This study looks at the impact of climate change on the future distribution of Taxus baccata L.,a species under threat.It examines how altitudinal changes may influence distribution,projecting scenarios to 2100 using ...This study looks at the impact of climate change on the future distribution of Taxus baccata L.,a species under threat.It examines how altitudinal changes may influence distribution,projecting scenarios to 2100 using the SSPs 585 SSPs 245 scenarios in 20-year intervals.The results show a contraction in distribution in areas such as in Iceland and the United Kingdom,with certain extreme points disappearing.Simultaneously,new suitable areas are expected to emerge in select regions of Asia.The study underscores the significant changes anticipated in the distribution of T.baccata due to global climate change.It suggests that the threshold for addressing climate change on this particular species has been exceeded,and emphasizes the need for concerted efforts to mitigate and adapt to climate change impacts on ecosystems and organisms.As climate change affects various aspects of life,the study advocates for sector-wide plans.These would include efficient resource utilization,selecting genotypes for afforestation of this species with lower water requirements,incorporating climate change predictions into management plans,conserving biological and genetic diversity,and developing in-situ and exsitu conservation strategies.Anticipation of future climate changes and corresponding measures in response are crucial to minimizing the impact on this species.The study recommends establishing mixed forests composed of species resilient to a range of climate scenarios,thereby enhancing forest continuity across regions with varying degrees of climate impact.Genetic diversity is an important defense mechanism important to preserving it.Global climate change will result in significant alterations in the distribution of certain species,potentially causing population declines.Intervention is required to support the adaptation of vulnerable species,necessitating forward-looking strategies that anticipate shifts in their habitat suitability.This study emphasizes the implications of climate change for T.baccata and underscores the urgency of targeted conservation efforts to protect its populations and ensure long-term persistence.展开更多
[Objective] Abscisic acid （ABA）, a plant endogenous hormone, plays an important role in plant responses to adverse environments. This study was to explore the effects of exogenous ABA on the drought resistmlce of ap...[Objective] Abscisic acid （ABA）, a plant endogenous hormone, plays an important role in plant responses to adverse environments. This study was to explore the effects of exogenous ABA on the drought resistmlce of apple rootstocks under simulated drought condition induced by polyethylene glycol （PEG）. [Method] Apple （Malus baccata） seedlings were employed as test material. There were five treatments （T1-5） designed as ABA application rates at 0 （T1）, 25 （T2）, 50 （T3）, 75 （T4） and 100 （T5） μmol/L in the hydroponic experiments. The changes of the relative leaf water content （RLWC）, root activity, malondialdehyde （MDA） content, proline （Pro） content, superoxide dismutase （SOD） activity and peroxidase （POD） activity were assayed under PEG stress. All indices were analyzed by principal component analysis （PCA） to evaluate the optimal ABA concentration alleviating drought stress. [Result] The different concentration of ABA could increase Pro content and antioxidant enzyme activities, relieve the descent of RLWC and decrease MDA content. Low levels of ABA increased root activity, whereas the high concentrations （T3-T5） inhibited it. Statistical analysis based on the PCA indicated that the cumulative contribution rate of the first two principal components was raised to 96.457%, and the PCA in the T2 scored the highest. [Conclusion] The exogenous ABA could decrease the damage caused by drought stress to Malus baccata seedlings and enhance the ability of drought tolerance by increasing osmolyte content, anti- oxidative enzyme activity and reducing the level of membrane lipid peroxidation. The optimal concentration of ABA was 25 μmol/L.展开更多
In this study,several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol^(®)(paclitaxel)from culturable vascular stem cells(VSCs)of Taxus baccata,which is curr...In this study,several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol^(®)(paclitaxel)from culturable vascular stem cells(VSCs)of Taxus baccata,which is currently used as a successful cell line for paclitaxel production.An in situ product recovery(ISPR)technique was employed,which involved combining three commercial macro-porous resin beads(HP-20,XAD7HP and HP-2MG)with batch and semi-continuous cultivations of the T.baccata VSCs after adding methyl jasmonate(Me-JA)as an elicitor.The optimal resin combination resulted in 234±23 mg of paclitaxel per kg of fresh-weight cells,indicating a 13-fold improved yield compared to the control(with no resins)in batch cultivation.This resin treatment was further studied to evaluate the resins’removal capacity of reactive oxygen species(ROS),which can cause poor cell growth or reduce product synthesis.It was observed that the ISPR cultivations had fourfold less intracellular ROS concentration than that of the control;thus,a reduced ROS concentration established by the resin contributed to increased paclitaxel yield,contrary to previous studies.These paclitaxel yields are the highest reported to date using VSCs,and this scalable production method could be applied for a diverse range of similar compounds utilising plant cell culture.展开更多
