Experimental murine behaviors,including communication,emotional expression,social interaction,learning,and reproduction,play a critical role in various research fields such as genetics,neuroscience,and non-neuroscienc...Experimental murine behaviors,including communication,emotional expression,social interaction,learning,and reproduction,play a critical role in various research fields such as genetics,neuroscience,and non-neuroscience disciplines.The growing demand for murine behavior research has driven the development of diverse evaluation methods.Current evaluation techniques include assessments based on ordinary or high-speed cameras,active depth sensors,inertial sensors,optical pressure plates,electromyography,and two-photon fluorescence microscopy.Each method has its unique advantages and limitations depending on the experi-mental context.Therefore,selecting an appropriate evaluation method tailored to specific experimental re-quirements is crucial for obtaining accurate and meaningful results.This review summarizes the advancements in experimental murine behavior evaluation methods over the past decade,providing a comprehensive overview of their applications and contributions to behavioral research.展开更多
With the advantages of real-time analysis and visual evaluation results,intelligent technology-enabled teaching behavior evaluation has gradually become a powerful means to help teachers adjust teaching behaviors and ...With the advantages of real-time analysis and visual evaluation results,intelligent technology-enabled teaching behavior evaluation has gradually become a powerful means to help teachers adjust teaching behaviors and improve teaching quality.However,at present,the evaluation of intelligent teachers’behaviors is still in the preliminary exploration stage,and the application research is not deep enough.This paper analyzes the application of intelligent technology in the evaluation of teachers’classroom teaching behaviors from the perspectives of evaluation data,methods,and results.Voice print recognition technology is used to recognize the teachers’identities and track the speech in the classroom videos,and the videos are segmented.Then,the evaluation framework of teachers’classroom teaching behaviors is constructed using three dimensions of emotion,posture,and position preference.Finally,evaluation results are presented to teachers in a more intuitive and easy-to-understand visual way,to help teachers reflect on teaching.This paper aims to promote the transformation of teachers’classroom teaching behavior evaluation toward an intelligent,efficient,and sustainable direction through current research.展开更多
The development and supporting technologies of high-strain line pipe steel and pipes used for oil and gas pipeline projects in special geological environments(such as earthquake zone,landslide zone,mine goaf and subsi...The development and supporting technologies of high-strain line pipe steel and pipes used for oil and gas pipeline projects in special geological environments(such as earthquake zone,landslide zone,mine goaf and subsidence zone)is one of the international research hotspots,and it is also a major problem that China's major oil and gas pipeline projects have to solve.Focusing on a series of key technical difficulties in the research&development and application of high-strain line pipe steel and pipes,a number of theoretical and technological innovations have been achieved after more than ten years of joint researches.And the main achievements are as follows.First,the method of applying many different parameters(e.g.stress ratio,yield to tensile ratio and uniform elongation(UEL))to comprehensively characterize and evaluate the deformation behavior of steel pipes is proposed,and a technical index system and standard for the new products of X70HD/X80HD high-strain line pipe steel and pipes are established.Second,a complete set of X70HD/X80HD steel plate manufacturing technology is researched and developed,and the high-strain steel plate with the properties of low yield to tensile ratio,high uniform elongation,high stress ratio and high strength and toughness is worked out.Third,the X70HD/X80HD JCOE and UOE high-strain longitudinal submerged arc welded pipe manufacturing technology is researched and developed,reasonable performance matching between a steel pipe and a welding seam is realized,and the performance deterioration in the process of forming,welding,diameter-expending and thermal coating is solved.Fourth,a full-scale test device of steel pipe internal pressure t bending large deformation is independently developed,and the physical simulation based steel pipe deformation test technology is formed.X70HD/X80HD high-strain line pipe steel and pipes have been applied in the West to East Gas Pipeline,the ChinaeMyanmar Pipeline and other major gas pipeline projects in the large scale,and their application effects are remarkable.To satisfy the new needs of pipeline construction and long-term safe operation under complex geological conditions,some suggestions were put forwarded,such as developing new methods of strain-based pipeline design,and researching and developing or improving the supporting technologies suitable for the higher matching requirements of girth weld,such as welding methods,welding materials,welding processes,and girth weld performance quality and defect control requirements.展开更多
Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ische...Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.展开更多
基金supported by grants from:Beijing Natural Science Foundation 7232339(Ying Wang)Scientific Research Foundation of the National Health Commission/Zhejiang Provincial Major Science and Technology Plan Project of Medicine and Health(WKJ-ZJ-2412).
