply the emerging bioenergy markets that are developing in the southern U.S. The plot was planted in two different densities ...A research plot of Eucalyptus benthamii was planted to evaluate this species’ ability to supply the emerging bioenergy markets that are developing in the southern U.S. The plot was planted in two different densities to investigate the growth parameters and the cold tolerance. The stand was measured annually through five growing seasons. The results indicated that the growth difference among the young E. benthamii was noticeable. For example, the maximum and minimum value of five-year old trees at diameter breast height (DBH) was 27.9 centimeters and 1.27 centimeters;and the maximum and minimum value of tree height was 22.86 meters and 2.44 meters, respectively. The yearly change in DBH and height of E. benthamii had significant differences. The average annual survival rates of E. benthamii had differences under the two planting densities (1650 trees ha-1 and 1237 trees ha-1). The densities also had effects on the height and DBH growth of E. benthamii. The average DBH and height of 1650 trees ha-1 plantation were 11.18 centimeters and 15.03 meters, and the average DBH and height of 1237 trees ha-1 plantation were 13.46 centimeters and 16.28 meters. The volume per hectare of 1650 trees ha-1 and 1237 trees ha-1 plantation were 111.45 cubic meters and 101.15 cubic meters, respectively. Average diameter growth was almost 2.54 centimeters per year and average height growth was over 3 meters. E. benthamii plantations were considered tolerant to -7.4 degrees Celsius and a cold spell during early 2014 (-11.3 degrees Celsius for two consecutive nights) killed the plantation. The growth of E. benthamii also varied depending on surrounding conditions. The difference in growth of row seven versus row one was a good example. The reason probably was that row seven was adjacent to a loblolly pine plantation and row one was next to an open field.展开更多
The present study was designed to investigate the chemical constituents of the whole herb of Dichrocephala benthamii. A new megastigmane glucoside(compound 1), together with its four known analogues(compounds 2-5), wa...The present study was designed to investigate the chemical constituents of the whole herb of Dichrocephala benthamii. A new megastigmane glucoside(compound 1), together with its four known analogues(compounds 2-5), was obtained. Their structures were elucidated on the basis of spectroscopic analyses(UV, IR, MS, and 1D and 2D NMR). The absolute configuration of compound 1was assigned on the basis of CD method and chemical evidence. In addition, their cytotoxicity against human hepatoma cells(Hep G-2) was evaluated by the MTT method. Compound 5 showed weak activity against Hep G-2, while the other compounds did not show remarkable inhibitory effects.展开更多
<span style="font-family:""><span style="font-family:Verdana;">The mixture of </span><i><span style="font-family:Verdana;">Erigeron floribundus</span...<span style="font-family:""><span style="font-family:Verdana;">The mixture of </span><i><span style="font-family:Verdana;">Erigeron floribundus</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Tragia benthamii</span></i><span style="font-family:Verdana;"> (AEMEFTB) is</span></span><span style="font-family:""> <span style="font-family:Verdana;">tra</span><span style="font-family:Verdana;">ditionally used against pelvic pain, dysmenorrhea and female sexual dysfunctions. In a recent study, we showed that the aqueous extract of the mixture of </span><span style="font-family:Verdana;">AEMEFTB suppresses the endometrium growth in rat</span></span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> with experimental</span><span style="font-family:""> </span><span style="font-family:Verdana;">en</span><span style="font-family:Verdana;">dometriosis. </span><span style="font-family:Verdana;">The </span><span style="font-family:""><span style="font-family:Verdana;">present study was aimed at investigating the effects of AEM</span><span style="font-family:Verdana;">EFTB on estrogen’s sensitive sexual organs growth and architecture. Imma</span><span style="font-family:Verdana;">ture gonado-intact female rats were randomly distributed into 7 groups of 5 ani</span><span style="font-family:Verdana;">mals each and daily treated during one week with either distilled water (10</span><span> </span><span style="font-family:Verdana;">ml/kg), refined palm oil (1</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">ml/kg) or 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (1</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">μg/day). Plant extract</span><span style="font-family:""><span style="font-family:Verdana;"> groups received aqueous extract of AEMEFTB at 130 or 260 mg/kg. The remaining groups were co-administered with 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (1</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">μg) plus 130 or 260 mg/kg of the plant mixture. Moreover, thirty-five immature female rats were bilaterally ovariectomized, then left and treated as before. Five other females, </span><span style="font-family:Verdana;">considered as sham animals, orally received