在叶片中合成由FLOWERING LOCUS T(FT)编码的成花素蛋白通过韧皮部运输到顶端分生组织(SAM),与b ZIP转录因子FD相互作用形成复合物,激活花分生组织身份基因的表达,从而调节植物开花和其他的一些生物学过程。本研究以棉花全基因组数据库...在叶片中合成由FLOWERING LOCUS T(FT)编码的成花素蛋白通过韧皮部运输到顶端分生组织(SAM),与b ZIP转录因子FD相互作用形成复合物,激活花分生组织身份基因的表达,从而调节植物开花和其他的一些生物学过程。本研究以棉花全基因组数据库为基础,发掘并得到18个棉花FD基因的序列并确定它们在各基因组染色体上的位置。生物信息学分析显示,棉花FD蛋白质分子量在15.69~28.24 k D之间,理论等电点在9.24~10.38之间,是一种非分泌性蛋白;亚细胞定位预测显示,棉花FD蛋白分布于细胞核上,是典型的核蛋白;氨基酸序列分析表明,棉花FD是一种亲水性蛋白,还存在着糖基化位点和磷酸化位点以及4个保守基序;进化分析表明,棉花FD1和FD2与葡萄Vv FD基因亲缘关系最近。组织表达分析表明,陆地棉10个Gh FD基因在不同组织中具有表达特征多样性,Gh FD5-D在纤维中的表达量最高,其余Gh FD基因均在SAM中的表达量最高,不同的表达特征表明它们可能具有不同的功能。这些结果为进一步解析棉花FD基因的功能和作用机理积累了有价值的资料。展开更多
Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide.The plant disease cycle involves sexual reproduction,with the perithecium playing a crucial role in overwintering and the disch...Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide.The plant disease cycle involves sexual reproduction,with the perithecium playing a crucial role in overwintering and the discharge of ascospores.Although fatty acid biosynthesis and metabolism are linked to perithecium formation and ascospore discharge,the regulation of these processes remains largely unknown.In this study,we identified and characterized FgBZIP16,as a basic leucine zipper(b-ZIP)transcription factor,in F.graminearum.Targeted gene deletion revealed that FgBzip16 is important for vegetative growth,asexual/sexual development,and plant infection.Cytological observations revealed that FgBzip16 was localized in the nucleus during the hyphal and conidial stages.FgBzip16 is essential for ascospore discharge,with transcriptomics and molecular biology showing it binds to the promoter of its target genes FGSG_05321 and FGSG_03244,which regulate ascospore discharge by encoding fatty acid synthase subunit alpha-reductase and enoyl hydratase,respectively.Altogether,these results constitute the first report of the specific functions associated with b-ZIP transcription factor FgBzip16,linking its regulatory roles to fungal development,fatty acid accumulation,and metabolism,host penetration,and pathogenicity of F.graminearum.展开更多
文摘在叶片中合成由FLOWERING LOCUS T(FT)编码的成花素蛋白通过韧皮部运输到顶端分生组织(SAM),与b ZIP转录因子FD相互作用形成复合物,激活花分生组织身份基因的表达,从而调节植物开花和其他的一些生物学过程。本研究以棉花全基因组数据库为基础,发掘并得到18个棉花FD基因的序列并确定它们在各基因组染色体上的位置。生物信息学分析显示,棉花FD蛋白质分子量在15.69~28.24 k D之间,理论等电点在9.24~10.38之间,是一种非分泌性蛋白;亚细胞定位预测显示,棉花FD蛋白分布于细胞核上,是典型的核蛋白;氨基酸序列分析表明,棉花FD是一种亲水性蛋白,还存在着糖基化位点和磷酸化位点以及4个保守基序;进化分析表明,棉花FD1和FD2与葡萄Vv FD基因亲缘关系最近。组织表达分析表明,陆地棉10个Gh FD基因在不同组织中具有表达特征多样性,Gh FD5-D在纤维中的表达量最高,其余Gh FD基因均在SAM中的表达量最高,不同的表达特征表明它们可能具有不同的功能。这些结果为进一步解析棉花FD基因的功能和作用机理积累了有价值的资料。
基金supported by the National Natural Science Foundation of China(Grant No:32101530)the Natural Science Foundation of Jiangsu Province(Grant No:BK20200953)+2 种基金the Open Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests(Grant No:SKLOF 202202)the Foundation of Key Technology Research Project of Henan Province(Grant No:222102110031)the China Postdoctoral Science Foundation(Grant No:2022M712886).
文摘Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide.The plant disease cycle involves sexual reproduction,with the perithecium playing a crucial role in overwintering and the discharge of ascospores.Although fatty acid biosynthesis and metabolism are linked to perithecium formation and ascospore discharge,the regulation of these processes remains largely unknown.In this study,we identified and characterized FgBZIP16,as a basic leucine zipper(b-ZIP)transcription factor,in F.graminearum.Targeted gene deletion revealed that FgBzip16 is important for vegetative growth,asexual/sexual development,and plant infection.Cytological observations revealed that FgBzip16 was localized in the nucleus during the hyphal and conidial stages.FgBzip16 is essential for ascospore discharge,with transcriptomics and molecular biology showing it binds to the promoter of its target genes FGSG_05321 and FGSG_03244,which regulate ascospore discharge by encoding fatty acid synthase subunit alpha-reductase and enoyl hydratase,respectively.Altogether,these results constitute the first report of the specific functions associated with b-ZIP transcription factor FgBzip16,linking its regulatory roles to fungal development,fatty acid accumulation,and metabolism,host penetration,and pathogenicity of F.graminearum.
