Current trends in chiral analysis of pharmaceutical drugs are focused on faster separations and higher separation efficiencies, Core-shell or superficially porous particles （SPP） based chiral stationary phases （CSP...Current trends in chiral analysis of pharmaceutical drugs are focused on faster separations and higher separation efficiencies, Core-shell or superficially porous particles （SPP） based chiral stationary phases （CSPs） provide reduced analysis times while maintaining high column efficiencies and sensitivity. In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 μm SPP based vancomycin CSP （SPP-V） 10 cm ×0.21 cm column was compared to that of a corresponding 5 μm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2-5 time greater sensitivity and shorter analysis time for a set of 22 basic pharma- ceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racemic citalopram whose enantiomers could be selectively identified by MS.展开更多
Detection of drugs in hair has become popular in recent years. The significantly long drug detection window (months) in hair has allowed the retrospective investigation and measurement of past consumption of drug. As ...Detection of drugs in hair has become popular in recent years. The significantly long drug detection window (months) in hair has allowed the retrospective investigation and measurement of past consumption of drug. As the majority of drugs are basic, an extraction method was developed based on a methanolic solution for detection of basic/weak basic drugs in hair. It was compared with alkaline digestion (NaOH) followed by LLE. A filtration step with filtration vials was added and their materials were compared. After filtration, extracts were injected directly onto a C18 column coupled to Sciex ABI 2000 MSMS. The mobile phase was 50% methanol, 0.1% formic acid and 2 mM ammonium acetate (isocratic). Both methods were compared by applying them to real samples. Results showed that calibration was linear with r2 of 0.991-0.999 for 20 tested analytes. The matrix effect was assessed to be between 91.4%- 110.2% for 18 analytes. PTFE filter material showed better recoveries over the GMF and PVDF based filters. Stability of analytes during extraction in general was better with methanolic incubation rather than alkaline digestion. With regard to real sample recovery, 6 out of 10 analytes recovered better with alkaline digestion. In conclusion, the methanolic method is capable of extracting most basic drugs in hair samples but only part of the total incorporated drug. Therefore, these results suggest that a combination of both methods (methanolic and alkaline extractions) in hair sample processing for general detection of basic and weak basic drugs may produce better results. However, not all basic drugs are stable under alkaline digestion.展开更多
A novel pressurized capillary electrochromatography (PCEC) was developed to separate basic drugs on strong cation exchange (SCX) column. The separation result by using PCEC was better than that by using micro-HPLC. Th...A novel pressurized capillary electrochromatography (PCEC) was developed to separate basic drugs on strong cation exchange (SCX) column. The separation result by using PCEC was better than that by using micro-HPLC. The effects of electrical field and pressure on plate height and resolution were investigated. Influences of organic modifier, ionic strength and pH value of buffer on retention behavior were evaluated, and the separation mechanism was also discussed.展开更多
Basic research features its indelible contribution to and energetic promotion of scientific cause and advancing social progress. This article explores its vital position and rewarding role in creating socio-economic b...Basic research features its indelible contribution to and energetic promotion of scientific cause and advancing social progress. This article explores its vital position and rewarding role in creating socio-economic benefits by reviewing the development and marketing success of a new drug, "Di’ aoxinxuekang".展开更多
本文基于Web of Science、Medline、Nature Index、高被引科学家名单及世卫组织、FDA、NMPA、IQVIA等最新数据,系统分析全球医学科技战略演变、基础研究格局与医药研发态势,并比较中美创新能力。研究发现:全球医学科技已升级为关乎国家...本文基于Web of Science、Medline、Nature Index、高被引科学家名单及世卫组织、FDA、NMPA、IQVIA等最新数据,系统分析全球医学科技战略演变、基础研究格局与医药研发态势,并比较中美创新能力。研究发现:全球医学科技已升级为关乎国家安全、生物经济与供应链韧性的战略体系,病原体监测、疫苗平台、生物制造成为关键方向。基础研究进入高位稳定期,美国在规模、机构与人才上仍占主导;中国在生物科学高质量科研份额上已反超美国,但在健康科学和临床研究仍偏弱。在医药研发层面,资本与并购加速向ADC、细胞与基因治疗、GLP-1和AI药物研发集中,新兴生物制药公司成为首创新药重要来源。中美均保持全球新药批准高位,但美国在首创机制与罕见病体系上优势明显。总体看,中国正迈向全球医学创新关键力量,但仍需补强健康科学、临床转化与平台体系建设。展开更多
基金supported by the Robert A.Welch Foundation(Y0026)the French National Center for Scientific Research(ISA-CNRS-UMR5280)
文摘Current trends in chiral analysis of pharmaceutical drugs are focused on faster separations and higher separation efficiencies, Core-shell or superficially porous particles （SPP） based chiral stationary phases （CSPs） provide reduced analysis times while maintaining high column efficiencies and sensitivity. In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 μm SPP based vancomycin CSP （SPP-V） 10 cm ×0.21 cm column was compared to that of a corresponding 5 μm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2-5 time greater sensitivity and shorter analysis time for a set of 22 basic pharma- ceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racemic citalopram whose enantiomers could be selectively identified by MS.
