Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an imag...Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an image analysis. Molecular techniques employing 16S and 28S rDNA profiles generated by PCR-DGGE were used to detect the variation in microbial community （bacteria, fungi） of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and Shea tree fruits from five different districts in Mali. The bacterial DNA profiles from Pangasius fish and the fungal DNA profile from Shea tree fruits were specific to each place of production and could be used as a biological bar code certifying the origin of fish and fruit. To follow the product during processing, the authors proposed to identify and validate some pertinent biological markers which come from the environment of the food to assure their traceability during international trade. It is new analytical method which permits to determine the origin of food or to follow them during international trade.展开更多
美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的...美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的序列进行PCR扩增和测序,将所得序列用于蓟马种类的分子鉴定。使用不同的矩阵和系统发育构建方法对待鉴定的蓟马以及另外38种蓟马进行了聚类分析,结果表明:基于线粒体COⅠ基因第1和2编码位点,利用NJ法构建的系统发育树可以较好地区别不同蓟马种类,可作为调查我国蓟马种类和分布的快速方便方法。最后对美洲棘蓟马的基本生物学特性和防控对策进行了综述。展开更多
为了让消费者购买牛肉后可以追溯到牛肉生产的全过程,实现牛肉质量安全可追溯系统的智能、快捷、实用的目标,引入"RF ID+条码"技术,对肉牛及生产的牛肉进行标识,跟踪牛肉全生产过程。并设计开发了网络化牛肉可追溯系统,系统采...为了让消费者购买牛肉后可以追溯到牛肉生产的全过程,实现牛肉质量安全可追溯系统的智能、快捷、实用的目标,引入"RF ID+条码"技术,对肉牛及生产的牛肉进行标识,跟踪牛肉全生产过程。并设计开发了网络化牛肉可追溯系统,系统采用JSP设计面向对象的动态网页,采用M y SQL设计数据库,运用B/S(浏览器/服务器)体系结构,将管理系统放在互联网上,实现了牛肉质量安全可追溯系统的网络化管理,使牛肉质量安全可追溯系统网络化、普及化。展开更多
文摘Abstract： One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an image analysis. Molecular techniques employing 16S and 28S rDNA profiles generated by PCR-DGGE were used to detect the variation in microbial community （bacteria, fungi） of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and Shea tree fruits from five different districts in Mali. The bacterial DNA profiles from Pangasius fish and the fungal DNA profile from Shea tree fruits were specific to each place of production and could be used as a biological bar code certifying the origin of fish and fruit. To follow the product during processing, the authors proposed to identify and validate some pertinent biological markers which come from the environment of the food to assure their traceability during international trade. It is new analytical method which permits to determine the origin of food or to follow them during international trade.
文摘美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的序列进行PCR扩增和测序,将所得序列用于蓟马种类的分子鉴定。使用不同的矩阵和系统发育构建方法对待鉴定的蓟马以及另外38种蓟马进行了聚类分析,结果表明:基于线粒体COⅠ基因第1和2编码位点,利用NJ法构建的系统发育树可以较好地区别不同蓟马种类,可作为调查我国蓟马种类和分布的快速方便方法。最后对美洲棘蓟马的基本生物学特性和防控对策进行了综述。
文摘为了让消费者购买牛肉后可以追溯到牛肉生产的全过程,实现牛肉质量安全可追溯系统的智能、快捷、实用的目标,引入"RF ID+条码"技术,对肉牛及生产的牛肉进行标识,跟踪牛肉全生产过程。并设计开发了网络化牛肉可追溯系统,系统采用JSP设计面向对象的动态网页,采用M y SQL设计数据库,运用B/S(浏览器/服务器)体系结构,将管理系统放在互联网上,实现了牛肉质量安全可追溯系统的网络化管理,使牛肉质量安全可追溯系统网络化、普及化。
