Crude oil pollution is a significant global environmental challenge.The eastern Gansu Province on the Loess Plateau,an important agricultural region containing the Changqing Oilfield,is facing increasing crude oil con...Crude oil pollution is a significant global environmental challenge.The eastern Gansu Province on the Loess Plateau,an important agricultural region containing the Changqing Oilfield,is facing increasing crude oil contamination.Understanding how microbial communities respond to varying pollution levels is critical for developing effective bioremediation strategies.This study examined how different concentrations of crude oil affect soil properties and microbial communities in Qingyang City,eastern Gansu Province,China by comparing lightly polluted(1895.84-2696.54 mg/kg total petroleum hydrocarbons(TPH)),heavily polluted(4964.25-7153.61 mg/kg TPH),and uncontaminated(CK)soils.Results revealed that petroleum contamination significantly increased total organic carbon(TOC),pH,C:N:P ratio,and the activities of dehydrogenase(DHA)and polyphenol oxidase(PPO),while reducing total nitrogen(TN),available nitrogen(AN),total phosphorus(TP),available phosphorus(AP),available potassium(AK),soil organic matter(SOM),soil water content(SWC),the activities of urease(URE)and alkaline phosphatase(APA),and microbial alpha diversity(P<0.050).Light pollution(LP)soils demonstrated an increase in culturable microorganisms,whereas heavy pollution(HP)soils exhibited increased hydrocarbon-degrading microbes and higher expression of key functional genes,such as alkane monooxygenase(AlkB),cytochrome P450 alkane hydroxylases(P450),catechol 2,3-dioxygenase(C23O),and naphthalene dioxygenase(Nah)(P<0.050).Non-metric multidimensional scaling(NMDS)and redundancy analysis(RDA)indicated evident variations in microbial community structure across different oil contamination levels.LP soils were dominated by bacterial genera Pseudoxanthomonas and Solimonadaceae,whereas Pseudomonas,Nocardioides,and hydrocarbon-degrading genera(Marinobacter,Idiomarina,and Halomonas)were predominant in HP soils.The fungal genus Pseudallescheria exhibited the most pronounced abundance shift between LP and HP soils(P<0.050).Environmental factor analysis identified AN,SWC,TN,SOM,and alpha diversity indices(Shannon index and Chao1 index)as the key differentiators of CK soils,whereas the pollutant levels and metal content were characterized in HP soils.Hydrocarbon-degrading microbial abundance was a defining trait of HP soils.Metabolic pathway analysis revealed enhanced aromatic hydrocarbon degradation in HP soils,indicating microbial adaptation to severe contamination.These findings demonstrated that crude oil pollution suppressed soil nutrients while reshaping the structure and function of microbial communities.Pollution intensity directly affected microbial composition and degradation potential.This study offers valuable insights into microbial responses across contamination gradients and supports the development of targeted bioremediation strategies for oil-contaminated loess soils.展开更多
The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rD...The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach,and functional characteristics at community levels by using BIOLOG^(GN) system analysis method as well as two conventional methods(DHA and SIR).All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNA V_(3) high variable region universal primer)indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos,copper,especially some of their combinations had worse effects than their corresponding single factors.In addition,the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community.The effects of the single factors with lower concentrations on the communiy structure were weaker than those with higher concentrations.Moreover,the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors.The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOG^(GN)system were two relatively sensitive directors corresponding to the stress presented in this study.Between methamodophos and copper,there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities.The DHA of soil under the combined stresses was lower than that of the control and that under the single factors,and the BIOLOG^(GN) substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors.From all of above,the methamidophos,copper,especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure,richness,and the functional characteristics of microbial community.展开更多
基金supported by the Natural Science Foundation of Gansu Province(23JRRM0752,22JR5RA345,21JR1RM333)the Project of Science and Technology Specialist in Gansu Province(24CXGM002)+2 种基金the National Natural Science Foundation of China(31860148)the Research Fund Project for PhD of Longdong University(XYBYZK2208)the Natural Science Foundation of Longdong University(HXZK2488).
文摘Crude oil pollution is a significant global environmental challenge.The eastern Gansu Province on the Loess Plateau,an important agricultural region containing the Changqing Oilfield,is facing increasing crude oil contamination.Understanding how microbial communities respond to varying pollution levels is critical for developing effective bioremediation strategies.This study examined how different concentrations of crude oil affect soil properties and microbial communities in Qingyang City,eastern Gansu Province,China by comparing lightly polluted(1895.84-2696.54 mg/kg total petroleum hydrocarbons(TPH)),heavily polluted(4964.25-7153.61 mg/kg TPH),and uncontaminated(CK)soils.Results revealed that petroleum contamination significantly increased total organic carbon(TOC),pH,C:N:P ratio,and the activities of dehydrogenase(DHA)and polyphenol oxidase(PPO),while reducing total nitrogen(TN),available nitrogen(AN),total phosphorus(TP),available phosphorus(AP),available potassium(AK),soil organic matter(SOM),soil water content(SWC),the activities of urease(URE)and alkaline phosphatase(APA),and microbial alpha diversity(P<0.050).Light pollution(LP)soils demonstrated an increase in culturable microorganisms,whereas heavy pollution(HP)soils exhibited increased hydrocarbon-degrading microbes and higher expression of key functional genes,such as alkane monooxygenase(AlkB),cytochrome P450 alkane hydroxylases(P450),catechol 2,3-dioxygenase(C23O),and naphthalene dioxygenase(Nah)(P<0.050).Non-metric multidimensional scaling(NMDS)and redundancy analysis(RDA)indicated evident variations in microbial community structure across different oil contamination levels.LP soils were dominated by bacterial genera Pseudoxanthomonas and Solimonadaceae,whereas Pseudomonas,Nocardioides,and hydrocarbon-degrading genera(Marinobacter,Idiomarina,and Halomonas)were predominant in HP soils.The fungal genus Pseudallescheria exhibited the most pronounced abundance shift between LP and HP soils(P<0.050).Environmental factor analysis identified AN,SWC,TN,SOM,and alpha diversity indices(Shannon index and Chao1 index)as the key differentiators of CK soils,whereas the pollutant levels and metal content were characterized in HP soils.Hydrocarbon-degrading microbial abundance was a defining trait of HP soils.Metabolic pathway analysis revealed enhanced aromatic hydrocarbon degradation in HP soils,indicating microbial adaptation to severe contamination.These findings demonstrated that crude oil pollution suppressed soil nutrients while reshaping the structure and function of microbial communities.Pollution intensity directly affected microbial composition and degradation potential.This study offers valuable insights into microbial responses across contamination gradients and supports the development of targeted bioremediation strategies for oil-contaminated loess soils.
基金This work was supported in part by the Knowledge Innovation Engineering Action,the Chinese Academy of Sciences(KZCX2-SW-416)the National Natural Science Foundation of China(Grant No.20225722).
文摘The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach,and functional characteristics at community levels by using BIOLOG^(GN) system analysis method as well as two conventional methods(DHA and SIR).All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNA V_(3) high variable region universal primer)indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos,copper,especially some of their combinations had worse effects than their corresponding single factors.In addition,the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community.The effects of the single factors with lower concentrations on the communiy structure were weaker than those with higher concentrations.Moreover,the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors.The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOG^(GN)system were two relatively sensitive directors corresponding to the stress presented in this study.Between methamodophos and copper,there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities.The DHA of soil under the combined stresses was lower than that of the control and that under the single factors,and the BIOLOG^(GN) substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors.From all of above,the methamidophos,copper,especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure,richness,and the functional characteristics of microbial community.