文摘Detection of drugs in hair has become popular in recent years. The significantly long drug detection window (months) in hair has allowed the retrospective investigation and measurement of past consumption of drug. As the majority of drugs are basic, an extraction method was developed based on a methanolic solution for detection of basic/weak basic drugs in hair. It was compared with alkaline digestion (NaOH) followed by LLE. A filtration step with filtration vials was added and their materials were compared. After filtration, extracts were injected directly onto a C18 column coupled to Sciex ABI 2000 MSMS. The mobile phase was 50% methanol, 0.1% formic acid and 2 mM ammonium acetate (isocratic). Both methods were compared by applying them to real samples. Results showed that calibration was linear with r2 of 0.991-0.999 for 20 tested analytes. The matrix effect was assessed to be between 91.4%- 110.2% for 18 analytes. PTFE filter material showed better recoveries over the GMF and PVDF based filters. Stability of analytes during extraction in general was better with methanolic incubation rather than alkaline digestion. With regard to real sample recovery, 6 out of 10 analytes recovered better with alkaline digestion. In conclusion, the methanolic method is capable of extracting most basic drugs in hair samples but only part of the total incorporated drug. Therefore, these results suggest that a combination of both methods (methanolic and alkaline extractions) in hair sample processing for general detection of basic and weak basic drugs may produce better results. However, not all basic drugs are stable under alkaline digestion.
基金theNationalNaturalScienceFoundationofChina (No .2 0 175 0 10 )the 14thNationalKeyLaboratoryOpeningFounda ionofChina (No .2 0 12 0 3 )
文摘A novel pressurized capillary electrochromatography (PCEC) was developed to separate basic drugs on strong cation exchange (SCX) column. The separation result by using PCEC was better than that by using micro-HPLC. The effects of electrical field and pressure on plate height and resolution were investigated. Influences of organic modifier, ionic strength and pH value of buffer on retention behavior were evaluated, and the separation mechanism was also discussed.
文摘Basic research features its indelible contribution to and energetic promotion of scientific cause and advancing social progress. This article explores its vital position and rewarding role in creating socio-economic benefits by reviewing the development and marketing success of a new drug, "Di’ aoxinxuekang".
文摘本文基于Web of Science、Medline、Nature Index、高被引科学家名单及世卫组织、FDA、NMPA、IQVIA等最新数据,系统分析全球医学科技战略演变、基础研究格局与医药研发态势,并比较中美创新能力。研究发现:全球医学科技已升级为关乎国家安全、生物经济与供应链韧性的战略体系,病原体监测、疫苗平台、生物制造成为关键方向。基础研究进入高位稳定期,美国在规模、机构与人才上仍占主导;中国在生物科学高质量科研份额上已反超美国,但在健康科学和临床研究仍偏弱。在医药研发层面,资本与并购加速向ADC、细胞与基因治疗、GLP-1和AI药物研发集中,新兴生物制药公司成为首创新药重要来源。中美均保持全球新药批准高位,但美国在首创机制与罕见病体系上优势明显。总体看,中国正迈向全球医学创新关键力量,但仍需补强健康科学、临床转化与平台体系建设。