Background The detrimental consequences of synthetic pharmaceuticals have generated interest in natural remedies.Balakata baccata(Roxb.)Esser a plant of ethnomedicinal significance,has been conventionally utilized for...Background The detrimental consequences of synthetic pharmaceuticals have generated interest in natural remedies.Balakata baccata(Roxb.)Esser a plant of ethnomedicinal significance,has been conventionally utilized for its anti-inflammatory and analgesic characteristics;nevertheless,its flowers are yet to be thoroughly investigated.Objective The current study examines the pharmacological potential of the methanol extract of B.baccata flowers(MEBBF)for antinociceptive,antipyretic,and anthelmintic effects.Methods MEBBF was assessed utilizing in vivo models,consisting of the acetic acid-induced writhing test,formalin-induced paw-licking test,yeast-induced pyrexia model,and Tubifex tubifex assay.GC-MS analysis was used to affirm the presence of bioactive chemicals,whereas molecular docking and ADMET analysis elucidated their mechanisms and safety profiles.Results The MEBBF demonstrated analgesic,antipyretic,and anthelmintic actions.It noticeably diminished pain responses,decreased temperature,and caused paralysis and mortality in Tubifex tubifex.Phytochemical analysis found flavonoids,phenolic compounds,and glycosides as principal bioactive constituents.Molecular docking of compounds from GC-MS of MEBBF demonstrated robust binding affinities with target proteins,while ADMET analysis suggested beneficial pharmacokinetic characteristics.Conclusion MEBBF has a high therapeutic potential for treating pain,fever,and helminthic diseases.Nevertheless,further research has to be done to substantiate these findings and confirm its curative applications.展开更多
The unsatisfactory bioavailability of polyphenols caused by poor solubility,instability and low absorption rate limits their application in functional food field.This study fabricated the nanoparticles based on chitos...The unsatisfactory bioavailability of polyphenols caused by poor solubility,instability and low absorption rate limits their application in functional food field.This study fabricated the nanoparticles based on chitosan and sodium alginate for encapsulation of the polyphenols isolated from Malus baccata(Linn.)(MBP-NPs)and investigated their gastrointestinal digestive and absorption characteristics.MBP-NPs with uniform sphere morphology had the average particle size of 611.3 nm and non-crystalline properties.During simulated gastrointestinal digestion in vitro,MBP-NPs exhibited the sustain release characteristics.Through administration of MBP-NPs to mice,the phenolic composition of MBP-NPs after gastrointestinal digestion was similar to that of undigested MBP,which suggested that the degradation of polyphenols during gastrointestinal digestion was successfully prevented.After small intestinal absorption,more polyphenols were detected in liver and spleen via participating in systemic circulation,thereby improving the bioavailability of MBP.MBP-NPs also promoted the production of short chain fatty acids for maintainance of intestinal health.Collectedly,these findings proved that the nanoparticles prepared based on CS and SA could be served as an effective strategy for encapsulation of MBP to potentially ameliorate their bioavailability.展开更多
基金supported by the TUBITAK YOK 100/2000 Scholarship。
文摘This study looks at the impact of climate change on the future distribution of Taxus baccata L.,a species under threat.It examines how altitudinal changes may influence distribution,projecting scenarios to 2100 using the SSPs 585 SSPs 245 scenarios in 20-year intervals.The results show a contraction in distribution in areas such as in Iceland and the United Kingdom,with certain extreme points disappearing.Simultaneously,new suitable areas are expected to emerge in select regions of Asia.The study underscores the significant changes anticipated in the distribution of T.baccata due to global climate change.It suggests that the threshold for addressing climate change on this particular species has been exceeded,and emphasizes the need for concerted efforts to mitigate and adapt to climate change impacts on ecosystems and organisms.As climate change affects various aspects of life,the study advocates for sector-wide plans.These would include efficient resource utilization,selecting genotypes for afforestation of this species with lower water requirements,incorporating climate change predictions into management plans,conserving biological and genetic diversity,and developing in-situ and exsitu conservation strategies.Anticipation of future climate changes and corresponding measures in response are crucial to minimizing the impact on this species.The study recommends establishing mixed forests composed of species resilient to a range of climate scenarios,thereby enhancing forest continuity across regions with varying degrees of climate impact.Genetic diversity is an important defense mechanism important to preserving it.Global climate change will result in significant alterations in the distribution of certain species,potentially causing population declines.Intervention is required to support the adaptation of vulnerable species,necessitating forward-looking strategies that anticipate shifts in their habitat suitability.This study emphasizes the implications of climate change for T.baccata and underscores the urgency of targeted conservation efforts to protect its populations and ensure long-term persistence.
文摘[Objective] Abscisic acid （ABA）, a plant endogenous hormone, plays an important role in plant responses to adverse environments. This study was to explore the effects of exogenous ABA on the drought resistmlce of apple rootstocks under simulated drought condition induced by polyethylene glycol （PEG）. [Method] Apple （Malus baccata） seedlings were employed as test material. There were five treatments （T1-5） designed as ABA application rates at 0 （T1）, 25 （T2）, 50 （T3）, 75 （T4） and 100 （T5） μmol/L in the hydroponic experiments. The changes of the relative leaf water content （RLWC）, root activity, malondialdehyde （MDA） content, proline （Pro） content, superoxide dismutase （SOD） activity and peroxidase （POD） activity were assayed under PEG stress. All indices were analyzed by principal component analysis （PCA） to evaluate the optimal ABA concentration alleviating drought stress. [Result] The different concentration of ABA could increase Pro content and antioxidant enzyme activities, relieve the descent of RLWC and decrease MDA content. Low levels of ABA increased root activity, whereas the high concentrations （T3-T5） inhibited it. Statistical analysis based on the PCA indicated that the cumulative contribution rate of the first two principal components was raised to 96.457%, and the PCA in the T2 scored the highest. [Conclusion] The exogenous ABA could decrease the damage caused by drought stress to Malus baccata seedlings and enhance the ability of drought tolerance by increasing osmolyte content, anti- oxidative enzyme activity and reducing the level of membrane lipid peroxidation. The optimal concentration of ABA was 25 μmol/L.
