Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis. Yougui Pill（YGP）, a 10-component tradi...Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis. Yougui Pill（YGP）, a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells. In vivo experiments showed that YGP significantly reduced the number of apoptotic cells, down-regulated m RNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated m RNA expression of Bcl-2. These findings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.展开更多
目的通过研究重组人红细胞生成素（recombinant human erythropoietin，rhEPO）对局灶性脯缺血再灌注大鼠基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）和BCL-2表达的影响，探讨EPO脑保护作用的可能机制。方法应用线栓法建立...目的通过研究重组人红细胞生成素（recombinant human erythropoietin，rhEPO）对局灶性脯缺血再灌注大鼠基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）和BCL-2表达的影响，探讨EPO脑保护作用的可能机制。方法应用线栓法建立大鼠大脑中动脉闭塞再灌注（middle cerebral artery occlusion／reperfusion，MCAO／R）模型，腹腔注射rhEPO进行干预，通过HE染色光镜观察组织病理学变化，应用免疫组化技术检测缺血侧大脑皮质MMP-9和BCL-2的表达。结果HE染色：在各时间点，rhEPO组存活神经细胞数量显著较多，损伤程度显著较轻。MMP-9免疫组化染色：正常对照组和假手术组偶见阳性细胞；生理盐水对照组缺血侧脑组织MMP-9阳性细胞在再灌注后6h开始出现，24h达高峰，72h开始减少；rhEPO组MMP-9阳性细胞变化趋势与生理盐水对照组相似，但在同一时间点显著少于生理盐水对照组（t分别为12．0236、12．6350和12．7796，P均〈0．01）。BCL-2免疫组化染色：对照组和假手术组末见阳性细胞；牛理盐水对照组缺血侧脑组织BCL-2阳性细胞数在再灌注后6h达高峰，24h数量减少，72h进一步减少；rhEPO组BCL-2阳性细胞变化趋势与生理盐水对照组相似，但在同一时间点显著多于生理盐水对照组（t分别为5．7631、8．1101和5．7987，P均〈0．01）。结论rhEPO町能通过抑制MMP-9表达和上调BCL-2表达抑制缺血侧皮质神经细胞凋亡而发挥保护作用。展开更多
基金supported by the National Natural Science Foundation of China,No.30471576,and 30872277a grant from the Innovative Research Program in Universities of the Ministry of Education of China,No.IRT0760
文摘Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis. Yougui Pill（YGP）, a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells. In vivo experiments showed that YGP significantly reduced the number of apoptotic cells, down-regulated m RNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated m RNA expression of Bcl-2. These findings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.