文摘Experimental murine behaviors,including communication,emotional expression,social interaction,learning,and reproduction,play a critical role in various research fields such as genetics,neuroscience,and non-neuroscience disciplines.The growing demand for murine behavior research has driven the development of diverse evaluation methods.Current evaluation techniques include assessments based on ordinary or high-speed cameras,active depth sensors,inertial sensors,optical pressure plates,electromyography,and two-photon fluorescence microscopy.Each method has its unique advantages and limitations depending on the experi-mental context.Therefore,selecting an appropriate evaluation method tailored to specific experimental re-quirements is crucial for obtaining accurate and meaningful results.This review summarizes the advancements in experimental murine behavior evaluation methods over the past decade,providing a comprehensive overview of their applications and contributions to behavioral research.
文摘With the advantages of real-time analysis and visual evaluation results,intelligent technology-enabled teaching behavior evaluation has gradually become a powerful means to help teachers adjust teaching behaviors and improve teaching quality.However,at present,the evaluation of intelligent teachers’behaviors is still in the preliminary exploration stage,and the application research is not deep enough.This paper analyzes the application of intelligent technology in the evaluation of teachers’classroom teaching behaviors from the perspectives of evaluation data,methods,and results.Voice print recognition technology is used to recognize the teachers’identities and track the speech in the classroom videos,and the videos are segmented.Then,the evaluation framework of teachers’classroom teaching behaviors is constructed using three dimensions of emotion,posture,and position preference.Finally,evaluation results are presented to teachers in a more intuitive and easy-to-understand visual way,to help teachers reflect on teaching.This paper aims to promote the transformation of teachers’classroom teaching behavior evaluation toward an intelligent,efficient,and sustainable direction through current research.
基金Project supported by the National Key R&D Program of China“Research and Development of High-strain Marine Line Pipes”(No.2018YFC03010300)PetroChina's Major Science&Technology Project“Research on Key Technologies for Controlling Pipe Fracture and Deformation in the Second West-East Gas Pipeline”(No.2009E-0105)+1 种基金“Research and Development of Special Welded Pipes for the Second West-East Gas Pipeline”(No.2009E-0104)CNPC's Science&Technology Infrastructure Program“CNPC Infrastructure Construction of Key Laboratory for Petroleum Tubular Engineering”(No.07H611).
文摘The development and supporting technologies of high-strain line pipe steel and pipes used for oil and gas pipeline projects in special geological environments(such as earthquake zone,landslide zone,mine goaf and subsidence zone)is one of the international research hotspots,and it is also a major problem that China's major oil and gas pipeline projects have to solve.Focusing on a series of key technical difficulties in the research&development and application of high-strain line pipe steel and pipes,a number of theoretical and technological innovations have been achieved after more than ten years of joint researches.And the main achievements are as follows.First,the method of applying many different parameters(e.g.stress ratio,yield to tensile ratio and uniform elongation(UEL))to comprehensively characterize and evaluate the deformation behavior of steel pipes is proposed,and a technical index system and standard for the new products of X70HD/X80HD high-strain line pipe steel and pipes are established.Second,a complete set of X70HD/X80HD steel plate manufacturing technology is researched and developed,and the high-strain steel plate with the properties of low yield to tensile ratio,high uniform elongation,high stress ratio and high strength and toughness is worked out.Third,the X70HD/X80HD JCOE and UOE high-strain longitudinal submerged arc welded pipe manufacturing technology is researched and developed,reasonable performance matching between a steel pipe and a welding seam is realized,and the performance deterioration in the process of forming,welding,diameter-expending and thermal coating is solved.Fourth,a full-scale test device of steel pipe internal pressure t bending large deformation is independently developed,and the physical simulation based steel pipe deformation test technology is formed.X70HD/X80HD high-strain line pipe steel and pipes have been applied in the West to East Gas Pipeline,the ChinaeMyanmar Pipeline and other major gas pipeline projects in the large scale,and their application effects are remarkable.To satisfy the new needs of pipeline construction and long-term safe operation under complex geological conditions,some suggestions were put forwarded,such as developing new methods of strain-based pipeline design,and researching and developing or improving the supporting technologies suitable for the higher matching requirements of girth weld,such as welding methods,welding materials,welding processes,and girth weld performance quality and defect control requirements.
基金supported by the National Natural Science Foundation of China,No.82001604Guizhou Provincial Higher Education Science and Technology Innovation Team,No.[2023]072+1 种基金Guizhou Province Distinguished Young Scientific and Technological Talent Program,No.YQK[2023]040Guizhou Provincial Basic Research Program(Natural Science),No.ZK[2021]-368(all to LXiong),and Zunyi City Innovative Talent Team Training Plan,No.[2022]-2.
文摘Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.
