Based on Larry A. Samovar’s theory and combined with my own teaching practice, this essay mainly analyzes four nonverbal codes: body behavior, silence, seating and personal space in teaching ESP students. Meanwhile, ...Based on Larry A. Samovar’s theory and combined with my own teaching practice, this essay mainly analyzes four nonverbal codes: body behavior, silence, seating and personal space in teaching ESP students. Meanwhile, it also provides suggestions on how to make full use of them so as to direct, stimulate and encourage students in classroom activities.展开更多
Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and tr...Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and treated with different concentrations(5-15 mg/ml) of the ethanol extract of Xanthii Fructus. The expression of mi R-21 and pi RNA55490 was analyzed by Realtime PCR. The proliferation capacity was detected by CCK-8 assay. The anchorage-independent growth capacity was measured by soft agar colony formation experiment. The cell invasion capacity was determined by Matrigel assay. Results: The Xanthii Fructus alcohol extract down-regulated the expression of the oncogenic snc RNAmi R-21, and up-regulated the expression of the tumor suppressive snc RNA pi RNA55490. At the functional level, proliferation, anchorage-independent growth, and invasion capacities of the lung cancer cells were strongly inhibited by the extract treatment. Conclusion: The Chinese herb Xanthii Fructus inhibits the malignant behaviors of lung cancer cells, and the inhibition is largely associated with its ability to change the expression of snc RNAs.展开更多
文摘Based on Larry A. Samovar’s theory and combined with my own teaching practice, this essay mainly analyzes four nonverbal codes: body behavior, silence, seating and personal space in teaching ESP students. Meanwhile, it also provides suggestions on how to make full use of them so as to direct, stimulate and encourage students in classroom activities.
文摘Objective: To investigate the influence of Xanthii Fructuson the expression of small non-coding RNAs(snc RNA) and the malignant behaviors of lung cancer cells. Method: A549 human lung cancer cells were cultured and treated with different concentrations(5-15 mg/ml) of the ethanol extract of Xanthii Fructus. The expression of mi R-21 and pi RNA55490 was analyzed by Realtime PCR. The proliferation capacity was detected by CCK-8 assay. The anchorage-independent growth capacity was measured by soft agar colony formation experiment. The cell invasion capacity was determined by Matrigel assay. Results: The Xanthii Fructus alcohol extract down-regulated the expression of the oncogenic snc RNAmi R-21, and up-regulated the expression of the tumor suppressive snc RNA pi RNA55490. At the functional level, proliferation, anchorage-independent growth, and invasion capacities of the lung cancer cells were strongly inhibited by the extract treatment. Conclusion: The Chinese herb Xanthii Fructus inhibits the malignant behaviors of lung cancer cells, and the inhibition is largely associated with its ability to change the expression of snc RNAs.
