[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used t...[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used to evaluate the hair coloring effect and the absorbance of cleaning solution after hair was dyed. [Results] The optimum pet dog dyeing process of plant hair dye was dye concentration of50 mg/mL,dye pH of 7. 5 and dyeing time of 1 h. [Conclusions] The coloring and fixing effects of the dye and dyeing process are good,which can lay a foundation for the development and utilization of plant dyes.展开更多
Musa basjoo Sieb polysaccharide(MBSP)is the active ingredient of Musa basjoo Sieb.The purpose of this study was to preliminarily characterize the structural properties of MBSP,and to explore its ameliorating effect LP...Musa basjoo Sieb polysaccharide(MBSP)is the active ingredient of Musa basjoo Sieb.The purpose of this study was to preliminarily characterize the structural properties of MBSP,and to explore its ameliorating effect LPS-induced macrophage RAW264.7 inflammation and its therapeutic effect on DSS-induced zebrafish colitis.The data indicated that the average molecular weight of MBSP was 393.4 kDa,which was an acidic hetero-polysaccharide composed of seven monosaccharides.In the LPS-induced macrophage RAW264.7 inflammation model,we found that MBSP significantly reduced the level of inflammatory mediators(p<0.001).The release of NO,IL-6,and TNF-αwas reduced by 48.21%,65.45%,and 86.49%,respectively.And MBSP modulated intra-cellular ROS levels.This might be related to the activation of NF-κB/MAPK signaling pathway by MBSP.Additionally,MBSP significantly improved the oxidative stress status of UC zebrafish.SOD,CAT,GSH-Px were increased by 50.58%,39.07%and 65.86%,and MDA,MPO were decreased by 26.00%and 50.98%,respectively(p<0.001).Meanwhile,MBSP was also found to be effective in improving UC by activating the TLR4/NF-κB/MAPK signaling pathway in vivo.In conclusion,the present study characterized the structural properties of MBSP from various aspects,confirmed the anti-inflammatory effect of MBSP in vitro,and further verified the in vivo anti-inflammatory activity of MBSP using UC zebrafish as an animal model.The results of this study provide a reference for the development and application of MBSP in the field of treating inflammatory diseases.展开更多
基金Supported by the National Science and Technology Foundation Project of Traditional Chinese Veterinary Medicine Resources Rescuing and Organizing(2013FY110600-03)
文摘[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used to evaluate the hair coloring effect and the absorbance of cleaning solution after hair was dyed. [Results] The optimum pet dog dyeing process of plant hair dye was dye concentration of50 mg/mL,dye pH of 7. 5 and dyeing time of 1 h. [Conclusions] The coloring and fixing effects of the dye and dyeing process are good,which can lay a foundation for the development and utilization of plant dyes.
文摘Musa basjoo Sieb polysaccharide(MBSP)is the active ingredient of Musa basjoo Sieb.The purpose of this study was to preliminarily characterize the structural properties of MBSP,and to explore its ameliorating effect LPS-induced macrophage RAW264.7 inflammation and its therapeutic effect on DSS-induced zebrafish colitis.The data indicated that the average molecular weight of MBSP was 393.4 kDa,which was an acidic hetero-polysaccharide composed of seven monosaccharides.In the LPS-induced macrophage RAW264.7 inflammation model,we found that MBSP significantly reduced the level of inflammatory mediators(p<0.001).The release of NO,IL-6,and TNF-αwas reduced by 48.21%,65.45%,and 86.49%,respectively.And MBSP modulated intra-cellular ROS levels.This might be related to the activation of NF-κB/MAPK signaling pathway by MBSP.Additionally,MBSP significantly improved the oxidative stress status of UC zebrafish.SOD,CAT,GSH-Px were increased by 50.58%,39.07%and 65.86%,and MDA,MPO were decreased by 26.00%and 50.98%,respectively(p<0.001).Meanwhile,MBSP was also found to be effective in improving UC by activating the TLR4/NF-κB/MAPK signaling pathway in vivo.In conclusion,the present study characterized the structural properties of MBSP from various aspects,confirmed the anti-inflammatory effect of MBSP in vitro,and further verified the in vivo anti-inflammatory activity of MBSP using UC zebrafish as an animal model.The results of this study provide a reference for the development and application of MBSP in the field of treating inflammatory diseases.
基金This study was supported by the National Basic Research Program of China(973 Program,2007CB-411600)National Natural Science Foundation of China(No.30670160).
