This paper introduces the system structure and work principle of the upgraded real time information system in Wangting Power Plant, and then expounds the realization way and function features of this system on B/S co...This paper introduces the system structure and work principle of the upgraded real time information system in Wangting Power Plant, and then expounds the realization way and function features of this system on B/S computing mode. The results of field application show the new system has good capability, reliability and expandability.展开更多
Evolving From principal-subordinate structure of C /S to flexible multileveled distributed structure, i.e. B/S architecture so as to form a wide, distributed and orderly Internet/Intranet integrated management inf orm...Evolving From principal-subordinate structure of C /S to flexible multileveled distributed structure, i.e. B/S architecture so as to form a wide, distributed and orderly Internet/Intranet integrated management inf ormation system, is the trend of development of application software of the whol e world. Advantages and disadvantages of the two modes: C/S and B/S are compared . It is pointed out that at present onefold B/S mode cannot yet fully fullfil th e demands of some complicated data processing, information statistics and analys is, etc, therefore it still awaits further development of technology to achieve 100% Internet. In this paper, a mode of B/S-C/S-blended multileveled architect ure is discussed; the logical levels of B/S mode is studied; Furthermore, combin ing some up-to-date software developing technology, combinatorial design of di fferent database application technology based on B/S is discussed. The expatiati on is made with following points: (1) Organic combination of the network distrib uted object technology of CORBA and the computation technology among different n etwork operating system ─ Java; (2) Organic combination of two modes of ASP and Plug_in; (3) JDBC Server and JDBC Client; (4) An expanded B/S model, i.e. the c lient application of browser communicates directly with Web server, and the serv er application communicates directly with database server through middle ware.展开更多
According to the necessity of flexible workflow management system, the solution to set up the visualized workflow modelling system based on B/S structure is put forward, which conforms to the relevant specifications o...According to the necessity of flexible workflow management system, the solution to set up the visualized workflow modelling system based on B/S structure is put forward, which conforms to the relevant specifications of WfMC and the workflow process definition meta-model. The design for system structure is presented in detail, and the key technologies for system implementation are also introduced. Additionally, an example is illustrated to demonstrate the validity of system.展开更多
through the design of teaching aerobics web-based course and application practice, the instructional design process based on B/ S learning are analyzed, and discusses several key problems in the design and teaching. T...through the design of teaching aerobics web-based course and application practice, the instructional design process based on B/ S learning are analyzed, and discusses several key problems in the design and teaching. This paper presents the resource based learning aerobics teaching design network course should adapt to the application mode of network teaching and traditional classroom combination; that will provide teaching demonstration, teaching contents for students as well as the function of self testing evaluation and provide the job management, teacher online Q ＆ A, database and knowledge base construction, greatly improving the ＂aerobics＂ teaching efficiency.展开更多
This paper deeply studies the intelligent Home embedded system based on B/S architecture. Firstly, analysis requirement of Home function based on the existing software development mode, and proposed a system framework...This paper deeply studies the intelligent Home embedded system based on B/S architecture. Firstly, analysis requirement of Home function based on the existing software development mode, and proposed a system framework of intelligent Home system, and designed the communication scheme of the whole Home system, also gives the system each function module realization. This paper achieve two basic service system based on Intelligent Home through a series of analysis and design： outdoor remote intelligent Home monitoring service and the indoor intelligent management service.展开更多
This paper detailed describes the design of intelligent Home Furnishing system based on B/S architecture. According to the existing development mode of intelligent Home Furnishing system and the analysis of functional...This paper detailed describes the design of intelligent Home Furnishing system based on B/S architecture. According to the existing development mode of intelligent Home Furnishing system and the analysis of functional requirements, the paper puts forward the general framework of intelligent Home Furnishing system, and designed the communication scheme of the system, and gives the system each function module realization. This paper mainly completes the design of networking, each function module of embedded intelligent family gateway server in the system building and CGI intelligent interactive program and CGI interactive program. Experiments show that each function module of the system meet to design requirements, the overall system can satisfy the actual demand Home Furnishing user, validated the feasibility of the B/S architecture in intelligent Home Furnishing system.展开更多
