Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive ...Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive species,which can be regulated by the activation process.In this study,surface plasma-activated air(SAA)and a He+O_(2)plasma jet(Jet)were parallelly combined(the SAA+Jet combination)or sequentially combined(the SAA→Jet combination and the Jet→SAA combination)to prepare plasma-activated saline(PAS).The PAS activated by the combinations exhibited stronger bactericidal effects than that activated by the SAA or the Jet alone.The concentrations of H_(2)O_(2)and NO_(2)^(-)were higher in the PAS activated by the Jet→SAA combination,while ONOO^(-)concentrations were close in the three kinds of PAS and^(1)O_(2)concentrations were higher in the PAS activated by the SAA+Jet combination.The analysis of scavengers also demonstrated that H_(2)O_(2),^(1)O_(2),and ONOO^(-)in the PAS activated by the SAA+Jet combination,and^(1)O_(2)in the PAS activated by the Jet→SAA combination played critical roles in bactericidal effects.Further,the effective placement time of the three PAS varied,and the PAS activated by the Jet→SAA combination could also inactivate 2.6-log_(10)of MRSA cells after placement for more than 60 min.The regulation of reactive species in plasma-activated water via different combinations of plasma devices could improve the directional application of plasma-activated water in the biomedical field.展开更多
This study investigates the effect of apple extract on CoFe_(2)O_(4) nanoparticles synthesized via a green self-ignition method.High resolution transmission electron microscope(HRTEM)showed nanometric particles with v...This study investigates the effect of apple extract on CoFe_(2)O_(4) nanoparticles synthesized via a green self-ignition method.High resolution transmission electron microscope(HRTEM)showed nanometric particles with varied shapes,while X-ray diffraction(XRD)and Rietveld refinement confirmed a facecentered cubic(Fd3m)structure.Mössbauer spectroscopy revealed a Zeeman sextet pattern with only Fe^(3+) ions.Ultra violet vissible nearinfrared(UV-Vis-NIR)spectra indicated strong absorbance in the visible and NIR regions,suggesting optoelectronic potential.The nanoparticles demonstrated high photo-Fenton catalytic efficiency,degrading 96.88%of Methylene Blue under visible light.They also exhibited 100%adsorption capacities for Cr^(3+) and Pb^(2+),making them effective for water treatment.These properties were attributed to a large surface area(347.04 m2/g),mesoporous structure,and mixed spinel phase.ANOVA and Tukey’s honestly significant difference(HSD)tests confirmed that contact time and adsorbent dosage significantly affected pollutant removal.Additionally,strong antimicrobial activity highlighted their biotechnological relevance.The inclusion of apple extract enhanced structural and functional features,expanding application prospects in spin valves,magnetic recording,refrigeration,microwave technologies(C to Ku bands),optoelectronics,and biotechnology.Future work should explore the photo-Fenton degradation mechanism and optimize synthesis for scalable production,aiming to maximize their industrial utility.展开更多
This research was directed to understand the bactericidal effect of epidermal mucus of two Asian cat fish species viz.Clarias batrachus and Heteropneustes fossilis.Epidermal mucus extracts(raw and diluted)of both cat ...This research was directed to understand the bactericidal effect of epidermal mucus of two Asian cat fish species viz.Clarias batrachus and Heteropneustes fossilis.Epidermal mucus extracts(raw and diluted)of both cat fish species were tested against several Gram negative(Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumonia,A.hydrophila)and Gram positive bacterial strains(Bacillius cereus,Staphylococcus aureus,S.epidermidis)and antibacterial results were also compared with two standard antibiotics viz.amikacin and chloramphenicol used as positive control.An A.hydrophila challenge experiment was also performed on all selected test fish species to examine the change in the amount of mucus production and its bactericidal impact..Both epidermal mucus extracts(raw and diluted)of all selected normal and bacterial challenged test objects showed potent bactericidal effect against all pathogenic bacterial strains taken under study.However,former was more effective than later.Also raw epidermal mucus extracts of both normal and bacterial challenged cat fish species exhibited significantly higher ZOI values against all selected microbial strains than diluted mucus extracts and antibiotic chloramphenicol.Hence,these outcomes have clearly revealed that this cost effective natural product acquired from fishes is the key component of their defensive system.Therefore,it could be utilized as a novel‘antimicrobial’in human as well as veterinary sector for combating against several bacterial diseases.展开更多
