The proposed work aims to study phenol components （flavonoids） of bee bread and pollen obtained in Georgia using the HPLC （high-performance liquid chromatography） method, and to determine the content of biological...The proposed work aims to study phenol components （flavonoids） of bee bread and pollen obtained in Georgia using the HPLC （high-performance liquid chromatography） method, and to determine the content of biologically active compounds in them. The samples were taken in lmereti region （west Georgia）. After extraction of samples, there were carried out spectral and HPLC analysis of compounds for the sake of qualitative and quantitative research of them. There are studied the flavonoid compounds of bee bread and pollen by using the HPLC methods, and naringin, rutin and quercetin are identified. Their amount counts about 20% of full content of flavonoids. The content of flavonols in bee bread and pollen has been established. It also has been determined that the amount of flavonoids during the storage period of products reduces to 6.17-5.03 g.kg-1.展开更多
Pharmacological, derivate and microbiological study of beebread based immunomodulatory drug was conducted. Iimmunomodulating properties of pellets ＂Apimun＂ in the form of antibodies formation in response to the EB a...Pharmacological, derivate and microbiological study of beebread based immunomodulatory drug was conducted. Iimmunomodulating properties of pellets ＂Apimun＂ in the form of antibodies formation in response to the EB antigen were studied. Clear immunostimulatory properties of pellets ＂Apimun＂ were reproduced in mature rats and mice with normal immune status. Pellets ＂Apimun＂ at doses 1.5 and 2.0 g/kg showed increased activity ofphagocytosis and processes of antibody formation inhibition of delayed hypersensitivity reactions. Antimicrobial activity of pellets ＂Apimun＂ was studied. It was proved that it did not have antimicrobial properties. Derivate studies were conducted and showed that the active substance and composition of the IMP was stable at normal temperature. The results of pharmacological, microbiological and derivate studies show the effectiveness of the pellets ＂Apimun＂ and prospects for further research in order to create a product for immune status improvement.展开更多
【目的】为发掘和利用蜂粮中拮抗菌资源,对分离获得的拮抗细菌菌株PC2进行分类鉴定,并测定其发酵液抑菌物质基本特性。【方法】采用改良牛津杯双层平板法测定菌株发酵液抑菌谱及温度、p H、紫外线和蛋白酶对其抑菌活性稳定性的影响,菌...【目的】为发掘和利用蜂粮中拮抗菌资源,对分离获得的拮抗细菌菌株PC2进行分类鉴定,并测定其发酵液抑菌物质基本特性。【方法】采用改良牛津杯双层平板法测定菌株发酵液抑菌谱及温度、p H、紫外线和蛋白酶对其抑菌活性稳定性的影响,菌株鉴定结合形态学、生理生化特征和16S r RNA基因序列分析,硫酸铵沉淀法和盐酸沉淀有机溶剂提取法进行抑菌活性物质的初步分离。【结果】从3种蜂粮中分离筛选得到17株拮抗菌株,其中1株细菌PC2以马铃薯葡萄糖液体培养基发酵制备的无菌发酵液对7种供试菌株具有较强抑制作用,经形态、生理生化特征及16S r RNA基因序列分析,将其初步鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens)。菌株发酵液抑菌活性对温度、酸和紫外线具有较强的稳定性,对蛋白酶K、胃蛋白酶、碱性蛋白酶处理敏感。菌株发酵液存在抑菌蛋白和脂肽类物质。【结论】菌株PC2在食品保鲜和农业生防中具有潜在的开发应用价值。展开更多
基金The authors express their gratitude to Shota Rustaveli National Science Foundation （Grant 11/16） for financial provision.
文摘The proposed work aims to study phenol components （flavonoids） of bee bread and pollen obtained in Georgia using the HPLC （high-performance liquid chromatography） method, and to determine the content of biologically active compounds in them. The samples were taken in lmereti region （west Georgia）. After extraction of samples, there were carried out spectral and HPLC analysis of compounds for the sake of qualitative and quantitative research of them. There are studied the flavonoid compounds of bee bread and pollen by using the HPLC methods, and naringin, rutin and quercetin are identified. Their amount counts about 20% of full content of flavonoids. The content of flavonols in bee bread and pollen has been established. It also has been determined that the amount of flavonoids during the storage period of products reduces to 6.17-5.03 g.kg-1.
文摘Pharmacological, derivate and microbiological study of beebread based immunomodulatory drug was conducted. Iimmunomodulating properties of pellets ＂Apimun＂ in the form of antibodies formation in response to the EB antigen were studied. Clear immunostimulatory properties of pellets ＂Apimun＂ were reproduced in mature rats and mice with normal immune status. Pellets ＂Apimun＂ at doses 1.5 and 2.0 g/kg showed increased activity ofphagocytosis and processes of antibody formation inhibition of delayed hypersensitivity reactions. Antimicrobial activity of pellets ＂Apimun＂ was studied. It was proved that it did not have antimicrobial properties. Derivate studies were conducted and showed that the active substance and composition of the IMP was stable at normal temperature. The results of pharmacological, microbiological and derivate studies show the effectiveness of the pellets ＂Apimun＂ and prospects for further research in order to create a product for immune status improvement.
文摘【目的】为发掘和利用蜂粮中拮抗菌资源,对分离获得的拮抗细菌菌株PC2进行分类鉴定,并测定其发酵液抑菌物质基本特性。【方法】采用改良牛津杯双层平板法测定菌株发酵液抑菌谱及温度、p H、紫外线和蛋白酶对其抑菌活性稳定性的影响,菌株鉴定结合形态学、生理生化特征和16S r RNA基因序列分析,硫酸铵沉淀法和盐酸沉淀有机溶剂提取法进行抑菌活性物质的初步分离。【结果】从3种蜂粮中分离筛选得到17株拮抗菌株,其中1株细菌PC2以马铃薯葡萄糖液体培养基发酵制备的无菌发酵液对7种供试菌株具有较强抑制作用,经形态、生理生化特征及16S r RNA基因序列分析,将其初步鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens)。菌株发酵液抑菌活性对温度、酸和紫外线具有较强的稳定性,对蛋白酶K、胃蛋白酶、碱性蛋白酶处理敏感。菌株发酵液存在抑菌蛋白和脂肽类物质。【结论】菌株PC2在食品保鲜和农业生防中具有潜在的开发应用价值。