基金supported by the Mexican government dependence CONACyT(Mexican National Council for Science and Technology)Scholarship reference CVU:537962 for JHSG,and CVU:537957 for MVCsupported by the ANII(Uruguayan National Agency for Research and Innovation)Scholarship reference:POS_NAC_2017_1_140229 for MR+2 种基金supported by a fellowship from the Universitat Pompeu Fabra.The Royal Society(Grant Number RSG\R1\180345)The British Council(Grant Number:527429894)supported by the Engineering and Physical Sciences Research Council(Grant number EP/R513209/1).
文摘In this study,several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol^(®)(paclitaxel)from culturable vascular stem cells(VSCs)of Taxus baccata,which is currently used as a successful cell line for paclitaxel production.An in situ product recovery(ISPR)technique was employed,which involved combining three commercial macro-porous resin beads(HP-20,XAD7HP and HP-2MG)with batch and semi-continuous cultivations of the T.baccata VSCs after adding methyl jasmonate(Me-JA)as an elicitor.The optimal resin combination resulted in 234±23 mg of paclitaxel per kg of fresh-weight cells,indicating a 13-fold improved yield compared to the control(with no resins)in batch cultivation.This resin treatment was further studied to evaluate the resins’removal capacity of reactive oxygen species(ROS),which can cause poor cell growth or reduce product synthesis.It was observed that the ISPR cultivations had fourfold less intracellular ROS concentration than that of the control;thus,a reduced ROS concentration established by the resin contributed to increased paclitaxel yield,contrary to previous studies.These paclitaxel yields are the highest reported to date using VSCs,and this scalable production method could be applied for a diverse range of similar compounds utilising plant cell culture.
文摘Background The detrimental consequences of synthetic pharmaceuticals have generated interest in natural remedies.Balakata baccata(Roxb.)Esser a plant of ethnomedicinal significance,has been conventionally utilized for its anti-inflammatory and analgesic characteristics;nevertheless,its flowers are yet to be thoroughly investigated.Objective The current study examines the pharmacological potential of the methanol extract of B.baccata flowers(MEBBF)for antinociceptive,antipyretic,and anthelmintic effects.Methods MEBBF was assessed utilizing in vivo models,consisting of the acetic acid-induced writhing test,formalin-induced paw-licking test,yeast-induced pyrexia model,and Tubifex tubifex assay.GC-MS analysis was used to affirm the presence of bioactive chemicals,whereas molecular docking and ADMET analysis elucidated their mechanisms and safety profiles.Results The MEBBF demonstrated analgesic,antipyretic,and anthelmintic actions.It noticeably diminished pain responses,decreased temperature,and caused paralysis and mortality in Tubifex tubifex.Phytochemical analysis found flavonoids,phenolic compounds,and glycosides as principal bioactive constituents.Molecular docking of compounds from GC-MS of MEBBF demonstrated robust binding affinities with target proteins,while ADMET analysis suggested beneficial pharmacokinetic characteristics.Conclusion MEBBF has a high therapeutic potential for treating pain,fever,and helminthic diseases.Nevertheless,further research has to be done to substantiate these findings and confirm its curative applications.
基金funded by National Natural Science Foundation of China(31972080,31500652)Science and Technology Project of Hebei Education Department(BJK2023118)Natural Science Foundation of Hebei Province(C2024203004,C2023203003).
文摘The unsatisfactory bioavailability of polyphenols caused by poor solubility,instability and low absorption rate limits their application in functional food field.This study fabricated the nanoparticles based on chitosan and sodium alginate for encapsulation of the polyphenols isolated from Malus baccata(Linn.)(MBP-NPs)and investigated their gastrointestinal digestive and absorption characteristics.MBP-NPs with uniform sphere morphology had the average particle size of 611.3 nm and non-crystalline properties.During simulated gastrointestinal digestion in vitro,MBP-NPs exhibited the sustain release characteristics.Through administration of MBP-NPs to mice,the phenolic composition of MBP-NPs after gastrointestinal digestion was similar to that of undigested MBP,which suggested that the degradation of polyphenols during gastrointestinal digestion was successfully prevented.After small intestinal absorption,more polyphenols were detected in liver and spleen via participating in systemic circulation,thereby improving the bioavailability of MBP.MBP-NPs also promoted the production of short chain fatty acids for maintainance of intestinal health.Collectedly,these findings proved that the nanoparticles prepared based on CS and SA could be served as an effective strategy for encapsulation of MBP to potentially ameliorate their bioavailability.
