Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri(M. barteri).Methods: Antimicrobial activity was carried out using micro-dilution, c...Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri(M. barteri).Methods: Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and chemical constituents of the extracts respectively.Results: Marked activity was observed in methanol(ME) fraction [MIC_(50):(0.097 7–6.250 0) mg/mL] compared to hexane and ethyl acetate fractions. Aeromonas hydrophila(environmental strain) and Shigella sonnei(ATCC 29930) were the most susceptible pathogens to ME and ciprofloxacin(Cl) at MIC_(50) value of 0.097 7 and < 0.019 5 mg/mL respectively. Cryptococcus neoformans(ATCC 66031) was susceptible to ME at 0.195 3 mg/mL compared to fluconazole at 10.000 0 mg/mL. Decreased viability of the Vero cells was observed at the concentrations of 0.1–1.0 mg/mL. The lethal dose(LC_(50))of hexane, ethyl acetate and methanol fractions were recorded at(0.30 ± 0.07),(0.52 ± 0.05) and(0.22 ± 0.04) mg/mL, respectively. Some of the compounds identified from ME were caryophyllene(25.21%), neophytadiene(11.90%), a-humulene(7.67%),phytol(4.40%), ethyl ester hexadecanoic acid(4.04%) and nerolidol(2.83%) which were known to have various antimicrobial activities.Conclusions: Methanol fraction of M. barteri is a potent and safe antimicrobial and antifungal alternative which can be useful in the search for new antimicrobial drugs. The study also confirmed the orthodox usage of M. barteri in combating infectious diseases.展开更多
Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadox...Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine(1.2 mg/kg), aqueous extract(30, 100, 300 mg/kg) and dichloromethane/methanol(D/M)(30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine(4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant(P 【 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative(P 【 0.05) but not prophylactic antiplasmodial ef ect. The aqueous extract exhibited in vitro antioxidant property with IC50’s of(0.930 ± 0.021) mg/mL,(0.800 ± 0.001) mg/mL and(0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity.Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial ef ects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property.展开更多
Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis bar...Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis barteri Hook.F.(Anacardiaceae)in Benin(West Africa)was studied,using 11 traits related to panicles,fruits,leaves and leaflets.A total of 1,485 panicles,1,485 fruits,990 leaves and 4,950 leaflets were sampled.It was found that the sample provenance and site topography have a significant influence on the morphological traits.The discriminating morphological traits were the length of the panicles,the number of fruits per panicle,the length and width of fruits,the mass of the fresh fruit pulp mass,the length of leaves and petioles,the number of leaflets per leaf,length and width of leaflets.On the basis of these traits,four different morphotypes of H.barteri were identified,with however a small intra and inter group variability.The morphotype from sites established on the tops and the high slopes of hill produced larger and heavier fruits with higher pulp mass.This morphotype could be of interest for future varietal selection programs for the species in Benin.展开更多
In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extra...In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extract of the stem bark of Lannea barteri and its shea butter formulated ointment using an excision wound model in the rabbit. The herbal ointment (5%, w/w) exhibited a significant wound healing activity, showing 99.9% ± 0.3% wound contraction at the end of the experiment (24th day). There was no significant difference (p > 0.05) between the 5% (w/w) herbal ointment compared with Silver Sulphadiazine cream (positive control), which showed a 97.8% ± 1.0% rate of excision wound contraction on day 24. Compared with the negative control group (administered with only shea butter), the wound healing activity of the ointment was significant (p Lannea barteri for the treatment of wounds and demonstrates that shea butter usage as a base in formulating an herbal ointment might aid in topical application for wound repair and regeneration, as well as the potential for enhanced wound healing.展开更多
采用稀释涂板法,对小水榕茎部的内生菌进行分离、纯化及16S r DNA鉴定,通过同源性比对构建系统发育树。结果表明:分离到的24株内生菌分别属于5个类群(Alphaproteobacteria、Gammaproteobacteria、Firmicutes、Bacteroidetes、Filimonas)...采用稀释涂板法,对小水榕茎部的内生菌进行分离、纯化及16S r DNA鉴定,通过同源性比对构建系统发育树。结果表明:分离到的24株内生菌分别属于5个类群(Alphaproteobacteria、Gammaproteobacteria、Firmicutes、Bacteroidetes、Filimonas)的11个属(Phenylobacterium、Rhizobium、Caulobacter、Sphingobium、Paenibacillus、Staphylococcus、Bacillus、Pseudomona、Chitinophaga sancti、Filimonas、Agrobacterium),其中Alphaproteobacteria、Firmicutes为优势种群,占总数的45.8%;所有菌株与其同源菌株的相似性均在98%以上。本研究有助于揭示小水榕茎部内生菌的多样性,为发掘其应用潜力提供参考资料。展开更多
基金the Directorate Research Development,UFS,South Africa for the Postdoctoral Fellow-ship granted Dr.A.L Ogundajo(Entity No.2114 B5004)tenable at the Phytomedicine and Phytopharmacology Research Unit,UFS,South Africa.