In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The re...In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The results showed that the combined treatment of US and CN had a significant(P<0.05)synergistic effect on the inactivation of L.monocytogenes,with synergistic values(SV)of 1.345 and 1.325,respectively.US+CN disrupts mononucleosis cell membranes and increases their permeability,as demonstrated by confocal laser scanning microscopy,flow cytometry and cell content release assays.N-phenyl-1-naphthylamine,reactive oxygen species and malondialdehyde assays showed that the combined treatment accelerated cellular oxidative stress and peroxidation,thereby affecting membrane lipid homeostasis.Field emission scanning electron microscopy showed that US+CN caused cell membranes to dry out and crumble.In addition,US+CN effectively kill L.monocytogenes on the surface of cabbages,reduce the weight loss of cabbages during storage,and had no significant(P>0.05)effect on its color and hardness.This study demonstrated that US+CN has the potential to inactivate foodborne pathogens and improve the safety of fresh produce,providing theoretical possibilities for its application.展开更多
Gibberellic acid-stimulated Arabidopsis(GASA)proteins,also known as Snakin proteins,have emerged as key players in plant development and stress tolerance.The investigation of the role of TdGASA2 in host defense respon...Gibberellic acid-stimulated Arabidopsis(GASA)proteins,also known as Snakin proteins,have emerged as key players in plant development and stress tolerance.The investigation of the role of TdGASA2 in host defense responses revealed dynamic expression patterns under different stress conditions.TdGASA2 expression showed a consistent increase after wounding,methyl jasmonate(MeJA),and ethylene(ETP)treatments,peaking after 12-24 h for MeJA and ETP and after 1 h for pathogen elicitor(PA)treatment.Optimal expression conditions were determined,and the protein was successfully purified with a molecular weight of approximately 10 kDa.In vitro experiments demonstrated the potent antimicrobial activity of TdGASA2 against a range of pathogens,surpassing the efficacy of kanamycin.Minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)values confirmed its bactericidal and bacteriostatic activity against various bacteria.Time-kill assays also highlighted its efficacy against Listeria monocytogenes and Salmonella enterica,underscoring its potential as a therapeutic agent.Additionally,TdGASA2 exhibited superior DPPH radical-scavenging activity compared to ascorbic acid,indicating its potential as a natural antioxidant.In situ experiments revealed dose-dependent effects of TdGASA2 treatment on microbial growth in cheese,suggesting its efficacy as a preserva-tive to extend shelf life and enhance food safety.These findings underscore TdGASA2’s multifaceted potential in both antimicrobial and antioxidant applications,positioning it as a promising candidate for food preservation and health promotion.展开更多
Antimicrobial resistance has now become a very serious global public health problem. New drug discovery and development are urgently needed to combat the growing threat of multidrug-resistant (MDR) bacteria. The aim o...Antimicrobial resistance has now become a very serious global public health problem. New drug discovery and development are urgently needed to combat the growing threat of multidrug-resistant (MDR) bacteria. The aim of this study was to explore the potential application of three ferrocene-carborane derivatives as new promising agents to confront the problem of increasing antibiotic resistance. The results of agar diffusion bioassay, minimal inhibitory concentrations (MIC) testing and time-kill assay illustrate their broad-spectrum antimicrobial activities to both American Type Culture Collection (ATCC) control strains and MDR clinical isolates. It is evident that the relevant antimicrobial properties are all in a dose-dependent manner and gradually transform into a bactericidal effect from a bacteriostatic effect with the increasing of the drug concentration. Furthermore, these ferrocene-carborane derivatives have no/little toxic effect on normal cells like HELF cells and lead to little hemolysis at their MICs. This raises the possibility to develop novel antimicrobial drugs using these new ferrocene carborane derivants.展开更多
基金supported by National Natural Science Foundation of China(No.51977174)。
文摘Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive species,which can be regulated by the activation process.In this study,surface plasma-activated air(SAA)and a He+O_(2)plasma jet(Jet)were parallelly combined(the SAA+Jet combination)or sequentially combined(the SAA→Jet combination and the Jet→SAA combination)to prepare plasma-activated saline(PAS).The PAS activated by the combinations exhibited stronger bactericidal effects than that activated by the SAA or the Jet alone.The concentrations of H_(2)O_(2)and NO_(2)^(-)were higher in the PAS activated by the Jet→SAA combination,while ONOO^(-)concentrations were close in the three kinds of PAS and^(1)O_(2)concentrations were higher in the PAS activated by the SAA+Jet combination.The analysis of scavengers also demonstrated that H_(2)O_(2),^(1)O_(2),and ONOO^(-)in the PAS activated by the SAA+Jet combination,and^(1)O_(2)in the PAS activated by the Jet→SAA combination played critical roles in bactericidal effects.Further,the effective placement time of the three PAS varied,and the PAS activated by the Jet→SAA combination could also inactivate 2.6-log_(10)of MRSA cells after placement for more than 60 min.The regulation of reactive species in plasma-activated water via different combinations of plasma devices could improve the directional application of plasma-activated water in the biomedical field.
