Objective To study the hepatitis B virus （HBV） vertical transmission via infected spermatozoa. Methods Eighteen male patients with HBV infection who underwent in vitro fertilization （IVF） were studied, 5 HBV negat...Objective To study the hepatitis B virus （HBV） vertical transmission via infected spermatozoa. Methods Eighteen male patients with HBV infection who underwent in vitro fertilization （IVF） were studied, 5 HBV negative patients were selected as the control. Fluorescence in situ hybridization （FISH） analysis using the partial-length HBV DNA as the hybridization probe was performed to explore the existence of HBV DNA in the sperm and in the host embryonic genome. Results FISH showed that 5 of 18 patients＇ sperm presented positive signals and 2 of 18 embryos presented positive signals, while no positive signals were found in control group. Conclusion The HBV DNA was found in human sperm and embryos of HBV patients. These results provide direct evidence that HBV DNA could transmit to foetus via human infected spermatozoa.展开更多
The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activ...The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.展开更多
To investigate the evaluation of the early follicular phase serum inhibin B(INHB) levels and anti-mullerian hormone(AMH) as indicator of ovarian reserve and the responsibility of COS.361 cases in COS group and 1,792 c...To investigate the evaluation of the early follicular phase serum inhibin B(INHB) levels and anti-mullerian hormone(AMH) as indicator of ovarian reserve and the responsibility of COS.361 cases in COS group and 1,792 cases in routine treatment of infertility.The number of retrieved oocytes correlate positively with INHB serum concentrations.While INHB≤30 pg/ml,the number of retrieved oocytes is 2 to 3(47%).While FSH≤10 IU/L,INHB>30 pg/ml,86% retrieved oocytes;INHB <30 pg/ml,only 7% retrieved oocytes.The number of retrieved oocytes,quality embryos,pregnancy rate correlate positively with INHB serum concentrations(r= 0.323,0.404,0.323,0.246, respectively,P<0.05).The effect of INHB and AMH for predicting ovarian reserve and treatment options in assisted reproduction,are being as strong predictive factor,better than FSH.展开更多
通过盆栽试验,研究了UV-B增强下不同施硅量和硅肥种类对水稻生长及甲烷(CH4)排放的影响.UV-B辐射设2个水平,即对照(自然光,A)和增强20%(E);硅肥设4个水平,即Si0(不施硅,0 kg Si O2·hm-2)、Si1(硅酸钠,100 kg Si O2·hm-2)、Si2...通过盆栽试验,研究了UV-B增强下不同施硅量和硅肥种类对水稻生长及甲烷(CH4)排放的影响.UV-B辐射设2个水平,即对照(自然光,A)和增强20%(E);硅肥设4个水平,即Si0(不施硅,0 kg Si O2·hm-2)、Si1(硅酸钠,100 kg Si O2·hm-2)、Si2(硅酸钠,200kg Si O2·hm-2)和Si3(钢渣硅肥,200 kg Si O2·hm-2).结果表明:施硅能缓解UV-B增强对水稻生长的抑制作用,使分蘖数、叶绿素含量、地上部和地下部生物量增加.施硅对水稻生长的促进作用随施硅量(硅酸钠)的增加而增加,钢渣硅肥的促进作用大于硅酸钠.UV-B增强可提高稻田CH4的排放通量和累积排放量,施硅显著降低CH4的排放通量和累积排放量,且CH4排放随施硅量的增加而减少.在施硅量相同的情况下,钢渣硅肥的减排效果优于硅酸钠.表明在水稻生产中,施用钢渣硅肥不仅能实现废弃物利用,而且可有效降低UV-B增强下CH4的排放量.展开更多
基金funded by a grant from the Natural Science Foundation of Hubei Province (No. 2001AA310B06)from the Bureau of Science and Technology of Shiyan City (No. 2001D19)
文摘Objective To study the hepatitis B virus （HBV） vertical transmission via infected spermatozoa. Methods Eighteen male patients with HBV infection who underwent in vitro fertilization （IVF） were studied, 5 HBV negative patients were selected as the control. Fluorescence in situ hybridization （FISH） analysis using the partial-length HBV DNA as the hybridization probe was performed to explore the existence of HBV DNA in the sperm and in the host embryonic genome. Results FISH showed that 5 of 18 patients＇ sperm presented positive signals and 2 of 18 embryos presented positive signals, while no positive signals were found in control group. Conclusion The HBV DNA was found in human sperm and embryos of HBV patients. These results provide direct evidence that HBV DNA could transmit to foetus via human infected spermatozoa.
文摘The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.
文摘To investigate the evaluation of the early follicular phase serum inhibin B(INHB) levels and anti-mullerian hormone(AMH) as indicator of ovarian reserve and the responsibility of COS.361 cases in COS group and 1,792 cases in routine treatment of infertility.The number of retrieved oocytes correlate positively with INHB serum concentrations.While INHB≤30 pg/ml,the number of retrieved oocytes is 2 to 3(47%).While FSH≤10 IU/L,INHB>30 pg/ml,86% retrieved oocytes;INHB <30 pg/ml,only 7% retrieved oocytes.The number of retrieved oocytes,quality embryos,pregnancy rate correlate positively with INHB serum concentrations(r= 0.323,0.404,0.323,0.246, respectively,P<0.05).The effect of INHB and AMH for predicting ovarian reserve and treatment options in assisted reproduction,are being as strong predictive factor,better than FSH.
文摘通过盆栽试验,研究了UV-B增强下不同施硅量和硅肥种类对水稻生长及甲烷(CH4)排放的影响.UV-B辐射设2个水平,即对照(自然光,A)和增强20%(E);硅肥设4个水平,即Si0(不施硅,0 kg Si O2·hm-2)、Si1(硅酸钠,100 kg Si O2·hm-2)、Si2(硅酸钠,200kg Si O2·hm-2)和Si3(钢渣硅肥,200 kg Si O2·hm-2).结果表明:施硅能缓解UV-B增强对水稻生长的抑制作用,使分蘖数、叶绿素含量、地上部和地下部生物量增加.施硅对水稻生长的促进作用随施硅量(硅酸钠)的增加而增加,钢渣硅肥的促进作用大于硅酸钠.UV-B增强可提高稻田CH4的排放通量和累积排放量,施硅显著降低CH4的排放通量和累积排放量,且CH4排放随施硅量的增加而减少.在施硅量相同的情况下,钢渣硅肥的减排效果优于硅酸钠.表明在水稻生产中,施用钢渣硅肥不仅能实现废弃物利用,而且可有效降低UV-B增强下CH4的排放量.