文摘Objectives: To investigate antimicrobial and cytotoxic potentials as well as chemical constituents of extracts from Macaranga barteri(M. barteri).Methods: Antimicrobial activity was carried out using micro-dilution, cell culture and GC–MS methods were employed to determine the cytotoxicity and chemical constituents of the extracts respectively.Results: Marked activity was observed in methanol(ME) fraction [MIC_(50):(0.097 7–6.250 0) mg/mL] compared to hexane and ethyl acetate fractions. Aeromonas hydrophila(environmental strain) and Shigella sonnei(ATCC 29930) were the most susceptible pathogens to ME and ciprofloxacin(Cl) at MIC_(50) value of 0.097 7 and < 0.019 5 mg/mL respectively. Cryptococcus neoformans(ATCC 66031) was susceptible to ME at 0.195 3 mg/mL compared to fluconazole at 10.000 0 mg/mL. Decreased viability of the Vero cells was observed at the concentrations of 0.1–1.0 mg/mL. The lethal dose(LC_(50))of hexane, ethyl acetate and methanol fractions were recorded at(0.30 ± 0.07),(0.52 ± 0.05) and(0.22 ± 0.04) mg/mL, respectively. Some of the compounds identified from ME were caryophyllene(25.21%), neophytadiene(11.90%), a-humulene(7.67%),phytol(4.40%), ethyl ester hexadecanoic acid(4.04%) and nerolidol(2.83%) which were known to have various antimicrobial activities.Conclusions: Methanol fraction of M. barteri is a potent and safe antimicrobial and antifungal alternative which can be useful in the search for new antimicrobial drugs. The study also confirmed the orthodox usage of M. barteri in combating infectious diseases.
文摘Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine(1.2 mg/kg), aqueous extract(30, 100, 300 mg/kg) and dichloromethane/methanol(D/M)(30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine(4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant(P 【 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative(P 【 0.05) but not prophylactic antiplasmodial ef ect. The aqueous extract exhibited in vitro antioxidant property with IC50’s of(0.930 ± 0.021) mg/mL,(0.800 ± 0.001) mg/mL and(0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity.Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial ef ects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property.
文摘Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis barteri Hook.F.(Anacardiaceae)in Benin(West Africa)was studied,using 11 traits related to panicles,fruits,leaves and leaflets.A total of 1,485 panicles,1,485 fruits,990 leaves and 4,950 leaflets were sampled.It was found that the sample provenance and site topography have a significant influence on the morphological traits.The discriminating morphological traits were the length of the panicles,the number of fruits per panicle,the length and width of fruits,the mass of the fresh fruit pulp mass,the length of leaves and petioles,the number of leaflets per leaf,length and width of leaflets.On the basis of these traits,four different morphotypes of H.barteri were identified,with however a small intra and inter group variability.The morphotype from sites established on the tops and the high slopes of hill produced larger and heavier fruits with higher pulp mass.This morphotype could be of interest for future varietal selection programs for the species in Benin.
文摘In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extract of the stem bark of Lannea barteri and its shea butter formulated ointment using an excision wound model in the rabbit. The herbal ointment (5%, w/w) exhibited a significant wound healing activity, showing 99.9% ± 0.3% wound contraction at the end of the experiment (24th day). There was no significant difference (p > 0.05) between the 5% (w/w) herbal ointment compared with Silver Sulphadiazine cream (positive control), which showed a 97.8% ± 1.0% rate of excision wound contraction on day 24. Compared with the negative control group (administered with only shea butter), the wound healing activity of the ointment was significant (p Lannea barteri for the treatment of wounds and demonstrates that shea butter usage as a base in formulating an herbal ointment might aid in topical application for wound repair and regeneration, as well as the potential for enhanced wound healing.
文摘采用稀释涂板法,对小水榕茎部的内生菌进行分离、纯化及16S r DNA鉴定,通过同源性比对构建系统发育树。结果表明:分离到的24株内生菌分别属于5个类群(Alphaproteobacteria、Gammaproteobacteria、Firmicutes、Bacteroidetes、Filimonas)的11个属(Phenylobacterium、Rhizobium、Caulobacter、Sphingobium、Paenibacillus、Staphylococcus、Bacillus、Pseudomona、Chitinophaga sancti、Filimonas、Agrobacterium),其中Alphaproteobacteria、Firmicutes为优势种群,占总数的45.8%;所有菌株与其同源菌株的相似性均在98%以上。本研究有助于揭示小水榕茎部内生菌的多样性,为发掘其应用潜力提供参考资料。
