Microbial flocculants have become a hot spot in recent years. A bacterial strain with high flocculating activity was isolated from seawater samples. The strain was defined as Bacillus licheniformis dhs-40 by 16S rDNA ...Microbial flocculants have become a hot spot in recent years. A bacterial strain with high flocculating activity was isolated from seawater samples. The strain was defined as Bacillus licheniformis dhs-40 by 16S rDNA identification and Biolog test. Ultrasonication test confirmed the flocculating activity of the strain was both in fermentation supernatant and cell. According to flocculating activity curve, the ideal fermentation time for collecting flocculating active substances was two days. The flocculating activity of the strain was sensitive to pH. The strain could only preserve flocculating activity while pH varied from 7 to 11. However, it could preserve flocculating activity while temperature varied from -20℃to 100 ℃ Saccharide, protein, lipid, nucleic acids qualitative test and RNase, Proteinase K treatment confirmed the flocculating active substances were proteins. Their flocculating activities were insensitive to Proteinase K.展开更多
Human breast milk serves as a vital source of nutrition for infants,and it also plays a critical role in shaping the infant gut microbiota and establishing intestinal homeostasis.This process substantially impacts imm...Human breast milk serves as a vital source of nutrition for infants,and it also plays a critical role in shaping the infant gut microbiota and establishing intestinal homeostasis.This process substantially impacts immune function,neurodevelopment,and overall health.The noninvasive nature of breast milk collection makes it an ethical and accessible area for research,positioning it as a key focus for future studies.These future directions include the identification of novel bacteria combination,the establishment of comprehensive databases on infant microbiota,and the use of computational models to predict interactions between breast milk components and the gut microbiome.Additionally,the creation of diverse biological models and the establishment of infant stool banks will further enhance understanding of host-microbiome interactions and support disease prevention strategies.展开更多
文摘Microbial flocculants have become a hot spot in recent years. A bacterial strain with high flocculating activity was isolated from seawater samples. The strain was defined as Bacillus licheniformis dhs-40 by 16S rDNA identification and Biolog test. Ultrasonication test confirmed the flocculating activity of the strain was both in fermentation supernatant and cell. According to flocculating activity curve, the ideal fermentation time for collecting flocculating active substances was two days. The flocculating activity of the strain was sensitive to pH. The strain could only preserve flocculating activity while pH varied from 7 to 11. However, it could preserve flocculating activity while temperature varied from -20℃to 100 ℃ Saccharide, protein, lipid, nucleic acids qualitative test and RNase, Proteinase K treatment confirmed the flocculating active substances were proteins. Their flocculating activities were insensitive to Proteinase K.
基金supported by the National Natural Science Foundation(no.32470606)the Chinese Universities Scientific Fund(no.2024TC056)the 2115 Talent Development Program of China Agricultural University.
文摘Human breast milk serves as a vital source of nutrition for infants,and it also plays a critical role in shaping the infant gut microbiota and establishing intestinal homeostasis.This process substantially impacts immune function,neurodevelopment,and overall health.The noninvasive nature of breast milk collection makes it an ethical and accessible area for research,positioning it as a key focus for future studies.These future directions include the identification of novel bacteria combination,the establishment of comprehensive databases on infant microbiota,and the use of computational models to predict interactions between breast milk components and the gut microbiome.Additionally,the creation of diverse biological models and the establishment of infant stool banks will further enhance understanding of host-microbiome interactions and support disease prevention strategies.
