Brachylaena huillensis (Asteraceae) is a threatened resourceful timber tree species. B. huillensis regenerates only through seeds. However, the seeds have poor germination rate and are also not obtainable. Developing ...Brachylaena huillensis (Asteraceae) is a threatened resourceful timber tree species. B. huillensis regenerates only through seeds. However, the seeds have poor germination rate and are also not obtainable. Developing tissue culture techniques for B. huillensis will permit the application of biotechnology to its propagation and provide alternative method for its regeneration. The current study was conducted to investigate the effect of antioxidant ascorbic acid in controlling lethal browning caused by oxidized phenols in in vitro culture of Brachylaena huillensis using nodal segments. The treatments included four levels of ascorbic acid (0, 50, 100, 150, 200, & 250 mg/litre) supplied into basal woody plant medium supplemented with Benzylaminopurine (BAP). The results of the current study revealed that production of phenolic compounds of explants was significantly controlled by incorporating higher levels of ascorbic acid into the medium. The best control was achieved by supplying 200-250 mg/litre of ascorbic acid in the woody plant medium supplemented with BAP.展开更多
Introduction Efective labour induction is marked by the onset of uterine contractions,progressive cervical dilation,and efacement,culminating in a vaginal birth without matemal or fetal complications.The success of in...Introduction Efective labour induction is marked by the onset of uterine contractions,progressive cervical dilation,and efacement,culminating in a vaginal birth without matemal or fetal complications.The success of induction largely depends on cenvical ripening.Isosorbide mononitrate(ISMN),a nitric axide donor,has shown potential for cervical ripening.faciitating labour induction by promoting cervical softening,efacement and dilation.This study evaluates the eficacy and safety of ISMN for cervical ripening before labour induction at term or post-term.Methods In this double-binded randomised controlled trial,160 primigravidas with an unripe cenix(Bishop score<6)undergoing labour induction at term or post-term were enolled.The ISMN group received 40mg of intravaginal ISMN in the posterior formix every 4hours,up to four doses.The placebo group received intravaginal pridoxine placebos on the same schedule.The primary outcome measured was the duration from labour induction to placental dllvery.Secondary outcomes included caesarean section rates.Results The ISMN group had a ststically significant shorter induction-to-delivery time compared with the placebo group(20.5714.64hours VS 23.15+5.13hours,p=0.01).Addionally,the time from the first dose to dellvery was reduced in the ISMN group.The caesarean section rate was lower in the ISMN group(29%VS 41%,p=0.17),though this dfferenrce was not stitially significant.Conclusion ISMN is an efctive and safe option for cerical ripening,poentally shortening the labour duration.展开更多
文摘Brachylaena huillensis (Asteraceae) is a threatened resourceful timber tree species. B. huillensis regenerates only through seeds. However, the seeds have poor germination rate and are also not obtainable. Developing tissue culture techniques for B. huillensis will permit the application of biotechnology to its propagation and provide alternative method for its regeneration. The current study was conducted to investigate the effect of antioxidant ascorbic acid in controlling lethal browning caused by oxidized phenols in in vitro culture of Brachylaena huillensis using nodal segments. The treatments included four levels of ascorbic acid (0, 50, 100, 150, 200, & 250 mg/litre) supplied into basal woody plant medium supplemented with Benzylaminopurine (BAP). The results of the current study revealed that production of phenolic compounds of explants was significantly controlled by incorporating higher levels of ascorbic acid into the medium. The best control was achieved by supplying 200-250 mg/litre of ascorbic acid in the woody plant medium supplemented with BAP.
文摘Introduction Efective labour induction is marked by the onset of uterine contractions,progressive cervical dilation,and efacement,culminating in a vaginal birth without matemal or fetal complications.The success of induction largely depends on cenvical ripening.Isosorbide mononitrate(ISMN),a nitric axide donor,has shown potential for cervical ripening.faciitating labour induction by promoting cervical softening,efacement and dilation.This study evaluates the eficacy and safety of ISMN for cervical ripening before labour induction at term or post-term.Methods In this double-binded randomised controlled trial,160 primigravidas with an unripe cenix(Bishop score<6)undergoing labour induction at term or post-term were enolled.The ISMN group received 40mg of intravaginal ISMN in the posterior formix every 4hours,up to four doses.The placebo group received intravaginal pridoxine placebos on the same schedule.The primary outcome measured was the duration from labour induction to placental dllvery.Secondary outcomes included caesarean section rates.Results The ISMN group had a ststically significant shorter induction-to-delivery time compared with the placebo group(20.5714.64hours VS 23.15+5.13hours,p=0.01).Addionally,the time from the first dose to dellvery was reduced in the ISMN group.The caesarean section rate was lower in the ISMN group(29%VS 41%,p=0.17),though this dfferenrce was not stitially significant.Conclusion ISMN is an efctive and safe option for cerical ripening,poentally shortening the labour duration.
