The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples...The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples of five different geographic origins: Eastern China.Central China.South-western China,North-western China and North-eastern China.Principal component analysis and SIMCA are applied to effectively classifying the samples according to the origin of the plants.The chemical information contained in the high resolution gas chromatographic data is sufficient to characterize the geographic origin of sam- pies.展开更多
AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagen...AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.展开更多
文摘The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples of five different geographic origins: Eastern China.Central China.South-western China,North-western China and North-eastern China.Principal component analysis and SIMCA are applied to effectively classifying the samples according to the origin of the plants.The chemical information contained in the high resolution gas chromatographic data is sufficient to characterize the geographic origin of sam- pies.
文摘AIM: In order to identify “Beimu” by molecular biology technology, the extraction method of genomic DNA and PCR amplification were studied. METHODS: The genomic DNA extraction methods by SDS, CTAB and DNA zol reagent were compared, so were DNAs from samples treated with different methods. The effects of DNA quality, annealing temperature and activity of Taq DNA polymerase on PCR products were studied also. RESULTS: The SDS method is simpler and cheaper. The fragment of genomic DNA extracted from fresh sample was longer and purer. The annealing temperature and activity of Taq DNA polymerase showed little effect on PCR amplification, while the quality of DNA affected the PCR amplification directly. CONCLUSION: By extracting genomic DNA from fresh sample and PCR amplification, a specific band was obtained.