distilled water (10 ml/kg). The</span><span style="font-family:Verdana;"> body weight of each animal was recorded daily and at the end of the treatment (day 8), animals were</span><span style="font-family:Verdana;"> sacrificed under anesthesia</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">vagina</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;">, uterus</span><span style="font-family:Verdana;">es</span><span style="font-family:""> <span style="font-family:Verdana;">and ovaries (if any) </span><span style="font-family:Verdana;">were collected for analysis. Treatment with AEMEFTB did not affect the ova</span><span style="font-family:Verdana;">rian </span><span style="font-family:Verdana;">weight and architecture in gonado-intact immature female rats. However, a</span><span style="font-family:Verdana;"> moderate increase of the uterine weight was recorded in animals treated with plant </span><span style="font-family:Verdana;">mixture at </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">high dose (260 mg/kg). On the contrary, a drop in the uterine</span><span style="font-family:""> </span><span style="font-family:Verdana;">growth index and total plasmatic proteins w</span><span style="font-family:Verdana;">as </span><span style="font-family:Verdana;">observed in immature females </span><span style="font-family:Verdana;">co-administered with the extract and estradiol. Results from this work showed</span><span style="font-family:""><span style="font-family:Verdana;"> that the mixture of </span><i><span style="font-family:Verdana;">Erigeron flori</span><span style="font-family:Verdana;">bundus</span></i><span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Tragia benthamii </span></i><span style="font-family:Verdana;">possesses a weak but observable estro</span></span><span style="font-family:Verdana;">gen-mimetic potential.展开更多
基金funded by National Medicinal Plants Board,Department of AYUSH,Ministry of Health and Family Welfare,GOI,to Dr.M.A Zargar wide grant No. Z18017-187/PR/GO/JK/04/2005-06/NMPB
文摘Objective:To evaluate in vitro antioxidant and antibacterial activity of methanolic extract of Arnebia benthamii（A.benthamii） whole plant.Methods:Plasmid damage was analyzed by agarose gell electrophoresis.Calf thymus DNA was monitored by TBARS formation.DPPH, reducing power and lipid peroxidation was evaluated by using standard procedures.Antibacterial assay was monitored by disc diffusion method.Results:DPPH radical scavenging and hydroxyl radical scavenging potential of the plant revealed that the extract to be active radical scavenger.Reducing(Fe<sup>3+</sup>-Fe<sup>2+</sup>) power and lipid peroxidation inhibition efficiency（TBARS assay） of the extract was also evaluated and the extract showed promising activity in preventing lipid peroxidation and might prevent oxidative damages to biomolecules.The extract offered a significant protection against plasmid and calf thymus UNA damage induced by hydroxyl radicals. The extract was also evaluated on different bacterial strains and the maximum antibacterial activity was exhibited against Escherichia coli（E.coli） when compared with standard drug. Conclusions:These findings demonstrate that the methanol extract of A.benthamii has excellent anti-oxidant activities and could be considered as a potential source of lead molecules for pharmaceutical industries.
文摘A research plot of Eucalyptus benthamii was planted to evaluate this species’ ability to supply the emerging bioenergy markets that are developing in the southern U.S. The plot was planted in two different densities to investigate the growth parameters and the cold tolerance. The stand was measured annually through five growing seasons. The results indicated that the growth difference among the young E. benthamii was noticeable. For example, the maximum and minimum value of five-year old trees at diameter breast height (DBH) was 27.9 centimeters and 1.27 centimeters;and the maximum and minimum value of tree height was 22.86 meters and 2.44 meters, respectively. The yearly change in DBH and height of E. benthamii had significant differences. The average annual survival rates of E. benthamii had differences under the two planting densities (1650 trees ha-1 and 1237 trees ha-1). The densities also had effects on the height and DBH growth of E. benthamii. The average DBH and height of 1650 trees ha-1 plantation were 11.18 centimeters and 15.03 meters, and the average DBH and height of 1237 trees ha-1 plantation were 13.46 centimeters and 16.28 meters. The volume per hectare of 1650 trees ha-1 and 1237 trees ha-1 plantation were 111.45 cubic meters and 101.15 cubic meters, respectively. Average diameter growth was almost 2.54 centimeters per year and average height growth was over 3 meters. E. benthamii plantations were considered tolerant to -7.4 degrees Celsius and a cold spell during early 2014 (-11.3 degrees Celsius for two consecutive nights) killed the plantation. The growth of E. benthamii also varied depending on surrounding conditions. The difference in growth of row seven versus row one was a good example. The reason probably was that row seven was adjacent to a loblolly pine plantation and row one was next to an open field.