文摘This study investigates the effect of apple extract on CoFe_(2)O_(4) nanoparticles synthesized via a green self-ignition method.High resolution transmission electron microscope(HRTEM)showed nanometric particles with varied shapes,while X-ray diffraction(XRD)and Rietveld refinement confirmed a facecentered cubic(Fd3m)structure.Mössbauer spectroscopy revealed a Zeeman sextet pattern with only Fe^(3+) ions.Ultra violet vissible nearinfrared(UV-Vis-NIR)spectra indicated strong absorbance in the visible and NIR regions,suggesting optoelectronic potential.The nanoparticles demonstrated high photo-Fenton catalytic efficiency,degrading 96.88%of Methylene Blue under visible light.They also exhibited 100%adsorption capacities for Cr^(3+) and Pb^(2+),making them effective for water treatment.These properties were attributed to a large surface area(347.04 m2/g),mesoporous structure,and mixed spinel phase.ANOVA and Tukey’s honestly significant difference(HSD)tests confirmed that contact time and adsorbent dosage significantly affected pollutant removal.Additionally,strong antimicrobial activity highlighted their biotechnological relevance.The inclusion of apple extract enhanced structural and functional features,expanding application prospects in spin valves,magnetic recording,refrigeration,microwave technologies(C to Ku bands),optoelectronics,and biotechnology.Future work should explore the photo-Fenton degradation mechanism and optimize synthesis for scalable production,aiming to maximize their industrial utility.
文摘This research was directed to understand the bactericidal effect of epidermal mucus of two Asian cat fish species viz.Clarias batrachus and Heteropneustes fossilis.Epidermal mucus extracts(raw and diluted)of both cat fish species were tested against several Gram negative(Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumonia,A.hydrophila)and Gram positive bacterial strains(Bacillius cereus,Staphylococcus aureus,S.epidermidis)and antibacterial results were also compared with two standard antibiotics viz.amikacin and chloramphenicol used as positive control.An A.hydrophila challenge experiment was also performed on all selected test fish species to examine the change in the amount of mucus production and its bactericidal impact..Both epidermal mucus extracts(raw and diluted)of all selected normal and bacterial challenged test objects showed potent bactericidal effect against all pathogenic bacterial strains taken under study.However,former was more effective than later.Also raw epidermal mucus extracts of both normal and bacterial challenged cat fish species exhibited significantly higher ZOI values against all selected microbial strains than diluted mucus extracts and antibiotic chloramphenicol.Hence,these outcomes have clearly revealed that this cost effective natural product acquired from fishes is the key component of their defensive system.Therefore,it could be utilized as a novel‘antimicrobial’in human as well as veterinary sector for combating against several bacterial diseases.
基金supported by the National Natural Science Foundation of China(32272445)the Shenzhen Natural Science Foundation(JCYJ20230807111500001 and JCYJ20240813151818024)+1 种基金the Natural Science Foundation of Guangdong Province of China(2023A1515012728)the Deanship of Scientific Research(DSR)at King Faisal University under project no KFU250674.