There is curiosity and awareness throughout the world regarding the role of Information and Communication technologies. This is felt in each and every section of society. Several studies have confirmed and considered ...There is curiosity and awareness throughout the world regarding the role of Information and Communication technologies. This is felt in each and every section of society. Several studies have confirmed and considered information and communication technology’s significance in the field of education. It has not only affected learners but also to the teachers. This paper explores how ICT-based projects affect teachers’ and students’ attitudes. The data was collected through self-prepared attitude scale. It was distributed among the teachers and students of various schools. Two hundred students and one hundred twenty teachers responded to the questionnaire. Analysis was done through the data collected from the teachers as well as from students. The study’s conclusions demonstrated that while there was no significant variation in the attitudes of teachers utilizing different ICT-based programs, there was a substantial difference in the students’ attitude toward learning with different ICT-based programs.展开更多
Dear Editor,Group B coxsackieviruses(CVBs),belonging to the genus Enterovirus(EV)of the family Picornaviridae,comprise six serotypes and share a typical picornaviral structure(Alhazmi et al.,2023).While most CVB infec...Dear Editor,Group B coxsackieviruses(CVBs),belonging to the genus Enterovirus(EV)of the family Picornaviridae,comprise six serotypes and share a typical picornaviral structure(Alhazmi et al.,2023).While most CVB infections are mild and self-limiting,they can cause severe or fatal illness,especially in children(Tracy and Gauntt,2008).展开更多
In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its nea...In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.展开更多
Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban constr...Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban construction land use planning and management from the angle of methodology. As a widely used technique, traditional multi-criteria evaluation based on GIS （MCE-GIS）, is not suitable for UCLSE. This study develops an improved MCE-GIS method which could be more suitable for UCLSE based on urban complex ecological system theory and the summary of the shortcomings of traditional MCE-GIS. The improvements include three aspects： a composite evaluation index system rather than natural indexes alone, an index weight calculated by using fuzzy Analytic Hierarchy Process （AHP） method rather than the common AHP method, and the integrated overlay rule, which includes selecting the minimum value, weighted linear combination （WLC） and simple summation. The main advantage of this improved technique is that it can make UCLSE more comprehensive, more operational and more reasonable. It can provide a scientific basis for decision making in the planning and management of urban construction land use. The improved MCE-GIS system has been adopted in the New Hefei City, Anhui Province, China. Based on the results of UCLSE in New Hefei, three functional areas including construction-appropriate areas, construction-restricted areas and construction-forbidden areas could be worked out, in which 36.90% of the total study area could be developed as urban construction land and the remaining 63.10% should be protected as reserves land or as ecological land. Furthermore, the results can orovide scientific decision suooort for spatial planning and eco-environment nrotection in New Hefei.展开更多
We propose a content-based parallel image retrieval system to achieve high responding ability. Our system is developed on cluster architectures. It has several retrieval. servers to supply the service of content-based...We propose a content-based parallel image retrieval system to achieve high responding ability. Our system is developed on cluster architectures. It has several retrieval. servers to supply the service of content-based image retrieval. It adopts the Browser/Server (B/S) mode. The users could visit our system though web pages. It uses the symmetrical color-spatial features (SCSF) to represent the content of an image. The SCSF is effective and efficient for image matching because it is independent of image distortion such as rotation and flip as well as it increases the matching accuracy. The SCSF was organized by M-tree, which could speedup the searching procedure. Our experiments show that the image matching is quickly and efficiently with the use of SCSF. And with the support of several retrieval servers, the system could respond to many users at mean time. Key words content-based image retrieval - cluster architecture - color-spatial feature - B/S mode - task parallel - WWW - Internet CLC number TP391 Foundation item: Supported by the National Natural Science Foundation of China (60173058)Biography: ZHOU Bing (1975-), male, Ph. D candidate, reseach direction: data mining, content-based image retrieval.展开更多
