The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples...The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples of five different geographic origins: Eastern China.Central China.South-western China,North-western China and North-eastern China.Principal component analysis and SIMCA are applied to effectively classifying the samples according to the origin of the plants.The chemical information contained in the high resolution gas chromatographic data is sufficient to characterize the geographic origin of sam- pies.展开更多
OBJECTIVE:To identify the antitumor effects of Gualou Beimu Yin(瓜蒌贝母饮,GLBMY)in triple-negative breast cancer(TNBC)and to explore the underlying mechanisms.METHODS:A mouse model of breast cancer was established an...OBJECTIVE:To identify the antitumor effects of Gualou Beimu Yin(瓜蒌贝母饮,GLBMY)in triple-negative breast cancer(TNBC)and to explore the underlying mechanisms.METHODS:A mouse model of breast cancer was established and treated with GLBMY.Freeze-dried GLBMY powder was used to treat MDA-MB-231 and BT549 cells to assess the therapeutic efficacy of GLBMY against TNBC.Network pharmacology,transcriptomics and metabolomics were employed to identify the potential mechanism of GLBMY in TNBC treatment.Finally,the main regulating genes and proteins in the enriched pathways were validated by Quantitative real-time polymerase chain reaction(q PCR)and Western blotting analysis to confirm its mechanism.RESULTS:GLBMY can inhibit the growth of TNBC through apoptosis and necroptosis pathways and inhibit TNBC lung metastasis by inhibiting epithelialmesenchymal transition(EMT).Network pharmacology has elucidated the most important active ingredients(tubeimoside I,emodin,cucurbitacin,and ursolic acid)and the most critical targets[interleukin-6(IL6),signal transducer and activator of transcription 3(STAT3),mitogen-activated protein kinase 3(MAPK3)]of GLBMY in treating TNBC.RNA-seq revealed that GLBMY affected the nuclear factor kappa-light-chain-enhancer of activated B cells,rat sarcoma virus,and MAPK signalling pathways.Metabolomics revealed that the metabolites mainly affected by GLBMY were L-(+)-lactic acid,isocitric acid,benzoic acid and indoxyl sulfate.Subsequent experiments demonstrated that GLBMY can inhibit EMT in TNBC through the MAPK/ERK pathway and inhibit the proliferation and progression of TNBC through the IL6-STAT signalling pathway.CONCLUSIONS:We confirmed that GLBMY inhibits the development and metastasis of TNBC through the MAPK/Erk and IL6-STAT signalling pathways.GLBMY shows promise as a long-term supplementary or alternative therapy for TNBC,offering new insights for TNBC treatment.展开更多
AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagen...AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.展开更多
文摘The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples of five different geographic origins: Eastern China.Central China.South-western China,North-western China and North-eastern China.Principal component analysis and SIMCA are applied to effectively classifying the samples according to the origin of the plants.The chemical information contained in the high resolution gas chromatographic data is sufficient to characterize the geographic origin of sam- pies.
基金Supported by the National Natural Science Foundation of China:Study on Mechanism of Transdifferentiation of Hepatocellular Carcinoma Cells Induced by Huoxue Ruanjian Traditional Chinese Medicine (No. 82174103)。
文摘OBJECTIVE:To identify the antitumor effects of Gualou Beimu Yin(瓜蒌贝母饮,GLBMY)in triple-negative breast cancer(TNBC)and to explore the underlying mechanisms.METHODS:A mouse model of breast cancer was established and treated with GLBMY.Freeze-dried GLBMY powder was used to treat MDA-MB-231 and BT549 cells to assess the therapeutic efficacy of GLBMY against TNBC.Network pharmacology,transcriptomics and metabolomics were employed to identify the potential mechanism of GLBMY in TNBC treatment.Finally,the main regulating genes and proteins in the enriched pathways were validated by Quantitative real-time polymerase chain reaction(q PCR)and Western blotting analysis to confirm its mechanism.RESULTS:GLBMY can inhibit the growth of TNBC through apoptosis and necroptosis pathways and inhibit TNBC lung metastasis by inhibiting epithelialmesenchymal transition(EMT).Network pharmacology has elucidated the most important active ingredients(tubeimoside I,emodin,cucurbitacin,and ursolic acid)and the most critical targets[interleukin-6(IL6),signal transducer and activator of transcription 3(STAT3),mitogen-activated protein kinase 3(MAPK3)]of GLBMY in treating TNBC.RNA-seq revealed that GLBMY affected the nuclear factor kappa-light-chain-enhancer of activated B cells,rat sarcoma virus,and MAPK signalling pathways.Metabolomics revealed that the metabolites mainly affected by GLBMY were L-(+)-lactic acid,isocitric acid,benzoic acid and indoxyl sulfate.Subsequent experiments demonstrated that GLBMY can inhibit EMT in TNBC through the MAPK/ERK pathway and inhibit the proliferation and progression of TNBC through the IL6-STAT signalling pathway.CONCLUSIONS:We confirmed that GLBMY inhibits the development and metastasis of TNBC through the MAPK/Erk and IL6-STAT signalling pathways.GLBMY shows promise as a long-term supplementary or alternative therapy for TNBC,offering new insights for TNBC treatment.
文摘AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.