文摘In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The results showed that the combined treatment of US and CN had a significant(P<0.05)synergistic effect on the inactivation of L.monocytogenes,with synergistic values(SV)of 1.345 and 1.325,respectively.US+CN disrupts mononucleosis cell membranes and increases their permeability,as demonstrated by confocal laser scanning microscopy,flow cytometry and cell content release assays.N-phenyl-1-naphthylamine,reactive oxygen species and malondialdehyde assays showed that the combined treatment accelerated cellular oxidative stress and peroxidation,thereby affecting membrane lipid homeostasis.Field emission scanning electron microscopy showed that US+CN caused cell membranes to dry out and crumble.In addition,US+CN effectively kill L.monocytogenes on the surface of cabbages,reduce the weight loss of cabbages during storage,and had no significant(P>0.05)effect on its color and hardness.This study demonstrated that US+CN has the potential to inactivate foodborne pathogens and improve the safety of fresh produce,providing theoretical possibilities for its application.
基金supported to the Deanship of Scientific Research at King Khalid University for funding this work through the large research Groups projects to Narjes Baazaoui(Project under grant number(RGP.2/88/45))funded by the Tunisian Ministry of Higher Education and Scientific Research(Program contract 2023-2026).
文摘Gibberellic acid-stimulated Arabidopsis(GASA)proteins,also known as Snakin proteins,have emerged as key players in plant development and stress tolerance.The investigation of the role of TdGASA2 in host defense responses revealed dynamic expression patterns under different stress conditions.TdGASA2 expression showed a consistent increase after wounding,methyl jasmonate(MeJA),and ethylene(ETP)treatments,peaking after 12-24 h for MeJA and ETP and after 1 h for pathogen elicitor(PA)treatment.Optimal expression conditions were determined,and the protein was successfully purified with a molecular weight of approximately 10 kDa.In vitro experiments demonstrated the potent antimicrobial activity of TdGASA2 against a range of pathogens,surpassing the efficacy of kanamycin.Minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)values confirmed its bactericidal and bacteriostatic activity against various bacteria.Time-kill assays also highlighted its efficacy against Listeria monocytogenes and Salmonella enterica,underscoring its potential as a therapeutic agent.Additionally,TdGASA2 exhibited superior DPPH radical-scavenging activity compared to ascorbic acid,indicating its potential as a natural antioxidant.In situ experiments revealed dose-dependent effects of TdGASA2 treatment on microbial growth in cheese,suggesting its efficacy as a preserva-tive to extend shelf life and enhance food safety.These findings underscore TdGASA2’s multifaceted potential in both antimicrobial and antioxidant applications,positioning it as a promising candidate for food preservation and health promotion.
基金supported by the National Natural Science Foundation of China (21175020)National Key Basic Research Program (2010CB732404)+4 种基金Doctoral Fund of Ministry of Education of China (20090092110028)National High Technology Research and Development Program(2007AA022007)Guangdong Province (2011B090400357)Graduate Research and Innovation Program of Jiangsu Province (CXLX_0145)State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences
文摘Antimicrobial resistance has now become a very serious global public health problem. New drug discovery and development are urgently needed to combat the growing threat of multidrug-resistant (MDR) bacteria. The aim of this study was to explore the potential application of three ferrocene-carborane derivatives as new promising agents to confront the problem of increasing antibiotic resistance. The results of agar diffusion bioassay, minimal inhibitory concentrations (MIC) testing and time-kill assay illustrate their broad-spectrum antimicrobial activities to both American Type Culture Collection (ATCC) control strains and MDR clinical isolates. It is evident that the relevant antimicrobial properties are all in a dose-dependent manner and gradually transform into a bactericidal effect from a bacteriostatic effect with the increasing of the drug concentration. Furthermore, these ferrocene-carborane derivatives have no/little toxic effect on normal cells like HELF cells and lead to little hemolysis at their MICs. This raises the possibility to develop novel antimicrobial drugs using these new ferrocene carborane derivants.