Models of mass transfer kinetics combined with mass transfer differential equation and mass transfer resistance equation were established on the basis of double-film theory. Mass transfer process of H2 S absorption by...Models of mass transfer kinetics combined with mass transfer differential equation and mass transfer resistance equation were established on the basis of double-film theory. Mass transfer process of H2 S absorption by means of polypropylene hydrophobic microporous hollow fiber membrane contactor was simulated using MDEA(N-methyldiethanolamine) as the absorption liquid and corresponding experiments of natural gas desulfurization were performed. The simulation results indicated that the removal rate of hydrogen sulfide showed positive dependence on the absorption liquid concentration and gas pressure. However, the desulfurization rate showed negative dependence on gas flow. The simulated values were in good agreement with the experimental results. The in-tube concentration of hydrogen sulfide at the same point increased with increase in the gas velocity. Axial concentration of hydrogen sulfide decreased rapidly at the beginning, and the decrease saw a slowdown during the latter half period. Hydrogen sulfide concentration dropped quickly in the radial direction, and the reduction in the radial direction was weakened with the increase of axial length due to the gradual reduction of hydrogen sulfide concentration along the tube. The desulfurization rate under given operating conditions can be predicted by this model, and the theoretical basis for membrane module design can also be provided.展开更多
Based on isotropie linear poroelastic theory and under the undrained condition, we summarize three equations connecting the Skempton＇s coefficient B with the groundwater level. After analysis, we propose a method to ...Based on isotropie linear poroelastic theory and under the undrained condition, we summarize three equations connecting the Skempton＇s coefficient B with the groundwater level. After analysis, we propose a method to calculate the Skempton＇s coefficient B according to the relationship between water level and tidal strain. With this method we can get the value of B without the earthquake occurrence, which can provide the high frequency waves for research. Besides, we can also get the in-suit Skempton＇s coefficient B without the experiment of rock physics. In addition, we analyze the observed data of Changping station recorded in groundwater monitoring network （abv., GMN） before and after the Wenchuan Ms8.0 with this method, and find out there＇s a slight change of the value of B after the seismic waves passed by, which implies that the propagation of seismic waves may have brought some variations to the poroelastic medium of the well.展开更多
Aiming at the problem of low surface defect detection accuracy of industrial products, an object detection method based on simplified spatial pyramid pooling fast(Sim SPPF) hybrid pooling improved you only look once v...Aiming at the problem of low surface defect detection accuracy of industrial products, an object detection method based on simplified spatial pyramid pooling fast(Sim SPPF) hybrid pooling improved you only look once version 5s(YOLOV5s) model is proposed. The algorithm introduces channel attention(CA) module, simplified SPPF feature vector pyramid and efficient intersection over union(EIOU) loss function. Feature vector pyramids fuse high-dimensional and low-dimensional features, which makes semantic information richer. The CA mechanism performs maximum pooling and average pooling operations on the feature map. Hybrid pooling comprehensively improves detection computing efficiency and accurate deployment ability. The results show that the improved YOLOV5s model is better than the original YOLOV5s model. The average test accuracy(mAP) can reach 91.8%, which can be increased by 17.4%, and the detection speed can reach 108 FPS, which can be increased by 18 FPS. The improved model is practicable, and the overall performance is better than other conventional models.展开更多
AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared...AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.展开更多
文摘This paper introduces the system structure and work principle of the upgraded real time information system in Wangting Power Plant, and then expounds the realization way and function features of this system on B/S computing mode. The results of field application show the new system has good capability, reliability and expandability.
文摘Evolving From principal-subordinate structure of C /S to flexible multileveled distributed structure, i.e. B/S architecture so as to form a wide, distributed and orderly Internet/Intranet integrated management inf ormation system, is the trend of development of application software of the whol e world. Advantages and disadvantages of the two modes: C/S and B/S are compared . It is pointed out that at present onefold B/S mode cannot yet fully fullfil th e demands of some complicated data processing, information statistics and analys is, etc, therefore it still awaits further development of technology to achieve 100% Internet. In this paper, a mode of B/S-C/S-blended multileveled architect ure is discussed; the logical levels of B/S mode is studied; Furthermore, combin ing some up-to-date software developing technology, combinatorial design of di fferent database application technology based on B/S is discussed. The expatiati on is made with following points: (1) Organic combination of the network distrib uted object technology of CORBA and the computation technology among different n etwork operating system ─ Java; (2) Organic combination of two modes of ASP and Plug_in; (3) JDBC Server and JDBC Client; (4) An expanded B/S model, i.e. the c lient application of browser communicates directly with Web server, and the serv er application communicates directly with database server through middle ware.
基金Shanghai Municipal Science Committee key project(061612058,06JC14066,06DZ12001,061111006)Nationalscience and technology supporting project(2006BAF01A46)
文摘According to the necessity of flexible workflow management system, the solution to set up the visualized workflow modelling system based on B/S structure is put forward, which conforms to the relevant specifications of WfMC and the workflow process definition meta-model. The design for system structure is presented in detail, and the key technologies for system implementation are also introduced. Additionally, an example is illustrated to demonstrate the validity of system.
