Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil ...Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil of male and female Populus deltoides and bulk soil were collected from an 18-year plantation(male and female trees mix-planted)and grouped into three soil compartments.Soil carbon(C),nitrogen(N)and phosphorus(P)levels were determined,and soil bacterial communities were analyzed by high-throughput sequencing.The results showed the less total carbon and total organic carbon,the more nutrients(available phosphorus,nitrate nitrogen and ammonium nitrogen)available in the rhizosphere soils of female poplars than soils of males.However,α-diversity indices of the rhizosphere bacterial communities under male plants were signifi-cantly higher.Principal component analysis showed that the bacterial communities were signifi cantly diff erent between the male and female soil compartments.Further,the bacterial co-occurrence network in soil under male trees had more nodes and edges than under females.BugBase analysis showed the more functional bacteria taxa related to biofi lm formation and antioxidation under males.The results indicate that soils under male poplars had more diverse and more complex co-occurrence networks of the rhizosphere bacterial community than soils under female trees,implying that male poplars might have better environmental adaptability.The study provides insight into the diff erent soil-microbe interactions of dioecious plants.More details about the infl uencing mechanism of sexual dimorphism on rhizosphere soil bacterial communities need to be further studied.展开更多
Background No-tillage(NT)is a widely used field management to reduce soil erosion and degradation and is suggested to be beneficial for enhancing soil carbon(C)sequestration capacity.Nonetheless,the effects of NT on s...Background No-tillage(NT)is a widely used field management to reduce soil erosion and degradation and is suggested to be beneficial for enhancing soil carbon(C)sequestration capacity.Nonetheless,the effects of NT on soil total carbon(TC)content in aeolian sandy soils are not extensively explored,and the underlying mechanisms are not clear.In our field experiments,the influence of NT and conventional tillage(CT)on sandy soil was studied.Methods We estimated the changes in soil TC in response to NT practice in a Cyperus esculentus L.field located at semi-arid Horqin sandy land,China.To unravel the underlying mechanisms,plant traits,soil properties and soil microbial characteristics were measured in parallel.The variations in soil bacterial community structure were investigated by 16S rRNA amplicon sequencing.The functionality of soil bacterial community was predicted based on OTU tables by using PICRUSt2.Results NT increased soil TC content in this sandy agroecosystem within a short-term experimental period,compared to CT.The underlying mechanisms might rely on three aspects.First,NT increased soil TC content through increasing photosynthesis and plant biomass,and thus,the plant-derived dissolved organic C.Second,NT increased the C immobilized in soil microbial biomass by increasing microbial C demands and C use efficiency.Third,NT increased the dominance of oligotrophic members in bacterial communities by decreasing available nutrient levels,which is associated with the recalcitrance and stability of the soil organic carbon.Conclusions The present study enriched our knowledge on the changes in the plant-soil-microbe continuum in response to NT in a semi-arid sandy agroecosystem.Still,this study provides a reference for modifying tillage practices to benefit crop yield as well as soil C sequestration.展开更多
基金supported by the National Natural Science Foundation of China(32071751)the National key research and development program(2021YFD220120102)+1 种基金the Natural Science Foundation of Shandong Province(ZR2018ZC08N3)the funds of the Shandong Double Tops Program(Grant No.SYL2017XTTD03).
文摘Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil of male and female Populus deltoides and bulk soil were collected from an 18-year plantation(male and female trees mix-planted)and grouped into three soil compartments.Soil carbon(C),nitrogen(N)and phosphorus(P)levels were determined,and soil bacterial communities were analyzed by high-throughput sequencing.The results showed the less total carbon and total organic carbon,the more nutrients(available phosphorus,nitrate nitrogen and ammonium nitrogen)available in the rhizosphere soils of female poplars than soils of males.However,α-diversity indices of the rhizosphere bacterial communities under male plants were signifi-cantly higher.Principal component analysis showed that the bacterial communities were signifi cantly diff erent between the male and female soil compartments.Further,the bacterial co-occurrence network in soil under male trees had more nodes and edges than under females.BugBase analysis showed the more functional bacteria taxa related to biofi lm formation and antioxidation under males.The results indicate that soils under male poplars had more diverse and more complex co-occurrence networks of the rhizosphere bacterial community than soils under female trees,implying that male poplars might have better environmental adaptability.The study provides insight into the diff erent soil-microbe interactions of dioecious plants.More details about the infl uencing mechanism of sexual dimorphism on rhizosphere soil bacterial communities need to be further studied.
基金financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA 28060300)the National Key Research and Development Program of China(2019YFC0507601-03)the Applied Basic Research Programs of Liaoning Province(2023JH2/101700353)
文摘Background No-tillage(NT)is a widely used field management to reduce soil erosion and degradation and is suggested to be beneficial for enhancing soil carbon(C)sequestration capacity.Nonetheless,the effects of NT on soil total carbon(TC)content in aeolian sandy soils are not extensively explored,and the underlying mechanisms are not clear.In our field experiments,the influence of NT and conventional tillage(CT)on sandy soil was studied.Methods We estimated the changes in soil TC in response to NT practice in a Cyperus esculentus L.field located at semi-arid Horqin sandy land,China.To unravel the underlying mechanisms,plant traits,soil properties and soil microbial characteristics were measured in parallel.The variations in soil bacterial community structure were investigated by 16S rRNA amplicon sequencing.The functionality of soil bacterial community was predicted based on OTU tables by using PICRUSt2.Results NT increased soil TC content in this sandy agroecosystem within a short-term experimental period,compared to CT.The underlying mechanisms might rely on three aspects.First,NT increased soil TC content through increasing photosynthesis and plant biomass,and thus,the plant-derived dissolved organic C.Second,NT increased the C immobilized in soil microbial biomass by increasing microbial C demands and C use efficiency.Third,NT increased the dominance of oligotrophic members in bacterial communities by decreasing available nutrient levels,which is associated with the recalcitrance and stability of the soil organic carbon.Conclusions The present study enriched our knowledge on the changes in the plant-soil-microbe continuum in response to NT in a semi-arid sandy agroecosystem.Still,this study provides a reference for modifying tillage practices to benefit crop yield as well as soil C sequestration.