基金supported financially by the Chinese National S&T Special Project on Major New Drug Innovation(No.2013ZX09508104)Innovative Research Team in Institute of Medicinal Plant Development(IMPLD)
文摘The present study was designed to investigate the chemical constituents of the whole herb of Dichrocephala benthamii. A new megastigmane glucoside(compound 1), together with its four known analogues(compounds 2-5), was obtained. Their structures were elucidated on the basis of spectroscopic analyses(UV, IR, MS, and 1D and 2D NMR). The absolute configuration of compound 1was assigned on the basis of CD method and chemical evidence. In addition, their cytotoxicity against human hepatoma cells(Hep G-2) was evaluated by the MTT method. Compound 5 showed weak activity against Hep G-2, while the other compounds did not show remarkable inhibitory effects.
文摘<span style="font-family:""><span style="font-family:Verdana;">The mixture of </span><i><span style="font-family:Verdana;">Erigeron floribundus</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Tragia benthamii</span></i><span style="font-family:Verdana;"> (AEMEFTB) is</span></span><span style="font-family:""> <span style="font-family:Verdana;">tra</span><span style="font-family:Verdana;">ditionally used against pelvic pain, dysmenorrhea and female sexual dysfunctions. In a recent study, we showed that the aqueous extract of the mixture of </span><span style="font-family:Verdana;">AEMEFTB suppresses the endometrium growth in rat</span></span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> with experimental</span><span style="font-family:""> </span><span style="font-family:Verdana;">en</span><span style="font-family:Verdana;">dometriosis. </span><span style="font-family:Verdana;">The </span><span style="font-family:""><span style="font-family:Verdana;">present study was aimed at investigating the effects of AEM</span><span style="font-family:Verdana;">EFTB on estrogen’s sensitive sexual organs growth and architecture. Imma</span><span style="font-family:Verdana;">ture gonado-intact female rats were randomly distributed into 7 groups of 5 ani</span><span style="font-family:Verdana;">mals each and daily treated during one week with either distilled water (10</span><span> </span><span style="font-family:Verdana;">ml/kg), refined palm oil (1</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">ml/kg) or 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (1</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">μg/day). Plant extract</span><span style="font-family:""><span style="font-family:Verdana;"> groups received aqueous extract of AEMEFTB at 130 or 260 mg/kg. The remaining groups were co-administered with 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (1</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">μg) plus 130 or 260 mg/kg of the plant mixture. Moreover, thirty-five immature female rats were bilaterally ovariectomized, then left and treated as before. Five other females, </span><span style="font-family:Verdana;">considered as sham animals, orally received distilled water (10 ml/kg). The</span><span style="font-family:Verdana;"> body weight of each animal was recorded daily and at the end of the treatment (day 8), animals were</span><span style="font-family:Verdana;"> sacrificed under anesthesia</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">vagina</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;">, uterus</span><span style="font-family:Verdana;">es</span><span style="font-family:""> <span style="font-family:Verdana;">and ovaries (if any) </span><span style="font-family:Verdana;">were collected for analysis. Treatment with AEMEFTB did not affect the ova</span><span style="font-family:Verdana;">rian </span><span style="font-family:Verdana;">weight and architecture in gonado-intact immature female rats. However, a</span><span style="font-family:Verdana;"> moderate increase of the uterine weight was recorded in animals treated with plant </span><span style="font-family:Verdana;">mixture at </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">high dose (260 mg/kg). On the contrary, a drop in the uterine</span><span style="font-family:""> </span><span style="font-family:Verdana;">growth index and total plasmatic proteins w</span><span style="font-family:Verdana;">as </span><span style="font-family:Verdana;">observed in immature females </span><span style="font-family:Verdana;">co-administered with the extract and estradiol. Results from this work showed</span><span style="font-family:""><span style="font-family:Verdana;"> that the mixture of </span><i><span style="font-family:Verdana;">Erigeron flori</span><span style="font-family:Verdana;">bundus</span></i><span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Tragia benthamii </span></i><span style="font-family:Verdana;">possesses a weak but observable estro</span></span><span style="font-family:Verdana;">gen-mimetic potential.