文摘through the design of teaching aerobics web-based course and application practice, the instructional design process based on B/ S learning are analyzed, and discusses several key problems in the design and teaching. This paper presents the resource based learning aerobics teaching design network course should adapt to the application mode of network teaching and traditional classroom combination; that will provide teaching demonstration, teaching contents for students as well as the function of self testing evaluation and provide the job management, teacher online Q ＆ A, database and knowledge base construction, greatly improving the ＂aerobics＂ teaching efficiency.
文摘This paper deeply studies the intelligent Home embedded system based on B/S architecture. Firstly, analysis requirement of Home function based on the existing software development mode, and proposed a system framework of intelligent Home system, and designed the communication scheme of the whole Home system, also gives the system each function module realization. This paper achieve two basic service system based on Intelligent Home through a series of analysis and design： outdoor remote intelligent Home monitoring service and the indoor intelligent management service.
文摘This paper detailed describes the design of intelligent Home Furnishing system based on B/S architecture. According to the existing development mode of intelligent Home Furnishing system and the analysis of functional requirements, the paper puts forward the general framework of intelligent Home Furnishing system, and designed the communication scheme of the system, and gives the system each function module realization. This paper mainly completes the design of networking, each function module of embedded intelligent family gateway server in the system building and CGI intelligent interactive program and CGI interactive program. Experiments show that each function module of the system meet to design requirements, the overall system can satisfy the actual demand Home Furnishing user, validated the feasibility of the B/S architecture in intelligent Home Furnishing system.
文摘There is curiosity and awareness throughout the world regarding the role of Information and Communication technologies. This is felt in each and every section of society. Several studies have confirmed and considered information and communication technology’s significance in the field of education. It has not only affected learners but also to the teachers. This paper explores how ICT-based projects affect teachers’ and students’ attitudes. The data was collected through self-prepared attitude scale. It was distributed among the teachers and students of various schools. Two hundred students and one hundred twenty teachers responded to the questionnaire. Analysis was done through the data collected from the teachers as well as from students. The study’s conclusions demonstrated that while there was no significant variation in the attitudes of teachers utilizing different ICT-based programs, there was a substantial difference in the students’ attitude toward learning with different ICT-based programs.
基金supported by grants from the National Natural Science Foundation of China(82172248,82472253,82272310 and 32470996)the Xiamen Science and Technology Program(2022CXY0102)+1 种基金the Fundamental Research Funds for the Central Universities(20720250004)the Independent Research Project of the State Key Laboratory of Vaccine for Infectious Diseases(2024SKLVDzy03).
文摘Dear Editor,Group B coxsackieviruses(CVBs),belonging to the genus Enterovirus(EV)of the family Picornaviridae,comprise six serotypes and share a typical picornaviral structure(Alhazmi et al.,2023).While most CVB infections are mild and self-limiting,they can cause severe or fatal illness,especially in children(Tracy and Gauntt,2008).
文摘In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.
基金Under the auspices of National Natural Science Foundation of China(No.41201168)Fundamental Research Funds for the Central Universities(No.2013HGXJ0207)
文摘Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban construction land use planning and management from the angle of methodology. As a widely used technique, traditional multi-criteria evaluation based on GIS （MCE-GIS）, is not suitable for UCLSE. This study develops an improved MCE-GIS method which could be more suitable for UCLSE based on urban complex ecological system theory and the summary of the shortcomings of traditional MCE-GIS. The improvements include three aspects： a composite evaluation index system rather than natural indexes alone, an index weight calculated by using fuzzy Analytic Hierarchy Process （AHP） method rather than the common AHP method, and the integrated overlay rule, which includes selecting the minimum value, weighted linear combination （WLC） and simple summation. The main advantage of this improved technique is that it can make UCLSE more comprehensive, more operational and more reasonable. It can provide a scientific basis for decision making in the planning and management of urban construction land use. The improved MCE-GIS system has been adopted in the New Hefei City, Anhui Province, China. Based on the results of UCLSE in New Hefei, three functional areas including construction-appropriate areas, construction-restricted areas and construction-forbidden areas could be worked out, in which 36.90% of the total study area could be developed as urban construction land and the remaining 63.10% should be protected as reserves land or as ecological land. Furthermore, the results can orovide scientific decision suooort for spatial planning and eco-environment nrotection in New Hefei.
文摘We propose a content-based parallel image retrieval system to achieve high responding ability. Our system is developed on cluster architectures. It has several retrieval. servers to supply the service of content-based image retrieval. It adopts the Browser/Server (B/S) mode. The users could visit our system though web pages. It uses the symmetrical color-spatial features (SCSF) to represent the content of an image. The SCSF is effective and efficient for image matching because it is independent of image distortion such as rotation and flip as well as it increases the matching accuracy. The SCSF was organized by M-tree, which could speedup the searching procedure. Our experiments show that the image matching is quickly and efficiently with the use of SCSF. And with the support of several retrieval servers, the system could respond to many users at mean time. Key words content-based image retrieval - cluster architecture - color-spatial feature - B/S mode - task parallel - WWW - Internet CLC number TP391 Foundation item: Supported by the National Natural Science Foundation of China (60173058)Biography: ZHOU Bing (1975-), male, Ph. D candidate, reseach direction: data mining, content-based image retrieval.
基金financially supported by the National Natual Science Foundation of China(No.51176015)
文摘Models of mass transfer kinetics combined with mass transfer differential equation and mass transfer resistance equation were established on the basis of double-film theory. Mass transfer process of H2 S absorption by means of polypropylene hydrophobic microporous hollow fiber membrane contactor was simulated using MDEA(N-methyldiethanolamine) as the absorption liquid and corresponding experiments of natural gas desulfurization were performed. The simulation results indicated that the removal rate of hydrogen sulfide showed positive dependence on the absorption liquid concentration and gas pressure. However, the desulfurization rate showed negative dependence on gas flow. The simulated values were in good agreement with the experimental results. The in-tube concentration of hydrogen sulfide at the same point increased with increase in the gas velocity. Axial concentration of hydrogen sulfide decreased rapidly at the beginning, and the decrease saw a slowdown during the latter half period. Hydrogen sulfide concentration dropped quickly in the radial direction, and the reduction in the radial direction was weakened with the increase of axial length due to the gradual reduction of hydrogen sulfide concentration along the tube. The desulfurization rate under given operating conditions can be predicted by this model, and the theoretical basis for membrane module design can also be provided.
基金supported by National Natural Science Foundation of China(40674024 and 40374019)
文摘Based on isotropie linear poroelastic theory and under the undrained condition, we summarize three equations connecting the Skempton＇s coefficient B with the groundwater level. After analysis, we propose a method to calculate the Skempton＇s coefficient B according to the relationship between water level and tidal strain. With this method we can get the value of B without the earthquake occurrence, which can provide the high frequency waves for research. Besides, we can also get the in-suit Skempton＇s coefficient B without the experiment of rock physics. In addition, we analyze the observed data of Changping station recorded in groundwater monitoring network （abv., GMN） before and after the Wenchuan Ms8.0 with this method, and find out there＇s a slight change of the value of B after the seismic waves passed by, which implies that the propagation of seismic waves may have brought some variations to the poroelastic medium of the well.
基金supported by the Tianjin Postgraduate Research Innovation Project (No.2022SKY286)the National Science and the National Key Research and Development Program (No.2022YFF0706000)。
文摘Aiming at the problem of low surface defect detection accuracy of industrial products, an object detection method based on simplified spatial pyramid pooling fast(Sim SPPF) hybrid pooling improved you only look once version 5s(YOLOV5s) model is proposed. The algorithm introduces channel attention(CA) module, simplified SPPF feature vector pyramid and efficient intersection over union(EIOU) loss function. Feature vector pyramids fuse high-dimensional and low-dimensional features, which makes semantic information richer. The CA mechanism performs maximum pooling and average pooling operations on the feature map. Hybrid pooling comprehensively improves detection computing efficiency and accurate deployment ability. The results show that the improved YOLOV5s model is better than the original YOLOV5s model. The average test accuracy(mAP) can reach 91.8%, which can be increased by 17.4%, and the detection speed can reach 108 FPS, which can be increased by 18 FPS. The improved model is practicable, and the overall performance is better than other conventional models.
基金the National Natural Science Foundation of China,No.39789010
文摘AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.
